• 한국방사선학회논문지
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• 제16권7호
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• pp.943-952
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• 2022

지방간 진단을 위한 검사로, 최근 초음파 검사와 혈액검사를 동시에 병행하여 시행하고 있다. 특히 혈액검사 중 hs-CRP의 경우, 심혈관 질환 뿐 아닌, 인체의 다양한 부위에 대한 염증 수치를 나타내는 지표로 사용되고 있다. 따라서 본 연구를 통해 비알콜성 지방간 정도에 따른 대사증후군 구성 요소와 간 기능 및 hs-CRP수치 등의 연관성을 분석해 지방간 진단 임상 지표로 활용하고자 연구를 진행하였다. 2021년 3월~2021년 8월 한국 건강관리 협회 광주 전남지부에서 복부 초음파 검사를 실시하여 비알콜 지방간이 관찰된 환자 중 대사증후군 구성요소와 간 기능 및 hs-CRP 검사를 모두 실시한 만 20세 이상, 남녀 총 1,139명의 피검사 수치 데이터를 대상으로 하였다. 남녀 전체를 대상으로 분석한 경우 경도 지방간 환자의 간 검사 수치가 AST 30 U/L, ALT 32.1 U/L hs-CRP 0.14 mg/dL로 중등도 지방간 환자의 혈액 검사 수치 AST 38 U/L, ALT 47.6 U/L, 54.9 IU/L, hs-CRP 0.22 mg/dL보다 낮았으며 통계적으로 유의했다(P<0.001). hs-CRP 검사의 경우 경도 지방간 환자보다 중등도 지방간 환자에서 통계적으로 유의할 만큼 높은 수치 차이를 나타낸 것으로 보아 hs-CRP 검사가 지방간 예방과 관리를 위한 임상적 데이터로도 활용될 수 있을 것으로 사료된다.

• 한국환경농학회지
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• 제42권4호
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• pp.286-296
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• 2023

This study was conducted to evaluate the effects of soil microbial fertilizer (SMF) containing Saccharomyces cerevisiae HS-1 and Streptococcus thermophiles HS-2 on the growth of creeping bentgrass. For the pot experiment, the treatments were as follows: no fertilizer (NF), control (3 N g/m²/month), SMF-1 (control+SMF 2 mL/m²/time), and SMF-2 (control+SMF 4 mL/m²/time). For the plot experiment, the treatments were as follows: NF, control, SMFp-1 (control+SMF 1 mL/m²/time), SMFp-2 (control+SMF 2 mL/m²/time), and SMFp-3 (control+SMF 4 mL/m²/time). In the pot experiment, visual turfgrass quality and the uptake amount of nitrogen (N) and potassium (K) were increased under the SMF treatments, whereas the content of chlorophyll (a, b, and a+b) and clipping yield were not considerably different compared with the control. In the pot experiment, the amount of SMF positively correlated with visual turfgrass quality and uptake amount of N and K. In the plot experiment, turfgrass density was increased by 12.9-19.2% under SMFp treatments compared with the control. These results indicated that the application of SMF containing Sa. cerevisiae HS-1 and St. thermophiles HS-2 improved the quality, density, and growth of creeping bentgrass via prompting the uptake of N and K.

• Molecules and Cells
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• 제42권1호
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• pp.87-95
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• 2019

Long interspersed element-1 (LINE-1 or L1) is an autonomous retrotransposon, which is capable of inserting into a new region of genome. Previous studies have reported that these elements lead to genomic variations and altered functions by affecting gene expression and genetic networks. Mounting evidence strongly indicates that genetic diseases or various cancers can occur as a result of retrotransposition events that involve L1s. Therefore, the development of methodologies to study the structural variations and interpersonal insertion polymorphisms by L1 element-associated changes in an individual genome is invaluable. In this study, we applied a systematic approach to identify human-specific L1s (i.e., L1Hs) through the bioinformatics analysis of high-throughput next-generation sequencing data. We identified 525 candidates that could be inferred to carry non-reference L1Hs in a Korean individual genome (KPGP9). Among them, we randomly selected 40 candidates and validated that approximately 92.5% of non-reference L1Hs were inserted into a KPGP9 genome. In addition, unlike conventional methods, our relatively simple and expedited approach was highly reproducible in confirming the L1 insertions. Taken together, our findings strongly support that the identification of non-reference L1Hs by our novel target enrichment method demonstrates its future application to genomic variation studies on the risk of cancer and genetic disorders.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.3963-3970
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• 2012

Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at $60^{\circ}C$, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng $mL^{-1}$ and 0.05-0.5 ng $mL^{-1}$. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng $mL^{-1}$; detected levels of benzene were 0.05-2.20 ng $mL^{-1}$.

• Mass Spectrometry Letters
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• 제4권1호
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• pp.18-20
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• 2013

Headspace solid phase micro-extraction gas chromatography/flame ionization detection (HS-SPME-GC/FID) method was compared with headspace gas chromatography/mass selective detection (HS-GC/MS). Organic solvent-spiked urine as well as urine samples from workspace was analyzed under optimal condition of each method. Detection limit of each compound by HS-SPME-GC/FID was $3.4-9.5{\mu}g/L$, which enabled trace analysis of organic solvents in urine. Linear range of each organic solvent was $10-400{\mu}g/L$, with fair correlation coefficient between 0.992 and 0.999. The detection sensitivity was 4 times better than HS-GC/MS in selected ion monitoring (SIM) mode. Accuracy and precision was confirmed using commercial reference material, with accuracy around 90% and precision less than 4.6% of coefficient of variance. Among 48 urine samples from workplace, toluene was detected from 45 samples in the range of $20-324{\mu}g/L$, but no other solvents were found. As a method for trace analysis, SPME HS GC/FID showed high sensitivity for biological monitoring of organic solvent in urine.

• 한국토양비료학회지
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• 제43권5호
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• pp.659-666
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• 2010

선행연구에서 근권 토양으로부터 분리된 Lysobacter antibioticus HS124 (HS124)는 lytic enzyme으로써 chitinase, gelatinase, lipase 및 protease 등의 효소와 항생물질인 4-hydroxyphenylacetic acid (4-HPAA)를 생성하였다. 본 실험에서는 HS124를 이용하여 배추좀나방 (diamondback moth, Plutella xylostella L.) 3~4령 유충의 살충활성을 검정하였다. HS124 배양액을 배추좀나방 유충에 처리하였을 때 유충은 파괴되어 분해되었다. HS124가 생성하는 4-HPAA를 유충에 처리하였을 때 처리 농도가 높을수록 살충율은 증가하였으며, HS124 배양액에 Tween 80을 첨가하였을 때 첨가하지 않은 처리구보다 살충율이 1.4배 높았다. 한편 화학 살충제 (IS), HS124 배양액 (HS124), 식물추출물 (매직파이; MP), HS124 배양액+식물추출물 (HS124+MP) 및 멸균수 (SDW)를 이용하여 배추좀나방 유충의 살충율을 검정하였다. HS124+MP 처리구에서 가장 높은 살충율을 나타내었고, IS, MP, HS124 및 SDW 처리구 순으로 살충율이 감소하였다. HS124 처리구는 대조구인 멸균수처리구보다 31% 높은 살충율을 나타내었고, HS124+MP처리구 보다 40% 낮은 살충율을 나타내었다. 이러한 결과로 보아 항생물질과 다양한 lytic enzyme을 생성하는 L. antibioticus HS124 배양액과 식물추출물의 혼합제제는 배추좀나방의 생물학적 방제제로써 가치가있다고 사료된다.

• 한국토양비료학회지
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• 제49권4호
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• pp.393-400
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• 2016

Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed destructive alterations in the hyphal morphology of F. graminearum and almost degraded with $1,000{\mu}g\;mL^{-1}$ concentration. For further study, the antifungal compound extracted from the n-butanol crude extract of L. antibioticus HS124 was identified as N-Butyl-tetrahydro-5-oxofuran-2-carboxamide ($C_9H_{16}NO_3$) using NMR ($^1H-NMR$, $^{13}C-NMR$, $^1H-^1H\;COSY$, HMBC, and HMQC), and HR-ESI-MS analysis. To our knowledge, N-Butyl-tetrahydro-5-oxofuran-2-carboxamide may be a novel compound with molecular weight of 186.1130. The minimum inhibitory concentration value of antifungal compound was $62.5{\mu}g\;mL^{-1}$ against F. graminearum. In an in vivo pot experiment, crown rot disease from F. graminearum was inhibited when wheat seeds were treated with both HS124 culture and F. graminearum. Growth of wheat seedling was enhanced by treatment of HS124 compared to control. Our results suggest that L. antibioticus HS124 characterized in this study could be successfully used to control F. graminearum and could be used as an alternative to chemical fungicides in modern agriculture.

• Journal of Periodontal and Implant Science
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• 제45권1호
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• pp.14-22
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• 2015

Purpose: The purpose of this study was to compare serum amyloid A (SAA) protein levels with high-sensitive C-reactive protein (hs-CRP) levels as markers of systemic inflammation in patients with chronic periodontitis. The association of serum titers of antibodies to periodontal microbiota and SAA/hs-CRP levels in periodontitis patients was also studied. Methods: A total of 110 individuals were included in this study. Patients were assessed for levels of hs-CRP and SAA. Nonfasting blood samples were collected from participants at the time of clinical examination. The diagnosis of adipose tissue disorders was made according to previously defined criteria. To determine SAA levels, a sandwich enzyme-linked immunosorbent assay was utilized. Paper points were transferred to a sterile tube to obtain a pool of samples for polymerase chain reaction processing and the identification of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Tannerella forsythia. The serum level of IgG1 and IgG2 antibodies to P. gingivalis, A. actinomycetemcomitans, and T. forsythia was also determined. Results: SAA and hs-CRP levels were higher in periodontitis patients than in controls (P<0.05). In bivariate analysis, high levels of hs-CRP (>3 mg/L) and SAA (>10 mg/L) were significantly associated with chronic periodontitis (P=0.004). The Spearman correlation analysis between acute-phase proteins showed that SAA positively correlated with hs-CRP (r=0.218, P=0.02). In the adjusted model, chronic periodontitis was associated with high levels of SAA (odds ratio [OR], 5.5; 95% confidence interval [CI], 1.6-18.2; P=0.005) and elevated hs-CRP levels (OR, 6.1, 95% CI, 1.6-23.6; P=0.008). Increased levels of serum IgG2 antibodies to P. gingivalis were associated with high levels of SAA (OR, 3.6; 95% CI, 1.4-8.5; P=0.005) and high concentrations of hs-CRP (OR, 4.3; 95% CI, 1.9-9.8; P<0.001). Conclusions: SAA and hs-CRP concentrations in patients with chronic periodontitis are comparably elevated. High serum titers of antibodies to P. gingivalis and the presence of periodontal disease are independently related to high SAA and hs-CRP levels.

• 광물과 암석
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• 제36권1호
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• pp.19-32
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• 2023

본 연구에서는 S광산의 광산폐기물 적치장 침출수에 함유된 불소의 처리를 위해(1) Ca계 물질을 이용한 공침법; (2) 활성탄과 비산회를 이용한 흡착법; (3) 알럼을 이용한 응집침전법 등을 이용한 불소(초기 농도 9.5 mg/L) 제거 실험을 수행하였다. Ca계 물질을 이용한 공침법의 경우 최대 65.6%, 활성탄 흡착법 27.9%, 비산회 흡착법 71.5%, 알럼을 이용한 응집침전법은 최대 96.6%의 불소 제거 효율을 보였다. 또한 불소의 제거에 있어 높은 제거 효율을 보인 알럼을 이용하여 모의 침출수를 대상으로 실험실 내 반응조에서 수행한 연속 처리 공정 가능성 검토결과, 저농도(6.4 mg/L), 고농도(15.7 mg/L) 모의 침출수를 불소의 국내 청정 지역 배출수 허용 기준인 3 mg/L이하로 처리하는 것이 가능한 것으로 나타났다. 또한 벤치 규모 반응조 운영을 통한 현장 불소 제거 실험 결과, 적정한 운영 및 관리를 하는 경우 불소의 방류수 수질기준을 만족시킬 수 있음을 확인하였다.

• 한국산업보건학회지
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• 제31권2호
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• pp.119-127
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• 2021

Objectives: We aimed to find the relationships between employment status and hs-CRP level among Korean wage workers using the 2016 Korean National Health and Nutrition Examination (KNHANE, revised). Methods: This study was conducted on 1,937 daytime wage workers over the age of 19 and within a normal weight range (18.5≤BMI≺25). Regular workers were defined as those granted an employment guarantee until reaching retirement age, and non-regular workers were defined as temporary, non-typical, dispatched, short-term workers and contractors. For hs-CRP, three divisions were classified as recommended by the Centers for Disease Control and Prevention (CDC) and American Heart Association (AHA) with less than 1.0 mg/L indicating low risk of cardiovascular disease, above 1.0 mg/L and below 3.0 mg/L considered moderate risk, and more than 3.0 mg/L indicating high risk. To find the relationship between work type and hs-CRP level in Korean wage workers, multinominal logistic regression analysis was performed. Results: For non-regular workers, the odds ratio of the cardiovascular moderate-risk group and cardiovascular high-risk group was statistically significant compared to regular workers. After adjusting for factors such as gender, age, subjective health status, income, education, smoking, and physical labor, the odds ratio of the cardiovascular high-risk group was statistically significant. Conclusions: In this study, the relationship between non-regular workers and high hs-CRP level was examined. Based on this, institutional strategies should be pursued to prevent and manage cardiovascular disease among non-regular workers.