• Title/Summary/Keyword: LED fluorescence

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A STUDY ON THE RELIABILITY OF THE OPTICAL CARIES ACTIVITY TEST (광학적 치아우식활성 검사법의 신뢰도에 관한 연구)

  • Park, Cheol-Hong;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.4
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    • pp.615-623
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    • 2006
  • The purpose of this study was to evaluate the specificity, sensitivity, and diagnostic power of caries activity test using LED fluorescence. The subjects of this study were 55 children of $6{\sim}7$ years old. LED light were irradiated to labial or buccal surface of all teeth. Fluorescence from initial carious lesion of teeth illuminated by an LED light was observed through barrier filter and the number of teeth showing lesion, size and position of lesion were counted. Streptococcus mutans colony counting and dDfFtT rate test were also done and their correlation was compared. And then specificity, sensitivity, diagnostic power of optical caries activity test using LED light were evaluated. 1. There was positive $correlation({\gamma}=0.43)$ between LED fluorescence test and Streptococcus mutans count(P<0.05). 2. When visual examination was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 76.1%, and 100%. 3. When dDfFtT rate was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 88.9%, 47.8%, and 95.7%. 4. When S. mutans colony counting was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 58.7%, and 100%. Considering the above results, optical caries activity test using LED light could be regarded as a practical method because of its close relationship with microbiological caries activity test.

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Development of Photo-sensor for Integrated Lab-On-a-Chip (집적화된 Lab-On-a Chip을 위한 광센서의 제작 및 특성 평가)

  • 김주환;신경식;김용국;김태송;김상식;주병권
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.4
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    • pp.404-409
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    • 2004
  • We fabricated photo-sensor for fluorescence detection in LOC. LOC is high throughput screening system. Our LOC screens biochemical reaction of protein using the immunoassay, and converts biochemical reaction into electrical signal using LIF(Laser Induced Fluorescence) detection method. Protein is labeled with rhodamine intercalating dye and finger PIN photodiode is used as photo-sensor We measured fluorescence emission of rhodamine dye and analyzed tendency of fluorescence detection, according to photo-sensor size, light intensity, and rhodamine concentration. Detection current was almost linearly proportional to two parameters, intensity and concentration, and was inversely proportional to photo-sensor size. Integrated LOC consists of optical-filter deposited photo-sensor and PDMS microchannel detected 50 (pg/${mu}ell$) rhodamine. For integrated LOC including light source, we used green LED as the light source and measured emitted fluorescence.

Quinine Assay with Home-Built UV-LED Fluorometer: Quantitative Analysis, Photo-Bleaching, Fluorescence Quenching, and Urine Analysis

  • Cheon, Tae-Min;Cheong, Byeong-Seo;Cho, Han-Gook;Kim, Jin-Hee;Kim, Kyoug-Soo
    • Journal of the Korean Chemical Society
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    • v.56 no.5
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    • pp.577-582
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    • 2012
  • Quinine quantitative analysis, photo-bleaching, fluorescence quenching, and urine analysis have been performed by means of a UV-LED fluorometer, which can be easily built and used in a high-school laboratory. The quinine detection range is estimated to be 0.05-80 ppm, enough for many classroom luminescence experiments. The quinine content in commercial tonic water is determined from the calibration curve, and UV photo-bleaching of this anti-malarial drug is demonstrated with clear wavelength dependence. Halide quenching of quinine fluorescence is also observed and the increase in quenching efficiency in the order of $Cl^-$, $Br^-$, and $I^-$ is evident. Urine analyses for the student volunteers have been carried out and the results clearly reveal excretion of the ingested quinine. The student participants are exuberant throughout the course of this study and sense the practices resourceful.

Evaluation on the Satisfaction of School Illumination Quality by Applying SERVPERF Model (SERVPERF 모형을 응용한 학교 조명 품질 만족도 평가)

  • Jee, Soon-Duk;Kim, Sung-Ae;Kim, Chae-Bogk
    • Journal of the Korean Institute of Educational Facilities
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    • v.20 no.3
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    • pp.29-39
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    • 2013
  • This study addresses the evaluation on the satisfaction of school illumination quality by applying SERVPERF model after extracting factors affecting school illumination quality. Three types of illumination systems (fluorescence light, general LED light and high color rendition LED light) were tested by students who have used each illumination system. Three factors such as effectiveness, esthetic sense and function were developed for evaluation. Satisfaction evaluation was performed based on applied SERVPERF model by comparing perceived levels. The differences of perceived levels of satisfaction on the illumination systems were analyzed by ANOVA. The results said respondents satisfy only the high color rendition LED light regardless of three factors. Especially, students who experienced high color rendition LED light have strong intention to recommend that illumination system to other schools. They also express their desire to use that system at home. Interestingly, there is not much satisfaction difference between fluorescence light and general LED light.

Fluorescence Micropatterning Based on the Polymeric Photobase Generator Containing Oxime-Urethane Groups by Dansylation

  • Choi, Won San;Kim, Hak Soo;Chae, Kyu Ho
    • Rapid Communication in Photoscience
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    • v.2 no.1
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    • pp.24-27
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    • 2013
  • A polymeric photobase generator containing oxime-urethane groups is applied to a fluorescence micropatterning material. Polymer bearing oxime-urethane groups was prepared by copolymerization of methyl methacrylate with methacryloyloxyethyl benzophenoneoxime urethane (MBU). The reaction of amino groups in the irradiated copolymer film with dansyl chloride (Dns-Cl) was monitored by using UV absorption, IR absorption and fluorescence spectroscopy. The fluorescence spectrum of the Dns-Cl-treated irradiated copolymer film shows a strong fluorescence with a fluorescence maximum wavelength at 510 nm. A blue fluorescent micropattern with a line width of $2{\sim}3{\mu}m$ was obtained. Treatment of the irradiated copolymer film with Dns-Cl and rhodamine B mixture led to the formation of green, red, and orange-colored fluorescence micropatterns. Thus, various colored micropatterns on a single polymer film can be obtained by selective excitation of each dye molecules.

Development of 20W LED Tube for alternating Fluorescence Luminaires (20W급 형광등 대체형 LED Tube 개발)

  • Kim, Gi-Hoon;Park, Joung-Wook;Kim, Jin-Hong;Cheon, Woo-Young;Song, Sang-Bin
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2009.05a
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    • pp.248-251
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    • 2009
  • 20W 급 LED Tube의 개발을 위하여 LED Package의 광효율이 70lm/W이상을 갖는 Package를 자체 제작하고 이를 적용하여 LED Tube의 광학, 방열 및 기구 구조 설계를 하여 시제품을 제작하였다. 그 결과 시제품의 성능은 광속 1081 lm, 상관색온도 6818 K, Ra 75.71이 성능을 갖는 20W LED Tube를 개발하였다.

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Novel Naphthalene Based Lariat-Type Crown Ethers Using Direct Single Electron Transfer Photochemical Strategy

  • Park, Hea Jung;Sung, Nam Kyung;Kim, Su Rhan;Ahn, So Hyun;Yoon, Ung Chan;Cho, Dae Won;Mariano, Patrick S.
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3681-3689
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    • 2013
  • This study explored a direct SET-photochemical strategy to construct a new family of thioene conjugated-naphthalamide fluorophore based lariat-crown ethers which show strong binding properties towards heavy metal ions. Irradiations of designed nitrogen branched (trimethylsilyl)methylthio-terminated polyethylenoxy-tethered naphthalimides in acidic methanol solutions have led to highly efficient photocyclization reactions to generate naphthalamide based lariat type thiadiazacrown ethers directly in chemo- and regio-selective manners which undergo very facile secondary dehydration reactions during separation processes to produce their corresponding amidoenethio ether cyclic products tethered with electron donating diethyleneoxy- and diethyenethio-side arm chains. Fluorescence and metal cation binding properties of the lariat type enamidothio products were examined. The photocyclized amidoenethio products, thioene conjugated naphthalamide fluorophore containing lariat-thiadiazacrowns exhibited strong fluorescence emissions in region of 330-450 nm along with intramolecular exciplex emissions in region of 450-560 nm with their maxima at 508 nm. Divalent cation $Hg^{2+}$ and $Pb^{2+}$ showed strong binding to sulfur atom(s) in side arm chain and atoms in enethiadiazacrown ether rings which led to significant enhancement of fluorescence from its chromophore singlet excited state and concomitant quenching of exciplex emission. The dual fluorescence emission responses towards divalent cations might provide a new guide for design and development of fluorescence sensors for detecting those metals.

Recent Developments in Correlative Super-Resolution Fluorescence Microscopy and Electron Microscopy

  • Jeong, Dokyung;Kim, Doory
    • Molecules and Cells
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    • v.45 no.1
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    • pp.41-50
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    • 2022
  • The recently developed correlative super-resolution fluorescence microscopy (SRM) and electron microscopy (EM) is a hybrid technique that simultaneously obtains the spatial locations of specific molecules with SRM and the context of the cellular ultrastructure by EM. Although the combination of SRM and EM remains challenging owing to the incompatibility of samples prepared for these techniques, the increasing research attention on these methods has led to drastic improvements in their performances and resulted in wide applications. Here, we review the development of correlative SRM and EM (sCLEM) with a focus on the correlation of EM with different SRM techniques. We discuss the limitations of the integration of these two microscopy techniques and how these challenges can be addressed to improve the quality of correlative images. Finally, we address possible future improvements and advances in the continued development and wide application of sCLEM approaches.

Optical Imaging Technology for Real-time Tumor Monitoring

  • Shin, Yoo-kyoung;Eom, Joo Beom
    • Medical Lasers
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    • v.10 no.3
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    • pp.123-131
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    • 2021
  • Optical imaging modalities with properties of real-time, non-invasive, in vivo, and high resolution for image-guided surgery have been widely studied. In this review, we introduce two optical imaging systems, that could be the core of image-guided surgery and introduce the system configuration, implementation, and operation methods. First, we introduce the optical coherence tomography (OCT) system implemented by our research group. This system is implemented based on a swept-source, and the system has an axial resolution of 11 ㎛ and a lateral resolution of 22 ㎛. Second, we introduce a fluorescence imaging system. The fluorescence imaging system was implemented based on the absorption and fluorescence wavelength of indocyanine green (ICG), with a light-emitting diode (LED) light source. To confirm the performance of the two imaging systems, human malignant melanoma cells were injected into BALB/c nude mice to create a xenograft model and using this, OCT images of cancer and pathological slide images were compared. In addition, in a mouse model, an intravenous injection of indocyanine green was used with a fluorescence imaging system to detect real-time images moving along blood vessels and to detect sentinel lymph nodes, which could be very important for cancer staging. Finally, polarization-sensitive OCT to find the boundaries of cancer in real-time and real-time image-guided surgery using a developed contrast agent and fluorescence imaging system were introduced.