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Optimization of Fabrication Conditions for Immunosensor Strip to Detect Escherichia coli O157 : H7 (Escherichia coli O157 : H7 탐지용 면역센서스트립 제작 조건 최적화)

  • Park, So-Jung;Kim, Young-Kee
    • KSBB Journal
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    • v.24 no.3
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    • pp.253-258
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    • 2009
  • In this study, the optimization of fabrication conditions was accomplished to make immuno-strip biosensor by the combination of enzyme linked immunosorbent assay (ELISA) and immuno-chromatographic strip techniques for the detection of Escherichia coli O157 : H7. Optimal fabrication conditions of capture antibody concentration, detection antibody concentration, and additive composition of running buffer solution were determined. Optimal concentration was determined as 1.0 mg/mL for both of capture antibody and detection antibody. A composition of 0.5% Tween20 and 3% BSA were selected as optimal additive for buffer solution to prevent non-specific binding.

Gamakamide C and D as Two New Analogues of Bitter-Tasting Cyclic Peptide with Hydantoin Structure from Oyster Crassostrea gigas

  • Jang, Jun Ho;Park, Taesung;Lee, Jong Soo
    • Fisheries and Aquatic Sciences
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    • v.18 no.2
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    • pp.131-135
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    • 2015
  • Two new bitter-tasting cyclic peptides comprising six amino acids, namely gamakamide C and D, were isolated from cultured oysters Crassostrea gigas. Dimethylaminoazobenzene sulfonyl-amino acid analysis detected Val and Leu in gamakamide C and Ile and Leu in gamakamide D. The molecular formula of gamakamide C was determined as $C_{43}H_{60}N_{7}O_8S$ by high-resolution fast atom bombardment mass spectroscopy (HR FAB-MS) ($[M+H]^+m/z822.4200{\Delta}-2.4mmu$), and that of gamakamide D was determined as $C_{43}H_{62}N_7O_8S$ by HR FAB-MS ($[M+H]^+m/z836.4379{\Delta}-2.0mmu$). Comparison of amino acid analyses and fragment ions by MS/MS among gamakamide C, D, and E (known), the structures of gamakamide C and D were confirmed $as-{\small{L}}-Val-{\small{L}}-Met(SO)-{\small{L}}-NMe-Phe-{\small{L}}-Leu-{\small{D}}-Lys-{\small{L}}-Phe-$ and $-{\small{L}}-Ile-{\small{L}}-Met(SO)-{\small{L}}-NMe-Phe-{\small{L}}-Leu-{\small{D}}-Lys-{\small{L}}-Phe-$, respectively.

ON δ-FRAMES

  • Lee, Seung On;Lee, Seok Jong;Choi, Eun Ai
    • Journal of the Chungcheong Mathematical Society
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    • v.10 no.1
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    • pp.43-56
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    • 1997
  • In this paper, we introduce a new class of ${\delta}$-frames and study its properties. To do so, we introduce ${\delta}$-filters, almost Lindel$\ddot{o}$f frames and Lindel$\ddot{o}$f frames. First, we show that a complete chain or a complete Boolean algebra is a ${\delta}$-frame. Next, we show that a ${\delta}$-frame L is almost Lindel$\ddot{o}$f iff for any ${\delta}$-filter F in L, ${\vee}\{x^*\;:\;x{\in}F\}{\neq}e$. Last, we show that every regular Lindelof ${\delta}$-frame is normal and a Lindel$\ddot{o}$f ${\delta}$-frame is preserved under a ${\delta}$-isomorphism which is dense and codense.

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The Comparison of Photocatalysis and Sonophotocatalysis for Benomyl Degradation (광촉매공정과 초음파를 접목시킨 광촉매공정에 의한 Benomyl의 분해 비교)

  • An, Sang-Woo;Park, Jae-Hong;Cho, Il-Hyoung;Chang, Soon-Woong
    • Journal of Korean Society on Water Environment
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    • v.22 no.4
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    • pp.585-589
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    • 2006
  • Comparison between photocatalysis and sonophotocatalysis were performed in lab-scale experiments for the treatment of benomyl. The effect of operational parameters, i.e., initial benomyl concentration, $TiO_2$ concentration, $H_2O_2$ concentration on the degradation rate of aqueous solution of benomyl has been examined. The optimal conditions for photocatalysis and sonophotocatalysis processes were determined: initial Benomyl concentration was 3 mg/L, the concentration of $TiO_2$ was 2 g/L and $H_2O_2$concentration was 1.5 mM. Under the optimal conditions, sonophotocatalysis was effective for inducing faster degradation of the benomyl.

Comparative Sonophotocatalysis and Photocatalysis for Chloroform Degradation (Sonophotocatalysis와 Photocatalysis를 이용한 Chloroform의 광산화)

  • Park, Jae-Hong;Cho, Il-Hyoung
    • Journal of Korean Society on Water Environment
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    • v.21 no.3
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    • pp.263-266
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    • 2005
  • Comparison between photocatalysis (UV+$TiO_2$) and sonophotocatalysis (Sonication+UV+$TiO_2$) were performed in lab-scale experiments for the treatment of chloroform. The effect of operational parameters, i.e., initial chloroform concentration, $TiO_2$ concentration, UV light intensity and sonication time on the degradation rate of aqueous solution of chloroform has been examined. The optimal conditions for photocatalysis and sonophotocatalysis processes were determined: initial chloroform concentration was 25 mg/L, the concentration of $TiO_2$ was 200 mg/L and UV light intensity was $6.630 mW/cm^2$, respectively. The optimal sonication time on sonophotocatalysis process was 90 min. Under the optimal conditions, sonophotocatalysis was effective for inducing faster degradation of the chloroform.

In silico discovery and evaluation of phytochemicals binding mechanism against human catechol-O-methyltransferase as a putative bioenhancer of L-DOPA therapy in Parkinson disease

  • Rath, Surya Narayan;Jena, Lingaraja;Bhuyan, Rajabrata;Mahanandia, Nimai Charan;Patri, Manorama
    • Genomics & Informatics
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    • v.19 no.1
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    • pp.7.1-7.13
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    • 2021
  • Levodopa (L-DOPA) therapy is normally practised to treat motor pattern associated with Parkinson disease (PD). Additionally, several inhibitory drugs such as Entacapone and Opicapone are also cosupplemented to protect peripheral inactivation of exogenous L-DOPA (~80%) that occurs due to metabolic activity of the enzyme catechol-O-methyltransferase (COMT). Although, both Entacapone and Opicapone have U.S. Food and Drug Administration approval but regular use of these drugs is associated with high risk of side effects. Thus, authors have focused on in silico discovery of phytochemicals and evaluation of their effectiveness against human soluble COMT using virtual screening, molecular docking, drug-like property prediction, generation of pharmacophoric property, and molecular dynamics simulation. Overall, study proposed, nine phytochemicals (withaphysalin D, withaphysalin N, withaferin A, withacnistin, withaphysalin C, withaphysalin O, withanolide B, withasomnine, and withaphysalin F) of plant Withania somnifera have strong binding efficiency against human COMT in comparison to both of the drugs i.e., Opicapone and Entacapone, thus may be used as putative bioenhancer in L-DOPA therapy. The present study needs further experimental validation to be used as an adjuvant in PD treatment.

Pharmacognostical Studies on the Chinese Crude Drug ‘Maig Moon Dong’ (한약 "맥문동" 의 생약학적 연구)

  • Geon, Dai-Gun;Park, Jong-Hee
    • Korean Journal of Pharmacognosy
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    • v.34 no.1 s.132
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    • pp.6-9
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    • 2003
  • ‘Maig Moon Dong(麥問冬)’ is one of the chinese crude drugs used mainly to cure a cough and sputum, etc. With regard to the botanical origin of ‘Maig Moon Dong’, it has been considered to be Liriope species of Liliaceae, but there has not been studied pharmacognostically. To clarify the botanical origin of Maig Moon Dong, we studied on the anatomical characteristics of Liriope and Ophiopogon species growing wild in Korea i.e. L. platyphylla, L. spicata, O. jaburan, O. japonicus and Maig Moon Dong from Korea. As a result, the botanical origin of Maig Moon Dong from Korea was proved as Liriope platyphylla and L. spicata.

A study on the Corrosion behavior of SUS316L (SUS316L의 산화 및 Ar-1%SO2 부식 거동 연구)

  • Kim, Min-Jeong;Park, Sang-Hwan;Ji, Gwon-Yong;Jeong, Seok-U;Hwang, Sang-Yeon;Lee, Dong-Bok
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2015.05a
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    • pp.119-120
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    • 2015
  • SUS316L의 내부식성을 증진시키기 위해 우수한 내부식성 합금원소로 알려진 Al, Cr을 SUS316L에 Sputtering, E-beam evaporation하여 Al, Cr 코팅층을 형성시킨 후 대기 중, $Ar-1%SO_2$ 분위기에서 부식시켰다. 그 결과 코팅층에 의해 $600^{\circ}C$까지 $Al_2O_3$, $Cr_2O_3$ 형성되어 보호피막 역할을 함으로써 내부식성이 증진되었다.

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Il riassunto Aspetto di ricomparsa e maniera d'essere di gli altri e il soggetto -sulla letteratura italiana della migrazione- (주체와 타자의 존재방식과 재현 양상 -이탈리아 이주문학을 중심으로-)

  • Kim, Hee-Jeong
    • Lettere Italiane
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    • no.32
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    • pp.23-48
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    • 2011
  • Se siamo d'accordo la definizione di Deleuze e Guattari che la letteratura minore è la letteratura che costruisce una minoranza dentro la lingua maggiore, le scritture italofoniche sono la traduzione in pratica di un concetto della letteratura minore che fornisce modelli sostitutivi di espressione. Questa minoranza di bilingue, o più precisamente, multilingue autori e pratiche, acquisisce uno spazio dentro della cultura e la lingua italiana, che di conseguenza sono entrambi deterritorializzati. Lo stanziamento di italiano come lingua in cui sono scritti i testi italofonici richiede una pratica influenza linguistica e culturale. Questa ibridazione linguistica e letteraria riverbera la pluralità sociale presente nella moderna Italia, dove oltre un milione di immigrati provenienti da tutto il mondo vivono oggi. Questo collegamento tra il letterario e il sociale si diffonde il senso di deterritorializzazione linguistica per definire personificazioni di 'otherness', sia per i corpi degli immigrati stessi sia per la politica. I testi italofonici rapresentano contemporaneamente l'individuo e il collettivo, cioè, l'esperienza individuale e la vita delle comunità di immigrati.

Spetroscopic Diagnostics of Reactive Plasma in a Facing Target Sputtering Unit (대향타겟트 스파터기에서 반응성 플라즈마의 스펙트로스코프 검진)

  • Na, Jong-Gab;Lee, Taek-Dong;Park, Soon-Ja
    • Korean Journal of Materials Research
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    • v.2 no.5
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    • pp.337-342
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    • 1992
  • Spectroscopic diagnostics on reactive plasmas was carried out in a facing target sputtering unit with BaO +12Fe composite targets and 50% $O_2+$ Ar sputter gas. Spectra of rective plasmas were composed of peaks which were assigned to be Ba, B$a^+$, Fe, FeO, F$e^+$, Ar, $Ar^+$, O, $O^+$. As detecting positions in plasmas were far away from targets, the relative peak intensities of the ions and neutral species were decreased, but the relative intensities of the former decreased faster than those of the latter.

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