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Effect of Watering on Growth and Essential Oil Content of Sweet Basil (Ocimum basilicum) (관수량이 sweet basil(Ocimum basilicum)의 생장과 정유함량에 미치는 영향)

  • Baeck, Hae-Won;Park, Kuen-Woo
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.81-86
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    • 2001
  • This experiment was carried out to investigate watering with relation to growth, quality and yield of essential oil in sweet basil. The degree of water stress was taken as amount of watering. D1 was watered with 25mL for 2 weeks and 30mL from 4 to 5 weeks after planting in Wagner pot (1/20000a, ${\phi}24cm$). As this, 75mL and 90mL, 225mL and 270mL, and 675mL and 810mL were watered in D2, D3, and D4 treatment respectively. DFT was set up as water stress was not inflicted. The growth of basil in D3 and D4 was better than that of others, in which root activity was as much twice as that of D1. Essential oil of D1 was recorded the lowest content as 0.33%. The result of proline content, peroxidase activity, photosynthesis, stomatal conductance and stomatal resistance were proved D1 to be stressed. This treatment consequently increased the content of essential oil. In consideration of growth and essential oil content, D3 treatment was highest as 47.37mg in oil production per plant. Finally, D3 watered with 225mL for 2 weeks and 275mL from 4 to 5 weeks after planting could be selected on the purpose of both plant growth and essential oil production. Essential oil content of sweet basil was increased in response to water stress. For increase of essential oil yield, oil synthesis could be raised by giving water stress just before harvesting.

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Serum Fat Soluble Vitamins in Bile Duct Ligated Rats (담도 결찰한 백서에 있어서 담즙산 및 UDCA 투여에 따른 혈중 지용성 비타민의 농도 변화에 관한 연구)

  • Sim, Jay-Geon;O, Myung-Ho;Kim, Kee-Hyuck
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.2 no.1
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    • pp.59-64
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    • 1999
  • Purpose: The aims of this study are to measure the serum levels of fat soluble vitamins (vitamin A and D) from bile duct ligated rats, and to evaluate the effect of oral bile acids administration to facilitate absorption of fat soluble vitamins. Methods: We measured serum ALT, total bilirubin, vitamin A, and vitamin D of Sprague-Dawley rats 1 week before and 4 weeks after experimental bile duct ligation. Rats were consisted with 3 groups. Group 2 had been fed bile acids and group 3 ursodeoxycholic acid after operation for 4 weeks. Multi-vitamin was given to all groups. Results: 1) Base line (mean value before duct ligation): ALT 74.2 IU, total bilirubin 0.26 mg/dL; vitamin D 13.01 ng/mL vitamin A $0.87\;{\mu}g/mL$, total bile acids $25.16\;{\mu}mol/L$. 2) Four weeks after ligation: ALT 100.7 IU, total bilirubin 2.58 mg/dL; vitamin D 7.89 ng/mL vitamin A $1.37{\mu}g/mL$, total bile acids $278.22\;{\mu}mol/L$. 3) 4 weeks after ligation, each group (group 1, group 2 and group 3) showed vitamin D (7.62, 8.10 and 7.99) ng/mL, vitamin A (1.68, 1.06 and 1.33) ${\mu}g/mL$, total bile acids (233.17, 345.80 and 268.57) ${\mu}mol/L$, which were statistically not significant. Conclusion: Serum level of vitamin A is increased after bile duct ligation although vitamin D is decreased. Oral administration of bile acids does not affect the serum levels of vitamin A and D in bile duct ligated rats.

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Physicochemical Property and Antioxidant Activity of Wild Grape(Vitis coignetiea) Juice (머루 주스의 이화학적 특성 및 황산화 활성)

  • Park, Hyun-Sil
    • Culinary science and hospitality research
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    • v.16 no.4
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    • pp.297-304
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    • 2010
  • This study examines the physicochemical property and antioxidant activity of wild grape juice, and the result is as follows. Total anthocyanin and color intensity contents of wild grape juice were $17.0{\pm}0.1%$ and $33.9{\pm}1.4%$, respectively. The contents of total phenols and flavonoid in wild grape juice were $25.4{\pm}3.1$ mg/100g, $4.4{\pm}1.4$ mg/100 g, respectively. Total mineral content in wild grape juice was $28.5{\pm}8.4$ mg/l00 g and the potassium content($11.3{\pm}0.3$ mg/100 g) was the highest Electron donating abilities of wild grape juice at concentration of $1,000\;{\mu}L$/mL were $94.8{\pm}1.2%$ Reducing power of wild grape juice at concentration of $1,000\;{\mu}L$/mL was 1.134. The electron donating abilities and reducing power were increased significantly with the sample concentration in the reaction mixture increased The nitrite scavenging ability was dependent on pH of the reaction mixture and sample concentration. The nitrite scavenging ability of wild grape juice was $77.1{\pm}1.1%$ at concentration of $1,000\;{\mu}L$/mL under pH 1.2.

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Continuous Alcohol Fermentation by a Flocculating Yeast (응집성 효모를 이용한 연속 알코올 발효)

  • 남기두;이인기;조훈호
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.487-490
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    • 1991
  • In this study continuous alcohol fermentation of molasses by the recirculation system has investigated. After cultivation of yeast cells in the YPD medium with increasing the medium concentration from 10 to 183.5 g/l stepwisely, the fermentation medium was replaced by molasses. The maximum cell mass was 25 g/l, and the mean cell mass during the operation was 23.5g/1, which was 3.4 times higher compared with a conventional batch system. The optimum fermentation conditions with feeding molasses of 180 g/l were obtained when the fermentation was carried out at 500 rpm and at the dilution rate of 0.037 $h^{-1}$. Under these conditions we could safely operate the fermentor for 645 h without any trouble. The maximum alcohol productivity was 4.9 g$l\cdot h$ with an alcohol concentration of 53.9 g/l at the dilution rate of 0.091$h^{-1}$.

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Effect of Medium Component on Plant Regeneration via Adventitious Bud Formation from Leaf Explant Cultures of Strawberry (Fragaria ananassa Duch.) (딸기 (Fragaria ananassa Duch.) 잎 절편체 배양으로부터 부정아 형성을 통한 식물체 재생)

  • 조덕이;소웅영;정원일
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.3
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    • pp.171-178
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    • 2001
  • This study was investigated to establish a regeneration system of plant via adventitious bud formation from leaf explant cultures of strawberry (Fragaria ananassa Duch). Effects of plant growth regulators (2,4-D, BAP), agar sucrose and myo-inositol on adventitious bud formation were investigated. When the leaf explants were cultured on MS medium supplemented with 0.1 mg/L 2,4-D and 3 mg/L BAP, the adventitious bud formation was most promoted. The adventitious bud formation was not induced from leaf explants cultured on MS medium containing 2,4-D alone. Adventitious bud formation was enhanced to almost 3 times on medium with low level of agar concentration (0.4%) in comparison with those on the medium with high level of agar (1%), but almost of shoot was vitrificated on the medium. Therefore, the normal adventitious bud formation from leaf explants was most effective on the medium containing 0.05 mg/L 2,4-D,1 mg/L BAP, 0.8% agar, 30 g/L sucrose and 100 mg/L myo-inositol. Thus, the mass propagation of healthy strawberry could be established using leaf explants.

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Antibacterial activity of Chamaecyparis obtuse Extract and Profile of Antimicrobial Agents Resistance for Methicillin-Resistant Staphylococcus aureus

  • Jong Hwa Yum
    • Biomedical Science Letters
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    • v.30 no.1
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    • pp.32-35
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    • 2024
  • In vitro antimicrobial activities of hot water extracts of Chamaecyparis obtuse, for methicillin-resistant Staphylococcus aureus (MRSA) was compared to commonly used conventional antimicrobial agents. All MRSA was susceptible to linezolid or vancomycin, but also to erythromycin. MIC range and MIC90 to erythromycin, clindamycin, levofloxacin, tetracycline for MRSA were each 4 ㎍/mL, 2 ~ >128 ㎍/mL, ≤0.06 ~ >128 ㎍/mL, 0.25 ~ >128 ㎍/mL, 0.25~64 ㎍/mL and 4 ㎍/mL, .128 ㎍/mL, >128 ㎍/mL, >128 ㎍/mL, 64 ㎍/mL. The hot water extracts of leaf of C. obtuse had the lowest MIC range, MIC50, and MIC90 (0.125 µL/mL) for the MRSA tested, and it was possible more potent than various conventional antimicrobial agents. Screen antibacterial drug candidate with high antibacterial activity such as derivatives of C. obtuse leaf extract such as terpinen-4-ol or using combined therapy with commercialized antibacterial agents will likely be helpful in treating refractory MRSA infections.

A Study on Correlation of the Results VFA Measured by CT Position with VFA Measured by InBody (CT 측정 위치에 따른 내장지방 면적과 Inbody로 측정한 내장지방 면적의 상관성 연구)

  • Lee, Sang Heon;Im, In Chul;Lee, Hyo-Yeong
    • Journal of the Korean Society of Radiology
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    • v.13 no.5
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    • pp.691-698
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    • 2019
  • The patients who visited the Health Promotion Center were compared between the visceral fat area according to CT fat measurement position and the visceral fat area measured by Inbody. In the CT measurement, the visceral fat area measured at the L4-5 and CT Umbilicus positions was not different regardless of gender. In addition, there was no difference between CT visceral fat area and Inbody visceral fat area in the correlation between visceral fat area according to CT measurement position and visceral fat area measured by Inbody. The highly correlated CT measurement position were male L4-5, L5-S1, female L3-4, L4-5, L5-S1, and Umbilicus. In addition, when studying the relationship between the inbody visceral fat area and CT visceral fat area regardless of gender, it is suggested to compare the visceral fat area at the CT L4-5 position.

Effect of Plant Growth Regulators on Bulblet Formation and Plant Regeneration in Fritillaria thunbergii Miq. (패모조직배양에서 생장조절 물질이 자구형성 및 식물체 재생에 미치는 영향)

  • Park, Chul-Hyoung;Ryu, Jeom-Ho;Han, Kwang-Soo;Doo, Hong-Soo;Choi, Sun-Young
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.2
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    • pp.119-125
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    • 1996
  • To improve the efficiency of mass propagation in vitro of Fritillaria thunbergii, bulb scale and nodes were cultured in LS medium supplemented with the combination of 2, 4-D and kinetin or NAA and BA. The number and size of bulb, the number of adventitious shoot, the ratio of callus formation, rooting, and the effects of light and dark on the culture, plant regeneration from calli, and the gelling substances were investigated. The combination of 2, 4-D and kinetin in media was more effective than the media of NAA and BA for the bulblet formation. The media supplemented with 2 mg/L 2, 4-D and 1 mg/L kinetin, $1{\sim}2\;mg/L$ 2, 4-D without kinetin and $1{\sim}3\;mg/L$ BAA only were effective in the adventitious shoot development. Callus formation and root formation, respectively were effective in the medium supplemented with 2mg/L 2, 4-D and 1mg/L kinetin. In bulb formation, the medium with 5 mg/L kinetin was effective, and the most of bulbs were formed from the axillary bud of node part. In bulb formation, shoot growth, callus and root formation, the light culture for 16 hours per day was better than that in the dark culture. Bulb was nicely formed in the medium with 0. 2 mg/L 2, 4-D, 1 mg/L kinetin. The medium without hormone was most effective for plant regeneration. The phytagel was more effective than agar in the medium as the gelling agent.

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Effects of Explant Parts and Plant Growth Regulators on the in vitro Propagation of Lycoris squamigera (상사화의 기내증식에 미치는 배양부위와 생장조절물질의 영향)

  • Eun, Jong-Seon;Kim, Young-Seon;Park, Jong-Suk;JIN, Song Nan;CAO, Hounan
    • Journal of Plant Biotechnology
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    • v.29 no.3
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    • pp.179-183
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    • 2002
  • This study was carried out to investigate the influence of medium composition for in vitro mass propagation of Lycoris squamigera Max. After the disks of short stems, segments of leaf within bulb and scale were cultured on MS basal medium supplemented with various plant growth regulators, they were examined for the extent of callus formation, shoot and root regeneration. In the culture of stem disks, adventitious shoots were regenerated from the basal tissue of bulb scales, and combined medium of 1.0 mg/L 2,4-D or NAA+2.0 mg/L BA or kinetin showed the the best response and 4∼6 shoots per explant formed. In the culture of leaf segments within bulbs, both MS medium supplemented with 1.0 mg/L NAA+2.0 mg/L TDZ and with 1.0 mg/L 2,4-D+1.0∼2.0 mg/L BA were produced callus profusely on the base of leaf tissue and 3∼6 shoots were regenerated per explant. In the scale segments culture, calli were produced on the basal tissue on medium with 1.0 mg/L 2,4-D+1.0∼2.0 mg/L BA. The best result were shown on MS medium with 1.0 mg/L NAA+2.0 mg/L TDZ, and 1.0 mg/L 2,4-D+1.0∼2.0 mg/L BA. Maximum number of regenerated shoots was up to 10∼12. Adventitious root formation from explants were formed profusely on MS medium with 1.0 mg/L NAA+2.0 mg/L kinetin. The most desirable method for mass propagation of plantlets was the shoot regeneration from scale segments then subsequently subcultured on medium for rooting.

The Effect of the Interactive Flow on Convective Heat Transfer from two Vertical Isothermal Parallel Plates (수직 등온 평행 평판에서 상호작용 유동이 대류 열전달에 미치는 영향)

  • 김상영;정한식;권순석
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.16 no.4
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    • pp.765-774
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    • 1992
  • The effect of the interactive flow on convective heat transfer from two vertical isothermal parallel plates have been studied numerically by the finite difference method. The Reynolds number, Grashof number, the relative length, L$_{2}$/L$_{1}$, and the dimensionless plate spacing, b/L$_{1}$ are varied as parameters. In case of outside mean Nusselt number, left outside mean Nusselt numbers show same values as L$_{2}$/L$_{1}$ and b/L$_{1}$ increase, but right outside mean Nusselt numbers decrease as L$_{2}$/L$_{1}$ increases. The inside mean Nusselt numbers are constant at narrow spacings and increase at wide spacings as Grashof numbers increase. The optimun plate spacing on left inside mean Nusselt numbers is b/L$_{1}$=0.4 at Re=100 and b/L$_{1}$=0.3 at Re=200. For the right inside mean Nusselt number, the optimum plate spacings move to the narrow spacing as Reynolds numbers increase and L$_{2}$/L$_{1}$ decrease.