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Protective Effect of Radiation-induced New Blackberry Mutant γ-B201 on H2O2-induced Oxidative Damage in HepG2 Cells (H2O2 에 의해 유도된 HepG2 세포의 산화적 스트레스에 대한 신품종 방사선 돌연변이 블랙베리 γ-B201의 세포 보호 효과)

  • Cho, Byoung Ok;Lee, Chang-Wook;So, Yangkang;Jin, Chang-Hyun;Yook, Hong-Sun;Byun, Myung-Woo;Jeong, Yong-Wook;Park, Jong Chun;Jeong, Il-Yun
    • Korean Journal of Food Science and Technology
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    • v.46 no.3
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    • pp.384-389
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    • 2014
  • The objective of the present study was to investigate the chemical composition of anthocyanin-enriched extract of radiation-induced blackberry (Rubus fruticosus L.) mutant (${\gamma}$-B201) as well as the protective effect of ${\gamma}$-B201 against oxidative stress in vitro. The cytotoxicity, reactive oxygen species (ROS) scavenging capacity, and DNA damage were assessed by WST-1 assay, flow cytometry, and comet assay, respectively. Lactate dehydrogenase, superoxide dismutase, and catalase activities were determined by using a commercial kit. The in vitro results showed that ${\gamma}$-B201 increased the cell viability, reduction of lactate dehydrogenase release, and intracellular ROS scavenging capacity in hydrogen peroxide ($H_2O_2$)-treated HepG2 cells. Furthermore, treatment with ${\gamma}$-B201 attenuated DNA damage in $H_2O_2$-treated HepG2 cells and treatment with ${\gamma}$-B201 restored the activity of superoxide dismutase and catalase in $H_2O_2$-treated HepG2 cells. In conclusion, the present study suggests that ${\gamma}$-B201 blackberry extract can exert a significant cytoprotective effect against $H_2O_2$-induced cell damage.

Comparison of antioxidant activities and effective compounds in Korean and Chinese Torreya seeds (한국산 및 중국산 비자 열매의 항산화 활성과 유효성분 비교)

  • Kim, Saet Byul;Kim, Byung Woo;Hyun, Sook Kyung
    • Korean Journal of Food Science and Technology
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    • v.50 no.3
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    • pp.274-279
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    • 2018
  • To evaluate the antioxidant activities of Korean and Chinese Torreya seeds, their total phenolic compound content, total flavonoid content, DPPH radical and ONOO-scavenging activities were compared using their water and methanol extracts. The effective compounds were identified and quantitatively analyzed by GC-MS. The DPPH and ONOO-scavenging activities were the highest in the Korean Torreya seeds. After using GC-MS to identify the active compounds, a total of eight compounds were identified in Korean Torreya seeds, and five compounds were found in Chinese Torreya seeds. In conclusion, we could confirm the antioxidant activity and the difference between active compounds of the Korean and Chinese Torreya seeds; we also confirmed the superiority of Korean Torreya seeds. Futhermore, this basic data about the Korean and Chinese Torreya seeds can be provided to consumers, so that they can select proper and suitable functional foods.

Dispersal of sugar beet cyst nematode (Heterodera schachtii) by water and soil in highland Chinese cabbage fields (고랭지 배추 재배지에서 사탕무씨스트선충의 물과 토양을 통한 포장 간 분산)

  • Kwon, Oh-Gyeong;Shin, Jin-Hee;Kabir, F. Md.;Lee, Jae-Kook;Lee, DongWoon
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.195-205
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    • 2016
  • Sugar beet cyst nematode, Heterodera schachtii, causes serious economic losses worldwide in Brassicaceae crops. In 2011, this nematode was first found in highland vegetable cultivation areas in Korea, and thereafter spread to the surrounding healthy Chinese cabbage fields. However, little has been documented on the biological and ecological characteristics of the sugar beet nematode in highland vegetable cultivation areas. In this study the dispersal of the sugar beet cyst nematode was examined, focusing on spreading through soil and/or water infested with the nematode. When farmers with work boots trampled on Chinese cabbage fields for 10 minutes, the number of cysts recovered from the soil attached to the working differed depending on the research sites. Under irrigation conditions of 2, 4, and 8 liters of water per $m^2$, the amounts of soils attached on the bottom of the work boots and the number of healthy cysts in the soils increased significantly with the increase in soil moisture, although the total number of cysts, eggs, and juveniles did not increase significantly. After driving on the farm road adjacent to cabbage fields infested with the sugar beet cyst nematode, cysts were also recovered from the soil attached to the vehicle's tires, suggesting that the sugar beet cyst nematode can spread to new fields through soil carried by vehicles and by farmers. An excavator and a motor truck could deliver 41 kg and 224 g, respectively, of soil on the shovel shoes and the wheels to other locations during the Chinese cabbage harvest, suggesting that farming equipment and transportation vehicles may be vital means by which the cyst nematode spreads to non-infested fields in the highland area of Korea. Runoff water also contained cysts, whose amounts increased after water irrigation onto the ridges to simulate rainy conditions, with no significant difference in the number of cysts with increasing amounts of irrigation water. All of these results confirmed that the sugar beet cyst nematode spreads through soil attached to work boots, harvesting tools, and transportation vehicles, especially during the harvest time, and through runoff water on rainy days. These observations suggest that personal sanitization and cleaning of working tools and vehicles are one of the most important means to prevent the dispersal of the sugar beet cyst nematode in fields.

Effect of Maturity Scores and Number of Extractions on the Chemical Properties of Water Extract from Hanwoo Shank Bones (한우 성숙도와 추출횟수가 사골용출액의 화학적 특성에 미치는 영향)

  • Kim, Jin-Hyoung;Cho, Soo-Hyun;Seong, Pil-Nam;Hah, Kyung-Hee;Jeong, Jin-Hyung;Lim, Dong-Gyun;Park, Beom-Young;Lee, Jong-Moon;Kim, Dong-Hun;Ahn, Chong-Nam
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.463-468
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    • 2007
  • This study was conducted to investigate the effect of maturity scores [2 (bull), 2 (steer), 3-9 (cow)] and the number of extractions (up to 4 times) on the chemical properties of water extract from Hanwoo shank bones (arm, fore shank, round and hind shank). The turbidity, meat color (CIE L value), collagen, protein, caloric and chondroitin sulfate contents of samples were observed. The turbidity and lightness were higher for water extract of Hanwoo shank bones with a maturity score of 2 (bull and steer) than maturity scores of 3-9 (cow) (p<0.05). The turbidity and lightness of water extract from shank bones of all Hanwoo maturity scores significantly increased with the 1st and 2nd extractions, but significantly decreased with 3rd and 4th extractions (p<0.05). The collagen and protein contents were highest for water extract from Hanwoo shank bones of maturity score 2 (bull and steer) (p<0.05). The caloric and chondroitin sulfate contents were higher for water extract from Hanwoo shank bones of maturity score 2 (bull and steer) than maturity scores of 3-9 (cow) (p<0.05). As the number of extractions increased, the chondroitin sulfate content significantly decreased (p<0.05). Based on these results, differences correlating with maturity scores were found only with collagen and protein contents. Therefore, further studies should be considered to address whether different maturity scores affect the price of shank bones in the meat industry.

Precise, Real-time Measurement of the Fresh Weight of Lettuce with Growth Stage in a Plant Factory using a Nutrient Film Technique (NFT 수경재배 방식의 식물공장에서 생육단계별 실시간 작물 생체중 정밀 측정 방법)

  • Kim, Ji-Soo;Kang, Woo Hyun;Ahn, Tae In;Shin, Jong Hwa;Son, Jung Eek
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.77-83
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    • 2016
  • The measurement of total fresh weight of plants provides an essential indicator of crop growth for monitoring production. To measure fresh weight without damaging the vegetation, image-based methods have been developed, but they have limitations. In addition, the total plant fresh weight is difficult to measure directly in hydroponic cultivation systems because of the amount of nutrient solution. This study aimed to develop a real-time, precise method to measure the total fresh weight of Romaine lettuce (Lactuca sativa L. cv. Asia Heuk Romaine) with growth stage in a plant factory using a nutrient film technique. The total weight of the channel, amount of residual nutrient solution in the channel, and fresh shoot and root weights of the plants were measured every 7 days after transplanting. The initial weight of the channel during nutrient solution supply (Wi) and its weight change per second just after the nutrient solution supply stopped were also measured. When no more draining occurred, the final weight of the channel (Ws) and the amount of residual nutrient solution in the channel were measured. The time constant (${\tau}$) was calculated by considering the transient values of Wi and Ws. The relationship of Wi, Ws, ${\tau}$, and fresh weight was quantitatively analyzed. After the nutrient solution supply stopped, the change in the channel weight exponentially decreased. The nutrient solution in the channel slowly drained as the root weight in the channel increased. Large differences were observed between the actual fresh weight of the plant and the predicted value because the channel included residual nutrient solution. These differences were difficult to predict with growth stage but a model with the time constant showed the highest accuracy. The real-time fresh weight could be calculated from Wi, Ws, and ${\tau}$ with growth stage.

Peptide Properties of Rapid Salted and Fermented Anchovy Sauce Using Various Pretenses 2. Characterization of Hydrolytic Peptides from Anchovy Sauce and Actomyosin (단백질 분해효소를 이용하여 제조한 속성 멸치 액젓의 펩티드 특성 2. 멸치 액젓 및 Actomyosin의 가수분해 펩티드의 특성)

  • CHOI Yeung-Joon;KIM In-Soo;CHO Young-Je;SEO Duck-Hoon;LEE Tae-Gee;PARK Yeung-Beom;PARK Jae-Woon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.4
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    • pp.488-494
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    • 1999
  • Hydrolytic peptides of salted and fermented anchovy sauce, and anchovy actomyosin for the development of a rapid fermentation method with conventional tastes and flavors were studied. The optimal temperatures of crude enzymes isolated from anchor, liver and viscera of squid were 55, 40$\~$45 and $45\~60^{\circ}C$, respectively. Crude enzyme isolated from anchovy was more effective on hydrolysis of anchovy actomyosin than that from squid liver and viscera. But the crude enzyme from squid liver was less effective on NaCl than that from anchovy. Three peptides occurred in anchovy actomyosin hydrolyzed with crude enzymes from anchovy and squid liver for 30 min. Their molecular weight were determined by Superdex 200 gel chromatography as 10,800, 5,800 and 2,600 dalton. When anchovy sauce was hydrolyzed with crude enzymes of anchovy, squid liver and viscera, and Protamex during 70 days, ranges of their low molecular weight of hydrolyzed peptides were 300$\~$1,000dalton detected by Sephadex G-50 and their major amino acid compositions were glutamic acid, glycine and alanine, which was related with conventional tastes. Those amino acid compositions were similar to those of anchovy sauce hydrolyzed with squid liver, In the case of Protamex treatment, hydrolyzed peptides had high levels of isoleucine and leucine, being associated with the bitter, but a low level of glutamic acid.

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Effects of Artificial Acid Precipitation on Forest Soil Buffer Capacities (인공산성우(人工酸性雨)가 삼림토양(森林土壤)의 완충능(緩衝能)에 미치는 영향(影響))

  • Min, Ell Sik;Lee, Soo Wook
    • Journal of Korean Society of Forest Science
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    • v.79 no.4
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    • pp.376-387
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    • 1990
  • A research effort has been made to determine soil buffer capacity in forest soils nearby urban and industrialized regions. Buffer capacities of soils from four regions were measured by different pH levels of artificial acid precipitation. The following conclusions have been drawn in response to the overall research objectives. Soil Suffer capacity was the highest in Kangwondo followed by Uisan, Yeochon and Seoul when simulated acid precipitation were treated at the level of pH 3.0-5.7. With the acid precipitation treatment below pH 2.0 level, however, the capacity dropped seriously with no significant differences between the regions. In Kangwondo region soils weathered from granite and limestone showed significant differences in the buffer capacities. Soil collected in Seoul and Ulsean revealed that the capacities tended to increase with the distance from the pollution sources when treated at pH 3.0, 4.5 and 5.7 level of acid precipitation. The major mechanism of soil buffer observed during simulated acid precipitation experiment was canon exchange for Kangwondo forest soils. In Seoul region canon exchange also played an important role in soil buffering under artificial acid precipitation between 3.0 and 5.7 pH levels, yet under pH 2.0 level aluminum and silicate hydrolysis. In Ulsan canon exchange was a msjor determinant for the buffer capacity above pH 4.5 level, between pH 3.0-4.5 aluminum hydrolysis and below pH 3.0 aluminum and silicate hydrolysis. In Yeochon silicate hydrolysis led buffer capacity above pH 4.5 and below pH 4.5 aluminum hydrolysis.

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A New High Qualilty Rice Variety with High Head Rice Ratio and Milling Recovery, "Chilbo" (완전미율, 도정수율 높은 중만생 고품질 벼 신품종 "칠보(七寶)")

  • Kim, Jeong-Il;Chang, Jae-Ki;Park, No-Bong;Yeo, Un-Sang;Oh, Byeong-Geun;Kang, Jung-Hun;Kwon, Oh-Deog;Shin, Mun-Sik;Park, Dong-Soo;Kwak, Do-Yeon;Lee, Jong-Hee;Song, You-Cheon;Kim, Chun-Song;Cho, Jun-Hyun;Yi, Gihwan;Lee, Ji-Yoon;Nam, Min-Hee;Kim, Sang-Yeol;Ahn, Jong-Woong;Ku, Yeon-chung;Kim, Jae-Kyu
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.547-551
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    • 2009
  • A new commercial rice variety "Chilbo" is a japonica rice (Oryza sativa L.) with resistance to rice stripe virus and high grain quality. It was developed by the rice breeding team of Yeongdeog Substation, NICS, RDA in 2007. This variety was derived from a cross between "Yeongdeog 26" with wind tolerance and lodging resistance and "Koshihikari" with good grain quality. A promising line, YR21324-119-3-2 was selected by a pedigree breeding method and designated as "Yeongdeog 44" in 2004. Regional adaptation yield trials were carried out at eleven locations from 2005 to 2007. As a result, "Yeongdeog 44" was released as a high yielding rice variety with high grain qualilty and virus resistance with the name of "Chilbo". It is short 76cm in culm length and has medium-late growth duration. This variety is resistant to stripe virus and middle resistant to leaf blast disease. It is also tolerant to cold, dried wind. Milled rice kernel of "Chilbo" is translucent, clear in chalkiness. Panel test proved that and it has good eating quality. Head rice ratio of Chilbo is high compared to the check variety, Hwaseongbyeo. Yield potential of "Chilbo" in milled rice is about 5.57MT/ha at ordinary fertilizer level of local adaptability test. This variety would be adaptable to Yeongnam plain, south & east-south coastal, south mid-mountainous, middle plain area of Korean peninsula.

Chemical Constituents in Polygonum multiflorum Thunberg Root Based on Various Dry Methods (건조방법에 따른 적하수오(Polygonum multiflorum Thunberg)의 이화학적 성분)

  • Oh, Junseok;Hong, Jae-Heoi;Park, Tae-Young;Yun, Kyeong-Won;Kang, Kyeong-Yun;Jin, Seong-Woo;Kim, Kyung-Je;Ban, Seung-Eon;Im, Seung-bin;Koh, Young-Woo;Seo, Kyoung-Sun
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.283-293
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    • 2018
  • This study was performed to analysis of chemical constituent in Polygonum multiflorum root (PMR) by different dry methods (hot-air dry, shade dry, and freeze dry). The results are summarized as followings; major free sugar were detected fructose, glucose, and sucrose in dried PMR based on various dry methods. The highest content of free sugars was found in freeze dried PMR. The four organic acids were detected in dried PMR by HPLC analysis. The content of oxalic acid in shade dried PMR was higher than the dried PMR by different dry methods. The content of total amino acid and essential amino acids were high in the orders of freeze drying > shade drying > hot-air drying. The potassium and magnesium levels of freeze dried PMR was significantly higher than the other drying method of PMR. Whereas the calcium and sodium levels were higher in hot-air dried PMR. The major fatty acids were determined the linoleic acid in PMR by different dry methods.

The Tool to Design Sustainable Business Models: A Case Study for the Social Ventures (지속가능한 비즈니스모델 설계 도구: 소셜벤처 사례를 중심으로)

  • Park, JaeWhan;Jeon, Hyejin
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.1
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    • pp.187-198
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    • 2019
  • The purpose of this study is to seek ways of utilizing TLBMC by understanding business model of social ventures that are accompanied by social and environmental as well as economic missions. In order to achieve this, business models from economic, environmental and social perspectives will be analyzed, and we seek to enhance sustainability of social venture entrepreneurs. As the analysis tool, TLBMC(Triple Layered Business Model Canvas) expands upon the business model canvas that is widely utilized and recognizes economical terms. The TLBMC is proposed by Joyce, A., & Paquin, R. L.(2016) to help achieve a holistic view with horizontal and vertical associations. The study tries to overcome limitations of previous studies and observe a variety of economic, environmental, and social perspectives that social ventures should have with the TLBMC. As a result, it has the following implications; Firstly, creating separate social, environmental and economic business model canvas helps a business to have a holistic approach. Secondly, it was found that social venture characteristics of environmental and social perspectives were applied in practice. Lastly, we have established experience data on social venture business model. This study focuses on the opinions, the meanings and the subjective views of the participants. As a result, conclusions are drawn by the researchers ' assertions and has limitations as a research on case studies. However, this study will help people who are preparing or studying social ventures to have economic, environmental, and social perspectives. Also, redefinition of the direction and value of entrepreneurs operating social ventures, such as vision and mission, will help clarify the roles and responsibility of organizations. As a fundamental step for future empirical studies, this study could be the base for social venture business model studies.