Browse > Article

A Molecular Phylogenetic Study on Korean Alexandrium catenella and A. tamarense Isolates (Dinophyceae) Based on the Partial LSD rDNA Sequence Data  

Kim, Keun-Yong (Department of Aquaculture, Pukyong National University)
Kim, Chang-Hoon (Department of Aquaculture, Pukyong National University)
Publication Information
Journal of the korean society of oceanography / v.39, no.3, 2004 , pp. 163-171 More about this Journal
Abstract
Sequences of the large subunit ribosomal (LSD) rDNA D1-D2 region of Alexandrium catenella(=A. sp. cf. catenella) and A. tamarense isolates, which were collected along the Korea coasts, were analyzed to understand their phylogenetic relationships and geographical distributions. All A. catenella and A. tamarense isolates belonged to the A. tamarense/catenella/fundyense complex and were grouped with the North American and temperate Asian ribotypes, respectively, regardless of the presence or absence of a ventral pore in the first apical plate. A consistent and peculiar characteristic that differentiated the Alexandrium isolates was amplification of a second PCR product with a lower molecular weight in addition to the predicted one; ten A. catenella isolates belonging to the temperate Asian ribotype yielded this additional PCR product. Sequence alignment revealed that the shorter PCR product resulted from an unusual large deletion of 87 bp in the LSD rDNA D1 domain. The North American and temperate Asian ribotypes were prevalent along the Korean coasts without geographical separation. Given the high genetic homogeneity among widely distributed Alexandrium populations, each ribotype appeared to be pandemic rather than to constitute a distinct regional population.
Keywords
Alexandrium catenella; Alexandrium tamarense; geographical distribution; LSD rDNA; molecular phylogeny;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cavalier-Smith, T, 1993. Kingdom Protozoa and its 18 phyla. MicrobioI. Rev. 57: 953-994
2 Cembella, A.D., F.J.R. Taylor and J.C. Therriault, 1988. Cladistic analysis of electrophoretic variants within the toxic dinoflagellate genus Protogonyaulax.Bot. Mar., 31: 39-51
3 Daugbjerg, N., G. Hansen, J. Larsen and $\phi$. Moestrup, 2000. Phy-logeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data,including the erection of three new genera of unarmored dinoflaeellates. Phycotogia, 39: 302-317
4 Delgado, M., E. Garces, M. Vila and J. CamP, 1997. Morphologi-cal variability in three populations of the dinoflagellate Atex andrium taylori. J. Ptankton Res.. 19: 749-757
5 Saldarriaga, J.F., F.J.R. Taylor, P.J. Keeling and T. Cavalier-Smith, 2001. Dinoflagellate nuclear SSU rRNA phylogeny suggests multiple plastid losses and replacements. J. Mot. Evol., 53: 204-213
6 Saunders, G.W., D.R.A. Hill, J.P. Sexton and R.A. Andersen, 1997. Small-subunit ribosomal RNA sequences from selected dinoflagellates: testing classical evolutionary hypothesis with molecular systematic methods, In: Origins of Algae and their Plastids, edited by Bhattacharya, D., Springer, New York, pp. 237-259
7 Scholin, C.A., M. Herzog, M. Sogin and D.M. Anderson, 1994. Identification of group- and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. J. Phycol., 30: 999-1011
8 Uribe, P.C., B.A. Su$\rez-Isla and R.T. Espejo, 1999. Ribosomal RNA heterogeneity and identification of toxic dinoflagellate cultures by heteroduplex mobility assay. J. Phycol., 35: 884-888
9 van Oppen, M.J.H., J.L. Olsen, W.T. Stam, C. van den Hoek and C. Wiencke, 1993. Arctic-antarctic disjunctions in the benthic seaweeds Acrosiphonia arcta (Chlorophyta) and Desmarestia viridis/willii (Phaeophyta) are of recent origin. Mar. Biol., 115: 381-386
10 Yeung, P.K.K., K.F. Kong, F.T.W. Wong and J.T.Y. Wong, 1996. Sequence data for two large-subunit rRNA genes from an Asian strain of AIexandrium catenella. Appl. Environ. Microbiol., 62: 4199-4201
11 Yentsch, C.M., B. Dale and J.W. Hurst, 1978. Coexistence of toxic and non-toxic dinoflagellates resembling Gonyaulax tamarensis in New England coastal waters. J. Phycol., 14: 330-332
12 Balech, E., 1995. The Genus Alexandrium Halim (Dinflagellata). Sherkin Island Marine Station, Co. Cork, 151 pp
13 Cembella, A.D., J.J. Sulli'van, G.L. Boyer, F.J.R. Taylor and R.J.Andersen, 1987. Variation in paralytic shellfish toxin compo- sition within the Protoeonyaulax tamarensis/catenetIa spe- cies complex: red tide dinoflagellates. Biochem. Syst. EcoL, 15:171-186   DOI   ScienceOn
14 Thompson, J.D., D.G. Higgins and T.J. Gibson, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucteic Acids Res., 22: 4673-4680
15 Zardoya, R., E. Costas, V. L$\pez-Rodas, A. Garriod-Pertierra and J.M. Bautista, 1995. Revised dinoflagellate phytogeny inferred from molecular analysis of large-subunit ribosomal RNA gene sequences. J. Mol. Evol., 41: 637-645
16 Medlin, L.K., M. Lange, U. Wellbrock, G. Donner, M. Elbr$\"{a}$hter, C. Hummert and B. Luckas, 1998. Sequence comparisons link toxic European isolates of Atexandrium tamarense from the Orkney isolates to toxic North American stocks. Eur. J. Protistol, 34: 329-335
17 Posada, D. and K.A. Crandall, 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics, 14: 817-818
18 Rehnstam-Holm, A.-S., A. Godhe and D.M. Anderson, 2002. Molecular studies of Dinophysis (Dinophyceae) species from Sweden and North America. Phycologia, 41: 348-357
19 Scholin, C.A. and D.M. Anderson, 1996. LSU rDNA-based RFLP assays for discriminating species and strains of Alexandrium (Dinophyceae). J. Phycol., 32: 1022-1035
20 Scholin, C.A., D.M. Anderson and M.L. Sogin, 1993. The existence of two distinct small-subunit rRNA genes in the North American toxic dinoflagellate Alexandrium fundyense (Dinophyceae). J. Phycol., 29: 209-216
21 de Vargas, C., R. Norris, L. Zaninetd, S.W. Gibb and J. Pawlowski, 1999. Molecular evidence of cryptic speciation in planktonic fora-minifers and their relation to oceanic province. Proc. Natt. Acad. Sci. USA, 96: 2864-2868
22 Higman, W.A., D.M. Stone and J.M. Lewis, 2001. Sequence com-parisons of toxic an non-toxic Atexandrium tamarense (Dino phyceae) isolates from UK waters. Phycotogia, 40: 256-262
23 Scholin, C.A., G.M. Hallegraeff and D.M. Anderson, 1995. Molecular evolution of the Alexandrium tamarense 'species complex' (Dinophyceae): dispersal in the North American and West Pacific regions. Phycotogia, 34: 472-485
24 Lee, S.Y., H.W. Hee, J.G. Park, J.A. Lee and Y.S. Park, 1998. Molecular phylogeny of phytoplankton isolated from red tides in southern coast of Korea. Bada. 3: 90-93 (in Korean)
25 Mylvaganam, S. and P.P. Dennis, 1992. Sequence heterogeneity between the two genes encoding 16S rRNA from the halophilic archaebacterium Haloarcula marismortui. Genetics, 130: 399-410
26 Olsen, G.J., C.R. Woese and R. Overberbeek, 1994. The winds of (evolutionary) change: Breathing new life into microbiology. J. Bacteriol., 176: 1-6
27 Felsenstein, J., 1985. Confidence limits on phylogenies: an approachusing the bootstrap. Evotution, 38: 16-24
28 Hansen, G., N. Daugbjerg and J.M. Franco, 2003. Morphology, toxin composition and LSU rDNA phytogeny of Atexandriumminutum (Dinophyceae) from Denmark, with some morpho-logical observations on other European strains. HarmfutAtgae, 2: 317-335
29 Swofford, D.L., 2001. 'PAU$P^{*}$: Phylogenetic Analysis Using Parsimony (* and Other Methods), Version 4.0b8', Sinauer, Sunderland
30 Hallegraeff, G.M., 1993. A review of harmful algal blooms andtheir apparent global increase. Phycotogia, 32: 79-99
31 N$\"{u}$bel, U., B. Engelen, A. Felske, J. Snaider, A. Wieshuber, R.I. Amann, W. Ludwig and H. Backhaus, 1996. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J. Bacteriol, 178: 5636-5643
32 Gunderson, J.H., M.L. Sogin, G. Wollett, M. Hollingdale, V.F. de la Cruz and T.F. McCutchan, 1987. Structurally distinct, stage-specific ribosomes occur in PIasmodium. Science, 238: 933-937
33 Cembella, A.D. and F.J.R. Taylor, 1986. Electrophoretic variabil-ity within the Protogonyautax tamarensis/cateneIta species complex: pyridine linked dehydrogenases. Biochem. Syst. Ecot., 14:311-323
34 Cho, C.H., 1978. On the Gonyautax red tide in Jinhae Bay. Bull. Kor. Fish. Soc., 11: 111-114 (in Korean)
35 Chang, D.S., I.S. Shin, J.H. Pyeun and Y.H. Park, 1987. A study on paralytic shellfish poison of sea mussel, MytiIus edutis,Korea. Butt. Kor. Fish. Soc. 20: 293-299 (in Korean)
36 Hong, Y.-K., S.-D. Kim, M. Polne-Fuller and A. Gibor, 1995.DNA extraction conditions from Porphyra perforata usingLiCl. J. Appt. PhycoI., 7: 101-107
37 Kim, K.-Y., M. Yoshida, Y. Fukuyo and C.-H. Kim, 2002. Mor-phological observation of AIexandrium tamarense (Lebour)Balech, A. catenetta (Whedon and Kofoid) Balech and relatedone morphotype (Dinophyceae) in Korea. Atgae, 17: 11-19
38 Fukuyo, Y., 1985. Morphology of Protogonyautax tamarensis(Lebour) Taylor and Protogonyaulax catenella (Whedon andKofoid) Taylor from Japanese coastal waters. Butt. Mar. Sci., 37:529-537
39 Hayhome, B.A., D.M. Anderson, D.M. Kulis and D.J. Whitten, 1989. Variation among congeneric dinoflagellates from the northeastern United States and Canada. I. Enzyme electro-phoresis. Mar. BioL, 101: 427-435
40 Darling, K.R, C.M. Wade, I.A. Stewart, D. Kroon, R. Dingle andA.J.L. Brown, 2000. Molecular evidence for genetic mixingof Arctic and Antarctic subpolar populations of planktonic foraminifers. Nature, 405: 43-47   DOI   ScienceOn
41 Lee, J.S., I.S. Shin, Y.M. Kim and D.S. Chang, 1997. Paralyticshellfish toxins in the mussel, MytiIus edutis, caused theshellfish poisoning accident at Geoje, Korea, in 1996. J. Kor.Fish. Soc., 30: 158-160 (in Korean)
42 Adachi, M., Y. Sako, Y., Ishida, D.M. Anderson and B. Reguera, 1993. Cross-reactivity of five monoclonal antibodies to vari- ous isolates of Atexandrium as determined by an indirectimmunofluorescence method. Nippon Suisan Gakkaishi, 59: 1807