• Title/Summary/Keyword: L1 - L4

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Micropropagation through Callus Culture in Chinese Foxglove (Rehmannia glutinosa) (지황의 캘러스 배양에 의한 기내 대량증식)

  • 박충헌;성낙술;백기엽;이철희
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.3
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    • pp.171-175
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    • 1998
  • Chinese foxglove (Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the crude drug damand expands rapidly.This study was conducted to obtain the basic breeding information of Chinese foxglove. Effects of supplemental plant growth regulators were investigated on leaf tissue for proliferation. 100% callus formation, 31% plantlet regeneration and 6% root differentiation were obtained by adding 0.5 mg/L NAA and 2.0 mg/L BA. 2,4-D and Zeatin treatment also resulted in 95% increase in callus formation, but shoot was not formed. During the subculture, callus propagation rate recorded 15.4% with 0.2 mg/L NAA and 1.0 mg/L BA and plant regeneration improved on MS medium supplemented with 0.2 mg/L NAA and 0.5 mg/L kinetin. The number of shoot formed ranged from 1.7 on WPM medium to 3.4 on MS medium with 0.1 mg/L NAA and 0.5 mg/L BA. Supplementation of 1.0 g/L activated charcoal improved the In vitro plant growth.

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Correlation between Lumbar Malposition and Disc Herniation in Lumbar Disc Herniation Patients: Focused on L4-L5, L5-S1 (요추 추간판 탈출 환자에서 나타나는 요추부 변위와 추간판 탈출의 상관관계: L4-L5, L5-S1 요추 간 추간판 탈출을 중심으로)

  • Yeon-Hoo Yi;Da-woon Song;Jae-Min Jeong;Tae-ha Kwon;Sae-young Bong;Yoo-jin Lee;Jin-Bong Choi
    • Journal of Korean Medicine Rehabilitation
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    • v.33 no.4
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    • pp.185-193
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    • 2023
  • Objectives This study was conducted to find out the correlation between lumbar malposition and lumbar disc herniation. Methods Among the patients who visited Gwangju Jaseng Hospital of Korean Medicine from January 2019 to January 2021, taking 92 patients under age 60 who had the records of X-rays and magnetic resonance imaging (MRI) images as the research subjects, Cobb's angle was measured in anterior-posterior (AP) view and lateral (LAT) view, the number of displacements was scored by listing categories defined by The Korean Society of Chuna Manual Medicine for Spine and Nerves. The degree of lumbar intervertebral disc herniation was expressed as a percentage according to the method of Kato, etc., and the correlation between each factor was analyzed. Results AP curvature and MRI herniation index showed significant positive correlation in L4-L5 level but there was no significant difference in L5-S1 level. LAT curvature and MRI herniation index had no correlation in L4-L5 level, but there was a significant negative correlation in L5-S1 level. Malposition score and MRI herniation index had a significant positive correlation in L5-S1 level, whereas there was no correlation in L4-L5. Conclusions As a result of the study, AP curvature and MRI herniation index showed a significant positive tendency in L4-L5 lumbar spine, and LAT curvature and MRI herniation index showed a significant negative tendency in L5-S1 lumbar spine. Malposition score and MRI herniation index were found to have a significant positive tendency in L5-S1 lumbar spine.

Effect of Glucose Levels and N Sources in Defined Media on Fibrolytic Activity Profiles of Neocallimastix sp. YQ1 Grown on Chinese Wildrye Grass Hay or Alfalfa Hay

  • Yang, H.J.;Yue, Q.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.3
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    • pp.379-385
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    • 2011
  • Ferulic acid esterase (FAE) and acetyl esterase (AE) cleave feruloyl groups substituted at the 5'-OH group of arabinosyl residues and acetyl groups substituted at O-2/O-3 of the xylan backbone, respectively, of arabinoxylans in the cell wall of grasses. In this study, the enzyme profiles of FAE, AE and polysaccharide hydrolases of the anaerobic rumen fungus Neocallimastix sp. YQ1 grown on Chinese wildrye grass hay (CW) or alfalfa hay (AH) were investigated by two $2{\times}4$ factorial experiments, each in 10-day pure cultures. The treatments consisted of two glucose levels ($G^+$: glucose at 1.0 g/L, $G^-$: no glucose) and four N sources (N1: 1.0 g/L yeast extract, 1.0 g/L tryptone and 0.5 g/L $(NH_4)_2SO_4$; N2: 2.8 g/L yeast extract and 0.5 g/L $(NH_4)_2SO_4$; N3: 1.6 g/L tryptone and 0.5 g/L $(NH_4)_2SO_4$; N4: 1.4 g/L tryptone and 1.7 g/L yeast extract) in defined media. The optimal combinations of glucose level and N source for the fungus on CW, instead of AH, were $G^-N4$ and $G^-N3$ for maximum production of FAE and AE, respectively. Xylanase activity peaked on day 4 and day 6 for the fungus grown on CW and AH, respectively. The activities of esterases were positively correlated with those of xylanase and carboxymethyl cellulase. The fungus grown on CW exhibited a greater volatile fatty acid production than on AH with a greater release of ferulic acid from plant cell wall.

Paper-Electrophoretic Separation of Ruthenium Chloro-Complexes (전기영동에 의한 루테늄 염화착물의 분리)

  • Byung-Hun Lee;Cheon-Hwey Cho
    • Nuclear Engineering and Technology
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    • v.16 no.2
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    • pp.58-63
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    • 1984
  • Paper electrophoretic separation of octahedrally bonded (Ruc $l_{6}$ )$^{3-}$ has been carried out by using the specially designed migration apparatus. The supporting electrolyte solutions are as follows: 0.1M-HCl $O_4$, 0.05 M-HCl+0.09M-KCl, 0.1M-HCl, 5$\times$10$^{-3}$ M-NTA, 0.01M-HCl, 0.01M-HCl $O_4$, 0.01M-citric acid, 0.01M-K $H_2$P $O_4$+0.01M-N $a_2$HP $O_4$, 0.05M-borax, 0.025M-N $a_2$C $O_3$+0.025M-NaHC $O_3$, 0.01M-N $a_3$P $O_4$, 0.01M-NaOH and 0.1 M-NaOH. The (Ruc $l_{6}$ )$^{3-}$ appears in 2 to 4 peaks and is found in several chemical species such as (RuCl ($H_2O$)$_{5}$ )$^{2+}$, cis and trans (RuC $l_2$($H_2O$)$_4$)$^{1+}$, (RuC $l_3$($H_2O$)$_3$)$^{0}$ , (RuC $l_4$($H_2O$)$_2$)$^{1-}$, (RuC $l_{5}$ ($H_2O$))$^{2-}$ and (RuC $l_{6}$ )$^{3-}$. The retention value has been found to be highest in the 0.025M-N $a_2$C $O_3$+0.025M-NaHC $O_3$ electrolyte solution.n.

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Design of Dual-Band GPS Array Antenna Using In-Direct Feeding Pad (간접급전 패드를 이용한 이중 대역 GPS 배열 안테나 설계)

  • Kang, Seung-Seok;Seo, Seung-Mo;Byun, Gangil;Choo, Hosung
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.28 no.5
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    • pp.355-365
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    • 2017
  • In this paper, we propose the design of a dual-band GPS antenna using in-direct feeding pads. The antenna consists of an upper patch for the GPS L1 band, a lower patch for the GPS L2 band, and two pads on the middle layer for feeding the two radiating patches. A hybrid chip coupler with a phase difference of $90^{\circ}$ is employed at the two feeding ports for achieving a broad circular polarization (CP) bandwidth. The proposed antenna shows bore-sight gains of 3.0 dBic(L1) and 5.1 dBic(L2), and axial ratios of 3.3 dB(L1) and 0.3 dB(L2) by measurement. The active element patterns of the fabricated array with 7 elements show bore-sight gains of -0.4 dBic (L1) and -2.4 dBic(L2), respectively. It proves that the proposed antenna structure is suitable for use in GPS array applications.

Segmental Lordosis of the Spondylolytic Vertebrae in Adolescent Lumbar Spondylolysis: Differences between Bilateral L5 and L4 Spondylolysis

  • Sugawara, Kazuhiro;Iesato, Noriyuki;Katayose, Masaki
    • Asian Spine Journal
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    • v.12 no.6
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    • pp.1037-1042
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    • 2018
  • Study Design: Retrospective study. Purpose: This study aimed to investigate whether segmental lumbar hyperlordosis of the affected vertebra in patients with spondylolysis occurs only at L5 or also occurs at L4. Overview of Literature: To the best of our knowledge, increase in segmental lordosis of the spondylolytic vertebrae has only been investigated in bilateral L5 spondylolysis; it has not been examined at different levels of bilateral spondylolysis. According to the characteristics of segmental lordosis in bilateral L5 spondylolysis, patients with bilateral L4 spondylolysis may also have increased segmental lordosis of the L4 vertebra. Methods: Patients with bilateral spondylolysis of the L5 or L4 vertebra in 2013-2015 were retrospectively identified from the hospital database. Standing lateral lumbar radiographs were assessed for the angle of segmental lordosis of the L5 and L4 vertebra, sacral slope, and lumbar lordosis. The differences in segmental lordosis of the L5 and L4 vertebra, sacral slope, and lumbar lordosis were determined using non-paired Student t-test. Results: Overall, 15 cases of bilateral L4 spondylolysis and 41 cases of bilateral L5 spondylolysis satisfied the inclusion and exclusion criteria. Lordosis of the L4 vertebra was significantly greater in the bilateral L4 spondylolysis group ($24.2^{\circ}{\pm}7.0^{\circ}$) than that in the L5 spondylolysis group ($20.3^{\circ}{\pm}6.1^{\circ}$, p=0.047). Lordosis of the L5 vertebra was significantly lower in the L4 spondylolysis group ($27.7^{\circ}{\pm}8.2^{\circ}$) than that in the L5 spondylolysis group ($32.5^{\circ}{\pm}7.3^{\circ}$, p=0.040). The sacral slope and lumbar lordosis did not significantly differ between the groups. Conclusions: Adolescent patients with bilateral spondylolysis have segmental hyperlordosis of the affected vertebra not only at the L5 level but also at the L4 level.

Synthesis and Solution Chemistry of Metal Hydrides from Cationic Rhodium(I) Catalyst Precursors [(L-L)Rh(NBD)]$ClO_4 (L-L=Fe({\eta}^5-C_5H_4PBu^t\;_2)_2,\;Fe{\eta}^5-C_5H_4PPhBu^t)_2)$

  • Tae-Jeong Kim
    • Bulletin of the Korean Chemical Society
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    • v.11 no.2
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    • pp.134-139
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    • 1990
  • The hydrogenation catalyst precursors $[(L-L)Rh(NBD)]ClO_4\;(L-L=Fe({\eta}^5-C_5H_4PBu^t\;_2)_2,\;Fe({\eta}^5-C_5H_4PPhBu^t)_2$; NBD = norbornadiene) react with $H_2(1\;atm,\;30^{\circ}C$, MeOH) to yield $[(L-L)HRh({\mu}-H)_3RhH(L-L)]ClO_4$. These hydrido species are fluxional, and variable temperature NMR studies show the existence of a number of equilibria involving both fluxional and non-fluxional species. The synthesis, solution structures, and fluxional behaviors of these hydrides are described.

Adsorption of Heavy Metal onto the Extracellular Polysaccharide Produced by the Purple Nonsulfur Photosynthetic Bacteria Rhodopseudomonas sp. KH4 (홍색 비황 광합성 세균 Rhodopseudomonas sp. KH4의 Extracellular polysaccharide의 중금속 흡착)

  • Jeong, Jeong-Hwa;Seo, Pil-Soo;Kong, Sung-Ho;Lee, Jong-Yeol;Lee, Sang-Seob
    • Korean Journal of Microbiology
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    • v.42 no.4
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    • pp.326-331
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    • 2006
  • In the present study, we examined biosorption characteristics of heavy metals onto the extracellular polysaccharide (EPS) produced by the purple nonsulfur photosynthetic bacteria Rhodopseudomonas sp. KH4, which was isolated from a stream in Anyang, Kyonggi-Do. When Cd (100 mg/L) and Cu (100 mg/L) were added to EPS (1.0 g/L) in the optimal condition (Cd; pH 8, Cu; pH 5, $40^{\circ}C$), 84.2 mg/L of Cd and 70.0 mg/L of Cu were adsorbed within 30 min and 10 min, respectively. When 100 mg/L of Cd and Cu were present as mixture, 16.8 mg/L of Cd and 48.7 mg/L of Cu were adsorbed at $25^{\circ}C$, pH 5. The maximum adsorption capacity determined by fitting Langmuir isotherms model was suitable for describing the biosorption of Cd (76.9 mg/g) and Cu (67.1 mg/g) by EPS. The neutral monosaccharide in the EPS determined by GC consisted of arabinose (2.4%), glucose (7.1%) and mannose (90.5%).

Chemical Characteristics of Ground Water for Hydroponics and Waste Nutrient Solution after Hydroponics in Chungbuk Area (충북지역 양액 재배용 지하수 및 폐양액의 화학적 특징)

  • Lee, Gyeong-Ja;Kang, Bo-Goo;Lee, Ki-Yeol;Yun, Tae;Park, Seong-Gyu;Lee, Cheol-Hee
    • Korean Journal of Environmental Agriculture
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    • v.26 no.1
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    • pp.42-48
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    • 2007
  • This survey has been conducted to obtain basic data of the quality of ground water for hydroponics and waste nutrient solution after hydroponics in hydroponic farms in Chungbuk area. Ground water samples were collected and analyzed at 19 sites of hydroponic farms. Waste nutrient solution samples were analyzed at 15 sites selected of them. The values of several components in ground water for hydroponics were as follows. pH range was shown from 6.2 to 7.7 and the average was 6.8. EC range was shown from 0.10 to 0.45 dS $m^{-1}$ and the average 0.23 dS $m^{-1}$. $NO_3-N$ concentrations was ranged from 0.12 to 13.77 mg $L^{-1}$, $SO_4^{2-}$ concentrations was ranged from 1.84 to 63.01 mg $L^{-1}$ and $Cl^-$ concentrations were ranged from 10.46 to 72.09 mg $L^{-1}$. Average values of $NO_3-N$, $SO_4^{2-}$ and $Cl^-$ were 4.00 mg $L^{-1}$, 12.70 mg $L^{-1}$ and 27.57 mg $L^{-1}$, respectively. $Ca^{2+}$, $Mg^{2+}$ and $Na^+$ concentrations were ranged from 3.24 to 36.99 mg $L^{-1}$, 1.44 to 14.93 mg $L^{-1}$ and 6.12 to 25.25 mg $L^{-1}$, respectively. Average concentrations were 13.06 mg $L^{-1}$ in $Ca^{2+}$, 6.02 mg $L^{-1}$ $Mg^{2+}$ and 12.08 mg $L^{-1}$ in $Na^+$. In waste nutrient solution after hydroponics, pH range was shown from 4.3 to 8.8 and the average was 6.7. EC range was shown from 0.44 to 2.37 dS $m^{-1}$ and the average 1.15 dS $m^{-1}$. Range of $NO_3-N$, $PO_4-P$, $K^+$, $Ca^{2+}$, $Mg^{2+}$ and $Na^+$ in waste nutrient solution were $10{\sim}212$, $0.56{\sim}26.1$, $10{\sim}295$, $16{\sim}215$, $9{\sim}54$ and $10{\sim}53$ mg $L^{-1}$ respectively. Average concentration were 100 mg $L^{-1}$ in $NO_3-N$, 12.15 mg $L^{-1}$ in $PO_4-P$, 99 mg $L^{-1}$ in $K^+$, 78 mg $L^{-1}$ in $Ca^{2+}$, 26 mg $L^{-1}$ in $Mg^{2+}$ and 26 mg $L^{-1}$ in $Na^+$. Inorganic matters in waste nutrient solution after hydroponics was higher than that of ground water for hydroponics.

수돗물 중 강력한 돌연변이유발물질인 MX에 대한 분석

  • 권오영;김희갑
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 2002.10a
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    • pp.153-153
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    • 2002
  • 수돗물의 염소소독 과정에서 부식질의 초기 분해생성물인 MX(Mutagen X)는 수돗물의 전체 돌연변이유발성의 최고 60%까지 차지하는 것으로 알려져 있다. 그러나 존재하는 농도는 수 ng/L로 정량분석을 위해서는 resin을 사용하여 10 ~ 20L의 물을 농축하여야 한다. 이 연구에서 MX의 농축에 사용된 resin은 XAD-4와 XAD-7HP의 1:1 mixture이었으며, 사용 전에 Soxhlet extractor를 사용하여 ethyl acetate와 methanol로 각각 12시간씩 세척하였다. 15L의 물 시료는 37%의 염산으로 pH 2에 맞춘 후 직경 2cm의 유리관에 15cm의 높이로 채워진 resin을 40mL/min의 유속으로 통과시켰다. 유리관에 잔류하는 물은 질소가스로 대부분 불어 제거한 후 ethyl acetate 200mL를 가해 1mL/min의 유속으로 흘려주어 MX를 용출시킨 후 회전증발기로 5mL의 부피까지 농축시키고 10% H$_2$SO$_4$ in MeOH 0.1mL를 가하여 6$0^{\circ}C$에서 methyl ester화하였다. 과포화된 $Na_2$SO$_4$ 수용액 0.3ml를 가한 후 MTBE 0.3mL로 추출하고 GC/ECD로 분석하였다. MX의 회수율은 60% 이상이었으며 일부수돗물 시료에 대해 분석하였다.

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