• 대한소아치과학회지
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• 제49권2호
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• pp.197-205
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• 2022

이 연구는 국내에서 불소치약의 농도에 따른 불소함유 수복재의 불소 재방출량의 차이를 알아보기 위하여 시행되었다. 글라스아이오노머(Fuji IX GP EXTRA), 레진강화형 글라스아이오노머(Fuji II LC), alkasite 수복재(Cention N), 복합레진(Filtek^TM Z350XT)의 시편이 제작되었고, 1, 3, 7, 14, 21, 28일에 불소 방출량이 측정되었다. 그 후, 각 수복재에 무불소, 500 ppm, 1450 ppm의 불소치약을 적용하여 불소 재방출량을 1, 3, 7일에 측정하였다. 글라스아이오노머가 측정 7일차 까지는 가장 높은 누적 불소 방출량을 보였고, 14일차부터는 레진강화형 글라스아이오노머가 가장 높은 누적 불소 방출량을 보였으나, 두 수복재의 누적 불소 방출량의 차이는 유의하지 않았다. 500 ppm의 불소치약으로 불소 재충전 시에는 alkasite 수복재만 불소 재방출량의 차이가 유의하였고(p < 0.017), 1450 ppm의 불소치약으로 불소 재충전 시에는 모든 수복재 군이 무불소치약군에 비하여 불소 재방출량이 유의하게 높았다(p < 0.017).

• 대한본초학회지
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• 제34권6호
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• pp.57-62
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• 2019

Objective : Gastritis is a major complication of gastrointestinal disease. Lonicera japonica is used in folk medicine to treat different diseases such as exopathogenic wind-heat, epidemic febrile diseases, sores, carbuncles and some infectious diseases. Therefore, this study examined the effects of Lonicera japonica water extract (LJE) on HCl/ethano-linduced acute gastric ulceration and anti-oxidants properties. Methods : LC-ESI-IT-TOF MS was employed for rapid identification of major compound from LJE. The antioxidant activities were evaluated through total polyphenol and flavonoid contents and radical scavenging assays and superoxide dismutase (SOD)-like activity. SD rats were randomly divided into five different groups including the normal group, ulcer group, positive group (20 kg/mg of omeprazole, ip), and experimental groups (100 kg/mg and 500 kg/mg of LJE, ip). Results : 4,5-Dicaffeoyl quinic acid, loganic acid, secologanic acid, sweroside, loganin, vogeloside were identified based on the detection of the molecular ion with those of literature data. The LJE was possessed free radical scavenging activities such as DPPH (IC₅₀=189.7 ㎍/㎖), ABTS (IC₅₀=164.5 ㎍/㎖), and SOD-like activity (IC₅₀=405.02 ㎍/㎖). Macroscopic and histological analyses showed LJE treated group were significantly reduced to an extent that it allowed leukocytes penetration of the gastric walls compared with the ulcer group. In addition, an ulcer inhibition rate and prostaglandin E₂ levels were increased in rats treated with LJE. Conclusion : The present study has demonstrated the antioxidantive and gastroprotective effect of LJE, these findings suggested that LJE has the potential for use in treatment of gastric disorders.

• Journal of Applied Biological Chemistry
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• 제65권3호
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• pp.203-213
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• 2022

미선나무(Abeliophyllum distichum) 1속 1종의 한반도 고유식물이다. 꽃잎색의 변이에 따라 미선(백색), 분홍미선, 상아미선 그리고 연노랑색의 옥황1호 품종 등으로 구분된다. 본 연구에서는 4종류의 꽃 추출물(FAD)로부터 생리활성물질의 함량과 항염증 활성 차이를 비교·분석하였다. FAD의 생리활성 물질은 LC/MS 분석을 통해 rutin, hirsutrin, 그리고 acteoside를 분석하였다. DPPH와 ABTS 라디칼 소거활성을 통한 항산화 활성을 분석하였으며, 항산화 활성은 각 추출물의 생리활성 물질의 함량에 따라 유의성 있는 활성을 나타내었다. FAD는 LPS로 유도된 RAW 264.7 세포에서 염증 유발 매개체(산화질소, iNOS, COX-2)의 발현을 감소시켰다. 또한 NF-κB와 MAPK 신호전달 경로 조절을 통한 항염증 효과를 확인하였다.

• Journal of Applied Biological Chemistry
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• 제65권3호
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• pp.173-181
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• 2022

본 연구는 케일 중 flubendiamide의 잔류 특성을 조사하여 생산단계 잔류허용기준(PHRL, Pre-Harvest Residue Limit)을 설정하고자 하였으며, 세척용매 및 세척방법에 따른 제거효율을 알아보기 위해 수행되었다. 포장 시험은 포장 1 (경기도 안성시)과 포장 2(경기도 이천시)의 시설재배 하우스 두 곳에서 실시하였다. 농약의 안전사용기준에 따라 10일간격 2회 살포하고 0 (2시간 후), 1, 2, 3, 5, 7, 10일차에 수확하였다. Flubendiamide의 생물학적 반감기는 액체 크로마토그래피와 탠덤 질량분석기(LC-MS/MS)를 결합하여 분석한 시료 중 농약의 소실곡선으로부터 산출되었다. 분석법상의 정량한계(MLOQ)는 0.01 mg/kg 이었으며, 10 MLOQ 및 MRL (0.7 mg/kg) 두 수준에서 회수율 시험을 한 결과 각각 104.2±3.6, 101.9±10.2%의 회수율을 나타냈다. 케일 중 flubendiamide의 감소상수는 포장 1에서 0.2437, 포장 2에서 0.1981이었다. 감소상수를 사용하여 얻은 PHRL 계산식은 다음과 같이 추정되었다: 수확 10일전 잔류농도가 8.0 mg/kg 미만이라면 수확일의 잔류농도는 MRL 수준 이하일 것으로 판단된다. 세척에 의한 flubendiamide의 평균 제거율은 식초(39.8%)로 세척하였을 때 가장 높았고, 베이킹소다(31.7%), 칼슘파우더(30.2%), 중성세제(27.2%), 수돗물(15.9%) 순이었다. 본 연구의 결과는 농민과 소비자 모두에게 안전한 농산물을 생산하거나 소비하는데 유용할 것 이다.

• Journal of Ginseng Research
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• 제46권6호
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• pp.750-758
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• 2022

Background: Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI. Methods: The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LC-MS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a proteineprotein interaction (PPI) network in STRING database and conducted gene ontology (GO) enrichment analysis. Finally, we assessed the effects of ginseng total saponins (GTS) and different ginsenosides on mitochondrial function by measuring the activity of the mitochondrial respiratory chain complex and performing molecular docking. Results: We screened 2526 MCI-related protein targets by databases and 349 ginsenoside-related protein targets by DARTS. On the basis of these 81 overlapping genes, enrichment analysis showed the mitochondria played an important role in GTS-mediated MCI pharmacological process. Mitochondrial function analysis showed GTS, protopanaxatriol (PPT), and Rd increased the activities of complex I in a dose-dependent manner. Molecular docking also predicted the docking pockets between PPT or Rd and mitochondrial respiratory chain complex I. Conclusion: This study indicated that ginsenosides might alleviate MCI by targeting respiratory chain complex I and regulating mitochondrial function, supporting ginseng's therapeutic application in cognitive deficits.

• 한국환경생태학회지
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• 제36권1호
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• pp.30-55
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• 2022

본 연구에서는 경기도 파주시에 위치한 삼봉산 (282m), 자웅산 (263m) 지역을 중심으로 관속식물상 조사를 실시하였다. 2개 산지 식물상 조사결과, 94과 271속 383종 11아종 34변종 6품종으로 총 434분류군의 관속식물이 조사되었다. 분류군별로는 양치식물이 10과 24분류군, 나자식물이 2과 8분류군, 쌍자엽식물이 73과 322분류군, 단자엽식물이 9과 80분류군으로 확인되었다. 주요 식물은 특산식물 키버들, 세잎승마, 개나리, 오동나무, 백운산원추리 5분류군, 산림청 지정 희귀식물은 8분류군이 확인되었다. 취약종(VU)은 세잎승마, 왜박주가리, 금붓꽃이 확인되었고, 약관심종(LC)은 쥐방울덩굴, 금강제비꽃, 두루미천남성, 지치, 말나리가 확인되었다. 침입외래식물은 소리쟁이, 미국자리공, 유럽점나도나물, 끈끈이대나물, 흰명아주, 좀명아주, 일본목련, 개소시랑개비, 족제비싸리, 자주개자리, 아까시나무, 토끼풀, 애기땅빈대, 수박풀, 달맞이꽃 등 총 40분류군으로 조사되어, 조사식물 434분류군의 9.2%를 차지하였다. 이 지역은 취락시설과 가까운 산지들로서 특기할만한 식물들의 생태적인 보전·방안 및 대책수립이 진행되어야 할 것으로 판단된다.

• 한국자원식물학회지
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• 제36권1호
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• pp.32-61
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• 2023

본 연구는 대구·경북 일대 산림습원 지역의 관속식물을 연구하기 위해 수행하였다. 조사는 2020년 6월부터 2021년 10월까지 총 26일에 걸쳐 수행하였고, 확인한 관속식물은 104과 286속 406종 12아종 34변종 6품종 1교잡종 등 총 459분류군으로 정리하였다. 산림청 지정 희귀식물 5분류군: 위기종(EN) 께묵 1분류군, 취약종(VU) 왜박주가리 및 애기물꽈리아재비 2분류군, 약관심종(LC) 꽃창포 1분류군 및 자료부족종(DD) 늦둥굴레1분류군을 확인하였다. 특산식물은 은사시나무, 키버들, 할미밀망, 금오족도리풀 등 11분류군을 확인하였다. 환경부 지정 구계학적특정식물 57분류군: IV등급 긴오이풀 등 3분류군, III등급 박달나무, 쐐기풀 등 8분류군 등을 확인하였다. 귀화식물은 27분류군으로 이 중 생태계교란외래식물은 애기수영, 돼지풀, 미국 쑥부쟁이 등 3분류군을 확인하였으며, 도시화지수는 8.44%, 귀화율은 5.88%로 일반적인 산림의 귀화율인 4%보다 다소 높아 점차 교란이 진행되는 것으로 판단된다. 습지식물은 총 74분류군: 절대습지식물 미나리 등 30분류군, 임의습지식물 털개구리 미나리 등 44분류군을 확인하였다. 산림습원식물은 과거 조사와 비교해볼 때 출현식물은 쇠뜨기(Equisetum arvense L.) 등 277분류군은 공통적으로 출현하였으며, 182분류군은 새롭게 확인하였다. 과거 묵논의 형태로 이루어진 산림습원이 주를 이루고 있어 귀화식물의 유입이 잦아 귀화율이 높아지고 있음을 확인하였다. 따라서 자생식물들의 위협이 될 우려가 있으므로 생태계교란외래식물은 초봄에 일찍 제거하는 것이 바람직하며 이와 더불어 귀화식물은 추가적인 유입을 막을 수 있는 지속적이고 적극적인 관리가 필요할 것으로 판단된다.

• Journal of Ginseng Research
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• 제47권2호
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• pp.302-310
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• 2023

Background: The most common type of dementia, Alzheimer's disease (AD), is marked by the formation of extracellular amyloid beta (Aβ) plaques. The impairments of axons and synapses appear in the process of Aβ plaques formation, and this damage could cause neurodegeneration. We previously reported that non-saponin fraction with rich polysaccharide (NFP) from Korean Red Ginseng (KRG) showed neuroprotective effects in AD. However, precise molecular mechanism of the therapeutic effects of NFP from KRG in AD still remains elusive. Methods: To investigate the therapeutic mechanisms of NFP from KRG on AD, we conducted proteomic analysis for frontal cortex from vehicle-treated wild-type, vehicle-treated 5XFAD mice, and NFP-treated 5XFAD mice by using nano-LC-ESI-MS/MS. Metabolic network analysis was additionally performed as the effects of NFP appeared to be associated with metabolism according to the proteome analysis. Results: Starting from 5,470 proteins, 2,636 proteins were selected for hierarchical clustering analysis, and finally 111 proteins were further selected for protein-protein interaction network analysis. A series of these analyses revealed that proteins associated with synapse and mitochondria might be linked to the therapeutic mechanism of NFP. Subsequent metabolic network analysis via genome-scale metabolic models that represent the three mouse groups showed that there were significant changes in metabolic fluxes of mitochondrial carnitine shuttle pathway and mitochondrial beta-oxidation of polyunsaturated fatty acids. Conclusion: Our results suggested that the therapeutic effects of NFP on AD were associated with synaptic- and mitochondrial-related pathways, and they provided targets for further rigorous studies on precise understanding of the molecular mechanism of NFP.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.30-30
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• 2023

This study aims to establish the multi-reservoir operation system model in the Upper Mun River Basin which includes 5 main dams namely, Mun Bon (MB), Lamchae (LC), Lam Takhong (LTK), Lam Phraphoeng (LPP), and Lower Lam Chiengkrai (LLCK) Dams. The knowledge and AI technology were applied aiming to develop innovative prototype for SMART dam-reservoir operation in future. Two different sorts of reservoir operation system model namely, Fuzzy Logic (FL) and Constraint Programming (CP) as well as the development of rainfall and reservoir inflow prediction models using Machine Learning (ML) technique were made to help specify the right amount of daily reservoir releases for the Royal Irrigation Department (RID). The model could also provide the essential information particularly for the Office of National Water Resource of Thailand (ONWR) to determine the short-term and long-term water resource management plan and strengthen water security against flood and drought in this region. The simulated results of base case scenario for reservoir operation in the Upper Mun from 2008 to 2021 indicated that in the same circumstances, FL and CP models could specify the new release schemes to increase the reservoir water storages at the beginning of dry season of approximately 125.25 and 142.20 MCM per year. This means that supplying the agricultural water to farmers in dry season could be well managed. In other words, water scarcity problem could substantially be moderated at some extent in case of incapability to control the expansion of cultivated area size properly. Moreover, using AI technology to determine the new reservoir release schemes plays important role in reducing the actual volume of water shortfall in the basin although the drought situation at LTK and LLCK Dams were still existed in some periods of time. Meanwhile, considering the predicted inflow and hydrologic factors downstream of 5 main dams by FL model and minimizing the flood volume by CP model could ensure that flood risk was considerably minimized as a result of new release schemes.

• 대한본초학회지
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• 제38권5호
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• pp.85-95
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• 2023

Objectives : This study was intended to reveal the chemical profiles of aerial(leaf, stem) and underground(rhizome, radix) parts of wild ginseng, and to investigate their anti-aging effects on human skin cells. Methods : Wild ginseng, estimated for over 20 years, was divided into the aerial and underground parts. Total phenolic contents of each extracts were measured using a Folin-ciocalteu method. The contents of 18 amino acids, 8 minerals and 27 ginsenosides were determined by GC-FID, ICP-MS and LC-MS, respectively. The anti-aging effects, including the radical scavenging activity, the activation of mitochondrial function on human fibroblasts, and the proliferation activity on human keratinocyte progenitor cells, for the whole plant and underground part of wild ginseng were evaluated. Results : The total phenolic acids, amino acids, and minerals in the aerial part were more than twice as high as in the underground part. Compared to the cultivated ginseng root, there were various types of ginsenosides in both parts of wild ginseng, and the total amount was more than twice as high. In particular, the aerial part significantly contained ginsenoside F1, F2, C-Mc1, and C-O, and the distinctive patterns that distinguish each parts of wild ginseng from the cultivated ginseng root were derived. The whole plant and underground part of wild ginseng exhibited significant antioxidant effect(14.3-45.6%), activation of mitochondrial membrane potential(105.5-120.1%), and cell proliferation(112.1-125.4%). Conclusions : The entire plant and underground part of wild ginseng are high value-added plants and have beneficial effects on skin anti-aging properties through its abundant metabolites.