• Molecules and Cells
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• 제39권11호
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• pp.834-840
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• 2016

Caenorhabditis elegans (C. elegans) utilizes two different cell-cycle modes, binucleations during the L1 larval stage and endoreduplications at four larval moltings, for its postembryonic intestinal development. Previous genetic studies indicated that CDC-25.2 is specifically required for binucleations at the L1 larval stage and is repressed before endoreduplications. Furthermore, LIN-23, the C. elegans ${\beta}$-TrCP ortholog, appears to function as a repressor of CDC-25.2 to prevent excess intestinal divisions. We previously reported that intestinal hyperplasia in lin-23(e1883) mutants was effectively suppressed by the RNAi depletion of cdc-25.2. Nevertheless, LIN-23 targeting CDC-25.2 for ubiquitination as a component of E3 ubiquitin ligase has not yet been tested. In this study, LIN-23 is shown to be the major E3 ubiquitin ligase component, recognizing CDC-25.2 to repress their activities for proper transition of cell-cycle modes during the C. elegans postembryonic intestinal development. In addition, for the first time that LIN-23 physically interacts with both CDC-25.1 and CDC-25.2 and facilitates ubiquitination for timely regulation of their activities during the intestinal development.

• Bulletin of the Korean Chemical Society
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• 제23권4호
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• pp.617-618
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• 2002

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.23-30
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• 2019

Objective: Recent studies have demonstrated that lin-28 homolog B (LIN28B)/miRNA let-7 (let-7) plays a role in the regulation of pubertal onset in mammals. However, the role of LIN28B/let-7 in the onset of ovine puberty remains unknown. We cloned the Duolang sheep Lin28B cDNA sequence, detected the expression change of LIN28B, let-7a and let-7g in hypothalamus, pituitary and ovary tissues at three different pubertal stages. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of LIN28B gene from Duolang sheep and the bioinformatics methods were applied to analyze the amino acid sequence of LIN28B protein. The mRNA expression levels of the LIN28B gene at different pubertal stages were examined by real time RT-PCR. Results: LIN28B cDNA of Duolang sheep was cloned, and two transcripts were obtained. The amino acid sequence of transcript 1 shares 99.60%, 98.78%, and 94.80% identity with those of goat, wild yak and pig, respectively. Strong LIN28B mRNA expression was detected in the hypothalamus, pituitary, ovary, oviduct and uterus, while moderate expression was found in the liver, kidney, spleen and heart, weak expression was observed in the heart. No expression was found in the lungs. Quantitative real-time PCR (QPCR) and western-blot analysis revealed that the LIN28B was highly expressed in the hypothalamus and ovary at prepuberty stages, and this expression significantly decreased from the prepuberty to puberty stages (p<0.05). Markedly increased levels of mRNA expression were detected in the pituitary from prepuberty to puberty (p<0.05) and then significantly decreased from puberty to post-puberty (p<0.05). The expression levels of let-7a and let-7g showed no significant changes among different pubertal stages (p>0.05). Conclusion: These results provided a foundation for determining the functions of LIN28B/let-7 and their role in the onset of sheep puberty.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2007년도 The 1st International Symposium on Advanced Magnetic Materials
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• pp.23.1-23.1
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• 2007

• 한국진공학회:학술대회논문집
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• 한국진공학회 2001년도 창립10주년 제20회 학술발표회
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• pp.23-24
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• 2001

• Bulletin of the Korean Chemical Society
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• 제23권6호
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• pp.899-903
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• 2002

• IMMUNE NETWORK
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• 제23권4호
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• pp.34.1-34.15
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• 2023

Lung cancer, particularly non-small cell lung cancer (NSCLC) which contributes more than 80% to totally lung cancer cases, remains the leading cause of cancer death and the 5-year survival is less than 20%. Continuous understanding on the mechanisms underlying the pathogenesis of this disease and identification of biomarkers for therapeutic application and response to treatment will help to improve patient survival. Here we found that a molecule known as DUSP10 (also known as MAPK phosphatase 5) is oncogenic in NSCLC. Overexpression of DUSP10 in NSCLC cells resulted in reduced activation of ERK and JNK, but increased activation of p38, which was associated with increased cellular growth and migration. When inoculated in immunodeficient mice, the DUSP10-overexpression NSCLC cells formed larger tumors compared to control cells. The increased growth of DUSP10-overexpression NSCLC cells was associated with increased expression of tumor-promoting cytokines including IL-6 and TGFβ. Importantly, higher DUSP10 expression was associated with poorer prognosis of NSCLC patients. Therefore, DUSP10 could severe as a biomarker for NSCLC prognosis and could be a target for development of therapeutic method for lung cancer treatment.

• 한국도로학회:학술대회논문집
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• 한국도로학회 2010년 춘계학술대회 논문집
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• pp.19-23
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• 2010

• 정보보호학회논문지
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• 제23권3호
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• pp.359-370
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• 2013

2010년, Lin과 Chan은 가역 비밀이미지 공유(reversible secret image sharing) 기법을 최초로 제안했다. 이 기법의 장점은 쉐도우 이미지(shadow images)의 왜곡 비율(distortion ratio)이 작고, 비밀이미지의 삽입량(embedding capacity)이 기존의 기법들에 비해 높으며, 가역(reversible)이 가능하다. 그러나 그들의 기법은 몇 가지 문제점들이 존재한다. 첫째, 나머지 연산(modular operation)에 사용하는 소수 m에 의하여 전체 참가자들의 수가 제한된다. 둘째, 비밀 공유 과정 내 양자화 값(quantized value)과 다항식의 결과 값의 덧셈 연산에서 오버플로우(overflow)가 발생한다. 마지막으로, 다항식 최고차항의 계수가 0이 되어 t-1명의 참가자로도 비밀데이터 접근이 가능해지는 문제점을 가진다. 본 논문에서는 Lin과 Chan이 제안한 기법의 문제점을 해결하는 동시에 쉐도우 이미지의 왜곡 비율이 작고 비밀 이미지의 삽입량을 향상시키는 기법을 제안한다. GF($2^8$)상에서의 다항식 연산을 통해 전체 참가자 수의 제한과 오버플로우 문제를 해결하고, 다항식 최고차항의 계수 중 MSB 4-비트를 고정하는 방법을 적용하여 계수가 0이 될 수 있는 문제점을 해결한다. 실험결과에서는 Lin과 Chan의 기법에서 PSNR과 삽입량이 서로 반비례하지만 제안한 기법의 경우 삽입량이 증가하더라도 PSNR은 45dB 이상으로 유지됨을 알 수 있다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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• pp.776-779
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• 2006

The alignment of liquid crystal (LC) is one of the key issues of liquid crystal display (LCD) technologies. In this study, we aligned polyimide (as the LC alignment layer) by ALT plasma beam and to realize the stability and characteristics of this technology. The characteristics such as anchoring energy, pre-tilt angle, voltage holding ratio(VHR) and residual direct current(Rdc) were discussed. Besides, we applied it to the in plane switch (IPS) mode 23" WXGA real panel, the performance parameters and electrical properties were measured and compared with those of rubbing alignment. From the result, we demonstrated a successful LC alignment treatment process in real panel by ALT plasma beam.