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Environmental Studies in the Lower Part of the Han River VIII. Physicochemical Factors Contributing to Variation of Phytoplankton Communities (한강 하류의 환경학적 연구 VIII. 식물플랑크톤 군집의 변화에 미치는 물리 화학적 요인)

  • Kwon, Oh-Youn;Jung, Seung-Won;Lee, Jin-Hwan
    • Korean Journal of Ecology and Environment
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    • v.39 no.3 s.117
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    • pp.340-351
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    • 2006
  • To reveal physicochemical factors contributing to variation of phytoplankton communities, the study was carried out biweekly at 6 stations from Feb. 2004 to Feb. 2005 in the lower part of the Han River, Korea. As results, water temperature was changed from $0.3^{\circ}C$ to $26.6^{\circ}C$, pH: 6.6${\sim}$9.1, DO: 1.89${\sim}$22.23 mg $L^{-1}$, BOD: 0.38${\sim}$9.20 mg $L^{-1}$, COD: 1.4${\sim}$15.2 mg $L^{-1}$, Conductivity: $62.5{\sim}500.0\;{\mu}s\;cm^{-1}$, SS: 3.00${\sim}$159.3 mg $L^{-1}$, and Chl a $1.7{\sim}71.3\;{\mu}g\;L^{-1}$. Phytoplankton standing crops ranged from min. $3.6{\times}10^2\;cells\;mL^{-1}$ (July 2004, St. 3) to max. $2.3{\times}10^4\;cells\;mL^{-1}$ (Feb. 2005, St. 6), and mean of those varied from $5.9{\times}10^3\;cells\;mL^{-1}$in spring, $2.1{\times}10^3\;cells\;mL^{-1}$ in summer, $4.1{\times}10^3\;cells\;mL^{-1}$ in autumn and $8.5{\times}10^3\;cells\;mL^{-1}$ in winter, respectively. In order to investigate factors influencing the total phytoplankton standing crops a multiple regression analysis was adopted for the correlation between standing crops and environmental factors. The coefficient of determination ($R^2$) value of the regression was 0.465, it showed that environmental factors which predominantly influenced phytoplankton standing crops were water temperature, COD, $NO_2-N$, $PO_4-N$, Discharge and pH. six stations could be divided into 3 groups based on similarity index in terms of environmental factors. In ANOVA analysis for physicochemical and biological factors, water temperature, chlorophyll a, silicate, phytoplankton standing crops were the same group differed little from stations. However, Station 1and 2 were grouped followed in dissolved oxygen, conductivity, COD, nitrite, nitrate, ammonia and phosphate, and Station 3, 4 and 5 were followed in dissolved oxygen, conductivity, pH and phosphate.

Metabolite Chemical Composition of the Bletilla striata (Thunb.) Reichb. f. Endophyte Penicillium oxalicum

  • Ran Liu;Xuehua Han;Jing Gao;Min Luo;Dale Guo;Guangzhi Wang
    • Mycobiology
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    • v.51 no.3
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    • pp.148-156
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    • 2023
  • Penicillium oxalicum strain can be isolated from the Bletilla striata (Thunb.) Reichb. f. tubers. Its solid-state fermentation products are concentrated by percolation extraction. Separation and purification have been conducted to the ethyl acetate extracts by preparative HPLC. Based on the use of spectrometry, we have determined 17 known compounds, 12,13-dihydroxy-fumitremorgin C (1), pseurotin A (2), tyrosol (3), cyclo-(L-Pro-L-Val) (4), cis-4-hydroxy-8-O-methylmellein (5), uracil (6), cyclo-(L-Pro-L-Ala) (7), 1,2,3,4-tetrahydro-4-hydroxy-4-quinolin carboxylic acid (8), cyclo-(Gly-L-Pro) (9), 2'-deoxyuridine (10), 1-(b-D-ribofuranosyl)thymine (11), cyclo-(L-Val-Gly) (12), 2'-deoxythymidine (13), cyclo-(Gly-D-Phe) (14), cyclo-L-(4-hydroxyprolinyl)-D-leucine (15), cyclo-(L)-4-hydroxy-Pro-(L)-Phe (16), uridine (17). Here, we report compounds 1-3, 5, 7-8, 11-12, 14-17 are first found and isolated from this endophyte.

The BioFlex System as a Dynamic Stabilization Device : Does It Preserve Lumbar Motion?

  • Zhang, Ho-Yeol;Park, Jeong-Yoon;Cho, Bo-Young
    • Journal of Korean Neurosurgical Society
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    • v.46 no.5
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    • pp.431-436
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    • 2009
  • Objective : This study examines whether functional motion is present at one or more years after Bioflex System placement. BioFlex System is a flexible rod system which has been used to preserve motion at the area of implantation. There has not been a scientific study showing how much motion is preserved after implantation. Methods : A total of 12 consecutive patients underwent posterior dynamic stabilization using the BioFlex System. Six patients were treated using a L3-4-5 construct and other six patients using a L4-5-S1 construct. Follow-up ranged from 12 to 33 months and standing neutral lateral, extension, flexion and posteroanterior (PA) radiographs were obtained at 3, 6, 9, and 12 months and at more than 12 months postoperatively. Range of motion (ROM), whole lumbar lordosis, and ROMs of motion segments from L2 to S1 were determined. Results : Patients with a L3-4-5 construct demonstrated a decrease in mean ROM for whole lumbar decreased from 40.08 to 30.77. Mean ROM for L3-4 (6.12 to 2.20) and L4-5 (6.55 to 1.67) also decreased after one year. Patients with a L4-5-S1 construct demonstrated L4-5 (8.75 to 2.70) and L5-S1 (9.97 to 3.25) decrease of mean ROM at one year postoperatively. Lumbar lordosis was preservep at both L3-4-5 and L4-5-S1 constructs. Clinical results showed significant improvements in both study groups. Conclusion : The present study provides preliminary information regarding the BioFlex motion preservation system. We conclude that the BioFlex System preserves functional motion to some degree at instrumented levels. However, although total lumbar lordosis was preserved, ROMs at implantation segments were lower than preoperative values.

Computation of Irrigation Interval and Amount as affected by Growing Substrate and Soil Depth Planted with Zoysia japonica in Green Roof during a Dry Summer (여름철 무강우 시 들잔디 옥상녹화 식재지반에 따른 관수주기 및 관수량 산정)

  • Ju, Jin-Hee;Bae, Gyu-Tae;Kim, Won-Tae;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.21 no.3
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    • pp.289-296
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    • 2012
  • The purpose of this study was to identify the irrigation intervals and the amount of suitable growing substrate needed to achieve the desired shallow-extensive green roof system during a dry summer in Korea. In terms of treatment, three types (SL, $P_6P_2L_2$, $P_4P_4L_2$) with varying soil mixture ratios and two types (15 cm, 25 cm) with varying soil depths were created. The results have been analyzed after measuring growth and soil water contents. The difference of growth by treatment was significant in terms of green coverage, height, leaf width and photosynthesis. In measurement of chlorophyll content, no difference was detected when measured against soil depth. According to the growth measurement of Zoysia japonica with respect to differing soil mixture ratios in the 15 cm-deep treatment, a statistical difference was detected at the 0.05 significance level in photosynthesis. In case of green coverage, height, chlorophyll content and leaf width, no statistical significance was observed. In case of the 25 cm-deep treatment, a statistical significance was observed in height and photosynthesis. In terms of green coverage, chlorophyll content and leaf width, no statistical significance was detected. In comparisons of soil moisture tension and soil water contents, the irrigation interval and amount were 8 days and 14.9 L in the SL (15 cm) treatment, respectively. The irrigation interval showed for 10 days a 1.3-fold increase, and the irrigation amount was 27.4 L 1.8-fold more than SL (25 cm), respectively. For $P_6P_2L_2$ (15 cm) treatment, the irrigation interval and amount were 12 days and 20.7 L, respectively. However, an irrigation interval under $P_6P_2L_2$ (25 cm) was for 15 days 1.3 times longer than $P_6P_2L_2$ (15 cm), and an irrigation amount of 40 L was 1.9 times more than that under $P_6P_2L_2$ (15 cm). In $P_4P_4L_2$ (15 cm) treatment, it was indicated that the irrigation interval was 15 days, and the irrigation amount was 19.2 L. It was not needed to irrigate for 16 days under $P_4P_4L_2$ (25 cm) treatment during the dry summer and the longest no-rain periods. The irrigation interval and amount under $P_4P_4L_2$ were 1.8-fold and 1.3-fold, respectively, more than SL treatment as affected by soil mixture ratio. Comparatively $P_4P_4L_2$ had more 1.3-fold and 0.9-fold in irrigation interval and amount more than $P_6P_2L_2$. Therefore, it can be noted that different soil depth and soil mixture ratios had a significant effect on the irrigation interval and amount.

Serological Study on Leptospirosis in Dairy Cattle (젖소의 Leptospira균에 대한 혈청학적 조사)

  • 장경진;배춘식;전학진;조용성
    • Journal of Veterinary Clinics
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    • v.14 no.1
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    • pp.1-5
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    • 1997
  • This experiment was performed to investigate the leptospiral antibody in dairy cattle with serological test in three areas of Kangwondo from February to June, 1995. Twelve different living antigen's (S icterohemorrhagiae, Lpomona, L hardjo, L australis, L canicola, L autumnalis, L grippotyphosa, L tarassovi, L pyroenes, L bataviae, L hebdomadis and L ballum) of Leptospira interrogans were used for the serological test in dairy cattle. The blood samples of 130 cattle were examinde by microscopic agglutination test. Among the serum samples of the dairy cattle, 5 heads of the dairy cattle (3.8%) were positive. Among the positive samples of 5 heads, 3 heads of dairy cattle (2.3%) showed the antibody of L canicola, 2 heads of dairy cattle (1.5%) showed the antibody of L icterohaemorrhagiae. Positive rate by age was 0% ($\leq $2year), 4.6% (3~4year) and 6.6% ($\geq $5year).

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Chemical Characteristics of Fog at a Forested Area in Jinju (진주시 주변 산림에서 안개의 화학적 특성)

  • Lee, Chong-Kyu
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.51-57
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    • 2012
  • This study was carried out to analyze chemical compositions of the fog water of a forest area, Jinju and to provide basic information for establishing measures on the acid fog water of forest area. The results are as follows: The pH of fog water was 4.3 in 2010, whereas the pH in 2011 was 4.0. The electrical conductivity of the fog water was $477.2{\mu}s$ in 2010, and $562.7{\mu}s$ in 2011. Among the anions, the concentration of $NO^{3-}$ was the highest, which recorded 267.1 mg/L in spring season and 279.1 mg/L in summer season, followed by $SO{_4}^{2-}$ at the concentration of 177.2 mg/L in spring season and 198.6mg/L in summer season. In autumn and winter, the concentration of $NO^{3-}$ was highest as 217.7 mg/L and 237.9 mg/L, respectively and followed by $SO{_4}^{2-}$, which concentration was 164.2 mg/L in autumn season and 190.1 mg/L in winter season (p<0.05). Among the cations, the concentration of $Ca^{2+}$ was 221.3 mg/L in spring and 233.7mg/L in summer, followed by $Na^+$ at 125.1 mg/L in spring season and 131.7 mg/L in summer In autumn and winter, the concentration of $Ca^{2+}$ was highest at 196.8 mg/L and 198.8 mg/L, followed by $Na^+$ at the concentration of 97.1 mg/L in autumn and 117.2 mg/L in winter (p<0.05). The pH of the fog rain that causes acid mist showed the correlation with $Ca^{++}$ (1% of level), $EC(r=-0.9861^{**})$, $NO^{3-}$ ($r=-0.9677^{**}$), and $SO{_4}^{2-}$ ($r=-0.9510^{**}$). The regression equation on the factors affecting the generation of acidic fog rain was estimated to be a $Y(pH)=6.4627+0.9723X_2(EC)+0.9364X_4(NO_3{^-})+0.9044X_5(SO{_4}^{2-})+0.8049X_{10}(Ca^{2+})+0.6709X_8(K^+)\;(r^2=0.8787)$.

The runoff characteristics of non-point source to urban stream during rainfall (강우 시 도시 하천으로의 비점오염원 유출특성)

  • Park, Woon-Ji;Kim, Dong-Oog;Ahn, Johng-Hwa;Lee, Chan-Ki
    • Journal of Industrial Technology
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    • v.27 no.B
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    • pp.83-89
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    • 2007
  • We studied runoff characteristics of combined sewer overflows in a city while it was raining. The event mean concentration (EMC) of biochemical oxygen demand (BOD), chemical oxygen demand ($COD_{Cr}$), suspended solids (SS), total nitrogen (TN), and total phosphorus (TP) in one of the combined sewer sites in Chuncheon was 63.5-211.6 mg/L, 114.9-523.8 mg/L, 70.3-436.4 mg/L, 6.4-33.0 mg/L, and 1.09-6.81 mg/L, respectively. In another combined sewer, the EMC of BOD, COD, SS, TN, and TP was 42.1-131.4 mg/L, 107.7-256.5 mg/L, 33.7-221.1 mg/L, 7.9-26.4 mg/L, and 1.16-3.91 mg/L, respectively. The ratio of the cumulative pollutant mass and the cumulative discharged volume determined using all parameters (BOD, $COD_{Cr}$, SS, TN, and TP) was over 1.0, which shows the first flush effect. Relationships between flow and loadings of BOD, $COD_{Cr}$, SS, TN, and TP were 0.90, 0.89, 0.88, 0.89, 0.92, respectively. Although the size of two areas was almost same, pollutant concentration and loading were different because of the amount of rainfall, rainfall intensity and basin area.

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Effect of Copper on the Suspended Growth Biological Wastewater Treatment (부유 성장식 생물학적 폐수처리에 미치는 구리의 영향)

  • Seo, Jeong-Beom;Hwang, Chang-Min
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.7
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    • pp.479-484
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    • 2013
  • This study was performed to examine the effect of copper on the biodegradability, nitrification, denitrification and oxygen uptake rate (OUR) using batch reactor and continuous flow stirred tank reactor (CSTR) of anaerobic/anoxic/oxic ($A_2/O$). The results of this study can be summarized as follows. In the case of the effect of copper on organic treatment, the bad effect initiated when it was above 4.5 mg/L copper with batch reactor and above 2.0 mg/L copper with CSTR. Concerning the case on nitrification and removal of nitrogen, it showed bad effect when copper was above 4.5 mg/L with batch reactor for nitrification and 1.0 mg/L with CSTR for the removal of nitrogen. The bad effect on the removal of phosphorus began when it was 4.5 mg/L copper with batch reactor and 2 mg/L copper with CSTR. In the case of OUR, it decreased as microbial activity was affected when copper concentration was above 1.5 mg/L in both case of batch reactor and CSTR.

In Vitro Morphogenesis through Leaf Explants of Gypsophila paniculata L.

  • Jo, Man-Hyun;Ham, In-Ki;Song, Nam-Hyun
    • Plant Resources
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    • v.3 no.2
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    • pp.135-137
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    • 2000
  • Callus cultures from leaf explants of Gypsophila paniculata L. cv. 'Bristol Fairy' have been tested their growth and morphogenic capacity on Murashige and Skoog medium supplemented with 0.l, 0.5, 1 and 3 mg/L 2,4-D. The frequency of callus formation ranged from 43.3% to 100%. The optimal 2,4-D concentration for promoting callus formation and growth was 0.5 to 3 ㎎/L. 4.2∼ 5.6% of adventitious roots were obtained with the use of 0.1 and 0.5 mg/L 2,4-D. Calli grown well on 1.0 mg/L 2,4-D was the heaviest among the calli grown in various concentrations.

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Cultural Condition of Pseudomonas mendocina for Polysaccharide Production (Pseudomonas mendocina 에 의한 Polysaccharide 생산)

  • Yoo, Jin-Young;Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.21 no.5
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    • pp.619-623
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    • 1989
  • The cultural condition of Pseudomonas mendocina for polysaccharide production was examined. The optimal medium contains following composition per liter of distilled water: Sucrose 23.75g, $(NH_4)_2SO_4$ 1.57g, Yeast extract 0.5g, $KH_2PO_4\;2.9g,\;MgSO_4.\;7H_2O\;1.0g,\;CaCO_3$ 2.5g. The optimum temperature and pH were $30^{\circ}C$ and 6.5. At the condition. Ps mendocina produced 5.98g/l of polysaccharide. The culture viscosity after 3 days was 191mPa.s at $70sec^{-1}$. The product yield $(Y_{p/s})$ and specific productivity $(Q_p)$ were 25.18% and 32.83mg/g-cell/h.

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