• Journal of the korean society of oceanography
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• 제39권3호
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• pp.163-171
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• 2004

Sequences of the large subunit ribosomal (LSD) rDNA D1-D2 region of Alexandrium catenella(=A. sp. cf. catenella) and A. tamarense isolates, which were collected along the Korea coasts, were analyzed to understand their phylogenetic relationships and geographical distributions. All A. catenella and A. tamarense isolates belonged to the A. tamarense/catenella/fundyense complex and were grouped with the North American and temperate Asian ribotypes, respectively, regardless of the presence or absence of a ventral pore in the first apical plate. A consistent and peculiar characteristic that differentiated the Alexandrium isolates was amplification of a second PCR product with a lower molecular weight in addition to the predicted one; ten A. catenella isolates belonging to the temperate Asian ribotype yielded this additional PCR product. Sequence alignment revealed that the shorter PCR product resulted from an unusual large deletion of 87 bp in the LSD rDNA D1 domain. The North American and temperate Asian ribotypes were prevalent along the Korean coasts without geographical separation. Given the high genetic homogeneity among widely distributed Alexandrium populations, each ribotype appeared to be pandemic rather than to constitute a distinct regional population.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.71.2-71
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• 2003

Ralstonia solancearum infects many solanaceous plants, however race 3 infects only potato and tomato weakly. To identify genes responsible for race specificity of R. solanacearum, we mobilized genomic library of LSD2029 (race 3) into LSD341 (race 1) and inoculated 1,000 transconjugants into hot pepper. One transconjugant that did not induce wilt symptom in hot pepper was isolated. We found that a cosmid clone, pRSl, conferred avirulence to LSD341. By deletion and mutational analyses of pRSl, we found the 0.9-kb PstI/Hindlll fragment carries avirulence functions. We sequenced the fragment and identified one possible open reading frame, a rsal gene, possibly encoding 110 amino acids. The rsal was preceded with a plant-inducible promoter (PIP) box, indicating that the gene might be regulated by HrpB. Interestingly, the promoter region of the rsal homolog in the strain GM11000 (race 1) did not have the PIP box. Rsal did not show any significant homologies with proteins in the database, indicating th e protein is different from the previously reported avirulence proteins. When we mutated the rsal gene by marker-exchange in LSD2029, the mutant was less virulent in potato.

• 한국강구조학회 논문집
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• 제17권2호통권75호
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• pp.207-216
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• 2005

본 논문은 스테인리스 각형강관 및 원형강관에 대한 좌굴내력에 관한 연구로서 건축구조용 강재로서의 적용성 검토를 위해, 세장비를 주요 변수로 한 소재의 인장강도실험과 stub-column의 압축강도실험, 기둥의 중심압축실험을 실시하여 강재의 기계적 성질과 기둥의 강도 및 거동을 파악한다. 또한 이론해석과 각국 기준식(AIK-LSD, AISC-LRFD, AIJ-LSD, SIJ-ASD) 및 복수강도곡선의 적용을 통한 이론값과 실험값을 비교함으로서 건축구조용 강재로서 적용성 검토와 구조설계기준 확립을 위한 기초 자료를 구하는데 목적이 있다.

• 생물정신의학
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• 제3권2호
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• pp.245-250
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• 1996

본 연구는 알코올사용장애 환자의 혈소판막에 있는 serotonin수용체가 대조군과 비교했을 때 어떤 차이가 있는지 알아보기 위해 시행되었다. $_{125}I-LSD$를 radioligand로 사용하여 포화반응을 해 봄으로서 다음의 결과를 얻었다. 1) 환자군과 대조군간은 $B_{max}$에 있어서만 유의한 차이를 보였다(P<0.0017). 2) Type 1군과 type 2군 사이 역시 $B_{max}$에 있어서만 유의한 차이를 보였다(P<0.0396). 3) 알코올남용군과 알코올의존군 사이의 $B_{max}$와 $K_d$는 모두 유의한 차이가 없었다. 위 결과들은 알코올사용장애의 serotonin 결핍가설을 지지해주며 혈소판의 serotonin 수용체 수의 증가가 알코올사용장애의 trait marker가 될 수 있음을 시사해 준다.

• 융합신호처리학회논문지
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• 제13권4호
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• pp.173-179
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• 2012

본 논문에서는 자동차 번호판을 검출하는 새로운 방법을 제시한다. 자동차 번호판은 사각형 모양이므로 우리의 방법은 기본적으로 입력 영상에서 사각형을 추출하는 방법이 된다. 번호판을 검출하기 위해, 먼저 입력영상의 콘트라스트를 향상시키고, 그 후 LSD(Line segment detector) 기법을 사용하여 영상내의 선을 검출하고, 이 선 정보로 부터 사각형들을 추출 한다. 이 사각형들은 번호판 후보들이 되고, 이로부터 번호판이 검출된다. 이중에서 본 연구가 제안하는 부분은 사각형 추출방법으로서, 이 방법은 3단계로 구성된다: (1) 먼저, LSD에 의해 얻어진 선으로부터 꼭짓점들을 추출한다; (2) 구해진 꼭짓점들을 사용하여 사각형의 대각선을 검출한다; (3) 그 후, 대각선 정보를 이용하여 사각형을 추출해 낸다. 최종적으로 번호판 특성과 사각형 내부 정보를 이용하여 이 사각형들로부터 번호판이 선택된다. 100장의 자동차 영상을 촬영하여 실험한 결과 94%의 검출율을 달성하였다.

• 한국동물위생학회지
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• 제45권2호
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• pp.133-137
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• 2022

Lumpy skin disease (LSD) is a severe cross-border infectious disease that causes fever, skin and visceral nodules in cattle. LSD is caused by the lumpy skin disease virus (LSDV), a dsDNA virus that belongs to the genus Capripoxvirus. Although LSD has been found only in Southern Africa traditionally, in the last decade it is spreading very quickly through the Middle East and into Eastern Europe and China. It usually affects cattle and water buffalos being transmitted by blood-feeding insects. As it causes a huge economic impact, LSD is a notifiable disease by World Organisation for Animal Health, and managed as the legal infectious disease class I in Korea. Therefore, the purpose of this study was to confirm the existence of LSDV antigens or antibodies in Korean livestock. We collected 1,200 blood samples from cattle (Korean native and dairy cattle) and Korean water deer in 4 major provinces of the country, then tested the existence of LSDV antigen and antibody. None (0.0%) of the 1,200 blood samples were positive for both antigen and antibody of LSDV. To the best of our knowledge, this is the first study that examines the prevalence of LSDV in Korea. Our study aims to report the LSDV occurrence situation obtained by surveillance in Korea and provide information that may help prevention of LSD epidemics.

• BMB Reports
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• 제56권12호
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• pp.657-662
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• 2023

Neurodegenerative diseases are characterized by distinct protein aggregates, such as those of α-synuclein and tau. Lysosomal defect is a key contributor to the accumulation and propagation of aberrant protein aggregates in these diseases. The discoveries of common proteinopathies in multiple forms of lysosomal storage diseases (LSDs) and the identification of some LSD genes as susceptible genes for those proteinopathies suggest causative links between LSDs and the proteinopathies. The present study hypothesized that defects in lysosomal genes will differentially affect the propagation of α-synuclein and tau proteins, thereby determining the progression of a specific proteinopathy. We established an imaging-based high-contents screening (HCS) system in Caenorhabditis elegans (C. elegans) model, by which the propagation of α-synuclein or tau is measured by fluorescence intensity. Using this system, we performed RNA interference (RNAi) screening to induce a wide range of lysosomal malfunction through knock down of 79 LSD genes, and to obtain the candidate genes with significant change in protein propagation. While some LSD genes commonly affected both α-synuclein and tau propagation, our study identified the distinct sets of LSD genes that differentially regulate the propagation of either α-synuclein or tau. The specificity and efficacy of these LSD genes were retained in the disease-related phenotypes, such as pharyngeal pumping behavior and life span. This study suggests that distinct lysosomal genes differentially regulate the propagation of α-synuclein and tau, and offer a steppingstone to understanding disease specificity.

• Asia pacific journal of information systems
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• 제6권1호
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• pp.241-267
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• 1996

The problems like exceeding estimated cost, late due-date, expensive maintenance, insuffiency of experts and low productivity are becoming emphasized related with the software development. In order to overcome these problems and to develop the highly qualified software within the limited resources, a project management tool is used. The purpose of this study is to develop a conceptual model of the project management system which can plan, analyze and control the software development projects effectively. The model is constructed with plan and track management system, cost management system and operation management system for the efficient project management. It is named by LSD-PMS : Large-scale Software Development Project Management System. We reviewed 5 cases of project management. LSD-PMS is evaluated and reviewed by the project managers in the field. It is proved that LSD-PMS is a tool which can help project managers develop software successfully given budget and time schedule. In the future, this system should be further developed as an integrated model with system implementation tools such as CASE products.

• 대한안전경영과학회지
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• 제12권2호
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• pp.217-223
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• 2010

The research proposes the complementary methodology using integrated hypothesis testing and confidence interval models that can be identified the statistical difference and practical equivalence. The models developed in this study can be used in the quality improvement processes such as QC story 15 steps. For the expressions of CI4LSD(Confidence Interval for Least Significant Difference) and CI4TOST(Confidence Interval for Two One-Sided Tests) are simple, quality practioners can efficiently handle them. CI4TOST models as a complement can be applied when CI4LSD models are influenced by sample size and precision.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.884-886
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• 2001

The reactivity and stability of purified Lignostilbene- ${\alpha}$,${\beta$}-dioxygenase (LSD)-I were examined. Its optimum temperature was $50^{\circ}C$ at pH 8.5, but it was stable only up to $30^{\circ}C$. The activity of LSD-I increased 12-fold by $30\%$, with increased $V_{max}$ and lowered $K_m.$ LSD-I was stable in 10% methanol.