• Korean Journal of Veterinary Research
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• v.52 no.1
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• pp.25-31
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• 2012

Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.

• Journal of applied mathematics & informatics
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• v.34 no.5_6
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• pp.369-382
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• 2016

In this paper, we investigate the global behavior of the solutions of the difference equation $x_{n+1}=\frac{A+Bx_{n-2k-1}}{C+D\prod_{i=l}^{k}x_{n-2i}^{m_i}}$, n=0, 1, ..., with non-negative initial conditions, the parameters A, B are non-negative real numbers, C, D are positive real numbers, k, l are fixed non-negative integers such that l ≤ k, and m_i, i=l, k are positive integers.

• Journal of Life Science
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• v.14 no.4
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• pp.614-619
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• 2004

This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/gㆍfresh weight and 547 unit/mgㆍprotein, respectively, and those in L. japonica were 23 unit/gㆍfresh weight and 13 unit/mgㆍprotein, respectively SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.

• Bulletin of the Korean Mathematical Society
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• v.57 no.3
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• pp.649-660
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• 2020

This paper proves a new regularity criterion for solutions to the Cauchy problem of the 3D Boussinesq equations via one directional derivative of the horizontal component of the velocity field (i.e., (∂_iu₁; ∂_ju₂; 0) where i, j ∈ {1, 2, 3}) in the framework of the anisotropic Lebesgue spaces. More precisely, for 0 < T < ∞, if $$\large{\normalsize\displaystyle\smashmargin{2}{\int\nolimits_o}^T}({\HUGE\left\|{\small{\parallel}{\partial}_iu_1(t){\parallel}_{L^{\alpha}_{x_i}}}\right\|}{\small^{\gamma}_{L^{\beta}_{x_{\hat{i}}x_{\bar{i}}}}+}{\HUGE\left\|{\small{\parallel}{\partial}_iu_2(t){\parallel}_{L^{\alpha}_{x_j}}}\right\|}{\small^{\gamma}_{L^{\beta}_{x_{\hat{i}}x_{\bar{i}}}}})dt<{{\infty}},$$ where ${\frac{2}{{\gamma}}}+{\frac{1}{{\alpha}}}+{\frac{2}{{\beta}}}=m{\in}[1,{\frac{3}{2}})$ and ${\frac{3}{m}}{\leq}{\alpha}{\leq}{\beta}<{\frac{1}{m-1}}$, then the corresponding solution (u, θ) to the 3D Boussinesq equations is regular on [0, T]. Here, (i, ${\hat{i}}$, ${\tilde{i}}$) and (j, ${\hat{j}}$, ${\tilde{j}}$) belong to the permutation group on the set 𝕊₃ := {1, 2, 3}. This result reveals that the horizontal component of the velocity field plays a dominant role in regularity theory of the Boussinesq equations.

• Korean Journal of Materials Research
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• v.2 no.5
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• pp.330-336
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• 1992

Four distinctive hot pressed and heat treated S${i_3}{N_4}$ceramics, S${i_3}{N_4}$-8%${Y_2}{O_3}$, S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$, S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$, 그리고 S${i_3}{N_4}$-1% MgO-1% Si$O_2$(in wt%), were prepared and characterized by X-ray diffraction, scanning electron microscopy, image analysis and mechanical tests. The fracture toughness of S${i_3}{N_4}$-8% ${Y_2}{O_3}$specimens containing large elongated grains showed the highest value of about 9.8MPa$m^{1/2}$. Two out of four S${i_3}{N_4}$, ceramics(S${i_3}{N_4}$-6% ${Y_2}{O_3}$-2% $A{l_2}{O_3}$and S${i_3}{N_4}$-4% ${Y_2}{O_3}$-3% $A{l_2}{O_3}$) heat treated at 200 $0^{\circ}C$retained the fracture strength of over 900MPa and fracture toughness of over 8.0MPa$m^{1/2}$. Large ${\beta}$-S${i_3}{N_4}$grains having a diameter larger than 1${\mu}$m appeared to contribute to increase in fracture toughness.

• Molecules and Cells
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• v.24 no.3
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• pp.351-357
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• 2007

Regions (about 3.7-3.8 kb) of the mitochondrial genomes (rrnL-cox1) of two tardigrades, a heterotardigrade, Batillipes pennaki, and a eutardigrade, Pseudobiotus spinifer, were sequenced and characterized. The gene order in Batillipes was $\underline{rrnL}-\underline{V}-\underline{rrnS}-\underline{Q}-\underline{I}$-M-nad2-W-$\underline{C}-\underline{Y}$-cox1, and in Pseudobiotus it was $\underline{rrnL}-\underline{V}-\underline{rrnS}-\underline{Q}$-M-nad2-W-$\underline{C}-\underline{Y}$-cox1. With the exception of the trnI gene, the two tardigrade regions have the same gene content and order. Their gene orders are strikingly similar to that of the chelicerate Limulus polyphemus (rrnL-V-rrnS-CR-I-Q-M-nad2-W-C-Y-cox1), which is considered to be ancestral for arthropods. Although the tardigrades do not have a distinct control region (CR) within this segment, the trnI gene in Pseudobiotus is located between rrnL-trnL1 and trnL2-nad1, and the trnI gene in Batillipes is located between trnQ and trnM. In addition, the 106-bp region between trnQ and trnM in Batillipes not only contains two plausible trnI genes with opposite orientations, but also exhibits some CR-like characteristics. The mitochondrial gene arrangements of 183 other protostomes were compared. 60 (52.2%) of the 115 arthropods examined have the M-nad2-W-C-Y-cox1 arrangement, and 88 (76.5%) the M-nad2-W arrangement, as found in the tardigrades. In contrast, no such arrangement was seen in the 70 non-arthropod protostomes studied. These are the first non-sequence molecular data that support the close relationship of tardigrades and arthropods.

• Bulletin of the Korean Chemical Society
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• v.26 no.10
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• pp.1529-1532
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• 2005

A simple and cost effective method for separation and preconcentration of Ag(I) at the $10^{-7}\;mol\;L^{-1}$ level in the environmental and mineral samples is present. The method is based on the flotation of Ag(I)-iodide complex as an ion-associate with ferroin in pH of 4 from a large volume of an aqueous solution (500 mL) using nheptane. The floated layer was then dissolved in dimethylsulfoxide (DMSO) for the subsequent spectrophotometric determination. Beer's law was obeyed over a range of 2.0 ${\times}$ $10^{-7}$-4.0 ${\times}$ $10^{-6}$ mol $L^{-1}$ with the apparent molar absorptivity of 2.67 ${\times}$ $10^5$ L $mol^{-1}\;cm^{-1}$. The detection limit (n = 5) was 4 ${\times}$ $10^{-8}$ mol $L^{-1}$, and RSD (n = 5) obtained for 2.0 ${\times}$ $10^{-6}$ mol $L^{-1}$ of Ag(I) was 2.2%. The interference effects of a number of elements was studied and found that only $Hg^{2+}$ at low concentration, and $Pb^{2+}$, $Cd^{2+}$, $Cu^{2+}$, and $Fe^{3+}$ ions at moderately high concentrations were interfered. To overcome on these interference effects, the solution was treated with EDTA at a buffering pH of 4 and passed through a column containing Amberlite IR-120 ionexchanger resin, just before the flotation process. The proposed method was applied to determine of Ag(I) in a synthetic waste water, a photographic washing sample and a geological sample and the results was compared with those obtained from the flame atomic absorption spectrometry. The results were satisfactorily comparable with together, so that the applicability of the proposed method was confirmed in encountering with the real samples.

• Proceedings of the KSPS conference
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• 1996.10a
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• pp.521-528
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• 1996

This presentation explores on the perceptual characteristics of the lateral sound /l/ in CV syllables. At initial position we found that /l/ has well marked formant transitions. Then several questions arise: 1) are these formant structures dependent on the following vowel\ulcorner. 2) Are the formant transitions giving an additional cue for the identification\ulcorner Considering that the French vocalic system presents a greater variety of vowels than Spanish, several experiments were designed to verify to what extent a more extensive range of vocalic timbres contribute to the perception of /l/. Natural emissions of /l/ produced in Argentine Spanish and Canadian French CV syllables were recorded, where V was successively /i, e, a, o, u/ for Spanish and /i, e, $\varepsilon$, a, $\alpha$, o, u, y, \phi$/ for French. For each item, the segment C was maintained and V was replaced by cutting & splicing by each of the remaining vowels without transitions. Results of the identification tests for Spanish show that natural /l/ segments with low Fl and high formants F3, F4 can be clearly identified in the /i, e, u/ vowel contexts without transitions. For French subjects the combination of /l/ with a vowel without transitions reflected correct identifications for its own original vowel context in /e, $\varepsilon$, y, $\phi$/. For both languages, in all these combinations, F1 values remained rather steady along the syllable. In the case of /o, u/ very likely the F2 difference lead to a variety of perceptions of the original /l/. For example in Ilul, French subjects reported some identifications of /l/ as a vowel, mainly /y/. Our observations reinforce the importance of F1 as a relevant cue for /l/, and the incidence of the relative distance between formants frequencies of both components.

• Honam Mathematical Journal
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• v.37 no.1
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• pp.135-147
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• 2015

Owing to the notion of a normal adjacency for a digital product in [8], the study of product properties of digital topological properties has been substantially done. To explain a normal adjacency of a digital product more efficiently, the recent paper [22] proposed an S-compatible adjacency of a digital product. Using an S-compatible adjacency of a digital product, we also study product properties of digital topological properties, which improves the presentations of a normal adjacency of a digital product in [8]. Besides, the paper [16] studied the product property of two digital covering maps in terms of the $L_S$- and the $L_C$-property of a digital product which plays an important role in studying digital covering and digital homotopy theory. Further, by using HS- and HC-properties of digital products, the paper [18] studied multiplicative properties of a digital fundamental group. The present paper compares among several kinds of adjacency relations for digital products and proposes their own merits and further, deals with the problem: consider a Cartesian product of two simple closed $k_i$-curves with $l_i$ elements in $Z^{n_i}$, $i{\in}\{1,2\}$ denoted by $SC^{n_1,l_1}_{k_1}{\times}SC^{n_2,l_2}_{k_2}$. Since a normal adjacency for this product and the $L_C$-property are different from each other, the present paper address the problem: for the digital product does it have both a normal k-adjacency of $Z^{n_1+n_2}$ and another adjacency satisfying the $L_C$-property? This research plays an important role in studying product properties of digital topological properties.

• Journal of Life Science
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• v.21 no.10
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• pp.1415-1421
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• 2011

The purpose of this study was to identify the effects of an L-arginine supplementation and regular exercise training on NF-${\kappa}B$, TNF-${\alpha}$, iNOS, Cav-1, eNOS and Ang II in the aortas of D-galactose (D-gal) induced aging rats. The male Strague-Dawley rats were treated with a D-galactose aging inducing agent; the D-gal injection (50 mg/kg) was given intraperitoneally for 12 wk. Experimental groups were divided into five groups: (1) Young control group (Y-Con, n=8), (2) Aging control group (A-Con, n=8), (3) Aging exercise group (A-Ex, n=8), (4) Aging exercise group with L-arginine supplementation group (A-Ex+A, n=8), and (5) Aging with L-arginine supplementation group (A-A, n=8). The exercise consisted of running on a treadmill for 60 min/day at 20 m/min for 6 day/wk, at 0% gradient for 12 wk. The L-arginine supplementation was given orally at a dose of 150 mg/kg/day for 12 wk. The findings of this study were as follows: 1. NF-${\kappa}B$, TNF-${\alpha}$, iNOS, Cav-1 and Ang II proteins in the aortas of D-gal induced rats were significantly increased, however, L-arginine supplementation and regular exercise resulted in a significant inhibition in the expression of NF-${\kappa}B$, TNF-${\alpha}$, iNOS, Cav-1 and Ang II proteins. 2. eNOS protein in the aortas of D-gal induced rats was significantly decreased, however, L-arginine supplementation and regular exercise resulted in a significant increase in the expression of eNOS proteins. In conclusion, the findings of the present study reveal that L-arginine supplementation alone or regular exercise alone or in combination with L-arginine supplementation for 12 wk increases anti-inflammatory effects by decreasing NF-${\kappa}B$, TNF-${\alpha}$, and iNOS protein expressions within the aortic tissue. In addition, L-arginine supplementation alone or regular exercise alone or in combination with L-arginine supplementation may prevent endothelial function by up-regulation of eNOS protein in the aortas of D-gal induced aging rats.