• The Korean Journal of Physiology and Pharmacology
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• v.12 no.1
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• pp.31-35
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• 2008

Lysophosphatidylcholine (LPC), a metabolite of membrane phospholipids by phospholipase $A_2$, has been considered responsible for the development of abnormal vascular reactivity during atherosclerosis. $Ca^{2+}$ influx was shown to be augmented in atherosclerotic artery which might be responsible for abnormal vascular reactivity. However, the mechanism underlying $Ca^{2+}$ influx change in atherosclerotic artery remains undetermined. The purpose of the present study was to examine the effects of LPC on L-type $Ca^{2+}$ current $(I_{Ca(L)})$ activity and to elucidate the mechanism of LPC-induced change of $I_{Ca(L)}$ in rabbit portal vein smooth muscle cells using whole cell patch clamp. Extracellular application of LPC increased $I_{Ca(L)}$ through whole test potentials, and this effect was readily reversed by washout. Steady state voltage dependency of activation or inactivation properties of $I_{Ca(L)}$ was not significantly changed by LPC. Staurosporine (100 nM) or chelerythrine $(3{\mu}M)$, which is a potent inhibitor of PKC, significantly decreased basal $I_{Ca(L)}$, and LPC-induced increase of $I_{Ca(L)}$ was significantly suppressed in the presence of PKC inhibitors. On the other hand, application of PMA, an activator of PKC, increased basal $I_{Ca(L)}$ significantly, and LPC-induced enhancement of $I_{Ca(L)}$ was abolished by pretreatment of the cells with PMA. These findings suggest that LPC increased $I_{Ca(L)}$ in vascular smooth muscle cells by a pathway that involves PKC, and that LPC-induced increase of $I_{Ca(L)}$ might be, at least in part, responsible for increased $Ca^{2+}$ influx in atherosclerotic artery.

• Journal of Biomedical Engineering Research
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• v.30 no.1
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• pp.94-102
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• 2009

In this paper, we propose a hearing impairment simulator considering reduced frequency selectivity of the hearing impaired, and verify it's performance through experiments. The reduced frequency selectivity was embodied by spectral smearing using linear prediction coding(LPC). The experiments are composed of 4 kinds of tests; pure tone test, speech reception threshold(SRT) test, and word recognition score(WRS) test without spectral smearing and with spectral smearing. The experiments of the hearing impairment simulator were performed with 9 subjects who have normal hearing. The amount of spectral smearing was controlled by LPC order. The percentile score of WRS test without smearing is $89.78{\pm}2.420%$. The scores of WRS with 24th LPC order and with 8th LPC order are $88.00{\pm}3.556%$ and $83.78{\pm}2.123%$ respectively. It is verified that WRS score is lowered by decreasing LPC order. This is a reasonable result considering that spectral smearing is getting heavier according to decreasing LPC order. It is confirmed that spectral smearing using LPC simulates the reduced frequency selectivity of the hearing impaired and affects the clearness of speech reception.

• Bulletin of the Korean Chemical Society
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• v.27 no.8
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• pp.1149-1153
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• 2006

LPC analogues including natural and unnatural LPC, 3-L-2-PC, acetylated LPC and ethylene glycol derivative are prepared from D-mannitol using in convenient procedures by only changing the synthetic sequences, and their protective activities against cecal ligation and puncture (CLP)-induced severe sepsis are compared. The chirality at C2 position in LPC is found to be required as (S)-configuration for sepsis inhibition, comparing from the protection activity between LPC 6 and unnatural LPC 8. The hydroxyl functionality is also very important and required at C2 or C3 position as shown in the protection activities of ethylene glycol analogue 11 and 3-L-2-PC 9.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.4C
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• pp.397-402
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• 2009

Accurate estimation of time-selective fading channel is a difficult problem in OFDM(Orthogonal Frequency Division Multiplexing) system. There are many channel estimation algorithms that are very weak in noisy channel. For solving this problem, we use EM (Expectation-Maximization) algorithm for iterative optimization of the data. We propose an EM-LPC algorithm to estimate the time-selective fading. The proposed algorithm improves of the BER performance compared to EM based channel estimation algorithm and reduces the iteration number of the EM loop. We simulated the uncoded system. If coded system use the EM-LPC algorithm, the performance are enhanced because of the coding gain. The EM-LPC algorithm is able to apply to another communication system, not only OFDM systems. The image processing of the medical instruments that the demand of accurate estimation can also use the proposed algorithm.

• Journal of the Institute of Convergence Signal Processing
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• v.6 no.3
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• pp.143-147
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• 2005

Monitoring and diagnosis of the operating machines are very important for safety operation and maintenance in the industrial fields. These machines are most rotating machines and the diagnosis of the machines has been researched for long time. We can easily see the faulted signal of the rotating machines from the changes of the signals in frequency. The Linear Predictive Coding(LPC) is introduced for signal analysis in frequency domain. In this paper, we propose fault detection and diagnosis method using the Linear Predictive Coding(LPC) and residual signal energy. We applied our method to the induction motors depending on various status of faulted condition and could obtain good results.

• Proceedings of the KIEE Conference
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• 1987.07b
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• pp.881-883
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• 1987

This paper decribes a new technique for the generation of excitation sources in LPC system. We synthesize a speech signal using several excitation sources, according to residual signal energy and ZCR(zero Crossing Rate). One of the excitation sources mix the double differentiated glottal wave form source and noise source. As a result, we got improved speech signal than that produced by conventional LPC system.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.1
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• pp.27-32
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• 2009

The effects of oxidized low-density lipoprotein(OxLDL) and its major lipid constituent lysophosphatidylcholine(LPC) on $Ca^{2+}$ entry were investigated in cultured human umbilical endothelial cells(HUVECs) using fura-2 fluorescence and patch-clamp methods. OxLDL or LPC increased intracellular $Ca^{2+}$ concentration($[Ca^{2+}]_i$), and the increase of $[Ca^{2+}]_i$ by OxLDL or by LPC was inhibited by $La^{3+}$ or heparin. LPC failed to increase $[Ca^{2+}]_i$ in the presence of an antioxidant tempol. In addition, store-operated $Ca^{2+}$ entry(SOC), which was evoked by intracellular $Ca^{2+}$ store depletion in $Ca^{2+}$-free solution using the sarcoplasmic reticulum $Ca^{2+}$ pump blocker, 2, 5-di-t-butyl-l,4-benzohydroquinone(BHQ), was further enhanced by OxLDL or by LPC. Increased SOC by OxLDL or by LPC was inhibited by U73122. In voltage-clamped cells, OxLDL or LPC increased $[Ca^{2+}]_i$ and simultaneously activated non-selective cation(NSC) currents. LPC-induced NSC currents were inhibited by 2-APB, $La^{3+}$ or U73122, and NSC currents were not activated by LPC in the presence of tempol. Furthermore, in voltage-clamped HUVECs, OxLDL enhanced SOC and evoked outward currents simultaneously. Clamping intracellular $Ca^{2+}$ to 1 ${\mu}M$ activated large-conductance $Ca^{2+}$-activated $K^+(BK_{ca})$ current spontaneously, and this activated $BK_{ca}$ current was further enhanced by OxLDL or by LPC. From these results, we concluded that OxLDL or its main component LPC activates $Ca^{2+}$-permeable $Ca^{2+}$-activated NSC current and $BK_{ca}$ current simultaneously, thereby increasing SOC.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.1
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• pp.25-30
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• 2006

Lysophosphatidylcholine (LPC), which accumulates in atherosclerotic arteries, has been reported to inhibit endothelium-dependent relaxation (EDR) in many different species. However, the underlying mechanism of LPC-induced inhibition of EDR is still uncertain. In the present study, we measured simultaneously both isometric tension and cytosolic free $Ca^{2+}$ ($[Ca^{2+}]_i$) in rabbit carotid strips, and examined the effect of LPC on tension and $[Ca^{2+}]_i$. In carotid strips with intact-endothelium, high $K^+$ (70 mM) increased both tension and $[Ca^{2+}]_i$, and cumulative addition of acetylcholine (ACh) from 0.1 to $10{\mu}M$ induced dose dependent increase of $[Ca^{2+}]_i$ with concomitant relaxation. In the presence of L-NAME (0.1 mM), ACh increased $[Ca^{2+}]_i$ without affecting the amplitude of high $K^+-induced$ tension. These ACh-induced change of $[Ca^{2+}]_i$ and tension was abolished by removal of endothelium or 10 nM 4-DAMP (muscarinic receptor antagonist) pretreatment. Pretreatment of LPC ($10{\mu}M$) inhibited ACh ($10{\mu}M$)-induced change of tension and $[Ca^{2+}]_i$ in endothelium-intact carotid artery. On the other hand, LPC had no effect on ACh-induced change of tension and $[Ca^{2+}]_i$ in endothelium denuded artery. In $Ca^{2+}$-free external solution, ACh transiently increased $[Ca^{2+}]_i$, and pretreatment of LPC significantly inhibited ACh-induced transient $[Ca^{2+}]_i$ change. Based on the above results, it may be concluded that LPC inhibits the ACh-induced $[Ca^{2+}]_i$ change through inhibition of $Ca^{2+}$ mobilization in vascular endothelial cells, resulting in decreased production of NO and concomitant inhibition of endotheliumdependent vascular relaxation.

• Proceedings of the Acoustical Society of Korea Conference
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• 1987.11a
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• pp.82-85
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• 1987

Preliminary results of using the LPC parameter for text-independent speaker identification problem are presented. The idetification process includes log likelihood ratio for distance measure and dynamic programming for time normalization. To generate the data base for experiments, ten times. Experimental results show 99.4% of identification accuracy, incorrect identification were made when the speaker uses a dialect.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2013.06a
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• pp.6-7
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• 2013

저전송률 오디오 부호화기의 성능 향상을 위해 가변 LPC 기반으로 스펙트럼을 평탄화 하는 방법을 제안한다. 제안한 방법은 대역별 scale factor를 동일하게 하여 비트 효율을 증가시키고 spectral hole이 발생하는 문제점을 해결할 수 있다. 또한, 가변 LPC 필터를 사용하여 프레임 특성에 따라 스펙트럼 평탄화 강도를 가변적으로 조절하여 성능 향상을 제공한다. 제안한 방법이 일반 LPC 필터 방법보다 저대역의 부호화 성능을 향상시키고 스테레오 왜곡을 감소시키는 것을 확인하였다.