• Journal of Microbiology and Biotechnology
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• v.21 no.2
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• pp.204-211
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• 2011

To yield high concentrations of protein expressed by genetically modified Escherichia coli, it is important that the bacterial strains are cultivated to high cell density in industrial bioprocesses. Since the expressed target protein is mostly accumulated inside the E. coli cells, the cellular product formation can be directly correlated to the bacterial biomass concentration. The typical way to determine this concentration is to sample offline. Such manual sampling, however, wastes time and is not efficient for acquiring direct feedback to control a fedbatch fermentation. An E. coli K12-derived strain was cultivated to high cell density in a pressurized stirred bioreactor on a pilot scale, by detecting biomass concentration online using a capacitance probe. This E. coli strain was grown in pure minimal medium using two carbon sources (glucose and glycerol). By applying exponential feeding profiles corresponding to a constant specific growth rate, the E. coli culture grew under carbon-limited conditions to minimize overflow metabolites. A high linearity was found between capacitance and biomass concentration, whereby up to 85 g/L dry cell weight was measured. To validate the viability of the culture, the oxygen transfer rate (OTR) was determined online, yielding maximum values of 0.69 mol/l/h and 0.98mol/l/h by using glucose and glycerol as carbon sources, respectively. Consequently, online monitoring of biomass using a capacitance probe provides direct and fast information about the viable E. coli biomass generated under aerobic fermentation conditions at elevated headspace pressures.

• Journal of The Korean Society of Agricultural Engineers
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• v.54 no.1
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• pp.47-54
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• 2012

Agriculture is dependable to weather condition and its change so that it is necessary to understand the impacts of climatic change. The aim of this study is to analyze the change of consumptive use of water and rice yield due to climate change using CERES-Rice. In this study, the weather data of three emission scenario of A1B, A2 and B1 created from CGCM (Coupled General Circulation Model) were used from 2011 to 2100, and downscaled daily weather data were simulated using LARS-WG (Long Ashton Research Station Weather Generator). The input data for cultivated condition for simulating CERSE (Crop-Environment Resource Synthesis)-Rice were created referring to standard cultivation method of paddy rice in Korea. The results showed that consumptive uses of water for paddy rice were projected decreasing to 4.8 % (2025s), 9.1 % (2055s), 12.6 % (2085s) comparing to the baseline value of 403.5 mm in A2 scenario. The rice yield of baseline was 450.7 kg/10a and projected increasing to -0.4 % (2025s), 3.9 % (2055s), 17.5 % (2085s) in A1B scenario. The results demonstrated relationships between consumptive use of water and rice yields due to climate change and can be used for the agricultural water resources development planning and cultivation method of paddy rice for the future.

• YAKHAK HOEJI
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• v.48 no.6
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• pp.352-357
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• 2004

Atrial myocytes have two functionally separate groups of ryanodine receptors (RyRs): those at the periphery colocalized with L-type $Ca^{2+}$channels (DHPRS) and those a t the cell interior not associated with DHPRs. $Ca^{2+}$ current ($I_{ca}$) directly gates peripheral RyRs on action potential and the subsequent peripheral $Ca^{2+}$ release propagates into the center of atrial myocytes. The mechanisms that regulate the $Ca^{2+}$+ propagation wave remain Poorly understood. Using 2-D confocal$Ca^{2+}$ imaging, we examined the role of inositol 1,4,5-trisphosphate receptor (IP $_3R$) and mitochondria on ($I_{ca}$)- gated local $Ca^{2+}$ signaling in rat atrial myocytes. Blockade of IP $_3R$ by xestospongin C (XeC) partially suppressed the magnitudes of I ca-gated central and peripheral $Ca^{2+}$ releases with no effect on $I_{ca}$. Mitochondrial staining revealed that mitochondria were aligned with ${\thickapprox}2-{\mu}m$ separations in the entire cytoplasm of ventricular and atrial myocytes. Membrane depolarization induced rapid mitochondrial $Ca^{2+}$ rise and decay in the cell periphery with slower rise in the center, suggesting that mitochondria may immediately uptake cytosolic $Ca^{2+}$, released from the peripheral SR on depolarization, and re-release the $Ca^{2+}$ into the cytosol to activate neighboring central RyRs. Our data suggest that the activation of IP $_3R$ and mitochondrial $Ca^{2+}$ handing on action potential may serve as a cofactor for the $Ca^{2+}$ propagation from the DHPR-coupled RyRs to the DHPR-uncoupled RyRs with large gaps between them.

• Safety and Health at Work
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• v.11 no.3
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• pp.291-300
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• 2020

Background: The study aimed to determine the association of individual cognitive ability in late midlife with labor market participation among older workers. Methods: This prospective cohort study estimates the risk of long-term sickness absence, disability pension, early retirement, and unemployment from scores on the Intelligenz-Struktur-Test 2000R by combining data from 5076 workers from the Copenhagen Aging and Midlife Biobank with a register on social transfer payments. Analyses were stepwise adjusted for age, gender, physical and psychosocial work environment, health behaviors, occupational social class, education, and chronic diseases. Results: In the fully adjusted model, low cognitive ability (≥1 standard deviation below the mean for each gender) and high cognitive ability (≥1 standard deviation above the mean for each gender) were not associated with risk of any of the four labor market outcomes. Conclusion: Individual cognitive ability in late midlife was not associated with risk of long-term sickness absence, disability pension, early retirement, and unemployment in the fully adjusted model. Thus, no direct effect of individual cognitive ability in late midlife was observed on the risk of permanently or temporarily leaving the labor market.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.9
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• pp.1449-1457
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• 2018

Objective: The objective was to evaluate feed intake, digestibility, body weight change and carcass characteristics of sheep fed a basal diet of hay supplemented with banana leaves and silver leaf desmodium. Methods: Thirty yearling lambs with an average initial body weight of $15.85{\pm}1.6kg$ were grouped into six blocks of five rams in each block. The treatments were: hay alone (T1), hay+100% banana leaf (T2), hay+67% banana leaf+33% desmodium leaf (T3), hay+33% banana leaf+67% desmodium leaf (T4), andhay+100% desmodium leaf (T5). Three hundred grams of treatment diets were offered daily on as fed basis. The feeding and digestibility trials lasted for 84 and 7 days, respectively, followed by carcass evaluation. Results: The total dry matter (DM) intake for T3, T4, and T5 were greater (p<0.05) than those fed T1 and T2 diets. The lowest (p<0.05) organic matter (OM) intake was recorded in rams reared on T1 diet. The total crude protein (CP) intake was in the following order: T5>T4>T3>T2>T1. Ram lambs receiving supplementary diets had higher (p<0.05) DM, OM, CP, neutral detergent fiber and acid detergent fiber digestibility compared with the control diet. The empty body weight and slaughter weight was highest (p<0.05) in rams receiving T3, T4, and T5 diets. The average daily gain and feed conversion efficiency was highest (p<0.05) in rams receiving the supplementary diets. The dressing percentage on the basis of hot carcass weight linearly increased with increasing levels of desmodium. Rams reared on supplementary diet had higher (p<0.05) rib eye area compared with the control diet. Conclusion: In conclusion, when banana leaf is used as a supplement to poor quality grass, better body weight gain was obtained when fed in combination with desmodium.

• Analytical Science and Technology
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• v.30 no.1
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• pp.1-9
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• 2017

Starch is a mixture of amylose (AMY) and amylopectin (AMP) which are different in physical properties such as molar mass (M), rms radius ($R_g$) and hydrodynamic diameter ($d_H$). The rheological and functional properties of starch are influenced by various factors including the molecular size, molar mass distribution (MD) and the concentration ratio of AMY and AMP. It is also important to analyze proteinaceous material in starch as they affect the flavor and texture of food to which starch is added. In this study, asymmetrical flow field-flow fractionation (AF4) was employed for separation and quantitation of AMY and AMP in starches (Amaranth, potato, taros and quinoa). AF4 was coupled with a multi-angle light scattering (MALS) and a refractive index (RI) detector for determination of the absolute M, MD and molecular structure. It was found that AMP has the M and $R_g$ ranging $3.7{\times}10^7{\sim}6.5{\times}10^8g/mol$ and 84 ~ 250 nm, respectively. Also the existence of branch was confirmed in higher M. In addition, proteinaceous material in starch was analyzed by AF4 coupled with a fluorescence detector (FS) after fluorescence-labeling. AF4-FS with fluorescence-labelling showed a potential for investigation on existence of proteinaceous material and the interaction between proteinaceous material and polysaccharide in starch.

• Progress in Medical Physics
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• v.13 no.4
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• pp.218-223
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• 2002

Stereotactic radiosurgery for intracranial lesion is well established since the Lars Leksell first introduced radiosurgery concept in 1951 Its use in the treatment of spinal lesion has been limited by the availability of effective immobilization devices. The first clinical experience of the spinal stereotactic radiosurgery technique was reported by Hamilton AJ. in 1995. Recently, Optic-guided patient positioning technique for extracranial stereotactic radiosurgery was developed and reported. This study is for assess the target positioning accuracy of the optic guided patient positioning system Exactrac (BrainLab., Inc, Germany). We have designed phantom for assess the accuracy of spinal stereotactic radiosurgery The infrared reflective body markers attached to the relatively immobile part of the body and a series of 2 mm CT images was taken. The image sets were transferred to the planning computer. During the radiosurgery treatment, we measure the real-time display showing the positioning values from Exactrac computer. And we compare the isocenter deviation from irradiated center point of the film which was mounted on the lesion site of the phantom and pin hole site of that film. The accuracy of the ExacTrac system in positioning a target point shows enough for the clinical applications.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.1
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• pp.14-21
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• 2015

Objective: It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast majority of 3PN IVF zygotes are of dispermic origin and thus more likely to have two centrioles, the 3PN ICSI zygotes are digynic in origin and therefore, more likely to have one centriole. In the present study, we examine whether the parental origin of 3PN embryos correlates with the karyotype. Methods: The karyotype of each nucleus was estimated using four sequential fluorescence in situ hybridizations-each with two probes-resulting in quantitative information of 8 different chromosomes. The karyotypes were then compared and correlated to the parental origin. Results: 3PN ICSI embryos displayed a significantly larger and more coordinated reduction from the assumed initial 3 sets of chromosomes than 3PN IVF embryos. Conclusion: The differences in the parental origin-and hence the number of centrioles-between the 3PN IVF and the 3PN ICSI zygotes are likely to be the cause of the differences in karyotypes.

• Nuclear Engineering and Technology
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• v.44 no.5
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• pp.507-522
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• 2012

When decommissioning a nuclear power plant, it is difficult to make an appropriate plan to ensure sufficient space for temporary placement and conveyance operations of dismantling targets. This paper describes a system to support temporary placement and conveyance operations using augmented reality (AR). The system employs a laser range scanner to measure the three-dimensional (3D) information of the environment and a dismantling target to produce 3D surface polygon models. Then, the operator simulates temporary placement and conveyance operations using the system by manipulating the obtained 3D model of the dismantling target in the work field. Referring to the obtained 3D model of the environment, a possible collision between the dismantling target and the environment is detectable. Using AR, the collision position is presented intuitively. After field workers evaluated this system, the authors concluded that the system is feasible and acceptable to verify whether spaces for passage and temporary storage are sufficient for temporary placement and conveyance operations. For practical use in the future, some new functions must be added to improve the system. For example, it must be possible for multiple workers to use the system simultaneously by sharing the view of dismantling work.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.446-446
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• 2012

농업은 다른 산업과 달리 원천적으로 기후 조건과 변화에 크게 좌우되는 분야로, 기후변화로 인한 영향에 가장 민감한 분야라고 할 수 있다. 안정적이고 지속적인 작물 생산을 위해서는 기후변화가 농업수자원에 미치는 영향에 대하여 정확히 파악하고, 이로 인해 발생할 수 있는 부정적 효과를 최소화하기 위한 연구가 필요하다. 특히 기온 상승, 강수량 및 강우강도 변화, 증발산량 및 일조시간 변화 등의 기후변화는 우리나라의 가뭄 발생의 양상에도 변화를 야기하게 될 것이다. 따라서 현 상황을 바탕으로 미래에 발생할 가뭄에 대하여 예측하고, 그 취약성을 줄이기 위한 합리적인 계획이 필요하다. 즉 기후변화에 대처하기 위해서는 향후 발생할 수 있는 가뭄의 특성을 파악하여 미래 수자원 관리에 활용하기 위한 가뭄특성 분석이 필요하다. 가뭄은 기상학적 가뭄, 기후학적 가뭄, 농업적 가뭄, 대기학적 가뭄, 수문학적 가뭄, 사회경제적 가뭄 등으로 구분할 수 있는데, 일반적으로 강우량 등의 기상조건을 분석하는 방법에서부터 저수량과 유역 유출량, 그리고 토양수분 등의 수문학적 조건들로 가뭄을 분석하는 방법들까지 매우 다양하다. 가뭄의 정량화는 가뭄을 표현하는 대상의 특성에 따라 평가방법이 달라질 수 있다. 가뭄의 경향이나 그 정도를 파악하기 위해서는 하나의 가뭄 지수가 아닌 다양한 항목을 바탕으로 평가가 이루어져 한다. 현재 기후변화와 관련한 가뭄 연구에 있어서 기상학적 가뭄지수인 SPI (Standardized Precipitation Index) 중심으로 많은 연구 이루어졌을 뿐, 농업적 가뭄지수를 바탕으로 한 연구는 이루어지지 않고 있다. 따라서 본 연구에서는 기후변화에 따른 우리나라의 농업가뭄 특성을 분석하기 위하여 토양수분지수 (Soil Moisture Index)를 이용하여 중권역별 가뭄 평가하고 그 변화를 분석하였다. 본 연구를 위하여 이를 위하여 CGCM3.1 (Coupled Global Climate Model Ver. 3.1) 및 LARS-WG (Long Ashton Research Station Weather Generator)를 이용하여 2011년부터 2100년까지의 A1B, A2 및 B1 시나리오별로 기상자료를 생성하고, 이를 바탕으로 SMI 지수를 산정하여 유역별 가뭄 발생 빈도 및 심도를 시나리오별로 분석하였다. 본 연구 결과는 향후 기후변화로 인한 농업가뭄 발생의 양상 및 특성을 파악하고 전망함으로써, 추후 발생할 수 있는 부정적 효과를 최소화하기 위한 대응 전략 및 농업수자원 정책의 기초 자료로 활용될 수 있을 것으로 판단된다.