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Screening and functional validation of lipid metabolism-related lncRNA-46546 based on the transcriptome analysis of early embryonic muscle tissue in chicken

  • Ruonan, Chen;Kai, Liao;Herong, Liao;Li, Zhang;Haixuan, Zhao;Jie, Sun
    • Animal Bioscience
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    • v.36 no.2
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    • pp.175-190
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    • 2023
  • Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

Improvement Scheme of Simplified Liquefaction Potential Evaluation for a Dredged and Reclaimed Ground (준설매립지반의 액상화 간편예측 개선에 관한 연구)

  • Jung, Min-Hyung;Kim, Ju-Hyun;Jeong, Sang-Guk;Lee, Song
    • Journal of the Korean Geotechnical Society
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    • v.23 no.8
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    • pp.47-57
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    • 2007
  • In this study, the analysis of density characteristics of some dredged and reclaimed ground sites, which is necessary for liquefaction evaluation of a dredged and reclaimed ground, was conducted. From analyzing a simplified liquefaction potential evaluation using SPT-N values which have been applied as domestic earthquake-resistant design criterion, improvement scheme is suggested. Based on the analysis result of density characteristics, it was found out that the relative density and the intial N-value ranged respectively $40{\sim}50%\;and\;5{\sim}8$. In the case of applying Liao & Whitman's equation to correct effective overburden pressure, liquefaction resistance of the upper ground that is relatively weaker than that of lower ground is overestimated. So, Skempton's equation is recommended. And the N value with depth which is applied for design process should be estimated by the exponential equation, $N=1.35{\sigma}'^{0.75}$.

Effect of soil-structure interaction on the reliability of hyperbolic cooling towers

  • Liao, Wen;Lu, Wenda;Liu, Renhuai
    • Structural Engineering and Mechanics
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    • v.7 no.2
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    • pp.217-224
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    • 1999
  • A semi-stochastic process model of reliability was established for hyperbolic cooling towers subjected to combined loadings of wind force, self-weight, temperature loading. Effect of the soil-structure interaction on reliability was evaluated. By involving the gust factor, an equivalent static scheme was employed to convert the dynamic model to static model. The TR combination rule was used to consider relations between load responses. An analysis example was made on the 90M cooling tower of Maoming, Guangdong of China. Numerical results show that the design not including interaction turns to be conservative.

Input Voltage Sharing Control for Input-Series-Output-Parallel DC-DC Converters without Input Voltage Sensors

  • Guo, Zhiqiang;Sha, Deshang;Liao, Xiaozhong
    • Journal of Power Electronics
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    • v.12 no.1
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    • pp.83-87
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    • 2012
  • Input-series-output-parallel (ISOP) modular converters consisting of multiple modular DC/DC converters can enable low voltage rating switches for use in high voltage input applications. In this paper, an input voltage sharing control strategy for input-series-output-parallel (ISOP) full-bridge (FB) DC/DC converters is proposed. By sensing the difference in the input current of two modules, the system can achieve input voltage sharing for DC-DC modules. The effectiveness of the proposed control strategy is verified by simulation and experimental results obtained with a 200w-50kHz prototype.

High-Performance Spatial and Temporal Error-Concealment Algorithms for Block-Based Video Coding Techniques

  • Hsu, Ching-Ting;Chen, Mei-Juan;Liao, Wen-Wei;Lo, Shen-Yi
    • ETRI Journal
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    • v.27 no.1
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    • pp.53-63
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    • 2005
  • A compressed video bitstream is sensitive to errors that may severely degrade the reconstructed images even when the bit error rate is small. One approach to combat the impact of such errors is the use of error concealment at the decoder without increasing the bit rate or changing the encoder. For spatial-error concealment, we propose a method featuring edge continuity and texture preservation as well as low computation to reconstruct more visually acceptable images. Aiming at temporal error concealment, we propose a two-step algorithm based on block matching principles in which the assumption of smooth and uniform motion for some adjacent blocks is adopted. As simulation results show, the proposed spatial and temporal methods provide better reconstruction quality for damaged images than other methods.

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Bidirectional Hybrid DWDM-PON for HDTV/Gigabit Ethernet/CATV Applications

  • Lu, Hai-Han;Tsai, Wen-Shing;Chien, Tzu-Shen;Chen, Shih-Hung;Chi, Yu-Chieh;Liao, Che-Wei
    • ETRI Journal
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    • v.29 no.2
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    • pp.162-168
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    • 2007
  • A new scheme for bi-directional HDTV/Gigabit Ethernet/CATV transmission over a hybrid dense-wavelength-division-multiplexing passive optical network (DWDM-PON) is proposed and demonstrated. It is based on injection-locked vertical-cavity surface-emitting lasers and distributed-feedback laser diodes as transmitters. Services with 129 HDTV channels, a 1.25 Gbps Gigabit Ethernet connection, and 77 CATV channels are successfully demonstrated over 40 km single-mode fiber links. Good performance of bit error rate, carrier-to-noise ratio, composite second order, and composite triple beat is achieved in our proposed bidirectional DWDM-PON.

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Opportunistic Scheduling with QoS Constraints for Multiclass Services HSUPA System

  • Liao, Dan;Li, Lemin
    • ETRI Journal
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    • v.29 no.2
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    • pp.201-211
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    • 2007
  • This paper focuses on the scheduling problem with the objective of maximizing system throughput, while guaranteeing long-term quality of service (QoS) constraints for non-realtime data users and short-term QoS constraints for realtime multimedia users in multiclass service high-speed uplink packet access (HSUPA) systems. After studying the feasible rate region for multiclass service HSUPA systems, we formulate this scheduling problem and propose a multi-constraints HSUPA opportunistic scheduling (MHOS) algorithm to solve this problem. The MHOS algorithm selects the optimal subset of users for transmission at each time slot to maximize system throughput, while guaranteeing the different constraints. The selection is made according to channel condition, feasible rate region, and user weights, which are adjusted by stochastic approximation algorithms to guarantee the different QoS constraints at different time scales. Simulation results show that the proposed MHOS algorithm guarantees QoS constraints, and achieves high system throughput.

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Chemical Modification of Poly(vinylacetylene) and Synthesis of Poly(1,2,3-triazole)s

  • Wang, Lian-Jun;Liao, Shan;Wang, Le-Yong
    • Bulletin of the Korean Chemical Society
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    • v.32 no.5
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    • pp.1471-1474
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    • 2011
  • A series of new polymers containing 1,2,3-triazoles were synthesized and their structures and properties were characterized by FT-IR, $^1H$ NMR, TGA, DSC and GPC. The results showed that the polymers modified by alkyl groups had good solubility, thermal stability and reasonable molecular weights. It was also demonstrated that the properties of fluorine-containing polymers were seriously affected by fluorine atoms with hydrophobic and chemical proof properties.

A CLASS OF NEW NEAR-PERFECT NUMBERS

  • LI, YANBIN;LIAO, QUNYING
    • Journal of the Korean Mathematical Society
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    • v.52 no.4
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    • pp.751-763
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    • 2015
  • Let ${\alpha}$ be a positive integer, and let $p_1$, $p_2$ be two distinct prime numbers with $p_1$ < $p_2$. By using elementary methods, we give two equivalent conditions of all even near-perfect numbers in the form $2^{\alpha}p_1p_2$ and $2^{\alpha}p_1^2p_2$, and obtain a lot of new near-perfect numbers which involve some special kinds of prime number pairs. One kind is exactly the new Mersenne conjecture's prime number pair. Another kind has the form $p_1=2^{{\alpha}+1}-1$ and $p_2={\frac{p^2_1+p_1+1}{3}}$, where the former is a Mersenne prime and the latter's behavior is very much like a Fermat number.

Single-panel simulation on liquid crystal on silicon

  • Liao, Engle;Chiu, Jack;Peng, James
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.939-942
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    • 2004
  • In this study, we report simulation results of single-panel LCOS (liquid crystal on silicon). Reflective LCOS microdisplays are widely used in various projection and near-eye application. For one panel system, liquid crystal response time is an important variable. The panel must switch fast enough to support the display of Field color sequential with high field rates. In order to have fast response and good contrast, a vertical alignment (VA) cell was used in this study. With suitable selection on LC parameters like temperature, viscosity, elastic constant and birefringence, it is possible to get response time of around 2ms from a 2.0 um-thick vertical alignment cell. This result also indicates an ease of production control on 2.0 um cells than 1.0 um cells.

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