• Archives of Pharmacal Research
• /
• 제16권4호
• /
• pp.312-317
• /
• 1993

To confirm a new evaluation tedhnique for biodegradability of biopolymer microsphers in vivo condition, magnetic microsphere sytem was adopted for tracing the microspheres injected and lodged in micr. Microsphers of poly(DL-lactic acid), poly(L-alctic acid) and poly(DL-lactide-coglycolide)(PLGA) were prepared by solvent-extraction method and their organ distribution and biodegradation in mice was examined. Magnetic microspheres lodged in mice organs were recollected from the homogenates of mice organs with a constant flow magnetic separation apparatus. Recollected microspheres were observed by scanning electron microscopy and also were assayed for their magnetite ocntent by atomic absorption spectrophotometry to evaluate the biodegradability of polymeric microspheres. This method seems to be practical and simple to estimate the biodegradability of biopolymers over the conventional methods.

• Journal of Microbiology and Biotechnology
• /
• 제11권4호
• /
• pp.607-613
• /
• 2001

The biodegradation rates of diesel oil by a selected diesel-degrading bacterium, Pseudomonas stutzeri strain Y2G1, and microbial consortia composed of combinations of 5 selected diesel-degrading bacterial were determined in liquid and soil systems. The diesel degradation rate by strain Y2G1 linearly increased $(R^2=0.98)$ as the diesel concentration increased up to 12%, and a degradation rate as high as 5.64 g/l/day was obtained. The diesel degradation by strain Y2G1 was significantly affected by several environmental factors, and the optimal conditions for pH, temperature, and moisture content were at pH8, $25^{\circ}C$, and 10%, respectively. In the batch soil microcosm tests, inoculation, especially in the form of a consortium, and the addition of nutrients both significantly enhanced the diesel degradation by a factor of 1.5 and 4, respectively. Aeration of the soil columns effectively accelerated the diesel degradation, and the initial degradation rate was obviously stimulated with the addition of inorganic nutrients. Based on these results, it was concluded that the major rate-limiting factors in the tested diesel-contaminated soil were the presence of inorganic nutrients, oxygen, and diesel-degrading microorganisms. To resolve these limiting parameters, bioremediation strategies were specifically designed for the tested soil, and the successful mitigation of the limiting parameters resulted in an enhancement of the bioremediation efficiency by a factor of 11.

• Journal of Microbiology and Biotechnology
• /
• 제26권10호
• /
• pp.1765-1773
• /
• 2016

Wastewater containing kraft lignin (KL) discharged from pulp and paper industries could cause serious environmental contamination. Appropriate effluent treatment is required to reduce the pollution. Investigations on anaerobic bacteria capable of degrading KL are beneficial to both lignin removal and biofuel regeneration from the effluent. In this paper, an anaerobic strain capable of degrading KL was isolated from the sludge of a pulp and paper mill and identified as Dysgonomonas sp. WJDL-Y1 by 16S rRNA analysis. Optimum conditions for KL degradation by strain WJDL-Y1 were obtained at initial pH of 6.8, C:N ratio of 6 and temperature of 33℃, based on statistical analyses by response surface methodology. For a 1.2 g/l KL solution, a COD removal rate of 20.7% concomitant with biomass increase of 17.6% was achieved after 4 days of incubation under the optimum conditions. After the treatment by strain WJDL-Y1, KL was modified and degraded.

• Journal of Microbiology and Biotechnology
• /
• 제15권5호
• /
• pp.946-951
• /
• 2005

Biodegradation of the endocrine-disrupting chemical di-n-butyl phthalate (DBP) was investigated using a bacterium, Pseudomonas fluorescens B-1, isolated from mangrove sediment. The effects of temperature, pH, salinity, and oxygen availability on DBP degradation were studied. Degradation of DBP was monitored by solid-phase extraction using reversed-phase HPLC and UV detection. The major metabolites of DBP degradation were identified as mono-n-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry (GC-MS) and a pathway of degradation was proposed. Degradation by P. fluorescens B-1 conformed to first-order kinetics. Degradation of DBP was also tested in seawater by inoculating P. fluorescens B-1, and complete degradation of an initial concentration of $100{\mu}g/l$ was achieved in 144 h. These results suggest that DBP is readily degraded by bacteria in natural environments.

• Environmental Engineering Research
• /
• 제13권1호
• /
• pp.8-13
• /
• 2008

The extent of solubility enhancement by biosurfactant was examined at various pHs prior to the biodegradation experiments. The molar solubilization ratio (MSR) was calculated from the batch solubilization experiments and the highest MSR was detected at pH 5. The effect of the biosurfactant, rhamnolipids, on the phenanthrene mineralization in soil-water system was investigated. The strain 3Y was selected for the mineralization assay and large amounts of phenanthrene were degraded at neutral pH in soil-water system without the biosurfactant. The addition of 150 mg/L rhamnolipids showed no effect on mineralization of phenanthrene in soil-water system, and total mineralization rates after 6 weeks incubation at each pH showed no differences in presence and absence of rhamnolipids. Our result indicated that the toxic effect of rhamnolipids can disappear when soil particles exist, and also the enhanced solubility of phenanthrene does not work for mineralization enhancement in this soil-water system.

• KSBB Journal
• /
• 제14권3호
• /
• pp.315-321
• /
• 1999

The relationships between the explosive 2,4,6-trinitrotoluene (TNT) degradation by Stenotrophomonas maltophilia and several relevant physicochemical environmental parameters were examined. At neutral pH of the cultures, the degradation of TNT proceeded to completion, whereas only about 50% of TNT was utilized when the cultures were adjusted to acidic pH. The effect of various co-substrates (e.g., glucose, fructose, acetate, citrate, succinate) on the degradation of TNT by the test culture of S. maltophilia was evaluated. The results indicated that, among the various co-substrates studies, the test culture that received 2 mM fructose degraded 100 mg/L of TNT completely within 20 days of incubation at ambient temperature, whereas partial degradation of TNT was observed in the test culture with acetate, citrate, or succinate as a co-substrate, respectively. In fact, fructose was the best co-substrate for TNT degradation in this experiment. The effect of supplemented nitrogens [e.g., (NH$_4$)$_2$,SO$_4$, NH$_4$Cl. urea] on the TNT degradation was monitored. All supplemented nitrogens in this study were inhibitory to TNT degradation. Addition of 1% Tween80 accelerated TNT degradation, and showed complete degradation of TNT within 8 days of incubation. Addition of yeast extract resulted higher growth yields, based on turbidity measurement, but it inhibited TNT degradation.

• 한국환경농학회지
• /
• 제30권2호
• /
• pp.202-208
• /
• 2011

BACKGROUD: ${\alpha}$- and ${\beta}$- Endosulfan isomers of endosulfan, an endocrine disrupting chemical, are widely used cyclodiene organochlorine pesticide in worldwide, and it has widespread application in agriculture and can contaminate river-system as runoff from soil or aerial deposition METHOD AND RESULTS: In this study, an attempt was made to isolate an endosulfan degrading fungus from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture in media containing endosulfan and its metabolites as the sole carbon source, a fungus designated KEF-1 was isolated. Based on phylogenetic analysis, strain KEF-1 was assigned to the genus Eutypella. Also, the ITS (internal transcribed spacer) sequences of KEF-1 were submitted to GenBank under accession number EF581006. In potato dextrose broth containing 8 ${\mu}g$/mL endosulfan, strain KEF-1 completely degraded the endosulfanin 12 days. CONCLUSION: These results suggested that Eutypella sp. KEF-1 has potential as a biocatalyst for endosulfan bioremediation

• Journal of Industrial and Engineering Chemistry
• /
• 제67권
• /
• pp.219-230
• /
• 2018

The present study examined a moving bed biofilm reactor (MBBR) bioreactor on a laboratory scale for simultaneous removal of atrazine, organic carbon, and nutrients from wastewater. The maximum removal efficiency of atrazine, chemical oxygen demand (COD), total phosphorus (TP) and total nitrogen (TN) were 83.57%, 90.36%, 90.74% and 87.93 respectively. Increasing salinity up to 40 g/L NaCl in influent flow could inhibit atrazine biodegradation process strongly in the MBBR reactor.Results showed that MBBR is so suitable process for efficiently biodegrading of atrazine and nitrogen removal process was based on the simultaneous nitrification-denitrification (SND) process.

• 청정기술
• /
• 제28권1호
• /
• pp.32-37
• /
• 2022

Food waste is produced from food factories, food services, and home kitchens. The generated mass reached 5.4 million tons/year in 2020. The basic management technology for such waste has been biological degradation under an anaerobic environment. However, the whole process is intrinsically slow and considerably affected by the inner physicochemical properties of the waste and other surrounding conditions, which makes optimization of the process difficult. The most promising options to counter this massive generation of waste are eco-friendly treatments or recycling. As a preliminary step for these options, attempts were made to evaluate the feasibility and usability of three simulative models based on reaction kinetics. Model (A) predicted relative changes over reaction time for reactant, intermediate, and product. Overall, an increased reaction rate produced less intermediate and more product, thereby leading to a shorter total reaction time. Particle diminishing model (B) predicted reduction of the total waste mass. The smaller particles diminished faster along with the dominant effect of microbial reaction. In Model (C), long-chain cellulose was predicted to transform into reducing sugar. At a standard condition, 48% of cellulose molecules having 10⁵ repeating units turned into reducing sugar after 100 h. Also it was found that the optimal enzyme concentration where the highest amount of remnant sugar was harvested was 1 mg L^-1.

• 유기물자원화
• /
• 제14권3호
• /
• pp.117-125
• /
• 2006

원유에 오염된 토양으로부터 톨루엔을 분해할 수 있는 미생물을 분리하였으며 동정결과 Pseudomonas fluorescence였다. 이 미생물을 생물학적 톨루엔 처리공정에 사용하기 위해 온도, 톨루엔 농도, pH, 질소원 등의 환경영향이 분해에 미치는 영향에 대해서 연구를 하였다. 이 미생물의 최적 분해 온도는 $30^{\circ}C$였으며 이 때 최대 비성장속도와 최대 비소모속도는 각각 $0.76hr^{-1}$와 $0.36hr^{-1}$이었다. $10^{\circ}C$ 와 $40^{\circ}C$에서는 톨루엔을 분해하지 못하였으나 $30^{\circ}C$에 적응한 미생물은 $10^{\circ}C$에서 17시간 만에 100mg/L의 톨루엔을 완전히 분해하였고 $40^{\circ}C$ 에서는 30시간 동안 80% 정도 분해할 수 있었다. 본 미생물은 200mg/L 이상의 톨루엔은 분해할 수 없었으나 20mg/L의 낮은 농도의 톨루엔에 적응을 시킴으로써 300mg/L의 톨루엔까지 분해할 수 있었다. pH는 5.5~9.0 범위에서 분해 속도에 별다른 영향을 미치지 않는 것으로 나타났다. 암모늄 (${NH_4}^+$) 대신 질산염(${NO_3}^-$)을 사용했을 때 적응기가 2~10시간 정도 길어졌고 미생물 수율이 45% 정도 감소하였다. 그러나 적응기가 지난 후의 톨루엔 분해속도에서는 별 차이를 보이지 않았다. 본 연구에서 얻은 결과들은 향후 바이오필터 등의 생물학적 톨루엔 처리공정 개발에 유용하게 사용될 것으로 기대된다.