• Title/Summary/Keyword: L. bicolor

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Biological Activity of Sorghum bicolor M. cv. Bulgeunjangmoksusu Extracts (붉은장목수수 추출물의 생리활성)

  • Kim, Joo-Seok;Lee, Yea-Ji;Yang, Jinfeng;Sa, Yeo-Jin;Kim, Myeong-Ok;Park, Jong-Hyuk;Park, Dong-Sik;Yu, Chang-Yeon;Kim, Myong-Jo
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.111-118
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    • 2013
  • The objective of this study was to determine the biological activities of Sorglum bicolor extracts. Organic fractions, including n-Hexane, EtOAc, and n-BuOH fractions were obtained from the methanol extract of Sorglum bicolor M.. In DPPH radical scavenging activity, $SC_{50}$ values of methanol extract and EtOAc fraction were exhibited $0.66{\pm}0.26{\mu}g/mL$ and $1.03{\pm}0.02{\mu}g/mL$, respectively. Contents of total polyphenol and flavonoids in EtOAc fraction, which were much higher than those of other fractions, were 58.12 mg/g and 4.79 mg/g respectively. Also, effects of reducing power was strongly showed in EtOAc fraction. ${\alpha}$-Glucosidase and ${\alpha}$-amlyase inhibition activities were showed the higher effect in D.W. fraction ($2.83{\mu}g/mL$, $36.64{\mu}g/mL$). In MTT assay in the AGS, HT29 and HCT116 cell lines were significantly higher in the n-BuOH fraction than in the other fractions at $50{\mu}g/mL$ concentration of extracts.

Effect of Nitrogen Fertilization and Cutting Management on the Carbohydrate Reserves, Regrowth, and Dry Matter Yeild of Sorghum-Sudangrass Hybrid[Sorghum bicolor(L.)Moench] II. Effect of nitrogen fertilization and cutting height on the dead stubble and carbohydrate reserves in stubble of sorghum-sudangrass hybrid (질소시비수준과 예취관리가 수단그라스계 잡종 [Sarghum Bicolor (L.) Moench]의 저장탄수화물함량, 재생 및 수량에 미치는 영향. II. 질소시비수준과 예취높이가 수단그라스계 잡종의 그루터기 고사와 저장탄수화물함량에 미치는 영향)

  • Seo, Sung;Kim, Dong-Am
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.3 no.2
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    • pp.67-76
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    • 1983
  • This experiment was carried out to investigate the effects of the different nitrogen rates and cutting heights on the dead stubble after cutting, and the carbohydrate reserved in stubble of sorghum-sudangrass hybrid (Sorghum bicolor (L.) Moench) Pioneer 988. The experiment was undertaken in the Experimental Livestock Farm of Agriculture Coll., Seoul Nat'l Univ. In Suweon, 1981 and 1982. The results obtained are summarized as follows: 1. The dead stubble after cutting was found to be great with high rate of nitrogen fertilizer and low cutting height. It was also learned that the rainfall during forage cutting period made the dead stubble increased. 2. A significant higher (p<0.05) carbohydrate reserves in the stubble was observed in the high stubble height at the first cutting time and 6th through 7th day after the first cutting. The results indicate that the high stubble height reserves more carbohydrate for the early regrwoth stage after the first cutting when comparing with the low stubble. 3. The content of carbohydrate reserves was influenced by climete. Drought is caused to high content of carbohydrate, whereas, rain is caused to low content of that. The critical soluble carbohydrate content causing the death of stubble supposed to be 3 to 6% at least. 4. It is suggest that carbohydrate reserves in plant do not play a distinctive role for the regrowth in a summer annual forage like sorghum-sudangrass hybrid, but it might be rather influenced by the other factors, for example, environmental conditions at harvest and new bud.

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Effect of salt stress on the anthocyanin content and associated genes in Sorghum bicolor L.

  • Jeon, Donghyun;Lee, Solji;Choi, Sehyun;Seo, Sumin;Kim, Changsoo
    • Korean Journal of Agricultural Science
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    • v.47 no.1
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    • pp.105-117
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    • 2020
  • Abiotic stress is one of the most serious problems in plant productivity because it dramatically delays plant growth and development. One of the abiotic stresses, soil salinity, has an adverse effect on plant growth, particularly in areas where irrigation is necessary like semiarid Asia and Africa. Among several physiological parameters, anthocyanin accumulation is a valuable indicator of the condition of the plant, and it tends to increase under salt stress conditions because of its protective role in such an environment. Consequently, it may be important to search for well adapted genotypes for upcoming climate changes. Anthocyanins are known to have important roles in defense against biotic and abiotic stresses, providing important functions for protecting plant cells from reactive oxygen species. In this study, we investigated the anthocyanin accumulation between two Korean sorghum genotypes, Sodamchal and Nampungchal. The two genotypes were subjected to a regulated salinity condition, and the anthocyanin contents were evaluated in both. In Nampungchal, the anthocyanin content increased with 150 mM NaCl treatment during the time course of the experiment. However, the anthocyanin content of Sodamchal decreased in the same condition. The measured values of the anthocyanin content should be useful to identify the intensity of the salt tolerance in Sorghum bicolor L. Furthermore, we studied gene expression profiling of salt stress related genes with qRT-PCR. These results suggest that Nampungchal is a more tolerant genotype to salt stress compared to Sodamchal. This information should be useful for breeding salt-resistant cultivars in sorghum.

Comparison of Cytoplasmic Inclusions Induced by Maize Dwarf Mosaic Virus Strain A and B (Maize Dwarf Mosaic Virus strain A와 B에 의해 유도된 세포질 봉입체의 비교분석)

  • Choi, Chang-Won;Gardner, Wayne S.
    • Applied Microscopy
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    • v.24 no.2
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    • pp.105-114
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    • 1994
  • Comparative ultrastructural studies of sorghum (Sorghum bicolor L. Moench) cultivar (cv.) HOK and cv Pioneer 8680 leaf cells separately infected with maize dwarf mosaic virus (MDMV) strain A and B, respectively, revealed the formation of numerous cylindrical inclusions in both cross and longitudinal sections. The MDMV-A and -B were distinguished by the presence or absence of specific inclusion bodies in the cytoplasm. Electron microscope revealed the presence of pinwheels, bundles, scrolls, and laminated aggregates in Pioneer 8680 sorghum leaf cells infected with MDMV-B while no laminated aggregates were found in cells of HOK sorghum leaf cells infected with MDMV-A. Differences in the ultrastructure of cylindrical inclusions between two strains of MDMV, especially with respect to laminated aggregates, have been morphologically indexed to classify potyviruses into subdivision. The presence of laminated aggregates may be assigned to subdivision III while the absence of laminated aggregates is assigned to subdivision I. These variations of structures associated with cylindrical inclusions appeared virus-specific inductions and may be represented the morphogenesis of inclusion bodies following development of infections.

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Reproductive Capacity of the Genus Lespedeza(1) (싸리류(類)의 재생능력(再生能力) (제(第)1보(報)))

  • Han, Young-Chang
    • Journal of Korean Society of Forest Science
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    • v.21 no.1
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    • pp.35-38
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    • 1974
  • In order to investigate reproductive capacity of genus Lespedeza 5 spp,; L. bicolor, L. maximowiczii, L. japonica var. intermedia, L. angustifolioides, and L, cyrtobotrya, their hights, diameter at the ground level, and the number of sprouts were compared with each other at the end of October during investigating years from 1970 to 1972. For measuring of invested items, 6 stocks which the one-year aged stem were cut above ground were randomly planted per square meter with six replications at the spring of 1970. After hights, diameter, and the number of sprouts were measured at the end of October every year, the stem were cut above ground at the end of November from 1970 to 1972. The results are as follows: 1. The reproductive of the stems showed statistically significant differences at 1% level by species and stock ages. L. bicolor showed the best reproductive capacity of them. 2. The sprouting ability was revealed that L. maximowiczii showed the best of them. 3. The reproductive capacity of stem, diameter, and number of sprouts increased with aging of stock up to 4 years on the investigated 5 species.

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Reproductive Capacity of the Genus Lespedeza (II) (싸리류(類)의 재생(再生) 능력(能力) (II))

  • Han, Young Chang
    • Journal of Korean Society of Forest Science
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    • v.35 no.1
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    • pp.15-18
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    • 1977
  • In order to investigate reproductive capacity of genus Lespedeza 5 spp.; L. bicolor, L. maximowiczii, L. japonica var. intermedia, L. angustifolioides, and L. cyrtobotrya, their hights, diameter at the ground level, and the number of sprouts were compared with each other at the end of October during investigating years from 1970 to 1976. For measuring of invested items, 6 stocks which the one-year aged stem were cut above ground were randomly planted per square meter with six replications at the spring of 1970. After hights, diameter, and the number of sprouts were measured at the end of October every year, the stem were cut above ground at the end of November from 1970 to 1976. The results are as follows: 1. The reproductive of the stems showed statistiaclly significant differences at 1% level by species and stock ages. L. bicolor showed the best reproductive capacity of them. 2. The sprouting ability was revealed that L. maximowiczii showed the best of them. 3. The reproductive capacity of stem, diameter, and number of sprouts increased with aging of stock up to 5 years on the investigated 5 species.

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Agronomic Characterization of Sorghum bicolor (L) Moench Germplasm Collected in Gangwon-do for Bio-energy Crop (바이오에너지 자원으로서 강원도 지역에서 수집된 수수의 작물학적 특성 평가)

  • Seong, Eun-Soo;Yoo, Ji-Hye;Lee, Jae-Geun;Hwang, In-Seong;Kim, Nam-Jun;Kim, Myong-Jo;Lee, Ju-Kyung;Ghimire, Bimal Kumar;Lim, Jung-Dae;Yu, Chang-Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.151-158
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    • 2011
  • Forty nine accessions of the Sorghum bicolor (L) Moench collected from different agronomical zone of Gangwondo province, Korea were characterized morphologically on field trial. Plant length, stem diameter, tiller number, ear type, ear length, ear width, leaves length, leaves width and leaf color differed significantly in the cultivated accessions. Out of forty nine test accessions, six accessions (12.2%) showed comparatively taller height, measuring more than 300 cm, while, other ten test accessions (20.4%) were measured from 200~300 cm. There were no significant differences in the diameter of stem in the collected test accessions (2~3 cm). Of the total test accession, seven accessions showed leaf length of over 70 cm, and twenty two test accession revealed leaf length between 60-70 cm. Forty nine of the collected test accessions showed narrow and short leaves with white mid vain. These results provided additional information for the breeding programs and conservation of Sorghum bicolor as a bio-energy crop.

Sorghum Extract Enhances Caspase-dependent Apoptosis in Primary Prostate Cancer Cells and Immune Activity in Macrophages (수수 추출물에 의한 primary 전립선 암세포의 caspase 의존성 apoptosis 유도 및 대식세포 면역활성 증가)

  • Cho, Hyun-Dong;Kim, Jeong-Ho;Hong, Seong-Min;Lee, Ju-Hye;Lee, Yong-Seok;Kim, Du-Hyun;Seo, Kwon-Il
    • Journal of Life Science
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    • v.26 no.12
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    • pp.1431-1437
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    • 2016
  • Sorghum bicolor L. is one of the important minor cereals in Asia, Africa, and the central United States, and it is considered a rich source of polyphenols, flavonoids, and dietary fiber. However, there is a lack of data on the anti-cancer activity of Sorghum in prostate cancer cells and immune activity in macrophages. This study aims to investigate the potential effects of an ethanol extract of S. bicolor L. (SE) on inducing apoptosis in RC-58T/h/SA#4 cells and immunomodulatory activity in RAW 264.7 cells. SE significantly inhibited the viability of RC-58T/h/SA#4 primary prostate cancer cells in a dose-dependent manner. The morphology of RC-58T/h/SA#4 cells treated with SE was shrunken and involved the formation of an apoptotic body and nuclear condensation. In addition, SE markedly activated caspase-8, -9, and -3; increased the protein levels of Bax, p53, cleaved PARP, and cytosolic cytochrome c; and decreased Bcl-2 protein expression. Furthermore, the inhibition of caspases in RC-58T/h/SA#4 cells with z-VAD-fmk attenuated SE-induced cell growth inhibition. The production of nitric oxide (NO) was also elevated by SE treatment, as revealed by immune response parameters. These results suggest that SE inhibits growth and induces apoptosis in primary human prostate cancer cells in a caspase-dependent manner, and it modulates the immune functions in macrophages. Therefore, Sorghum bicolor L. may be used as a functional food to prevent prostate cancer and enhance immune activity.