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http://dx.doi.org/10.5010/JPB.2017.44.4.478

Expression pattern of low-temperature-related genes by the treatment of AFP in the cryopreservation of potato shoot tips  

Seo, Ji-Hyang (Department of Horticulture, Kyungpook National University)
Jeon, Su Min (Department of Horticulture, Kyungpook National University)
Naing, Aung Htay (Department of Horticulture, Kyungpook National University)
Han, Jeung Sul (Department of Horticulture, Kyungpook National University)
Park, In Hwan (Department of Landscape Architecture, Kyungpook National University)
Kim, Chang-Kil (Department of Horticulture, Kyungpook National University)
Publication Information
Journal of Plant Biotechnology / v.44, no.4, 2017 , pp. 478-483 More about this Journal
Abstract
The expression profiles of low-temperature-related genes were examined in in vitro potatoes exposed to a cold condition for 1-3 days. The expression levels of PPI1 and CI21B genes were lineraly elevated from day 1 to day 3, while the opposite trend was observed for CBF4 and CI21A. In addition, the expression of the genes CI21A and CI21B varied, along with specific tissues (leaf, stem, and tuber) and the treatment periods. Therefore, potato shoot tips were cryopreserved with LS and PVS2 containing different oncentrations of AFP. It can thus be inferred that the presence of AFP in LS and PVS2 was likely to elevate expression pattern of the genes. Furthermore, the concentration of AFP (1,500 ng/ml for LS and 500 ng/mg for PVS2) was the best for the cryopreservation of potatoes.
Keywords
Real-time PCR; Vitrification; LS; PVS2;
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Times Cited By KSCI : 2  (Citation Analysis)
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