• Title/Summary/Keyword: LS-1

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Preconditioning for Cryopreservation of in vitro Grown Bulblets of Lily using Droplet-Vitrification

  • Jae-young Song;Jinjoo Bae;Young-yi Lee;Jung-yoon Yi;Jung-ro Lee;Mun-sup Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.35-35
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    • 2021
  • This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

A Study of Reliability and Validity of the Learning Satisfaction and Learning Flow Scale in Korean Medical Ophthalmology & Otolaryngology and Dermatology (안이비인후과목, 피부외과목의 전공만족 및 전공 몰입경험 척도의 신뢰도와 타당도 연구)

  • Kim, Chul-Yun;Kim, Nam-Kwen;Kwon, Kang;Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.28 no.2
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    • pp.54-65
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    • 2015
  • Objective : This Study was conducted in order to develop the Learning Satisfaction (LS)and Learning Flow Scale(LFS) for Korean Medical Ophthalmology & Otolaryngology & Dermatology and to investigate the reliability and validity. Methods : LF and LFS were administered to 44 students and 20 resident physicians . We examined the test-retest reliability, internal consistency and perfomed factor analysis. Result : 1. Test-retest reliability were reliable on LS and LFS for Korean Medical Ophathalmology & Otolaryngology.2. Test-retest reliability were unreliable comparatively on LS and LFS for Korean Medical Dermatology.3. internal consistency were all reliable in LS and LFS Korean Medical Ophthalmology & Otolaryngology & Dermatology.4. factor analysis were reliable only LS of Korean Medical Dermatology. Conclusions : LS and LFS has reliability to measure learning satisfaction and learning flow of Korean Medical students. But there is no evidence about validity of LS and LFS for Korean Medical Ophthalmology & Otolaryngology & Dermatology.

Expression pattern of low-temperature-related genes by the treatment of AFP in the cryopreservation of potato shoot tips (감자 정아 동결보존과정에서 AFP 처리 시 저온관련 유전자의 발현양상)

  • Seo, Ji-Hyang;Jeon, Su Min;Naing, Aung Htay;Han, Jeung Sul;Park, In Hwan;Kim, Chang-Kil
    • Journal of Plant Biotechnology
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    • v.44 no.4
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    • pp.478-483
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    • 2017
  • The expression profiles of low-temperature-related genes were examined in in vitro potatoes exposed to a cold condition for 1-3 days. The expression levels of PPI1 and CI21B genes were lineraly elevated from day 1 to day 3, while the opposite trend was observed for CBF4 and CI21A. In addition, the expression of the genes CI21A and CI21B varied, along with specific tissues (leaf, stem, and tuber) and the treatment periods. Therefore, potato shoot tips were cryopreserved with LS and PVS2 containing different oncentrations of AFP. It can thus be inferred that the presence of AFP in LS and PVS2 was likely to elevate expression pattern of the genes. Furthermore, the concentration of AFP (1,500 ng/ml for LS and 500 ng/mg for PVS2) was the best for the cryopreservation of potatoes.

THE DEVELOPMENT OF POWER SYSTEM ANALYSIS ONLINE PROGRAMS FOR THE KOREAN EMS (한국형 EMS용 온라인 전력계통해석 프로그램 개발)

  • Lee, Uk-Hwa;Cho, Y.S.;Yun, S.Y.;Lee, J.;Hur, S.I.;Kim, S.G.;Lee, H.S.
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.9-10
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    • 2008
  • 전력IT 국책과제로 진행되는 한국형 EMS용 계통해석 프로그램 개발의 1단계 성과로는 온라인 토폴로지 프로세서, 상태추정, 휴전계획 등이며 스터디로 급전원 조류계산 등이다. 2년간에 걸친 개발 과정을 거치고 지난 08년1월${\sim}$08년4월까지 목천 한국전력거래소 후비센타에서 온라인 계통 데이터을 이용하여 다양한 실증시험, 특히 한국전력거래소의 급전원 입회시험까지 완료된 상태이다. 1단계로 개발된 프로그램 중 토폴로지, 상태추정, 휴전계획 등 프로그램 및 실증시험 결과를 중심으로 기술하고 있다.

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1.31 um Uncooled DFB-LD with High Slope Efficiency for G-PON Application (G-PON용 높은 전광변환효율을 갖는 1.31 um 비냉각 DFB-LD)

  • Kim, Jeong-Ho;Pi, Joong-Ho;Kim, Deok-Hyun;Park, Chil-Sung;Ryu, Han-Gwon;Koo, Bon-Jo
    • Korean Journal of Optics and Photonics
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    • v.18 no.5
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    • pp.333-336
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    • 2007
  • A Strained Layer Multiquantum-Well (SL-MQW) distributed feedback laser at a wavelength of 1.31 um operating from $-40^{\circ}C$ to $85^{\circ}C$ without any cooling is grown by metal-organic chemical vapor deposition (MOCVD). Lasers with high slope efficiency are achieved through careful optimization of a SL-MQW active layer, especiallyoptimizing the amount of strain, the well thickness, the barrier thickness, the number of wells, and the active layer width. In this paper, we obtain the slope efficiencies of 0.38[mW/mA] and 0.26 [mW/mA] at $25^{\circ}C$ and $85^{\circ}C$, respectively. Threshold currents are 7.1[mA] and 19.8[mA] at $25^{\circ}C$ and $85^{\circ}C$, respectively.

Effects of Low Dose $\gamma$ Radiation on Callus Growth of Lithospermum erythrorhizon S. (지치 (Lithospermum erythrorhizon S.) Callus 생장에 미치는 저선량 $\gamma$선의 효과)

  • 황혜연;김재성;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.305-310
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    • 2001
  • The effect of low dose ${\gamma}$-radiation on the callus growth of Lithospermum erythrorhizon S. cultured on different medium and lighting condition was investigated. The 8 Gy irradiation stimulated callus growth on LS medium supplemented with BA 2 mg/L and NAA 2 mg/L, however, the growth of callus was more effective on LS medium supplemented with BA 1 mg/L and NAA 1 mg/L under 16 hrs day light. And on the LS medium containing IAA 0.2 mg/L, 16 Gy irradiation increased the callus growth by supplement with kinetin 2 mg/1 and the effect of kinetin was higher than BA at same concentration. The growth of callus was more vigorous on LS medium than MS medium in general. On M-9 medium, the growth of callus was poor regardless lighting conditions, however, by ${\gamma}$-ray irradiation of 16 Gy or 30 Gy, callus growth rates were increased by 30% or more than 30%, especially, under 16 hrs day light condition.

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1~9월 일본 카메라 출하 - 디지털 카메라 2배 이상 성장 SLR, LS국내 출하$\cdot$수출 감소

  • 한국광학기기협회
    • The Optical Journal
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    • v.12 no.6 s.70
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    • pp.53-55
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    • 2000
  • 디지털카메라가 더욱 강세를 보이고 있으며 이 추세는 내년까지 계속 이어질 것으로 예상된다. 상대적으로 SLR, LS카메라는 점점 감소하고 있다. 올 1월부터 9월까지 일본 카메라 전체 생산 대수는 2,085만 3천대로 전년 같은 기간에 비해 약간 성장했으나, 국내 출하와 수출은 모두 감소했다. 특히 일본 국내 출하 감소가 두드러져 SLR, LS 각각 전년 동기 대비 83.9$\%$, 85.2$\%$에 그쳤다. 반면

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Validation of Analytical Methods for Unriped Rubus crataegifolius, Gardenia jasminoides and Ulmus macrocarpa Marker Compounds for Standardization of Natural Complex LS-RUG-com Preparation as Functional Ingredient (천연복합소재 LS-RUG-com의 기능성원료 표준화를 위한 산딸기 미숙과, 치자 및 유백피 지표성분의 분석법 검증)

  • Young Ik Lee;Su Jin Pyo;Hee Jin Lee;Hye Jung Yoon;Ho Yong Sohn;Jin Sook Cho
    • Journal of Life Science
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    • v.34 no.1
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    • pp.59-67
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    • 2024
  • LS-RUG-com preparation in a complex extract from mixture of three natural plants. Rubus crataegifolius/unriped, Gardenia jasminoides and Ulmus marcrocarpa that have been widely used in traditional functional health food. This study was conducted to establish the HPLC analysis methods that can be used to establish quantitative analysis of R. crataegifolius, G. jasminoides and U. macrocarpa for standardization of LS-RUG-com preparations. HPLC analysis methods for simultaneous determination of ellagic acid and geniposide and single determination of catechin-7-O-β-D-apiofuranoside were established for the quality control of natural plants complex (LS-RUG-com). Validation of HPLC analysis were performed by checking specificity, accuracy, precision, limit of detection and quantitation, and linearity following ICH (International Council for Harmonisation) guideline. As the result of quantitative analysis, the contents of ellagic acid, geniposide and catechin-7-O-β-D-apiofuranoside in each plant extracts were 11.2 mg/g (ellagic acid) and 72 mg/g (geniposide) and 10.2 mg/g (catechin-7-O-β-D-apiofuranoside). The contents of ellagic acid, geniposide and catechin-7-O-β-D-apiofuranoside in LS-RUG-com were 4.62~6.82 mg/g (ellagic acid), 19.2~28.8 mg/g (geniposide) and 1.36~2.04 mg/g (catechin-7-O-β-D-apiofuranoside) respectively.

Segmental Liver Stiffness Evaluated with Magnetic Resonance Elastography Is Responsive to Endovascular Intervention in Patients with Budd-Chiari Syndrome

  • Peng Xu;Lulu Lyu;HaitaoGe;Muhammad Umair Sami;Panpan Liu;Chunfeng Hu;Kai Xu
    • Korean Journal of Radiology
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    • v.20 no.5
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    • pp.773-780
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    • 2019
  • Objective: To assess segmental liver stiffness (LS) with MRI before and after endovascular intervention in patients with Budd-Chiari syndrome (BCS). Materials and Methods: Twenty-three patients (13 males and 10 females; mean age, 42.6 ± 12.6 years; age range, 31-56 years) with BCS as a primary liver disease were recruited for this study. Two consecutive magnetic resonance elastography (MRE) examinations were performed before the endovascular treatment. Fifteen patients who underwent endovascular intervention treatment also had follow-up MRE scans within three days after the procedure. LS was measured in three liver segments: the right posterior, right anterior, and left medial segments. Inter-reader and inter-exam repeatability were analyzed with intraclass correlation coefficients (ICCs) and Bland-Altman analysis. Segmental LS and clinical characteristics before and after the intervention were also compared. Results: Within three days of the endovascular intervention, all three segmental LS values decreased: LS of the right posterior segment = 7.23 ± 0.88 kPa (before) vs. 4.94 ± 0.84 kPa (after), LS of the right anterior segment = 7.30 ± 1.06 kPa (before) vs. 4.77 ± 0.85 kPa (after), and LS of the left medial segment = 7.22 ± 0.87 kPa (before) vs. 4.87 ± 0.72 kPa (after) (all p = 0.001). There was a significant correlation between LS changes and venous pressure gradient changes before and after treatments (r = 0.651, p = 0.009). The clinical manifestations of all 15 patients significantly improved after therapy. The MRE repeatability was excellent, with insignificant variations (inter-reader, ICC = 0.839-0.943: inter-examination, ICC = 0.765-0.869). Bland-Altman analysis confirmed excellent agreement (limits of agreement, 13.4-19.4%). Conclusion: Segmental LS measured by MRE is a promising repeatable quantitative biomarker for monitoring the treatment response to minimally invasive endovascular intervention in patients with BCS.

Electrical Properties on Kraft Insulating Paper used as HVDC Cable (HVDC용 크라프트 절연지의 전기적 특성 평가)

  • Youn, B.H.;Lee, S.J.;Kim, J.N.;Kim, D.W.;Jeon, S.I.
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.2250-2252
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    • 2005
  • 본 논문은 HVDC용 크라프트 절연지의 기본 전기적 특성평가를 목적으로 온도별 역률의 변화, 절연저항 및 임펄스 절연파괴강도 등을 측정한 결과이다. 온도에 따른 크라프트 절연지의 역률은 $50{\sim}70^{\circ}C$에서 최소 값을 나타내었으며, 밀도가 낮은 시료일수록 낮은 역률을 나타내었다. 하지만, 임펄스 절연파괴 강도는 밀도가 낮을수록 저하되는 특성을 나타내어 적절한 밀도의 선정이 필요하다는 사실을 알았다. 또한, 온도 및 인가 전계에 따른 절연저항을 측정하여 크라프트 절연지의 온도상수 및 전계상수를 측정한 결과, 온도상수 ${\alpha}$$0.09{\sim}0.1^{\circ}C^{-1}$, 전계상수는 약 1.5을 얻었다. 이는 HVDC용 케이블의 설계 시 절연체의 전계분포 및 두께 산정에 기본적인 데이터를 제공할 수 있다.

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