• Korean Journal Plant Pathology
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• v.13 no.1
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• pp.1-4
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• 1997

Viroid-like RNA molecules were detected from the low molecular weight RNAs isolated from the Korean peonies which showed typical viroid symptoms of epinasty and dwarfing. Low molecular weight RNAs including viroid RNA molecules were purified by the Qiagen anion exchange minicolumns. Viroid-like RNA molecules showed a single viroid specific band in the native polyacrylamide gel. They were separated into two bands in the denaturing gel conditions. The band of circular form of viroid-like RNAs was crossed over the horizontal band of the linear form of viroid-like RNA molecules in 0~8 M urea gradient gel under the denaturing conditions of 37$^{\circ}C$. The two circular forms of viroid-like RNA molecules were detected in the reverse polyacrylamide gel electrophoresis. The viroid-like RNA molecules purified from the peonies were supposed to be unidentified viroid RNA molecules.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1647-1652
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• 2010

Autophagy refers to the bulk degradation of cellular proteins and organelles through an autophagosome and plays a pivotal role in the development, cellular differentiation, aging, and elimination of aberrant structures. A failure of autophagy has been implicated in a growing list of mammalian disease states, including cancer and cardiomyopathy. Two ubiquitin-like systems are highly involved in autophagy, especially in the formation of autophagosomes. Here, we purified and characterized Atg7 (an E1-like enzyme), and Atg3 and Atg10 (E2-like enzymes) in order to gain an insight into the role played by ubiquitin-like systems in the formation of autophagosomes. Interestingly, we observed that Atg7 forms a homodimer to construct an active conformation, unlike other E1-like enzymes. Although Atg3 was detected as a monomer under physiological conditions, Atg10 existed in an oligomeric form, indicating that the mechanism by which Atg10 functions may differ from that of Atg3.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.71.1-71.1
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• 2017

WouldYouLike[우주라이크]는 2009년 천문학 전공 대학생들을 중심으로 탄생한 천문우주과학 대중화를 위해 활동하는 비영리 단체이다. 천문우주과학에 대한 정보를 얻기 힘든 사람들을 위해 쉽게 접할 수 있는 콘텐츠를 제작하여 이를 무료로 배포하고 있다. 2012년 무료 배포용 천문학 잡지 우주라이크 창간호를 시작으로 2017년 현재 11호까지 발간되었으며, 이 잡지는 전국 각지의 대학교 캠퍼스와 천문대 및 과학관의 협조를 통해 배포 중이다. 이러한 오프라인 활동과 더불어 SNS(인스타그램, 페이스북)와 홈페이지를 비롯해 현대인들에게 접근성이 높은 온라인 매체를 적극적으로 활용한 천문학 콘텐츠와 팟캐스트 등을 통해 대중에게 다가가고 있다. 특히 우주라이크 페이스북 페이지의 팔로워 수는 약 35,000명이며, 이는 한국천문연구원 페이스북 페이지의 약 6,000명보다 많은 수치이다. 이는 우주라이크 본연의 목적인 천문학 대중화에 우주라이크가 크게 기여하고 있음을 보여준다. 현재 우주라이크는 상기에 기술한 활동들과 함께 NASA에서 운영하는 APOD (Astronomy Picture of the Day)의 한글판 서비스를 공식적으로 번역하여 제공하고 있다. 이 포스터는 국내에서 천문우주과학 대중화의 필요성을 역설하며, 이와 연계해서 우주라이크의 활동 내역과 현재 추진 중인 계획을 소개한다. 그리고 이에 대한 국내 천문학계의 다양한 목소리를 듣고 추후 활동에 반영하고자 한다.

• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.139-144
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• 2005

This experiment was conducted to identify the effect of several plant growth retardants on endogenous ABA-like substance content and GA-like substance activity in seedlings of Sedirea japonica cultured in vitro. When seedlings of Sedirea japonica were treated with low concentration of 0.05 mg/L Uniconazole, 0.1 mg/L Ancymidol and 0.3 mg/L Paclobutrazol, the content of ABA-like substances of the leaf was lower than that of the control. However, the activity of GA-like substances was similar or higher in treated seedlings. In the mid and high concentrations of three kinds of growth retardants, the ABA-like substance content was increased, but GA-like substance activity was inhibited. The content of ABA-like substances in the root was lower in 0.05 and 0.2 mg/L Uniconazole, 0.2 mg/L Ancymidol and 0.1 mg/L Paclobutrazol treatments than that of the control, but in the mid and high concentration treatments, the content was increased. GA-like substance activity in low concentration was increased but in the mid and high concentration, the activity was inhibited compared with low concentration treatment.

• The KIPS Transactions:PartA
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• v.19A no.4
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• pp.175-180
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• 2012

This paper describes the design of high-performance pedestrian and vehicle detection circuit using the Haar-like features. The proposed circuit uses a sliding window for every image frame in order to extract Haar-like features and to detect pedestrians and vehicles. A total of 200 Haar-like features per sliding window is extracted from Haar-like feature extraction circuit and the extracted features are provided to AdaBoost classifier circuit. In order to increase the processing speed, the proposed circuit adopts the parallel architecture and it can process two sliding windows at the same time. We described the proposed high-performance pedestrian and vehicle detection circuit using Verilog HDL and synthesized the gate-level circuit using the 130nm standard cell library. The synthesized circuit consists of 1,388,260 gates and its maximum operating frequency is 203MHz. Since the proposed circuit processes about 47.8 $640{\times}480$ image frames per second, it can be used to provide the real-time detection of pedestrians and vehicles.

• Steel and Composite Structures
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• v.21 no.3
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• pp.501-515
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• 2016

Seismic analysis for steel frame structure with brace configuration using topology optimization based on truss-like material model is studied. The initial design domain for topology optimization is determined according to original steel frame structure and filled with truss-like members. Hence the initial truss-like continuum is established. The densities and orientation of truss-like members at any point are taken as design variables in finite element analysis. The topology optimization problem of least-weight truss-like continuum with stress constraints is solved. The orientations and densities of members in truss-like continuum are optimized and updated by fully-stressed criterion in every iteration. The optimized truss-like continuum is founded after finite element analysis is finished. The optimal bracing system is established based on optimized truss-like continuum without numerical instability. Seismic performance for steel frame structures is derived using dynamic time-history analysis. A numerical example shows the advantage for frame structures with brace configuration using topology optimization in seismic performance.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.1
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• pp.43-48
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• 2013

Adaboost is widely used for Haar-like feature boosting algorithm in Face Detection. It shows very effective performance on single distribution model. But when detecting front and side face images at same time, Adaboost shows it's limitation on multiple distribution data because it uses linear combination of basic classifier. This paper suggest the HDCT, modified decision tree algorithm for Haar-like features. We still tested the performance of HDCT compared with Adaboost on multiple distributed image recognition.

• Reproductive and Developmental Biology
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• v.32 no.4
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• pp.211-215
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• 2008

The HMG box containing protein (HBP) has a high mobility group domain and involved in the regulation of proliferation and differentiation of tissues. We screened HBP2 in glioblastoma using Suppression Subtractive Hybridization (SSH) and isolated human spermatogonial stem cell-like cells (hSSC-like cells) derived from patients of nonobstructive azoospermia (NOA). Expression of HBP2 was analyzed by RT-PCR in undifferentiated stem cells (human Embryonic Stem Cells, hSSC-like cells 2P) and spontaneous differentiated stem cells (hSSC-like cells 4P). It was overexpressed in hESC and hSSC-like cells 2P but not in hSSC-like cells 4P. Also, the expression level of HBP2 was downregulated in colon tumor tissues compared to normal tissues. Specifically in synchronized WI-38 cells, HBP2 was highly upregulated until the G1 phase of the cell cycle and gradually decreased during the S phase. Our results suggest that HBP2 was downregulated during the spontaneous differentiation of hSSC-like cells. HBP2 was differently expressed in colon tissues and was related to G1-progression in WI-38 cells. It may playa role in the maintenance of an undifferentiated hSSC-like cell state and transits from G1 to S in WI-38 cells. This research was important that it identified a biomarker for an undifferentiated state of hSSC-like cells and characterized its involvement to arrest during cell cycle in colon cancer.

• Environmental Engineering Research
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• v.11 no.3
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• pp.127-133
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• 2006

Aerobic floc-like sludge was formed in a batch reactor and the effect of cations on the formation of aerobic floc-like sludge was studied. In order to enhance the formation (rate) of aerobic floc-like sludge, cations such as $K^+$, $Na^+$, $Ca^{2+}$, and $Mg^{2+}$ were added to the seed sludge. It was found that $Ca^{2+}$ had positive effect on the formation of floc-like sludge, as measured by sludge volume index (SVI) for settle ability. The formation of floc-like sludge was confirmed by the microscopic observation after DAPI staining. The scattered forms of sludge samples at the initial stage became aggregated to form floes after $Ca^{2+}$ addition. To ensure the functions of sludge floes in a treatment plant, the gradient of ionic species around the surfaces of floc-like sludge was monitored by ion selective microelectrodes for ${NH_4}^+,\;{NO_3}^-$, and pH. The effective concentration of $Ca^{2+}$ ion to form floc-like sludge was determined to be 750 mg/L (0.15 mg $Ca^{2+}/mg$ MLSS). Under the effective $Ca^{2+}$ condition, the SVI value was the lowest and large distribution of nitrifying bacteria at the outer surface was observed in the aerobic floc-like sludge. From the results, it was found that the calcium ion functioned as an agent for the formation of aerobic floc-like sludge, resulting in the enhanced nitrification.

• Journal of Korea Game Society
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• v.20 no.2
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• pp.35-44
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• 2020

This study proposes the method for converging Buddhist Worldview into games. The Rogue-like game "Dungeed" and "Nectar Ritual Painting" were selected for analysis. Next, the characteristics of the Buddhist worldview in Nectar Ritual Painting and the Rogue-like game Dungeed were compared and analyzed. As a result, this study proposed game elements of Rogue-like that analyzed in the characteristics of Buddhist world view. In the future, we will examine the expandability of the game through the Buddhist Worldview.