• Plant Biotechnology Reports
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• v.5 no.1
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• pp.61-69
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• 2011

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.89-90
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• 1999

페레니얼 라이그라스(Lolium perenne L.)의 질소공급형태에 따른 저장질소의 함량과 재생기간 중 저장질소의 이용 및 재생활력을 구명하고자 수경재배 하였다. (NH$_4$)$_2$SO$_4$, KNO$_3$, Urea, Organic-N 및 NH$_4$NO$_3$의 서로 다른 질소형태로 각각 1mM/week을 4주간 공급하여 건물생산량을 보았고, 예취 후 각각의 질소 형태로 4mM/40days동안 공급하여 흡수된 질소의 함량 및 잔여기관내 축적된 질소의 함량을 관찰하였으며, 예취 후 저장된 질소에 의한 재생활력을 보기 위해 0.2mM NH$_4$NO$_3$의 형태로 동일하게 공급하였다.(중략)

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.12
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• pp.1722-1727
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• 2006

The nutritive value of 4 straws, obtained after thrashing of seeds from fodder crops, was assessed as complete feed for ruminants. Sixteen male Murrah buffaloes (liveweight 365.8${\pm}$19.5 kg), were divided into 4 equal groups and offered ad lib. straw of either Trifolium resupinatum, Trifolium alexandrium, Medicago sativa or Lolium perenne, supplemented with minerals and vitamin A, for 40 days in a completely randomized design. Simultaneously, each straw was offered to 3 rumen fistulated male buffaloes in order to assess the biochemical changes in the rumen. Compared to other straws M. sativa straw had higher (p<0.05) organic matter (OM), crude protein (CP), acid-detergent fiber (ADF) and cellulose content. L .perenne had the highest (p<0.05) hemicellulose and lowest (p<0.05) CP and acid-detergent lignin (ADL) content. T. resupinatum had the lowest concentration of cell wall constituents (CWC). The digestibility of nutrients of T. resupinatum and L. perenne straw was similar, but higher (p<0.05) than that of other straws. M.sativa straw showed highest (p<0.05) digestibility of CP. The highest OM digestibility of T. resupinatum and CP digestibility of M. sativa were responsible for highest (p<0.05) total volatile fatty acids and trichloroacetic acid precipitable nitrogen in the strained rumen liquor. The digestible crude protein (DCP) was highest (p<0.05) in M. sativa followed by that in T. alexandrium. The total purine derivatives excreted in urine varied from 0.22-0.32 mmol/kg $W^{.75}/d$. The efficiency of microbial protein synthesis indicated that OM of straws of M. sativa and that of T. alexandrium was used more (p<0.05) efficiently. The microbial protein synthesized was highest in T. resupinatum, but statistically similar to other groups. The values for N-retention and apparent biological value were highest for L. perenne, though comparable with that of M. sativa and T. alexandrium. The available metabolizable energy (ME) was highest (p<0.05) in T. resupinatum followed by that in L. perenne and lowest in M. sativa. It was concluded that all the straws, supplemented with minerals and vitamin A, could be fed exclusively to adult ruminants with no adverse affect, as animals were able to maintain body weight (372${\pm}$20.1 kg).

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.235-240
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• 2007

Optimum tissue culture conditions for an efficient induction of embryogenic callus from mature seeds of perennial ryegrass (Lolium perenne L.) and regeneration of plants from callus tissues were investigated. MS medium containing 3 mg/L 2,4-D and 0.1 mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (58.3%) was observed when the embryogenic callus tissues were cultured on N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be helpful for molecular breeding of perennial ryegrass through Agrobacterium-mediated genetic transformation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.19-24
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• 2000

This study was camed out in order to establish plant regeneration via seed-derived callus of perennialryegrass.Varietal difference in callus growth and plant regeneration was obvious between two cultivars of perennialryegrass. "Reveille" showed a relatively high capacity for plant regeneration. The MS medium was superiorto SH or Bj in callus formation and plant regeneration. The highest regeneration frequency (60%) from calluswas obtained in presence of 5 mg/ l 2,4-D and 1 mg/ t kinetin. Regeneration response varied among calluscultures initiated from the same cultivar. Regeneration frequency was the most effective in 6 weeks-old calliafter initiation and lost their regeneration capacity gradually over a period of 12 weeks.(Key words : Perennial ryegrass (Lolium perenne L.), Plant regeneration)e L.), Plant regeneration)

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.15-20
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• 2001

Plant growth regulators were treated on the cut perennial ryegrass (Lolium perenne L. cv. Reveille) to investigate the effect on the regrowth after cutting. The growth showed better result when 0.1 or 0.5 mg/L cytokinin were treated. Among cytokinins, kinetin or 2iP gave the better effect on the growth than BAP. In 2.4-D as an auxin. cut plants grew best at the concentration of 0.1 mg/L. The initial regrowth was very vigorous when GA$_3$ was treated as a growth regulator, but the growth was retarded after 2 weeks later of cutting. Co-treatment of kinetin as a cytokinin and 2,4-D as an auxin showed synergistic effect on the regrowth of cut perennial ryegrass. Both plant growth regulators gave the same result at the same concentrations in the suspension culture of perennial ryegrass cells.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.83.1-83
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• 2001

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.97-97
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• 2001

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.2
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• pp.89-96
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• 2001

Carbohydrate and soluble protein reserves and regrowth characteristics in response to cutting height were investigated over four regrowth cycles of turf-type perennial ryegrass(Lolium perenne L. cv. preludeII). When the plants were at the full-vegetative stage (twelve weeks-old), three sequential defoliations at 3, 6 and 9 cm above the root base were imposed at 2-week intervals. Shoot dry weight in all three treatments continuously decreased with progressing regrowth cycle and the decreasing rate was higher as cutting height was lowered. TNC (total non-structural carbohydrate) in stubble at the end of the fourth regrowth cycle in 3, 6 and 9 cm cutting height decreased by 98%, 82% and 27%, respectively, comparing the initial content. TNC in roots also largely decreased with similar pattern in response to cutting height, whereas the absolute amount was much less compared to stubble. Soluble protein in stubble in 3, 6 and 9 cm cutting height decreased by 98%, 82% and 57%, respectively, at the end of fourth regrowth. A significant correlations between TNC (r=0.906) or protein (r=0.879) at the fourth defoliation and dry weight of regrowing shoots at the end of fourth regrowth were observed. these results indicated that cutting height closely influences the levels of organic reserves available for new growth, and that the levels of reserves might provide a useful tool as a determinant for regrowth dynamics.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.88-88
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• 2001