There was intraspecific variation in Echinochloa crus-galli var, crus-galli in response to quinclorac, showing that plan height and dry weight of a locally collected barnyardgrass(Chinjupi) from Chinju were 90.5 and 37.8% of the untreated control, while those of a locally collected one(Iripi) from Iri showed 19.1 and 14.4%, respectively. The normal distribution curve was obtained from frequency distribution of 89 rice cultivars as affected by the application rates of quinclorac at 30, 300, and 3,000g ai/ha. Protein patterns(SDS-PAGE) of two barnyardgrasses belonging to E, crus-galli var. crus-galli such as Iripi and Chinjupi were not affected by the quinclorac application, indicating that inhibition of enzyme and/or protein biosynthesis seems to be not the primary action target of quinclorac. Electronmicroscopic observation on the injured leaf of Iripi which is considered as a susceptible one showed prominent membrane disruption. Chuchungbyeo(rice variety) resulted in a greater inhibition of tomato growth than those from Chinjupi or Iripi, indicating a great amount of quinclorac discharged from rice root, Chinjupi which is relatively tolerant to quinclorac than Iripi, discharged more quinclorac causing a greater inhibition of tomato growth.
Kim, Sang-Su;Park, Jae-Eup;Kim, Ye-Jin;Lee, Yong-Hwan;Lee, In-Yong;Lee, Jeongran;Moon, Byeng-Chul;Ihm, Yang-Bin
Weed & Turfgrass Science
/
v.5
no.4
/
pp.187-190
/
2016
This study was conducted to determine phytotoxicity of domestic rice varieties and characteristic of sensitive gene involved in herbicide reaction of 4-HPPD (4-hydroxy phenylpyruvate dioxygenase) inhibiting herbicides. The five rice varieties were grown for 2 to 3 leaf stage on seedling trays and then transplanted into plastic pot: 4-Japonica type (Sangjubyeo, Sambaekbyeo, Sanduljinmi and Kumyoung) varieties and 1-Indica type variety (IR8). We trialled standard (14 g a.i. $10a^{-1}$) and double fold (28 g a.i. $10a^{-1}$) dose of benzobicyclon treatment at 10 days after transplanting in order to investigate phytoxicity. The Japonica-type Sangjubyeo showed no rice injury but Indica-type IR8 show 4-5 (standard) and 5-6 (double fold) phytoxicity levels. In spite of Japonica-type, Sanduljinmi and kumyoung showed 3-4 and 4-5 levels. Target resistant gene, Hydroxyphenylpyruvate dioxygenase inhibitor sensitive gene No.1 (HIS1) have been isolated from five domestic rice cultivars (Sangjubyeo, Sambaekbyeo, Sanduljinmi, Kumyoung and IR8). Results of the sequence through PCR, all five tested rice cultivars had HIS1 gene regardless to rice cultivars. And the difference between rice varieties from sequence of HIS1 were identified some variation in genes.
Kim, Dong-Kwan;Son, Dong-Mo;Chon, Sang-Uk;Lee, Kyung-Dong;Rim, Yo-Sup
Korean Journal of Plant Resources
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v.22
no.2
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pp.128-135
/
2009
In this study, the selected 789 lines having agronomic values out of over 2,500 mungbean (Vigna radiata (L.) Wilczek) germplasms, examined the 27 characters including hypocotyl color and leaf size of 401 lines in 2005 and of 388 lines in 2006, and analyzed the contents of vitexin and isovitexin in these mungbean germplasms. The average contents of vitexin and isovitexin in mungbean of the 401 lines used in 2005 were 8.71($1.1{\sim}13.4$) and 9.54($0.9{\sim}15.9$) mg/g, respectively, and the correlation coefficiency ($R^2$) of the contents of vitexin and isovitexin was 0.958. Nine lines including VC3890B were selected to be the ones containing high vitexin and isovitexin. The average contents of vitexin and isovitexin in mungbean of the 388 lines used in 2006 were 10.17($2.0{\sim}15.9$) and 10.64($0.2{\sim}17.6$) mg/g, respectively, and the correlation coefficiency ($R^2$) of the contents of vitexin and isovitexin was 0.958. Six lines including VC4096-2B-4-B-2-B were selected to be the ones containing high vitexin and isovitexin. The contents of vitexin and isovitexin were higher in the mungbean with larger leaves and longer ripening period out of the main characters.
Hwang, Yoon-Jung;Yan, Wang;Kim, Won Hee;Lim, Ki-Byung;Kang, Yun-Im
FLOWER RESEARCH JOURNAL
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v.26
no.4
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pp.195-201
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2018
The plant morphological and chromosome characteristics of 'Bonanza', 'Coral Candy' and 'Purple Crystal', a formolongi-Asiatic (FA) interspecific hybrid species bred at the National Institute of Horticultural Science, Rural Development Administration (RDA), were investigated in this study. The flowering time of these species were found to have some variation. 'Bonanza' flowers in the middle to late June (medium-late maturing cultivar), 'Coral Candy' in the mid of June (medium maturing cultivar), and 'Purple Crystal' was observed to be in early June (early maturing cultivar). The flowering direction of all three cultivars are upward facing flowers and having a weak fragrance. The height of the plants was recorded in the range between 101.0 cm ('Purple Crystal') to 142.3 cm ('Bonanza'), thus they are able to develop cut flowers with excellent stem elongation. Flower diameters of 'Bonanza' (17.1 cm) and 'Coral Candy' (16.9 cm) were classified to be large sized flowers. On the other hand, 'Purple Crystal' had a narrow flower diameter (12.3 cm) with an outer petal width of more than 4.0 cm. Leaf length was observed for 'Bonanza' (15.7 cm), 'Coral Candy' (19.7 cm), and 'Purple Crystal' (11.1 cm). Chromosome analysis was done using FISH technique. Results revealed that all three cultivars were observed as triploids (2n=3x=36). FISH analysis also showed 5S/45S rDNA of 'Bonanza', 'Coral Candy' and 'Purple Crystal' as 4/11 loci, 4/12 loci, and 4/11 loci, respectively. The results of the FISH analysis are useful as markers to distinguish cultivars, since the patterns of rDNA observed on the remaining chromosomes are significantly different except FISH patterns of chromosome #3.
The plant "Hong-do-go-deul-ppae-gi" has been considered as Crepidiastrum × muratagenii, a hybrid between C. denticulatum and C. lanceolatum, based on its morphological traits and geographical distribution. To reveal the hybrid origin of Hong-do-go-deul-ppae-gi, we examined additional morphological traits of this plant and its putative parents (C. denticulatum, C. lanceolatum, C. platyphyllum) and analyzed one nuclear ribosomal internal transcribed spacer (ITS) region and four chloroplast regions (trnT-L, trnL-F, rpl16 intron, and rps16 intron). As a result of examining the morphological traits, putative hybrid individuals were classified into three types based on the habit, cauline leaf, outer phyllary, and achene beak traits. A molecular analysis found that the ITS sequences of Type 1 and Type 2 individuals showed additive species-specific sites of C. denticulatum and C. lanceolatum. Plastid sequences of Type 1 and Type 2 individuals showed C. denticulatum and C. lanceolatum sequences, respectively. However, Type 3 individuals had ITS and plastid sequences corresponding to C. denticulatum. Accordingly, Type 1 and Type 2 individuals not only share morphological traits with C. denticulatum and C. lanceolatum but also show additive species-specific sites for C. denticulatum and C. lanceolatum, and not C. platyphyllum, supporting its origin as a hybrid between C. denticulatum and C. lanceolatum. Type 3 had morphological traits similar to other hybrid types but was distinguished with respect to outer phyllaries and demonstrated some resemblance to C. denticulatum. In a molecular analysis, Type 3 was found to be identical with regard to the sequence of C. denticulatum and was judged to be an ecological variation of C. denticulatum.
Proceedings of the Korean Society of Crop Science Conference
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2022.10a
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pp.84-84
/
2022
The limit of crop cultivation is moving northward due to the temperature rise by climate change. There is a problem with crop growth if early sowing is performed at a time when the temperature is low. It is difficult to secure crop productivity and cultivation stability due to the low temperature and short cultivation period. Therefore, this study was conducted to analyze the change in growth characteristics and yield of corn for grain when early sowing is performed in central region of South Korea. This experiment was conducted at experimental field of Suwon in 2021. Three varieties of corn for grain such as Kwangpyeongok, Sinhwangok, and Hwangdaok were sown at intervals of 5 days from 20 March to 15 April. The planting density at this time was sown with a row interval of 70 cm and a plant interval of 25 cm. Nitrogen, phosphoric acid, and potassium fertilizers were applied at 17.4 kg, 3.0 kg, and 6.9 kg per 10a, respectively. Phosphoric acid and potassium fertilizers were all applied before sowing and nitrogen fertilizer was applied 50% before sowing and 50% in the fifth leaf period. The corn growth characteristics and yield components were investigated. The seedling establishment rate by sowing date was in the range of 68.5~88.5%, and it showed a difference depending on the variety. The range of days from sowing to tassel and silk emergence by sowing date was 79.9~98.4 and 81.0~98.9 days, respectively. As the sowing date was delayed, the days from sowing to tassel and silk emergence decreased. The growth characteristics and yield of corn by sowing date are as follows. Plant height was the highest at 241.3 cm at the sowing on 25 March, and Stalk diameter was the thickest at 25.6 mm at the sowing on 31 March. The fresh weight per plant was the highest at 728 g at the sowing on 25 March, and the dry weight per plant was the highest at 185 g at the sowing on 31 March. Corn growth characteristics did not show a certain trend depending on the sowing date, and corn growth was more vigorous at the sowing on March 25 and 31 than the others. In the case of ear weight, it was the heaviest with 344 g at the sowing on 25 March, and filled ear length ratio showed a tendency to decrease as the sowing time was delayed. The weight of 100 grains and grain yield per 10a of maize were the highest at 36.0g and 878.7kg/10a, respectively at the sowing on 25 March. Although the growth and yield of corn for grain were good during early sowing in the central region of South Korea, it is necessary to investigate the limit temperature for early sowing of corn by examining the annual variation according to weather conditions.
Ku, Bon-Il;Kang, Shin-Ku;Sang, Wan-Gyu;Choi, Min-Kyu;Lee, Kyu-Jone;Park, Hong-Kyu;Kim, Young-Doo;Kim, Bo-Kyong;Lee, Jeom-Ho
KOREAN JOURNAL OF CROP SCIENCE
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v.58
no.4
/
pp.353-361
/
2013
The time of panicle initiation change by transplanting date, and this change is affected by heading ecotype and seedling age. So we assessed the variations of panicle initiation, spikelet differentiation and heading date affected by transplanting dates, rice cultivars and seedling ages. And we compared the growth durations and meterological factors between chief growth stages. The differences of growth duration from transplanting date to spikelet differentiation by seedling age were 1~3 days in all transplanting of Unkwang, but it increased to 4 days in Hwayeong transplanting on May 1 and June 30, and Nampyeong transplanting on June 30. The growth durations from panicle initiation to heading of Unkwang and Hwayeong increased until transplanting time by May 31, and decreased thereafter. The growth durations of Nampyeong increased in transplanting on May 16 and May 31. In each transplanting, mean temperature of 30 days after heading was highest in early transplanting, but sunshine hours in the period were highest in transplanting on June 30 in Unkwang, in transplanting on June 15 in Hwayeong, and higher in transplanting on May 31 and June 15 in Nampyeong. The growth duration between spikelet differentiation and heading showed variation according to rice cultivars and transplanting date, Those were 22~26 days in Unkwang, 21~27 days in Hwayeong and 21~28 days in Nampyeong.
In this study, vertical distribution patterns of Quercus mongolica Fisch. ex Ledeb. and Q. serrata Murray in Korea were recognized and possibility of introgressive hybridization and gene flow between Q. mongolica and Q. serrata in Mt. Jiri was inferred by flavonoid analyses. The most critical factor on distribution patterns was the altitude in accordance with temperature condition. A zonal distribution was recognized: Quercus mongolica zone in the upper area and Q. serrata zone in the lower area. In Central Korea, the range of vertical distribution of Q. mongolica was above alt. 100m, almost everywhere, whereas that of Q. serrata was from alt. 0 m to alt. 500(-700) m, and the species is rare above that altitude. But in Southern Korea, Q. serrata is found up to above alt. 1,000 m, whereas frequency of Q. mongolica reduces as elevation in decline and the species is rare below alt. 300 m, even though pure stands being formed on higher mountain slope. Altitudinal distribution of the two species, however, overlaps, where the two species occur together. Thirty-seven individuals of Q. mongolica and Q. serrata in Mt. Jiri and other area were examined for leaf flavonoid constituents. Twenty-three flavonoid compounds were isolated and identified; they were glycosylated derivatives of the flavonols kaempferol, quercetin, isorhamnetin, myricetin, and four compounds among the flavonoid compounds were acylated. Kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside and its acylated compounds were major constituents and present in all individuals. Quercus mongolica is distinguished from Q. serrata by the presence of quercetin 3-O-arabinosylglucoside and by high concentration of three acylated compounds, acylated kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside, and by relatively low concentration or lacking of rhamnosyl flavonol compounds. There are intraspecific variations in flavonoid profiles for Q. mongolica and Q. serrata, the flavonoid profiles for individuals of two species in hybrid zone (sympatric zone) tend to be similar to each other, qualitatively and quantitatively. These findings strongly suggest that gene exchange or gene flow occurs through the introgressive hybridization between Q. mongolica and Q. serrata in Mt. Jiri. Therefore, Quercus crispula, occupying morphologically intermediate position between Q. mongolica and Q. serrata, is suspected of being a hybrid taxon of two putative parental species.
This research was conducted to estimate moisture content in cucurbitaceae seedlings, such as cucumber and watermelon, using hyperspectral imagery. Using a hyperspectral image acquisition system, the reflectance of leaf area of cucumber and watermelon seedlings was calculated after providing water stress. Then, moisture content in each seedling was measured by using a dry oven. Finally, using reflectance and moisture content, the moisture content estimation models were developed by PLSR analysis. After developing the estimation models, performance of the cucumber showed 0.73 of $R^2$, 1.45% of RMSE, and 1.58% of RE. Performance of the watermelon showed 0.66 of $R^2$, 1.06% of RMSE, and 1.14% of RE. The model performed slightly better after removing one sample from cucumber seedlings as outlier and unnecessary. Hence, the performance of new model for cucumber seedlings showed 0.79 of $R^2$, 1.10% of RMSE, and 1.20% of RE. The model performance combined with all samples showed 0.67 of $R^2$, 1.26% of RMSE, and 1.36% of RE. The model of cucumber showed better performance than the model of watermelon. This is because variables of cucumber are consisted of widely distributed variation, and it affected the performance. Further, accuracy and precision of the cucumber model were increased when an insignificant sample was eliminated from the dataset. Finally, it is considered that both models can be significantly used to estimate moisture content, as gradients of trend line are almost same and intersected. It is considered that the accuracy and precision of the estimating models possibly can be improved, if the models are constructed by using variables with widely distributed variation. The improved models will be utilized as the basis for developing low-priced sensors.
Susceptible trees of Pinus densiflora and resistant trees of Pinus rigida following pine gall midge (Tnecodiplosis japonensis Uchida et Inouye) attack were seasonally compared to examine the variation in needle growth and photosynthetic ability, respiration rate, chlorophyll contents, carotenoid and anthocyanin contents. Also, carotenoid and anthocyanin contents of larvae both from soil and from galled tissue were compared during March and September, respectively. The plantation damaged severely by this insect consisted mostly of 10-to 15-year old P. rigida and P. densiflora. The results obtained in this study were as follows: 1) The length of the infested needles of P. densiflora decreased by 48.1 percent compared with the normal needles, while that of P. rigida did 37.4 percent. 2) All of P. densiflora and P. rigida showed higher photosynthetic ability in normal needles than in infested needles. The maximum photosynthetic ability of P. densiflora was shown in mid-August, while that of P. rigida in mid-October. In contrast to that, respiration rate of infested needles was higher than that of normal needles in both species. The respiration rate of P. rigida was higher than that of P. densiflora. 3) P. rigida had higher total chlorophyll contents than P. densiflora. The total carotenoid contents tents in infested needles were higher than those in normal needles of both species. 4) Total carotenoid contents were generally higher in P. rigida than in P. densiflora during the growing season. The total carotenoid content (0.094mg/g) in larvae from soil was similar to that (0.092mg/g) in larvae from galled tissues. 5) Infested needles of both species showed higher anthocyanin contents than normal needles. Higher anthocyanin contents in galled needles were due primarily to its active formation stimulated by larval attack. Thus, reddish-brown coloration occurred only in galled needles of P. densiflora.
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