• 대한한의학회지
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• 제22권4호
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• pp.90-100
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• 2001

Objectives : Bambusae Caulis in Liquamen (BCL) has been used for stamina fortification in Oriental Medicine for thousand years. The goal of the present study was to investigate the muscle antifatigue effect of BCL. Methods : The mice were divided into three groups. The control group was allowed to swim for 10 minutes without BCL infusion. For the preventive effect of BCL, another group referred to BCL-1 was fed with BCL an hour before the swimming exercise. BCL-2 group for the reversion effect was forced to swim for 10 minutes at first, then fed with BCL followed by half an hour rest and another 10 minute swimming exercise. All the serum samples collected by heart puncture were used for the measuring biochemical factors such as LDH, CPK, glucose, lactate, triglycerides and total cholesterol. Results : In the BCL-2 group, lactate and LDH were significantly reduced compared to the control. BCL infusion during the exercise was effective in metabolizing LDH, reducing the conversion ratio of pyruvate into lactate. In the BCL-1 group, it was not effective in antifatigue function. The level of glucose was significantly reduced in BCL-1 group compared to the control. When the BCL was infused before the exercise, it is assumed that glycogenolysis seen during the exercise was prevented. When the BCL was infused during the exercise, the triglycerides and total cholesterol were increased significantly compared to the control. BCL seems to cause the increase of lipolysis to utilize the fat as an energy source. Unlike other parameters, CPK did not show any changes from BCL infusion. Conclusions : Based on the above results, BCL is found to be involved in energy metabolism. Further studies are needed to find out the underlying mechanism of BCL for its effect on stamina fortification.

• Korean Journal of Acupuncture
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• 제23권2호
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• pp.155-166
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• 2006

Objectives: This study was undertaken to determine if Plantaginis Semen(PS) has a protective effect against $CCI_4$ induced hepatoxicity in Rats. Methods: The experimental groups were divided into Normal group (untreated Group), Control group $(group\;treated\;with\;CCI_4)$, Sample 1 (group treated with 0.1 ml Plantaginis Semen herbal acupuncture in both sides of $G\bar{a}nsh\bar{u}\;(BL_{18})$ after $CCI_4$ treating) and Sample 2 (group treated with feeding of 1 cc Plantaginis Semen extraction after $CCI_4$ treating). Rats were administered orally $CCI_4\;(0.1\;ml/kg)$ for 4 days. In experiments for PS effect, rats of Sample 1 group received 0.lml herbal acupuncture of PS extraction in both sides of corresponding $G\bar{a}nsh\bar{u}\;(BL_{18})$ of human body for 3 days after treated $CCI_4$. Rats of Sample 2 group feeded PS extraction (1cc) for 3 days after treated $CCI_4$. Variation of weight and biochemical assays (AST, ALT, LDH, ALP, total cholesterol, triglyceride, total protein, albumin) were performed. Results: In Control group, $CCI_4$ increased serum AST, ALT, LDH, ALP, total protein, albumin and decreased weight, total cholesterol, triglyceride. Sample 1 and Sample 2 significantly decreased serum AST, ALT, LDH, ALP, total protein, albumin as compared with Control group. Conclusion: These results indicate that PS could be used in prevention and treatment of hepatoxicity. However, precise mechanisms of PS protection remain to be determined.

• 동아시아식생활학회지
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• 제15권1호
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• pp.57-64
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• 2005

The effects of water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower on serum lipid concentrations were evaluated in rats. Forty-eight male Sprague-Dawley rats weighing l00±l0 g were divided into six groups and fed high fat diets for six weeks. Experimental groups were administered with following diets; Control diet, animal, plant high fat diet and control and high fat diets with 2% water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower. Tissue weights of liver, lung, stomach, heart, kidney and spleen of high fat diet exposed rats were reduced by water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups. The concentrations of serum triglyceride in rats fed the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower were lower than those in other groups. The concentrations of total cholesterol in water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower group were lower than those in high fat diet groups. The concentrations of HDL-cholesterol in serum of the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups were tended to be lower than those of other groups. GPT and GOT activities were decreased in water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups and than in the high fat group. LDH activity was lower in the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups than in the high fat group. These results suggest that water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

• 한국식품영양학회지
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• 제11권3호
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• pp.312-318
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• 1998

도라지의 급이가 고콜레스테롤혈증 흰쥐의 간기능과 지질대사에 미치는 영향 구명하기 위하여 SD계 숫흰쥐에게 동결건조한 도라지 분말 5%와 도라지 분말 5%에 해당하는 식물섬유, saponin, 에탄올 추출물을 각각 급이하여 3주간 실험 사육한 후 혈청효소 및 간장의 지질성분 분석, 간세포의 형태를 검토하였다. 혈청 중 AST, ALT효소활성은 대조군에 비해 전 실험군이 유의적으로 감소하였고 그 중 saponin 급이군이 가장 낮았고 그 다음이 식물 섬유군이었다. LDH효소 활성도 전 실험군에서 유의적으로 낮게 나타났으며 22년근 도라지의 식물 섬유군과 saponin군이 낮은 값을 보였다. 간장 중의 총 콜레스테롤 함량은 4년근과 22년근 도라지 분말 및 식물섬유 급이군이 비교적 낮았으며, 중성지질 함량은 대조군이 비해 전 실험군에서 낮았으나, 각 실험군간의 차이는 크지 않았고, 인지직 함량은 전 실험군에 있어 비슷한 수준이었다. 간장 조직세포의 전자현미경 관철 결과, 대조군은 지방구가 증가되어 있는 반면 도라지 급이군에서는 감소되었으며, 22년근 도라지 급이군은 간장 글리코겐 축적량이 증가되어 있었다.

• 셀메드
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• 제6권2호
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• pp.12.1-12.7
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• 2016

Cocos nucifera (C. nucifera) oil is indigenously used to treat cardiovascular diseases. However, coconut husk fibre (which is rich in polyphenols) has not been screened for this property. Based on the ethnomedicinal use of polyphenols in treating cardiovascular diseases, this study was carried out to evaluate the effects of polyphenols of C. nucifera husk fibre on selected cardiovascular disease indices in mice. Fifty adult male Swiss albino mice were assigned randomly into five groups (A-E). Mice in groups B, C, D and E were administered 31.25, 62.5, 125, and 250 mg/kg body weight polyphenols of ethyl acetate extract of C. nucifera husk fibre respectively while the control group (A) mice received 5% DMSO for seven days. The mice were sacrificed twenty four hours after the last administration of polyphenols. Heart and plasma lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities and plasma lipid profile were determined. Results revealed significant reduction (^*p< 0.05) in plasma levels of total cholesterol and LDL-cholesterol with no significant change (^*p> 0.05) in HDL-cholesterol, triglyceride and VLDL levels in the plasma at all doses of polyphenols administered compared to controls. There was significant reduction (^*p< 0.05) in the activities of heart AST and LDH while plasma ALT, AST, and ALP activities were not significantly altered (^*p> 0.05) at all doses of polyphenols administered compared to controls. These results suggest that the polyphenols of C. nucifera husk fibre possess cardio-protective properties and also indicate their possible use in the treatment of cardiovascular diseases.

• 한국잠사곤충학회지
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• 제43권2호
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• pp.104-108
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• 2001

뽕나무 품종별(대륙뽕, 국상20호, 수봉뽕, 야상오디, 카타네오)오디 메탄을 추출물이 흰쥐의 지질대사 및 간장 기능에 미치는 영향을 알아보기 위하여, 콜레스테롤을 투여하여 고지혈증을 유발시킨 흰쥐에 뽕나무 품종별 오디의 메탄을 추출물을 경구투여한 후, 혈청 중의 콜레스테롤, 중성지질함량과 더불어 GOT, GPT, LDH의 활성을 측정하였다. 그 결과를 요약하면 다음과 같다. 1 냉동건조시킨 오디의 메탄을 추출물 수율은, 대륙뽕 50.9%, 국상20호 62.0%, 수봉뽕 64.6%, 카타네오 49.8% 및 야생오디 43.5%로 수봉뽕 품종이 가장 높았고, 품종간 차이가 비교적 컸다. 2. 야상오디 메탄을 추출물 처리군은 중성지질함량을 감소시켜 정상의 85% 수준까지 회복되었고, 혈청 중 총 콜레스테를 함량은 국상20호 >카타네오 > 야삼오디 >수봉뽕 오디 메탄을 추출물 처리군 순으로 감소되었으나, 그 효과가 유의적이지는 않았다. 3.간장 기능에 미치는 효과를 알아보기 위하여 혈청 중의 GOT, GPT및 LDH활성을 측정한 결과, 야상오디 메탄을 추출물 처리군은 GOT치를 감소시켜 정상의 86% 수준으로 회복시켰고, 국상20호는 68%, 수봉뽕은 66%, 카타네오는 50% 수준으로 GOT치를 감소시켰다.

• 동아시아식생활학회지
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• 제10권4호
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• pp.305-310
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• 2000

This study was attempted to investigate the effect of pine pollen on the chloroform toxicity in rat serum and liver. The subjects were administrated with the graded concentraton of tow different levels of chloroform(1%, 5%) and an additional amount of pollen (1%, 5%) to some groups. the results were as follows: The activity of aspartate aminotrasferase(AST), alanine aminotrasferase(ALT) and lactate dehydrogenase(LDH) in serum, liver, and kidney increased in proportion to the chloroform concentration, but decreased in the pine pollen-treated groups. The amount of total cholesterol in serum of he chloroform administration groups was higher than that of the control group, and it decreased gradually with pine pollen administration.

• 약학회지
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• 제32권5호
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• pp.351-361
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• 1988

The herbicide, paraquat, has caused numerous poisonings in man and farm animals since its interoduction. Althought the lungs are the primary sites of toxicological effect, other organs are also damaged, such as liver, heart, and kidney. The biochemical mechanisms responsible for the toxicity of the herbicide are not clearly understood. The purpose of the present study was to determine the hepatotoxity of paraquat in rats and the effects of glycyrrhizin on the paraquat toxicity. The experimental results are the followings. 1. The values of serum AST, ALT, LDH, ALP, glucose, and cholesterol were significantly increased in the paraquat administered group. 2. The TBA values in serum and liver were also increased in the treated group. 3. The biochemical parameters of serum, such as AST, ALT, LDH and TBA values, were significantly low in the paraquat plus glycyrrhizin group in comparison with that of the paraquat only. The above results suggest that the glycyrrhizin, somehow, detoxify the hepa to toxicity of paraquat in the experimenta-animals.

• 대한한의학회지
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• 제26권1호
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• pp.148-160
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• 2005

Objective: We investigated the effects of deer antler extract (DAE) on the expression of the weight, leptin, and serum levels (lipid metabolism) of the obese SD rats induced by a $2\%$ cholesterol diet in vivo. Methods: In vivo, the weight, leptin and serum levels were measured after a period of 7 weeks for obese SD rats induced by a $2\%$ cholesterol diet and treated with DAE of three concentrations (0.938 g/kg/5ml, 1.875 g/kg/5ml, 3.75 g/kg/5ml). Results: 1. Body weight didn't change significantly. 2. Leptin levels significantly decreased (p<0.05). 3. Total cholesterol, triglyceride, LDH, sGOT & sGPT levels significantly decreased in the sample (p<0.05). 4. The HDL cholesterol levels significantly increased in the sample (p<0.05). 5. The average size of the epididymal fat cells significantly decreased as the concentration of deer antler extract increased (p<0.05). Conclusion: Deer antler effects inhibition of obese rats.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2002년도 추계학술대회초록집
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• pp.132-132
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• 2002

Mostly, hypercholesterolemia has been focused on atherosclerosis and coronary heart disease and can be produced by intake of high cholesterol diet. However, toxic effects of cholesterol itself on liver and relationship between intake of high cholesterol diet and hepatic fibrosis have not been clearly investigated. Male Wistar rats were fed diet supplemented with 1.0 ％ cholesterol and 0.3 ％ sodium cholate for 12 weeks. Rats were sacrificed at 0, 3, 6, 9 and 12, respectively. Histopathological and blood chemical studies were performed on these animal sets. Total cholesterol, AST, ALT and LDH levels increased from week 3 and maintained around that level throughout the experiment compared to control. However, TG and albumin levels were the same or lower than those of control. Intake of high cholesterol and sodium cholate diet caused hepatic necrosis, macrophage infiltration, steatosis and fibrosis. Following feeding this diet to rats, hepatic necrosis, macrophage infiltration and steatosis markedly increased throughout the experiment, comparing to control. Collagen deposition and myofibroblasts were detected from at week 9 to 12 in the liver. Mast cell increased in proportion to the degree of hepatic damages. In conclusion, these results suggest that intake of high cholesterol diet is a risk factor on hepatic steatosis and fibrosis as well as atherosclerosis and coronary heart disease. Furthermore, this animal model for hepatic fibrosis can be use for application of anti-fibrogenic agents screening in vivo.