대한수의학회지 (Korean Journal of Veterinary Research)

  • 제56권2호
  • /
  • Pages.125-127
  • /
  • 2016
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

Isolation and molecular identification of Vibrio harveyi from the look down fish (Selene vomer)

  • Jun, Jin-Woo (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Lee, Young-Ran (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Giri, Sib Sankar (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Hyoun-Joong (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Yun, Sae-Kil (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Chi, Cheng (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Sang-Guen (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Koh, Jeong-Rack (Lotte World Aquarium) ;
  • Jung, Ji-Yun (Lotte World Aquarium) ;
  • Lee, Byeong-Chun (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Park, Se-Chang (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • 투고 : 2016.02.11
  • 심사 : 2016.04.04
  • 발행 : 2016.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Gradual mortality of look down fish (Selene vomer) was observed in a private aquarium in Seoul, showing abnormal swimming behavior and lethargy. A bacterial pathogen from kidney was cultured, identified, and confirmed as Vibrio harveyi using Vitek System 2 and 16S rRNA gene sequencing. A predominant bacterial strain, SNUVh-LW2 was proved to be most closely related to isolates from China by phylogenetic analysis with minimum evolution method. Also, tetracycline was considered as the most sensitive antibiotic agent via antibiotic susceptibility test. The group of fish was treated according to the diagnostic result and no more mortality was observed.

키워드

참고문헌

  1. Austin B, Zhang XH. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett Appl Microbiol 2006, 43, 119-124. https://doi.org/10.1111/j.1472-765X.2006.01989.x
  2. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966, 45, 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  3. Cano-Gomez A, Hoj L, Owens L, Andreakis N. Multilocus sequence analysis provides basis for fast and reliable identification of Vibrio harveyi-related species and reveals previous misidentification of important marine pathogens. Syst Appl Microbiol 2011, 34, 561-565. https://doi.org/10.1016/j.syapm.2011.09.001
  4. Cervigon F. Marine Fish of Venezuela. Vol. 2. Fundacion Cientifica Las Roques, Caracas, 1993.
  5. Clinical and Laboratory Standards Institute. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline. CLSI document M45-A. Clinical and Laboratory Standards Institute, Wayne, 2006.
  6. Filho JML, Lessa R, Stosic B, Neto PJD, Vieira JW. Morphological discrimination in juveniles of two Selene species (Teleostei: Carangidae) using truss net distances. Braz Arch Biol Technol 2006, 49, 231-238. https://doi.org/10.1590/S1516-89132006000300008
  7. Gomez DK, Lim DJ, Baeck GW, Youn HJ, Shin NS, Youn HY, Hwang CY, Park JH, Park SC. Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates. J Vet Sci 2006, 7, 369-374. https://doi.org/10.4142/jvs.2006.7.4.369
  8. Gomez-Gil B, Soto-Rodriguez S, Garcia-Gasca A, Roque A, Vazquez-Juarez R, Thompson FL, Swings J. Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms. Microbiology 2004, 150, 1769-1777. https://doi.org/10.1099/mic.0.26797-0
  9. Jacobina UP, Vicari MR, Martinez PA, Cioffi MB, Bertollo LAC, Molina WF. Atlantic moonfishes: independent pathways of karyotypic and morphological differentiation. Helgol Mar Res 2013, 67, 499-506. https://doi.org/10.1007/s10152-012-0338-8
  10. Menezes NA, Figueiredo JL. Marine Fish Manual of Southeastern Brazil. IV. Teleostei. Zoology Museum of University of Sao Paulo, Sao Paulo, 1980.
  11. Rico RM, Tapia-Paniagua S, Martinez-Manzanares E, Balebona MC, Morinigo MA. Characterization of Vibrio harveyi strains recovered from diseased farmed Senegalese sole (Solea senegalensis). J Appl Microbiol 2008, 105, 752-760. https://doi.org/10.1111/j.1365-2672.2008.03786.x
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013, 30, 2725-2729. https://doi.org/10.1093/molbev/mst197