• Title/Summary/Keyword: LEAF VARIATION

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Variation of the Regenerated Plantlets from in Vitro Culture of Neoregeria carorinae 'Tricolor' and in Vivo Growth of Regenerated Plantlets (네오레게리아 기내배양시 변이발생과 기외 생육)

  • 정향영;한봉희;신학기;김의영
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.5
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    • pp.273-276
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    • 1995
  • In vitro propagation of Neoregeria carorinae 'Tricolor' was achieved by using immature flowers and lateral buds, and the plantlets from tissue culture were transplanted and cultivated in greenhouse. The picking times of explants to decrease disappearance of stripes, and in vivo the growth and flowering of regenerated plantlets as influenced by in vivo healed nun were investigated. The normal plantlet were obtained at a frequency of 67%, in the culture of immature flowers picked at 4 weeks after flower bud differentiation, while all leaf stripes disappeared in the culture of immature flowers picked 1 and 5 weeks after flower bud differentiation. In vivo growth of plantlet from immature flower buds was better than those from lateral buds, and the flowering of 27.8% showed in the greenhouse culture of plantlet from immature culture, but the plantlets from lateral buds did not flower at all. The plantlets rooted on the medium with 0.5 mg/L IBA were the most favorable in green house culture, and the kinds and concentrations of auxin in vitro did not have any influence on variation of plane cultured in greenhouse.

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Phenotypic Diversity of Shea(Vitellaria Paradoxa C. F. Gaertn.) Populations across Four Agro-Ecological Zones of Cameroon

  • Nafan, Diarrassouba;Divine, Bup Nde;Cesar, Kapseu;Christophe, Kouame;Abdourahamane, Sangare
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.223-230
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    • 2007
  • Vitellaria paradoxa commonly called shea is an important agro forestry and fruit-bearing species present in four agro-ecological zones of Cameroon. The goal of this work was the morphological characterization of certain populations of V. paradoxa which will serve as a necessary step for a subsequent genetic study of the species. Morphological observations related to 20 agronomic traits, studied on 8-13 trees selected from each of the eight shea populations across four different agro-ecological zones located in four provinces of Cameroon were studied. The study showed that there was a variation between the populations, related to the traits measured on the trunk, fruit, nut, and leaf. Three shapes of the tree(ball, broom, and trained), five shapes of the fruit(round, oblong, reversed pear, ovoid, and oblong), three colors of the nut(clear brown, dark brown, and blackish brown) were identified. The principal component analysis(PCA) carried out on the quantitative characters revealed 72% of the total variance expressed on the first and second main axis. This variation was essentially explained by the traits measured on the fruits and on the nuts. The analyses showed that only the traits of the fruits and the nuts were discriminative. The shea populations studied were structured into two distinct groups using these discriminative traits.

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Evaluation of Groundwater Recharge using a Distributed Water Balance Model (WetSpass-M model) for the Sapgyo-cheon Upstream Basin (분포형 물수지 모델(WetSpass-M)을 이용한 삽교천 상류 유역에서의 월별 지하수 함양량 산정)

  • An, Hyowon;Ha, Kyoochul
    • Journal of Soil and Groundwater Environment
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    • v.26 no.6
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    • pp.47-64
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    • 2021
  • In this study, the annual and monthly groundwater recharge for the Sapgyo-cheon upstream basin in Chungnam Province was evaluated by water balance analysis utilizing WetSpass-M model. The modeling input data such as topography, climate parameters, LAI (Leaf Area Index), land use, and soil characteristics were established using ArcGIS, QGIS, and Python programs. The results showed that the annual average groundwater recharge in 2001 - 2020 was 251 mm, while the monthly groundwater recharge significantly varied over time, fluctuating between 1 and 47 mm. The variation was high in summer, and relatively low in winter. Variation in groundwater recharge was the largest in July in which precipitation was heavily concentrated, and the variation was closely associated with several factors including the total amount of precipitation, the number of days of the precipitation, and the daily average precipitation. This suggests the extent of groundwater recharge is greatly influenced not only by quantity of precipitation but also the precipitation pattern. Since climate condition has a profound effect on the monthly groundwater recharge, evaluation of monthly groundwater recharge need to be carried out by considering both seasonal and regional variability for better groundwater usage and management. In addition, the mathematical tools for groundwater recharge analysis need to be improved for more accurate prediction of groundwater recharge.

Multivariate Analysis of Agronomic Characteristics of Wheat (Triticum spp.) Germplasm

  • Pilmo Sung;Mesfin Haile Kebede;Seung-Bum Lee;Eunae Yoo;Gyu-Taek Cho;Nayoung Ro
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.303-303
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    • 2022
  • The purpose of this study was to evaluate agronomic characteristics and identify the useful traits to utilize the wheat genetic resources for breeding programs by understanding the phenotypic variation among germplasm through multivariate analysis. In this study, a total of 394 wheat accessions were characterized for 15 agronomic traits using the National Agrobiodiversity Center (NAC) descriptor list, of which 31 accessions from 6 species and 363 unidentified accession (Triticum spp.) available at the NAC, Rural Development Administration (RDA), Korea. Growth characteristics such as leaf width, culm length, spike length, spikelet length, solid stemmed, days to heading, days to maturity, grain-filing period, and also seed characteristics such as width, height, area, perimeter, circle, solidity, and germination percent were studied. Among the 15 agronomic characteristics, the germination percent showed the smallest variation between resources (CV = 0.4%), and the spikelet length (CV = 66.5%) showed the highest variation. A strong positive correlation was found between seed traits such as seed height and seed area (r = 0.90), seed height and seed perimeter (r = 0.87) and seed length and width (r = 0.80). Principal component analysis (PCA) was conducted and the first five principal components comprised 76.7% of the total variance. Among the first five PCs, PCI accounted for 28.5% and PC2 for 20.0%. Wheat resources (394) were classified into four clusters based on cluster analysis, consisting of 215 resources(I), 117 resources(II), 48 resources(III), and 14 resources(IV). Among the clusters, the resources belonging to Cluster III showed the lowest seed width, height, area, and perimeter characteristics compared to other clusters. The wheat resources belonging to cluster IV had small seed width and low germination percent, but took longer to form heads and mature than resources in other clusters. These results will serve as the basis for further genetic diversity studies, and important agronomic characteristics will be used for improving wheat, including developing high-yielding and resistant varieties to biotic and abiotic stresses via breeding programs.

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Leaf variants of Pinus and their ITS DNA sequences (소나무속 잎 변이와 그의 ITS DNA 염기서열)

  • Koo, JaChoon;Whang, Sung Soo
    • Korean Journal of Plant Taxonomy
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    • v.43 no.1
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    • pp.63-68
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    • 2013
  • ITS DNA sequences of two variants of Pinus spp. having single fasciculate leaf or two to three fasciculate leaves within an individual were analysed in order to determine their origin. Also, the same DNA locus of P. densiflora, P. rigida and P. koraiensis, distributed at the same region together with the OTUs having leaf variations, were analysed to compare with each other. Aligned sequences including ITS1, 5.8S and ITS2 were 580~584 bp in length. The 5.8S region was well conserved among all the OTUs we tested except for P. koraiensis, which has two nucleotide substitutions. The partial ITS1 region upstream of the 5.8S region showed relatively high sequence diversity compare to the ITS2 and has 181~185 bp in length. In this region, the sequences of the two variants were identical to that of P. densiflora. The ITS2 has identical for all OTUs in length and the two variants also have same sequences compare to that of P. densiflora. These two variants and samples of P. densiflora were grouped together in the UPGMA tree with 100% similarity level. The result strongly suggest that these two variants were originated from P. densiflora.

Variation of Image Quality and Dose by Applying Multi-Leaf Collimator for Digital Mammography (디지털 유방촬영장치에서 다엽 조리개 적용으로 인한 화질과 선량의 변화)

  • Kwon, Soon Mu;Kim, Boo Soon;Park, Hyung Jun;Kang, Yeong Han
    • Journal of the Korean Society of Radiology
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    • v.9 no.7
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    • pp.535-540
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    • 2015
  • Collimator has important functions with control primary X-ray that decrease radiation exposure dose for patients and reduce scatter ray and make better quality of image. But there are no regulations for X-ray mammography device of collimator, so widely used device adopt rectangularly controlled collimator. Though digital X-ray mammography device expand supply recently, rectangularly controlled collimator of film/screen mode still used. After searching for real condition of beam field with digital mammography, we made a multi-leaf collimator which is able to adjust the beam field in accordance with size and shape of breast, and we measuring up the transitions of image quality, average glandular dose(AGD) and, Dose area product(DAP). There are no significant differences between rectangularly controlled collimator and multi-leaf collimator, and DAP value decreased by 50.72%. As conclusion, there needs to expand the use of multi-leaf collimator for optimum adoption of beam field in digital mammography, and also need to develop an automatic regulation of beam field for reduce of exposure dose to patients.

Monitoring of Rice Growth by RADARSAT and Landsat TM data (RADARSAT과 Landsat TM자료를 이용한 벼 생육모니터링)

  • Hong Suk-Young;Rim Sang-Kyu
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.2 no.1
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    • pp.9-15
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    • 2000
  • The objective of this study is to evaluate the use of RADARSAT and Landsat TM data for the monitoring of rice growth. The relationships between backscatter coefficients($\sigma$$^{0}$ ) of RADARSAT data and digital numbers (DN) of Landsat TM and rice growth parameters were investigated. Radar backscatter coefficients were calculated by calibration process and then compared with rice growth parameters; plant height, leaf area index (LAI), and fresh and dry biomass. When radar backscatter coefficient ($\sigma$$^{0}$ ) of rice was expressed as a function of time, it is shown that the increasing trend ranged from -22--20dB to -9--8dB as growth advances. The temporal variation of backscatter coefficient was significant to interpret rice growth. According to the relationship between leaf area index and backscatter coefficient, backscatter coefficient underestimated leaf area index at the beginning of life history and overestimated, at the reproductive stage. The same increasing trend between biomass and backscatter coefficient was shown. From these results, RADARSAT data appear positive to the monitoring of rice growth. Each band of time-series Landsat TM data had a significant trend as a rice crop grows during its life cycle. Spectral indices, NDVI[(TM4-TM3)/(TM4+TM3)] and RVI(TM4/TM2), derived from Landsat TM equivalent bands had the same trend as leaf area index.

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Inhibition of Floral Induction and Variation of Yield in Angelica gigas Nagai (참당귀의 화성억제와 수량변화)

  • 조선행;김기준
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.151-158
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    • 1993
  • Since the inhibition of floral induction was considered to be an important subject to get high yield of Angelica gigas, the effects of low temperature, daylengh and shading were investigated in this study. The yield of A. gigas root grown by inhibition of floral induction was compared with the yield obtained by traditional cultivation method. When the seedings were exposed to the natural low air temperature condition until late of November, the plants which had 6 to 8 leaves showed vernalizing effect, and when the low temperature treatment was prolonged until mid of January, 4 to 5 leaf plants showed vernalizing effect. But 2 to 3 leaf plants did not show vernalizing effect regardless of the length of chilling treatment. The effectiveness of exposure to low temperature was markedly dependent upon the age of plants. In the artificial low temperature treatment the range of temperature varied with plant age. Vernalizing effect at 1,5 and 9$^{\circ}C$ of temperature did not show 3,5,6 leaf plants, respectively. The bolting percent in 8 leaf plant at 1,5 and 9$^{\circ}C$ for 8 weeks decreased by 80,45 and 5%, respectively. As daylength became longer, the bolting percent of A. gigas was increased. plants which were grown in the full sunlight showed the highest value in bolting percent, and the bolting percent was not decreased significantly with changing from full sunlight to 50% shading. When A. gigas were grown by the inhibition of floral induction, dry root weight per l0a showed the greatest value in 3 year old plant which 3.6 and 2.4 times higher than that of 1 and 2 year old plant, respectively. The yield of root was increased by 73.7% in 2 year old plant and 159.6% in 3 year old plant compared with the yield obtained by traditional cultivation method.

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TFWT and OBT Concentrations in Soybean Plants Exposed to HTO Vapor at Different Growth Stages (콩의 생육단계별 HTO 증기 피폭에 따른 작물체내 TFWT 및 OBT 농도)

  • Lim, K.M.;Choi, Y.H.;Lee, W.Y.;Park, H.G.;Kang, H.S.;Choi, H.J.;Lee, H.S.
    • Journal of Radiation Protection and Research
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    • v.29 no.4
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    • pp.213-219
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    • 2004
  • Soybean plants were exposed to HTO vapor in an exposure box for 1 hour at different growth stages. Relative concentrations of TFWT at the end of exposure (percent ratios of TFWT concentrations to mean HTO concentrations in air moisture in the box during exposure) decreased on the whole in the order of leaf > shell > seed > stem with the highest values of 40.2% and 6.4% for leaf and stem, respectively. TFWT concentrations reduced by factors of several thousands to several hundred-thousands from the end of exposure till the harvest. The reduction factor decreased in the order of leaf > shell > seed > stem. Relative OBT concentrations at harvest (ratios of the OBT concentration in the dry plant part at harvest to the initial leaf TFWT concentration, ml $g^{-1}$) were in the range of $2.2{\times}10^{-5}{\sim}9.5{\times}10^{-3}$ for seeds being the highest when the exposure was performed at the actively seed-developing stage. The exposure time-dependent variation in the OBT concentration was much greater in seeds and shells than in leaves and stems. It was indicated that OBT would contribute to almost all the radiation dose due to the consumption of soybean seeds in most cases after an acute exposure of growing plants to HTO vapor. Present results are applicable to establishing and validating soybean $^3H$ models for an acute accidental release of HTO.

A taxonomic study of the genus Hosta in Korea (한국산 비비추속(Hosta Tratt.) 식물의 분류학적 연구)

  • Jo, Hyun;Kim, Muyeol
    • Korean Journal of Plant Taxonomy
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    • v.47 no.1
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    • pp.27-45
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    • 2017
  • A taxonomic study of the genus Hosta (Lilliaceae) in Korea was conducted based on morphological characteristics. Hosta clausa varieties share several characteristics (rhizomes with underground stolons, smooth scapes, and purple anthers). Hosta clausa var. normalis has an open perianth, long pistils, and fertile fruit. H. clausa var. clausa can be distinguished from H. clausa var. normalis by its closed perianth and sterile fruit. H. clausa var. geumgangensis has sterile fruit, green petioles, short pistils, and short narrow perianth tubes. H. clausa var. ensata has very narrow, sword-shaped leaves. It can be included in H. clausa var. normalis, which has a large degree of variation in leaf and petiole shape. H. jonesii has smooth scapes, yellow anthers, and bell-shaped flowers. It can be distinguished from the related species H. tsushimensis found on the Tushima islands in Japan by its wide perianth tube. H. yingeri is distinguished by its funnel-shaped flowers, 3 long and 3 short stamens, and smooth scape; the related species H. laevigata has 6 equally-sized stamens, narrowly lanceolate leaves, and wavy leaf margins. Because H. laevigata is not found in its type locality Isl. Heugsando, its taxonomic position must be determined at a later time. H. minor shares several characteristics (scapes with ridges and yellow-colored anthers) with the related taxa H. venusta. However, H. venusta is changed to H. minor var. venusta because it has overlapping characteristics in plant size, number of flowers, leaf size, and leaf shape. Consequently, the genus Hosta in Korea is classified into 5 species, 3 varieties, and 6 forms.