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http://dx.doi.org/10.14348/molcells.2016.0238

LIN-23, an E3 Ubiquitin Ligase Component, Is Required for the Repression of CDC-25.2 Activity during Intestinal Development in Caenorhabditis elegans  

Son, Miseol (Department of Bioscience and Biotechnology, Konkuk University)
Kawasaki, Ichiro (Department of Bioscience and Biotechnology, Konkuk University)
Oh, Bong-Kyeong (Institute of Medical Science, Hanyang University College of Medicine)
Shim, Yhong-Hee (Department of Bioscience and Biotechnology, Konkuk University)
Publication Information
Molecules and Cells / v.39, no.11, 2016 , pp. 834-840 More about this Journal
Abstract
Caenorhabditis elegans (C. elegans) utilizes two different cell-cycle modes, binucleations during the L1 larval stage and endoreduplications at four larval moltings, for its postembryonic intestinal development. Previous genetic studies indicated that CDC-25.2 is specifically required for binucleations at the L1 larval stage and is repressed before endoreduplications. Furthermore, LIN-23, the C. elegans ${\beta}$-TrCP ortholog, appears to function as a repressor of CDC-25.2 to prevent excess intestinal divisions. We previously reported that intestinal hyperplasia in lin-23(e1883) mutants was effectively suppressed by the RNAi depletion of cdc-25.2. Nevertheless, LIN-23 targeting CDC-25.2 for ubiquitination as a component of E3 ubiquitin ligase has not yet been tested. In this study, LIN-23 is shown to be the major E3 ubiquitin ligase component, recognizing CDC-25.2 to repress their activities for proper transition of cell-cycle modes during the C. elegans postembryonic intestinal development. In addition, for the first time that LIN-23 physically interacts with both CDC-25.1 and CDC-25.2 and facilitates ubiquitination for timely regulation of their activities during the intestinal development.
Keywords
C. elegans; CDC-25.2; E3 ubiquitin ligase; intestinal development; LIN-23;
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