• Clinical and Experimental Pediatrics
• /
• 제63권7호
• /
• pp.272-277
• /
• 2020

Background: The optimal management of perianal abscess in children is controversial. Purpose: To evaluate the efficiency of conservative treatment of perianal abscess in children and identify parameters that predict therapy failure. Methods: All cases of children younger than 14 years of age with perianal abscesses between 2001-2016 were evaluated. Results: Of the 113 enrolled patients, 64 underwent subsequent surgery for advanced disease (primary surgery group). Conservative treatment was initiated in 49 patients (primary conservative group) but was stopped because of inefficiency in 25 patients, who were referred for surgery after a median 7.03 days (range, 2 to 16 days). The other 24 patients (48%) initially achieved complete remission after conservative treatment, but 10 were readmitted after a median 34 months (range, 3 to 145 months) with recurrent disease. There were no significant differences in permanent success after conservative treatment between infants (10 of 29, 34%) and older children (4 of 20 [20%], P=0.122). Overall, conservative treatment alone was effective in only 14 of 113 patients. Recurrence after surgery occurred in 16 patients (25%) in the primary surgery group and 11 patients (22%) in the primary conservative group (P=0.75). Univariate analysis of predictors for conservative treatment failure revealed inflammatory values (C-reactive protein and white blood count, P=0.017) and abscess size (P=0.001) as significant parameters, whereas multivariate analysis demonstrated that only abscess size (odds ratio, 3.37; P=0.023) was significant. Conclusion: Conservative treatment of perianal abscess is permanently efficient in only a minority of children but is not associated with a higher recurrence rate after subsequent surgery. Abscess size is a predictor for therapy failure.

• Journal of Microbiology and Biotechnology
• /
• 제21권2호
• /
• pp.204-211
• /
• 2011

To yield high concentrations of protein expressed by genetically modified Escherichia coli, it is important that the bacterial strains are cultivated to high cell density in industrial bioprocesses. Since the expressed target protein is mostly accumulated inside the E. coli cells, the cellular product formation can be directly correlated to the bacterial biomass concentration. The typical way to determine this concentration is to sample offline. Such manual sampling, however, wastes time and is not efficient for acquiring direct feedback to control a fedbatch fermentation. An E. coli K12-derived strain was cultivated to high cell density in a pressurized stirred bioreactor on a pilot scale, by detecting biomass concentration online using a capacitance probe. This E. coli strain was grown in pure minimal medium using two carbon sources (glucose and glycerol). By applying exponential feeding profiles corresponding to a constant specific growth rate, the E. coli culture grew under carbon-limited conditions to minimize overflow metabolites. A high linearity was found between capacitance and biomass concentration, whereby up to 85 g/L dry cell weight was measured. To validate the viability of the culture, the oxygen transfer rate (OTR) was determined online, yielding maximum values of 0.69 mol/l/h and 0.98mol/l/h by using glucose and glycerol as carbon sources, respectively. Consequently, online monitoring of biomass using a capacitance probe provides direct and fast information about the viable E. coli biomass generated under aerobic fermentation conditions at elevated headspace pressures.

• 한국농공학회논문집
• /
• 제54권1호
• /
• pp.47-54
• /
• 2012

Agriculture is dependable to weather condition and its change so that it is necessary to understand the impacts of climatic change. The aim of this study is to analyze the change of consumptive use of water and rice yield due to climate change using CERES-Rice. In this study, the weather data of three emission scenario of A1B, A2 and B1 created from CGCM (Coupled General Circulation Model) were used from 2011 to 2100, and downscaled daily weather data were simulated using LARS-WG (Long Ashton Research Station Weather Generator). The input data for cultivated condition for simulating CERSE (Crop-Environment Resource Synthesis)-Rice were created referring to standard cultivation method of paddy rice in Korea. The results showed that consumptive uses of water for paddy rice were projected decreasing to 4.8 % (2025s), 9.1 % (2055s), 12.6 % (2085s) comparing to the baseline value of 403.5 mm in A2 scenario. The rice yield of baseline was 450.7 kg/10a and projected increasing to -0.4 % (2025s), 3.9 % (2055s), 17.5 % (2085s) in A1B scenario. The results demonstrated relationships between consumptive use of water and rice yields due to climate change and can be used for the agricultural water resources development planning and cultivation method of paddy rice for the future.

• 약학회지
• /
• 제48권6호
• /
• pp.352-357
• /
• 2004

Atrial myocytes have two functionally separate groups of ryanodine receptors (RyRs): those at the periphery colocalized with L-type $Ca^{2+}$channels (DHPRS) and those a t the cell interior not associated with DHPRs. $Ca^{2+}$ current ($I_{ca}$) directly gates peripheral RyRs on action potential and the subsequent peripheral $Ca^{2+}$ release propagates into the center of atrial myocytes. The mechanisms that regulate the $Ca^{2+}$+ propagation wave remain Poorly understood. Using 2-D confocal$Ca^{2+}$ imaging, we examined the role of inositol 1,4,5-trisphosphate receptor (IP $_3R$) and mitochondria on ($I_{ca}$)- gated local $Ca^{2+}$ signaling in rat atrial myocytes. Blockade of IP $_3R$ by xestospongin C (XeC) partially suppressed the magnitudes of I ca-gated central and peripheral $Ca^{2+}$ releases with no effect on $I_{ca}$. Mitochondrial staining revealed that mitochondria were aligned with ${\thickapprox}2-{\mu}m$ separations in the entire cytoplasm of ventricular and atrial myocytes. Membrane depolarization induced rapid mitochondrial $Ca^{2+}$ rise and decay in the cell periphery with slower rise in the center, suggesting that mitochondria may immediately uptake cytosolic $Ca^{2+}$, released from the peripheral SR on depolarization, and re-release the $Ca^{2+}$ into the cytosol to activate neighboring central RyRs. Our data suggest that the activation of IP $_3R$ and mitochondrial $Ca^{2+}$ handing on action potential may serve as a cofactor for the $Ca^{2+}$ propagation from the DHPR-coupled RyRs to the DHPR-uncoupled RyRs with large gaps between them.

• Safety and Health at Work
• /
• 제11권3호
• /
• pp.291-300
• /
• 2020

Background: The study aimed to determine the association of individual cognitive ability in late midlife with labor market participation among older workers. Methods: This prospective cohort study estimates the risk of long-term sickness absence, disability pension, early retirement, and unemployment from scores on the Intelligenz-Struktur-Test 2000R by combining data from 5076 workers from the Copenhagen Aging and Midlife Biobank with a register on social transfer payments. Analyses were stepwise adjusted for age, gender, physical and psychosocial work environment, health behaviors, occupational social class, education, and chronic diseases. Results: In the fully adjusted model, low cognitive ability (≥1 standard deviation below the mean for each gender) and high cognitive ability (≥1 standard deviation above the mean for each gender) were not associated with risk of any of the four labor market outcomes. Conclusion: Individual cognitive ability in late midlife was not associated with risk of long-term sickness absence, disability pension, early retirement, and unemployment in the fully adjusted model. Thus, no direct effect of individual cognitive ability in late midlife was observed on the risk of permanently or temporarily leaving the labor market.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권9호
• /
• pp.1449-1457
• /
• 2018

Objective: The objective was to evaluate feed intake, digestibility, body weight change and carcass characteristics of sheep fed a basal diet of hay supplemented with banana leaves and silver leaf desmodium. Methods: Thirty yearling lambs with an average initial body weight of $15.85{\pm}1.6kg$ were grouped into six blocks of five rams in each block. The treatments were: hay alone (T1), hay+100% banana leaf (T2), hay+67% banana leaf+33% desmodium leaf (T3), hay+33% banana leaf+67% desmodium leaf (T4), andhay+100% desmodium leaf (T5). Three hundred grams of treatment diets were offered daily on as fed basis. The feeding and digestibility trials lasted for 84 and 7 days, respectively, followed by carcass evaluation. Results: The total dry matter (DM) intake for T3, T4, and T5 were greater (p<0.05) than those fed T1 and T2 diets. The lowest (p<0.05) organic matter (OM) intake was recorded in rams reared on T1 diet. The total crude protein (CP) intake was in the following order: T5>T4>T3>T2>T1. Ram lambs receiving supplementary diets had higher (p<0.05) DM, OM, CP, neutral detergent fiber and acid detergent fiber digestibility compared with the control diet. The empty body weight and slaughter weight was highest (p<0.05) in rams receiving T3, T4, and T5 diets. The average daily gain and feed conversion efficiency was highest (p<0.05) in rams receiving the supplementary diets. The dressing percentage on the basis of hot carcass weight linearly increased with increasing levels of desmodium. Rams reared on supplementary diet had higher (p<0.05) rib eye area compared with the control diet. Conclusion: In conclusion, when banana leaf is used as a supplement to poor quality grass, better body weight gain was obtained when fed in combination with desmodium.

• 분석과학
• /
• 제30권1호
• /
• pp.1-9
• /
• 2017

Starch is a mixture of amylose (AMY) and amylopectin (AMP) which are different in physical properties such as molar mass (M), rms radius ($R_g$) and hydrodynamic diameter ($d_H$). The rheological and functional properties of starch are influenced by various factors including the molecular size, molar mass distribution (MD) and the concentration ratio of AMY and AMP. It is also important to analyze proteinaceous material in starch as they affect the flavor and texture of food to which starch is added. In this study, asymmetrical flow field-flow fractionation (AF4) was employed for separation and quantitation of AMY and AMP in starches (Amaranth, potato, taros and quinoa). AF4 was coupled with a multi-angle light scattering (MALS) and a refractive index (RI) detector for determination of the absolute M, MD and molecular structure. It was found that AMP has the M and $R_g$ ranging $3.7{\times}10^7{\sim}6.5{\times}10^8g/mol$ and 84 ~ 250 nm, respectively. Also the existence of branch was confirmed in higher M. In addition, proteinaceous material in starch was analyzed by AF4 coupled with a fluorescence detector (FS) after fluorescence-labeling. AF4-FS with fluorescence-labelling showed a potential for investigation on existence of proteinaceous material and the interaction between proteinaceous material and polysaccharide in starch.

• 한국의학물리학회지:의학물리
• /
• 제13권4호
• /
• pp.218-223
• /
• 2002

두개외 병소 즉, 척추 등에 발생한 종양 혹은 혈관기형 등의 병소에 대해 침습적 수술이 불가한 경우 정위적 방사선 수술이 임상에서 시도되고 있다. 본 연구는 실험적으로 팬텀에 대해 척추 정위 방사선수술을 시행하고 결과 분석을 통하여 두개외 수술 목표점에 대한 방사선수술 위치 정확도를 평가하고자 한다. 이 실험을 위하여 팬텀을 특별히 고안하였으며 수술실에서 방사선 수술 목표점의 위치는 광학적 추적 장치를 이용하여 결정하였고 3 mm 미세다엽시준기(mMLC; mcro Multi-Leaf Collimator)를 사용하여 시술하였다. 팬텀의 목표점에 대한 방사선수술 오차는 $\pm$1 mm 이내였으며 분할방사선 수술 경우도 $\pm$1 mm 이내였다. 결과적으로 광학적 위치추적 장치를 이용한 두개외 목표점의 방사선 수술은 매우 정확하고 유용한 방법으로 판단된다.

• Clinical and Experimental Reproductive Medicine
• /
• 제42권1호
• /
• pp.14-21
• /
• 2015

Objective: It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast majority of 3PN IVF zygotes are of dispermic origin and thus more likely to have two centrioles, the 3PN ICSI zygotes are digynic in origin and therefore, more likely to have one centriole. In the present study, we examine whether the parental origin of 3PN embryos correlates with the karyotype. Methods: The karyotype of each nucleus was estimated using four sequential fluorescence in situ hybridizations-each with two probes-resulting in quantitative information of 8 different chromosomes. The karyotypes were then compared and correlated to the parental origin. Results: 3PN ICSI embryos displayed a significantly larger and more coordinated reduction from the assumed initial 3 sets of chromosomes than 3PN IVF embryos. Conclusion: The differences in the parental origin-and hence the number of centrioles-between the 3PN IVF and the 3PN ICSI zygotes are likely to be the cause of the differences in karyotypes.

• Nuclear Engineering and Technology
• /
• 제44권5호
• /
• pp.507-522
• /
• 2012

When decommissioning a nuclear power plant, it is difficult to make an appropriate plan to ensure sufficient space for temporary placement and conveyance operations of dismantling targets. This paper describes a system to support temporary placement and conveyance operations using augmented reality (AR). The system employs a laser range scanner to measure the three-dimensional (3D) information of the environment and a dismantling target to produce 3D surface polygon models. Then, the operator simulates temporary placement and conveyance operations using the system by manipulating the obtained 3D model of the dismantling target in the work field. Referring to the obtained 3D model of the environment, a possible collision between the dismantling target and the environment is detectable. Using AR, the collision position is presented intuitively. After field workers evaluated this system, the authors concluded that the system is feasible and acceptable to verify whether spaces for passage and temporary storage are sufficient for temporary placement and conveyance operations. For practical use in the future, some new functions must be added to improve the system. For example, it must be possible for multiple workers to use the system simultaneously by sharing the view of dismantling work.