• Title/Summary/Keyword: LS-1

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Quality Characteristics and Antioxidant Activity of Doenjang Made from Lipoxygenase-Free Genotypes Soybeans (Lipoxygenase 결핍콩으로 제조한 된장의 품질 특성 및 항산화 활성)

  • Lee, Soo-Jung;Kim, In-Sung;Hu, Wen-Si;Ha, Eun-Seon;Chung, Jong-Il;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.1
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    • pp.35-43
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    • 2016
  • To evaluate processing suitability of lipoxygenase (LOX)-free genotype soybeans (Jinyang, 05C4 and LS), quality characteristics and antioxidant activities of their Doenjang products were compared to those of Doenjang made from Taekwang soybean (LOX-present) as the control. Moisture and crude protein contents of Doenjang were significantly higher in LS than the control. Crude lipid content was not significantly different and was in the range of 7.92~8.22% in all samples. Carbohydrate content was significantly lower in LS than Taekwang. Content of reducing sugar in Doenjang was significantly higher in Jinyang than Taekwang. Contents of amino-type nitrogen were significantly higher in LOX-free cultivars than Taekwang. Colors of 05C4 and LS Doenjang were not different compared to that of Taekwang, whereas Jinyang Doenjang showed a noticeable color difference. Taekwang Doenjang had a slightly stronger savory taste than another sample. LOX-free cultivars had a strong salty taste. Flavor was the strongest in LS, whereas overall acceptability showed little difference among all samples. The total amino acid content was slightly higher in Jinyang and 05C4 than Taekwang, and content of essential amino acids was higher in Doenjang from LOX-free cultivars than Taekwang. Isoflavon content was significantly higher in Doenjang ($522.16{\sim}684.46{\mu}g/g$) made from LOX-free cultivars than in Taekwang ($374.79{\mu}g/g$). Total phenol content was significantly higher in 05C4 and LS, and flavonoid content was significantly higher in LS than Taekwang. Antioxidant activities were highest in Jinyang based on DPPH radical scavenging and reducing power. ABTS radical scavenging activity was significantly higher in Doenjang made from LOX-free cultivars than Taekwang. These results suggest that Doenjang from LOX-free cultivars could have a suitable genotype for Doenjang processing since it is more effective in terms of amino acids, isoflavone contents, and antioxidant activity.

Effect of Ledebouriella seseloides Extracts on Lipid Parameters in Ovariectomized Rats (방풍나물 추출물이 난소절제 흰쥐의 혈중 지질 변화에 미치는 영향)

  • Jeon, Myeong-Jeong;Kim, Mihyang
    • Journal of Life Science
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    • v.26 no.1
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    • pp.83-90
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    • 2016
  • This study was investigated the improvement effects of Ledebouriella seseloides (LS) ethanol extracts on lipid parameters in an ovariectomized animal model. Sixty, nine-week old female Sprague Dawley rats were randomly assigned to four groups as follows: sham-operated rats (SHAM), ovariectomized rats (OVX-CON) and ovariectomized rats that were treated with LS ethanol extracts (50 mg/kg/day and 200 mg/kg/day, respectively). The diets were fed to the rats for six weeks after their operation. The total-cholesterol and triglyceride contents on serum increased in the OVX-CON group compared to the SHAM group, but supplementation with the LS extract caused these factors to decrease. Notably, the serum LDL-cholesterol concentration in the supplemented 200 mg/kg/day LS ethanol extract group was significantly more reduced than the OVX-CON group. In addition, the platelet aggregation ability was lower in groups treated with LS than in the OVX-CON group. The alkaline phosphatase (ALP) activity was lower in the LS extract group compared to the OVX-CON group. Collagen content, in bone and cartilage, were reduced by ovariectomy, but the supplemented LS extract groups exhibited higher concentrations in their bones. According to these results, the improvement effects of LS extract on serum lipid parameters and osteogenesis in ovariectomized rats were illuminated.

Characterization of Mutant Strain, Lactobacillus paracasei ML-7 Isolated from Kimchi, and Its Effect on the Growth of Broiler (김치 유래의 유산균 변이주 Lactobacillus paracasei ML-7의 특성 및 육계 성장에 대한 영향)

  • Park, Keun-Tae;Oh, Mihyang;Nam, Jungok;Ji, Kibbeum;Han, Jong-Kwon
    • Korean Journal of Food Science and Technology
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    • v.46 no.2
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    • pp.148-152
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    • 2014
  • A mutant strain of Lactobacillus paracasei (ML-7) was derived by ultraviolet irradiation of the parent L. paracasei LS-2 strain, followed by colony selection on pH 3.5 MRS agar plates, on which L. paracasei LS-2 cannot grow. The L. paracasei ML-7 mutant strain produced an increased amount of organic acids compared to the L. paracasei LS-2 parent strain. Broiler chickens were randomly fed with one of three experimental diets: a basal diet as a control, and basal diets supplemented with 0.3% of the L. paracasei LS-2 and L. paracasei ML-7 strains. When concluding the study, the final body weights of the broilers fed with the supplemented basal diets were found to be significantly higher (1.9% for LS-2 and 6.2% for ML-7) than the control broiler group. Broilers fed with basal diet supplemented with the mutant L. paracasei ML-7 strain showed increased body weight and food intake, compared to broilers fed with the basal diet with or without the parent L. paracasei LS-2 strain.

The influence of shade allocation or total shade plus overhead fan on growth performance, efficiency of dietary energy utilization, and carcass characteristics of feedlot cattle under tropical ambient conditions

  • Castro-Perez, Beatriz I.;Estrada-Angulo, Alfredo;Rios-Rincon, Francisco G.;Nunez-Benitez, Victor H.;Rivera-Mendez, Carlos R.;Urias-Estrada, Jesus D.;Zinn, Richard A.;Barreras, Alberto;Plascencia, Alejandro
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.6
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    • pp.1034-1041
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    • 2020
  • Objective: The objective of this experiment was to evaluate the effect of shade allocation and shade plus fan on growth performance, dietary energy utilization and carcass characteristics of feedlot cattle under tropical ambient conditions Methods: Two trials were conducted, involving a total of 1,560 young bulls (289±22 kg BW) assigned to 24 pens (65 bulls/pen and 6 pens/treatment). Pens were 585 ㎡ with 15 m fence line feed bunks. Shade treatments (㎡ shade/animal) were: i) limited shade (LS) to 1.2 ㎡ shade/animal (LS1.2); ii) limited shade to 2.4 ㎡ shade/animal (LS2.4); iii) total shade (TS) which correspond to 9 ㎡/animal, and iv) total shade equipped with fans (TS+F). Trials lasted 158 and 183 days. In both studies, the average weekly maximum temperature exceeded 34℃. Results: Increasing shade allocation tended (p = 0.08) to linearly increases average daily gain (ADG), and dry matter intake (DMI, quadratic effect, p = 0.03). This effect was most apparent between LS1.2 and LS2.4. Shade allocation, per se, did not affect gain efficiency or estimated dietary net energy (NE). Compared with TS, TS+F increased (p<0.05) ADG, gain efficiency, and tended (p = 0.06) to increase dietary NE. There was a quadratic effect of shade on longissimus area and marbling score, with values being lower (p<0.01) for LS2.4 than for LS1.2 or TS. Likewise, marbling score was lower for TS+F than for TS. Percentage kidney, pelvic, and heart (KPH) linearly decreased with increasing shade. In contrast, KPH was greater for TS than for TS+F. Conclusion: Providing more than 2.4 ㎡ shade/animal will not further enhance feedlot performance. The use of fans in combination with shade increases ADG and gain efficiency beyond that of shade, alone. These enhancements were not associated with increased DMI, but rather, to an amelioration of ambient temperature humidity index on maintenance energy requirement.

Modeling of Fracture Toughness Test Procedures for Metal and Rock Materials using LS-DYNA (LS-DYNA를 이용한 금속 및 암석 재료의 파괴인성시험 모델링)

  • Choi, Byung-Hee;Ryu, Chang-Ha
    • Explosives and Blasting
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    • v.35 no.1
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    • pp.27-33
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    • 2017
  • In this study, two fracture toughness test procedures are modelled for selected metal and rock on LS-DYNA, which is a commercial finite element code. The tests are conducted by using the 3-point bend test procedure for rectangular bar specimen. Because it takes a relatively long time to conduct the test, the implicit solver based on the Newmark method is adopted for the analyses. The values of stress intensity factor obtained from the analyses are 73 and $0.3MPa.m^{0.5}$ for the metal and rock material, respectively. It can be thought that the resulting small value of the fracture toughness of the rock material model well represents the brittleness of rock material.

Transfer of Calcium along Trophic Levels on Limestone and Granitic Gneiss Grassland (석회암과 화강편마암 지역의 초지에서 영양단계별 칼슘 전이)

  • Yi, Hoon-Bok;Nam, Sang-Ho;Kim, Joon-Ho
    • Korean Journal of Environmental Biology
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    • v.25 no.3
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    • pp.228-238
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    • 2007
  • Calcium plays an important role for the organisms' physiology, reproduction, and growth. Calcium amount and transfer efficiency along trophic levels were compared at two different geological areas, Limestone area (LS) and Granitic Gneiss area (GG) in 1992 and 1993. Biomass and calcium amount of plants, herbivores and carnivores were seasonally measured. The removal sweeping net method was used to collect the quantitative insect samples. Calcium content (mgCa $g^{-1}$ DM) and pH of soil were 4.85 and 7.3 at LS and 0.21 and 7.3 at GG. The calcium transfer efficiencies (%) at LS and CG were 0.2 and 4.2 from soil to plants, 0.002 and 0.02 from plants to herbivores, and 73 and 47 from herbivores to carnivores, respectively. As a whole, the high calcium content of the LS soil reduced the utilization of calcium by plants. The higher trophical levels were, the higher ecological efficiency of the biological levels was. The calcium transfer amount was higher at LS, but its efficiency was rather higher at GG.

Effects of lactic acid bacteria inoculation in pre-harvesting period on fermentation and feed quality properties of alfalfa silage

  • Ertekin, Ibrahim;Kizilsimsek, Mustafa
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.2
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    • pp.245-253
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    • 2020
  • Objective: To develop the fermentation quality and chemical composition of alfalfa (Medicago sativa Lam.) silage, plants were inoculated with different lactic acid bacteria (LAB) strains at field 24 hours before harvest. Methods: The treatment groups were as follow: silage without additive as a control and inoculated with each strains of Lactobacillus brevis (LS-55-2-2), Leuconostoc citerum (L. citerum; L-70-6-1), Lactobacillus bifermentans (L. bifermentans; LS-65-2-1), Lactobacillus plantarum (L. plantarum; LS-3-3) and L. plantarum (LS-72-2). All the silages were stored at 25℃. Parameters such as pH, microorganism and volatile fatty acid contents, crude protein, neutral detergent fiber, acid detergent fiber, net gas, metabolizable energy, organic matter digestibility, dry matter intake and relative feed value were measured to determine fermentation quality, chemical compositions and relative feed value of alfalfa silages. Results: Significant differences were found among the control and treated groups in terms of pH and microorganism contents at all opening times and crude protein, net gas, metabolizable energy and organic matter digestibility of final silage. The pH values ranged from 4.70 to 5.52 for all treatments and control silage had the highest value of overall treatments at T75d silages. Volatile fatty acid of silages was not influenced significantly by inoculations. However, lactic acid content of L. bifermentans (LS-65-2-1) was higher than the other treatments. The highest metabolizable energy and organic matter digestibility were recorded from L. citerum (L-70-6-1) inoculation. In addition, no significant differences were found among treatments in terms of neutral detergent fiber, acid detergent fiber, dry matter intake and relative feed value. Conclusion: Among the treated LAB isolates, L. bifermentans came into prominence especially in terms of organic acid composition and quality characters of silages.

Evaluating Local Damages and Blast Resistance of RC Slabs Subjected to Contact Detonation (접촉 폭발 하중을 받는 RC 슬래브의 국부 손상 및 내폭 성능 평가)

  • Li, Ling;Lee, Jin Young;Min, Kyung Hwan;Yoon, Young Soo
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.17 no.1
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    • pp.37-45
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    • 2013
  • In this study, the resistance of various reinforced concrete (RC) slabs subjected to contact detonation was assessed. In order to enhance the blast resistance, fibers and external FRP sheets were reinforced to RC slabs. In the experiment, the $2,000{\times}1,000{\times}100mm$ sized RC slabs were fabricated using normal concrete (NC), steel fiber reinforced concrete (SFRC), polyvinyl alcohol fiber reinforced cementitious composite (PVA FRCC), and ultra-high performance cementitious composites (UHPCC). The damage levels of RC slabs subjected to contact detonation were evaluated by measuring the diameter and depth of crater, spall and breach. The experimental results were compared to the analyzed data using LS-DYNA program and three different prediction equations. The diameter and depth of crater, spall and breach were able to be predicted using LS-DYNA program approximately. The damage process of RC slabs under blast load was also well expressed. Three prediction equations suggested by other researchers had limitations to apply in terms of empirical approaches, therefore it needs further research to set more analytical considerations.

Establishment of a New Herbicides Screening Method Using Photoautotrophic Cultured Cell I. Studies on Culture Conditions for Developing Photoautotrophic Cells in Tobacco (광독립영양세포(光獨立營養細胞)를 이용(利用)한 새로운 제초제(除草劑) 선발법(選拔法) 확립(確立) I. 담배의 광독립영양세포(光獨立營養細胞) 육성(育成)을 위한 배양조건(培養條件)의 구명(究明))

  • Kim, K.U.;Suh, S.K.;Kwon, S.T.
    • Korean Journal of Weed Science
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    • v.11 no.1
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    • pp.68-73
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    • 1991
  • This study was conducted to determine the appropriate culture conditions for developing photoautotrophic cells from tobacco using plant growth regulators, sucrose and light condition in LS medium. The greatest callus induction was observed in the LS medium supplemented with $10^{-5}$ M NAA and $10^{-4}$ M BA, showing 3.08 g of callus fresh weight determined at 30 days after incubation. The highest chlorophyll content of callus was obtained in the LS medium supplemented with $10^{-5}$M NAA and $10^{-6}$ M BA, showing 28.42${\mu}g/g$. In both light and dark conditions, callus induction increased as the concentration of sucrose increased from 0.5%to 3.0%. In particular, in the light condition, the greatest callus induction was made in the LS medium supplemented with 2% sucrose. On the other hand, the heighest chlorophyll content was observed at 0.5% sucrose and chlorophyll wasn't induced in dark condition. The chlorophyll content of callus cultured in LS medium containing 0.75% sucrose was similar to 1% sucrose, as far as the chlorophyll content of callus was concerned.

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The Effect of Mixed Medicinal herb Extracts with Antimicrobial Activity on the Shelf-life of Kimchi (항균활성이 있는 한약재의 복합첨가가 김치 숙성에 미치는 영향)

  • Lee, Shin-Ho;Cho, Ok-Ki;Choi, Woo-Jeong;Kim, Soon-Dong
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1404-1408
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    • 1998
  • These studies were carried out to investigate the effect of mixed medicinal herbs, such as Curcuma longa and Lithospermum erythrorhozon (CL), Lithospermum erythrorhozon and Sophrora flavescens AITON (LS), Sophrora flavescens AITON and Glycyrrhiza uralensis (SG), and Glycyrrhiza uralensis and Schizandra chinensis (GS) on shelf-life of kimchi. The pH of kimchi containg 1% of medicinal herb extracts such as CL, LS, SG and GS was higher than that of control during fermentation for 25 days at $10^{\circ}C$ Titratable acidity, viable cells count of total bacteria and lactic acid bacteria in the kimchi were changed more slowly than in control. Shelf-life of kimchi was extended by addition of 1% mixed medicinal extracts such as CL, LS, SG and GS (1:1), respectively. The sensory quality (taste, flavor, crispness and overall acceptability) of CL, LS, SG and GS added kimchi was similar to that of control at 10 days of fermentation. But the sourness of LS, SG and GS (1%) added kimchi was more slowly developed than control after 15 days of fermentation, respectively. The effect of LS, SG and GS mixture on shelf-life in kimchi was significant. But the medicinal herb extracts added kimchi decreased its sensory quality with increasing concentration of the extracts.

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