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http://dx.doi.org/10.5010/JPB.2002.29.3.179

Effects of Explant Parts and Plant Growth Regulators on the in vitro Propagation of Lycoris squamigera  

Eun, Jong-Seon (Research Institute of Bioindustry, Chonbuk National University)
Kim, Young-Seon (Dept. of Horticultural Industry, Namdo Provincial College of Jeonnam)
Park, Jong-Suk (Research Institute of Bioindustry, Chonbuk National University)
JIN, Song Nan (Research Institute of Bioindustry, Chonbuk National University)
CAO, Hounan (Horticultural Dept., Agricultural College of Yanbian University)
Publication Information
Journal of Plant Biotechnology / v.29, no.3, 2002 , pp. 179-183 More about this Journal
Abstract
This study was carried out to investigate the influence of medium composition for in vitro mass propagation of Lycoris squamigera Max. After the disks of short stems, segments of leaf within bulb and scale were cultured on MS basal medium supplemented with various plant growth regulators, they were examined for the extent of callus formation, shoot and root regeneration. In the culture of stem disks, adventitious shoots were regenerated from the basal tissue of bulb scales, and combined medium of 1.0 mg/L 2,4-D or NAA＋2.0 mg/L BA or kinetin showed the the best response and 4∼6 shoots per explant formed. In the culture of leaf segments within bulbs, both MS medium supplemented with 1.0 mg/L NAA＋2.0 mg/L TDZ and with 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA were produced callus profusely on the base of leaf tissue and 3∼6 shoots were regenerated per explant. In the scale segments culture, calli were produced on the basal tissue on medium with 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA. The best result were shown on MS medium with 1.0 mg/L NAA＋2.0 mg/L TDZ, and 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA. Maximum number of regenerated shoots was up to 10∼12. Adventitious root formation from explants were formed profusely on MS medium with 1.0 mg/L NAA＋2.0 mg/L kinetin. The most desirable method for mass propagation of plantlets was the shoot regeneration from scale segments then subsequently subcultured on medium for rooting.
Keywords
Bulb; mass propagation; regeneration; scale segment;
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