• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1070-1074
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• 1999

Growth and feeding studies were conducted to determine effects of hazelnut kernel oil meal (HKOM) on growth performance (as protein efficiency), and milk production and composition. In the growth study, 24 individually fed Karayaka lambs (4 mo. and 25.55 kg LW) were used to determine protein efficiency calculated using the Slope Ratio Technique. In the feeding trial, 4 Jersey cows were arranged in $4{\times}4$ Latin squares experiment to measure effects of diets containing HKOM, soybean meal (SBM) corn gluten meal (CGU) and urea (U) on milk production and composition. Protein efficiencies for HKOM, SBM and CGM were found as $1.342{\pm}0.499$, $0.879{\pm}0.488$ and $1.833{\pm}0.893$, respectively. Milk production for the cows consuming concentrates, containing HKOM, SBM, CGM and U, were $13.97{\pm}0.99$, $13.20{\pm}1.09$, $14.86{\pm}0.68 $ and $13.06{\pm}1.23kg/d$ (p<0.01), respectively. There were no differences (p>0.05) among diets for milk protein content were statistically different (p<0.05), although milk DM and fat percentage as well as milk solids-not-fat and lactose percentage (p<0.01). The highest DM intake was associated with the U diet, intake was intermediate with the SBM and HKOM diets, and the lowest with CGM diet (p<0.05). In conclusion, there data may indicate that the HKOM is useful in diets as a protein source for growing ruminants and lactating cows.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1585-1591
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• 2008

The aim of the present work was to monitor metabolic and hormonal profiles in newborn kids, born from dams fed diets with low or high levels of energy requirements. Starting from the last month of pregnancy, 14 goats were randomly allocated to two groups: Group LD (low diet) and Group HD (high diet) that received a diet that covered 80% and 140% of their energy requirement, respectively. At delivery, the kids were weighed and a blood sample was taken before they suckled colostrum (Time 0) and 1, 2, 3, 12 and 24 h after they started suckling. Plasma insulin, IGF-I, glucose, fT3 and fT4 concentrations were not influenced by the dietary treatments, but a significant effect of time was observed as they progressively increased during the first 12 h of life. Plasma cholesterol, triglyceride, albumin, globulin and total protein plasma concentrations were significantly higher in Group HD than those of Group LD. In Group HD, cortisol concentrations were significantly lower than those of Group LD. Positive correlations were observed between LW and IGF-I (r = 0.71; p<0.05), plasma insulin and glucose (r = 0.79; p<0.05) and total protein and globulin concentrations (r = 0.97; p<0.001). Our results show that perinatal nutrition affects newborn goat kids' metabolic and hormonal profile.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.818-824
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• 2014

The nutrient composition of pasture, voluntary intake and digestibility of diet ingested by goats grazing on an introduced Leymus chinensis pasture were measured across spring (May), summer (July), autumn (October) and winter (March). In each season, 12 Inner Mongolian Cashmere goats (6 wethers and 6 does with an average live weight of $22.2{\pm}1.3$ kg and $19.5{\pm}0.8$ kg, respectively) were used to graze on a 2 hectares size paddock. Diet selection was observed and the plant parts selected by grazing goats and whole plant L. chinensis were sampled simultaneously. The alkane pair $C_{32}:C_{33}$ and $C_{36}$ were used to estimate intake and digestibility, respectively. The results showed that the plant parts selected by goats had higher crude protein (CP) and lower acid detergent fiber (ADF) and neutral detergent fiber (NDF) than the whole plant, especially in the autumn and winter. The voluntary intake of dry matter (DM), CP, ADF, NDF, and metabolizable energy (ME) by goats was highest in summer (p<0.05). The goats ingested more CP, ME, and less ADF in spring than in autumn (p<0.05). The intakes of DM, CP, and ME were lowest in winter (p<0.05). There were significant differences in nutrient intake between wethers and does in each season, except for the ADF and ME intake per metabolic weight ($LW^{0.75}$). The nutrient digestibilities were higher in spring and summer, and decreased significantly during the autumn and winter (p<0.05). Goats, especially wethers, had a relative constant NDF digestibility across seasons, however, the apparent digestibility of CP in both wethers and does, decreased to negative values in winter. The grazing goats experienced relatively sufficient nutrients supply in spring and summer, and a severe deficiency of CP and ME in winter.

• 대한수의학회지
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• 제56권2호
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• pp.125-127
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• 2016

Gradual mortality of look down fish (Selene vomer) was observed in a private aquarium in Seoul, showing abnormal swimming behavior and lethargy. A bacterial pathogen from kidney was cultured, identified, and confirmed as Vibrio harveyi using Vitek System 2 and 16S rRNA gene sequencing. A predominant bacterial strain, SNUVh-LW2 was proved to be most closely related to isolates from China by phylogenetic analysis with minimum evolution method. Also, tetracycline was considered as the most sensitive antibiotic agent via antibiotic susceptibility test. The group of fish was treated according to the diagnostic result and no more mortality was observed.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.321-327
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• 1985

색소를 형성하지 못하는 Serratia sp. LW-1 균주의 균체의 protease생성조건을 검토하고 효소를 간단한 방법으로 정제하였다. 효소 생산을 위한 최적온도는 brain heart infusion배지에서 $25^{\circ}C$이었으며, 배양후 76-80시간에 최고의 균체의 효소활성을 나타냈다. Aeration효과는 5$\ell$용 flask에 배지량을 1$\ell$주입하여 180 cycles/min으로 진탕배양 하였을 때가 aeration하지 않았을 경우보다 약 8배의 균체의 효소생산을 보였다. 효소의 정 제는 ammonium sulfate침 전, ammonium sulfate분별염석 및 두번의 DEAE-cellulose column 크로마토그라피에 의하여 수행하였으며, 정제된 효소는 정제도가 약 100배, 회수율이 16%이었다. 정제된 효소는 analytical ultracentrifuge pattern에서 단일 단백질로 나타났으며 최고 활성을 나타내는 pH는 vitamin free casein을 substrate로 사용하였을 때 pH 8.5-9.5이었고 최적온도는 4$0^{\circ}C$ 근처이었다

• 한국운동역학회지
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• 제24권4호
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• pp.425-433
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• 2014

The purpose of the study was to investigate the effect of CAD (Computer Aided Design) based alpine mono-ski bucket design on disabled ski athletes' kinematic characteristics. Two national team ski athletes with LW11 disabilities (Locomotion Winter Classification) category for sit ski participated in both pre and post experiment. Both of the subjects performed 3 trials of carved turn on a ski slope under two conditions. Where, subject "A" performed pre experiment with personal bucket and post experiment with the newly developed CAD based bucket whereas, Subject "B" as control subject performed both pre and post experiment with his personal bucket. For the experiment, 24 Infrared cameras were positioned on the ski slope which covered the path of the ski turn. Also, motion capture suit with reflective markers were worn by both subjects. In the result, decrement in medial/lateral displacement of COM, anterior/posterior displacement of COM, flexion/extension angle of trunk as well as velocity losing rate of COM was observed in subject "A" when using the newly developed CAD based bucket. In contrast, no larger effect on performance was observed when using personal buckets. In conclusion, the findings obtained from the study indicated effectiveness of newly developed CAD based bucket by reducing excessive movement of hip and trunk which is an important factor to perform an effective turn.

• 대한수의학회지
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• 제56권1호
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• pp.45-46
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• 2016

For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1266-1272
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• 2019

A bacterial strain, labeled $UR11^T$, was isolated from green alga Ulva pertusa collected from Jeju Island, Korea. $UR11^T$ was identified as a gram-negative, rod-shaped, motile by gliding and aerobic bacterial strain with yellow colonies on R2A plates. The strain $UR11^T$ grew over at a temperature range of $10^{\circ}C$ to $30^{\circ}C$ (optimally at $25^{\circ}C$), a pH range of 6.0-11 (optimally at pH 7.0) and a Nacl range of 0.5-5% Nacl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain $UR11^T$ was a member of the genus Flavobacterium. Strain $UR11^T$ shared close similarity with F. jejuensis $EC11^T$ (98.0%) F. jumunjinense $HME7102^T$ (96.1%), F. haoranii $LQY-7^T$ (95.3%), F. dongtanense $LW30^T$ (95.1%), and F. ahnfeltiae 10Alg $130^T$(94.9%). The major fatty acids (>5%) were $iso-C_{15:0}$ (33.9%), $iso-C_{15:1}$ G (12.4%), $iso-C_{17:0}$ 3-OH (9.0%), $isoC_{16:0}$ (7.0%) and $iso-C_{15:0}$ 3-OH (6.3%). The major polar lipids were phosphatidylethanolamine, seven unknown aminolipids, two unknown aminopolarlipids and two unknown lipids. DNA-DNA hybridization value was 58% at strain $UR11^T$ with F. jejuensis $EC11^T$. Based on phenotypic, chemotaxonomic and phylogenetic evidence, strain $UR11^T$ represents a novel species of the genus Flavobacterium, for which the name Flavobacterium jocheonensis sp. nov. is proposed. The type strain is Flavobacterium jocheonensis is $UR11^T$ (=KCTC $52377^T$ =JCM $31512^T$).

• 한국지반신소재학회논문집
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• 제21권3호
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• pp.29-39
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• 2022

본 연구에서는 용탈 저감 효과가 있는 합성폴리머 실리카졸의 그라우트 성능을 평가하고, 기존 물유리계 SGR 주입재와 비교한 내용을 다루었다. 강도 및 내구성에 대한 실내실험 결과 합성폴리머 실리카졸는 물유리계 주입재 대비 2배 이상 높은 일축압축강도, 수축률 및 투수성에서 현저히 낮은 값을 보여주었고, pH는 먹는 물 수질기준인 8.5 이하였다. 용탈시험결과 합성폴리머 실리카졸의 Na₂O 용출량은 물유리계 주입재 대비 3~4배 작게 측정되었다. 이러한 결과로 보아 합성폴리머 실리카졸은 기존 물유리계 주입재보다 더 우수한 내구성 및 차수성을 가지는 것으로 판단된다.

• Korean Chemical Engineering Research
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• 제50권4호
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• pp.666-671
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• 2012

가스 하이드레이트는 고압 및 저온의 조건에서 주체 분자인 물의 수소 결합에 의해 형성된 격자 내로 저분자량의 기체 분자가 포집되어 있는 고체의 함유 화합물이다. 심해저 퇴적층과 영구 동토지역에 매장되어 있는 막대한 양의 천연가스 하이드레이트는 미래 청정 에너지원으로 주목받고 있다. 우리나라의 경우에도 동해안 울릉분지 부근 심해저에 천연가스 하이드레이트의 부존 가능성을 확인하였다. 이러한 심해저 천연가스 하이드레이트의 개발/생산을 위해서는 천연가스 하이드레이트의 상평형, 생성/해리 속도 등의 물리적 특성 뿐만 아니라 해리와 관련된 열물성에 대한 정보가 매우 중요하다. 따라서, 이 논문에서는 고압 마이크로 시차 주사 열량계를 이용하여 천연가스 하이드레이트의 주성분인 메탄, 에탄, 프로판 하이드레이트의 해리 엔탈피를 측정하였다. 메탄 하이드레이트의 경우 92.3 bar의 압력 조건에서 502.1 J/g-water, 437.3 J/g-hydrate, 54.2 kJ/mol-gas의 해리 엔탈피 값을 가졌으며, 해리 온도는 285.66 K이었다. 에탄 하이드레이트의 경우 18.7 bar에서 해리 엔탈피는 534.5 J/g-water, 438.8 J/g-hydrate, 73.8 kJ/mol-gas이었으며, 이때 해리 온도는 283.85 K이었다. 마지막으로 프로판 하이드레이트의 경우 압력이 3.2 bar일 때 해리 엔탈피는 417.2 J/g-water, 363.8 J/g-hydrate, 127.7 kJ/mol-gas 임을 알 수 있었고, 276.30 K에서 해리되는 것을 확인하였다. 또한, 해당 압력에서 각 기체의 가스 하이드레이트의 해리 엔탈피 측정과정에서 얻은 해리 온도를 문헌상의 가스 하이드레이트 3상(하이드레이트상(H)-물상(Lw)-기상(V)) 평형 데이터와 비교해 보면 거의 유사함을 알 수 있었다. 따라서, 고압 마이크로 시차 주사 열량계를 이용하여 가스 하이드레이트의 정확한 해리 엔탈피 측정 뿐만 아니라 가스 하이드레이트의 3상 평형도 함께 측정 가능함을 확인하였다.