• Title/Summary/Keyword: L-Shaped

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The Effect of Electrical Characteristics and Electrode Shape on Alignment of Multi-walled Carbon Nanotubes (전기장 특성과 전극 형상이 다중벽 탄소나노튜브 정렬에 미치는 영향)

  • Kwon, Se-Hun;Jeong, Young-Keun;Jung, Chang-Sik;Kang, Myung-Chang;Lee, Hyung-Woo
    • Journal of Powder Materials
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    • v.17 no.4
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    • pp.326-335
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    • 2010
  • In this paper, the effect of electrical characteristics and electrode shape on the alignment and attachment of multi-walled carbon nanotubes (MWNTs) has been studied. The attraction and alignment of MWNTs between the gaps has been investigated by applying electric field which is called electrophoresis and dielectrophoresis. According to the frequency of electric field, positive or negative dielectrophoretic force can be determined. The concentration of MWNTs solution was $5\;{\mu}g/ml$, and a droplet of $1.0{\sim}1.5\;{\mu}l$ was dropped between two electrodes. Through the repeated experiments, the optimal electrical conditions for aligning and attaching MWNTs in the desired places were obtained. Since the frequency range of 100 kHz~10 MHz generated positive dielectrophoretic force, MWNTs were attracted and aligned between the gaps with this frequency range. For generating enough force to attract MWNTs, the appropriate voltage range was $0.3{\sim}1.3\;V_{rms}/{\mu}m$. Furthermore, the effect of electrode shape on the alignment of MWNTs was investigated. A single MWNT attachment was accomplished on the round shaped with 70% yield.

Analysis of Genes with Alternatively Spliced Transcripts in the Leaf, Root, Panicle and Seed of Rice Using a Long Oligomer Microarray and RNA-Seq

  • Chae, Songhwa;Kim, Joung Sug;Jun, Kyong Mi;Lee, Sang-Bok;Kim, Myung Soon;Nahm, Baek Hie;Kim, Yeon-Ki
    • Molecules and Cells
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    • v.40 no.10
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    • pp.714-730
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    • 2017
  • Pre-mRNA splicing further increases protein diversity acquired through evolution. The underlying driving forces for this phenomenon are unknown, especially in terms of gene expression. A rice alternatively spliced transcript detection microarray (ASDM) and RNA sequencing (RNA-Seq) were applied to differentiate the transcriptome of 4 representative organs of Oryza sativa L. cv. Ilmi: leaves, roots, 1-cm-stage panicles and young seeds at 21 days after pollination. Comparison of data obtained by microarray and RNA-Seq showed a bell-shaped distribution and a co-lineation for highly expressed genes. Transcripts were classified according to the degree of organ enrichment using a coefficient value (CV, the ratio of the standard deviation to the mean values): highly variable (CVI), variable (CVII), and constitutive (CVIII) groups. A higher index of the portion of loci with alternatively spliced transcripts in a group (IAST) value was observed for the constitutive group. Genes of the highly variable group showed the characteristics of the examined organs, and alternatively spliced transcripts tended to exhibit the same organ specificity or less organ preferences, with avoidance of 'organ distinctness'. In addition, within a locus, a tendency of higher expression was found for transcripts with a longer coding sequence (CDS), and a spliced intron was the most commonly found type of alternative splicing for an extended CDS. Thus, pre-mRNA splicing might have evolved to retain maximum functionality in terms of organ preference and multiplicity.

Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus)

  • Lee, Jong Kyu;Kim, Hak Jun
    • Fisheries and Aquatic Sciences
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    • v.19 no.8
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    • pp.33.1-33.7
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    • 2016
  • Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5'-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of $0.07{\pm}0.01^{\circ}C$ at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

Morphological Characteristics and Life cycle of Udea ferrugalis (Lepidoptera, Pyralidae) Attacking Angelica utitis (신선초를 가해하는 주홍날개들명나방(Udea ferrugalis)의 형태적 특징 및 생활사)

  • 이기열;민경범;안기수;최관순;장영덕;윤영남
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.109-115
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    • 1999
  • Morphological characteristics and life cycle of Udea ferrugalis (Hubner) attacking Angelica utilis were studied in 1995 and 1996. Egg of U. ferrugalis was oval-shaped with light-gray. Larva was milk white or light yellow color with 1.50-14.6 mm body length, depending on their developmental stages from 1st to 5th instar. Pupa were deep-brown with 8.2-9.7 mm length. Adults were brown color and body lengths were 10.1 mm and 8.3 mm and wing expanse lengths were 18.5 mm and 16.6mm with female and male, respectively. At 20$^{\circ}$C, each developmently periods was 5.81, 19.0, 3.46 and 1 l.0days with egg, larvae, pre-pupae, and pupae, respectively. Also adult longevitys were 10.13 for female and 9.8 days for male. Average number of egg were 238.5 and 248.8 at 20$^{\circ}$C and 30$^{\circ}$C, respectively. A total of 12 species host plants were identified in the outdoor observation in Chungbuk province.

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CLINICAL STUDY ON USE OF BIODEGRADABLE PLATE AND SCREW IN ORTHOGNATHIC SURGERY (턱교정 수술에 있어 흡수성 고정판 및 나사 사용에 대한 임상적 연구)

  • Park, Sung-Soo;Choi, Jin-Young
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.2
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    • pp.127-135
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    • 2009
  • Objectives : The titanium fixation system has been used in orthognathic surgery for fixation of bone segments usually, but the biodegradable fixation system was developed and also being used. The strongest point in the biodegradable system is that no extra operation should be needed to remove fixation materials. In spite of this merit, oral & maxillofacial surgeons hesitate to use this system in fracture or orthognathic surgery. In this study, as we got some clinical experiences, we'd like to report the result of clinical study using the biodegradable fixation system in orthognathic surgery. Patients and Methods : A total of 35 patients composed of 17 males and 18 females with 25 osteotomies in maxilla and 34 osteotomies in mandible were fixated with the biodegradable fixation system(Inion $CPS^{(R)}$). We investigated methods of stabilization, fixation time, and complications on the basis of the method as above. Results : Four 2mm thick L shaped plates with 7 holes of which 1 hole was removed were fixed in maxilla with six $2.0{\times}7mm$ screws. Three $2.5{\times}16{\sim}18mm$ screws were used to fix superior ramus area and one mandibular angle area in mandible. It took about 27.4 minutes in maxilla, 25.3 minutes in mandible to perform the fixation which took longer time than the titanium system(9.5 minutes in maxilla, 8 minutes in mandible). Generally, there was no problem except 9 cases in which there were some complications. Conclusions : In most cases, the biodegradable fixation system can be used without problem in usual orthognathic surgery. But, this system is inferior to the titanium fixation one in some respects such as fixation time, size, and physical property. Some supplementations for such weak points as aforementioned should be needed for the universal use of biodegradable materials.

A femtosecond Cr:LiSAF laser pumped by semiconductor lasers (반도체 레이저 여기 펨토초 Cr:LiSAF 레이저)

  • 박종대
    • Korean Journal of Optics and Photonics
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    • v.11 no.5
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    • pp.360-364
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    • 2000
  • We demonstrate self-starting passIve mode locking of a Cr:LiSAF laser, using a SCIDlconduclor Saturable Absorber Mirror (SESAM), Two high-power red semiconductor lasers (Coherent S-67-500C-100-H) of wavelength 667 nm and maximum power of 500 mW were used as pump lasers, The cavity has 10 cm radius-ai-curvature folding minors, two SF 10 prisms, a 99% reflectivity output coupler and a SESAM at dIe focus of a 10 cm radIus-at-curvature mirror. We used the laser crystal in BrewsterBrewster shape with 1 5% $Cr^{+3}$ ion concentration and the length of 6 mm, An X-shaped resonator was used to compensate the astigmatism induced by tile crystal. The structure of the SESAM cOllSists of 30 pmr of $AlAs/Al_{0.15}Ga_{0.85}As$ layer, wi1l1 a 10 nm GaAs quantum well situated in the topmost layer Output spectra were centeled at 833 nm, with 4 nm spectral bandwidth and pulse width was measured to be 220 fs, Output power of 3 mW is obtained at a pump power of 800 mW. 00 mW.

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CT Classification and Treatment of Intraarticular Calcaneal Fractures - Conservative vs. Surgical Treatment by Prospective Study - (관절내 종골 골절의 전산화 단층 촬영에 따른 분류 및 치료)

  • Kang, Jae-Do;Kim, Hyung-Chun;Kim, Jin-Hyung
    • Journal of Korean Foot and Ankle Society
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    • v.5 no.1
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    • pp.35-42
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    • 2001
  • Purpose: To analyze the results of conservative or surgical treatment after computed tomograhy(CT) classification in intraarticular calcaneal fractures. Materials and methods: From January 1996 to May 1999, we prospectively analyze 23 cases of intraarticular calcaneal fractures who were treated conservatively or operated by open reduction and internal fixation by extensive L-shaped lateral approach after CT classification. Results: A functional scoring system of 0-100 points which was based upon the responses to AOFAS Ankle-Hindfoot Scale for the operative group was at 82.8, compared with 73.2 for the non-operative group, and these were meaningful statistically(P<0.05). Of type I fracture, in the operative group there were 2 excellent results and in the non-operative group there were 2 excellent results, 1 good result. or type II fractures, in the operative group there were 2 excellent results, 3 good results, 1 fair result and in the non-operative group there were 1 good result, 1 fair result, 2 poor results. Of type III fractures, in the operative group there were 2 fair results, 2 poor results and in the non-operative group there were 1 fair result, 3 poor results. Bohler angles of subtalar joint were changed from initial average $13.3^{\circ}$ to postoperative average $20.9^{\circ}$ for the operative group compared with from initial average $15.5^{\circ}$ to follow-up average $14.8^{\circ}$ of the non-operative group(P<0.01). Conclusions: Computed tomography in the evaluation of intraarticular calcaneal fractures is effective tool. We believed that open reduction and internal fixation in all Crosby & Fitzgibbons type II and according to degrees of comminution reducible type III for the intraarticular calcaneal fractures is more effective method than conservative treatment.

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Development of Adaptive Moving Obstacle Avoidance Algorithm Based on Global Map using LRF sensor (LRF 센서를 이용한 글로벌 맵 기반의 적응형 이동 장애물 회피 알고리즘 개발)

  • Oh, Se-Kwon;Lee, You-Sang;Lee, Dae-Hyun;Kim, Young-Sung
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.13 no.5
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    • pp.377-388
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    • 2020
  • In this paper, the autonomous mobile robot whit only LRF sensors proposes an algorithm for avoiding moving obstacles in an environment where a global map containing fixed obstacles. First of all, in oder to avoid moving obstacles, moving obstacles are extracted using LRF distance sensor data and a global map. An ellipse-shaped safety radius is created using the sum of relative vector components between the extracted moving obstacles and of the autonomuos mobile robot. Considering the created safety radius, the autonomous mobile robot can avoid moving obstacles and reach the destination. To verify the proposed algorithm, use quantitative analysis methods to compare and analyze with existing algorithms. The analysis method compares the length and run time of the proposed algorithm with the length of the path of the existing algorithm based on the absence of a moving obstacle. The proposed algorithm can be avoided by taking into account the relative speed and direction of the moving obstacle, so both the route and the driving time show higher performance than the existing algorithm.

Renal Lymphoma with Mesenteric Lymphomatosis in a Cat

  • Jeon, Jin-ha;Song, Doo-won;Ro, Woong-bin;Kim, Heyong-seok;Lee, Ga-won;Cho, Jun-ho;Jeong, Woo-chang;Kim, Soo-hyeon;Sur, Jung-hyang;Park, Hee-myung
    • Journal of Veterinary Clinics
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    • v.37 no.4
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    • pp.208-212
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    • 2020
  • A 6-year-old castrated male Russian Blue cat was presented for evaluation of dyschezia. Abdominal ultrasound revealed hyperechoic nodules in both kidneys, heterogeneous mass in abdomen, and extensive mesenteric thickening with multiple hypoechoic nodules. Computed tomography showed multiple hypodense lesions in both kidneys and diffuse nodular infiltration around the mesentery. Fine needle aspirates (FNA) acquired under ultrasound guidance from the mesentery consisted of large lymphocytes which have round to irregular nuclei with granular chromatin, prominent nucleoli and a small amount of basophilic cytoplasm. Polymerase chain reaction (PCR) for antigen receptor gene rearrangement result of FNA sample revealed a T-cell malignancy. The cat died from acute renal failure after 1 cycle of modified Madison-Wisconsin L-CHOP protocol. Postmortem examination revealed bilaterally enlarged lumpy-bumpy shaped kidneys. Histopathologic examination showed an infiltration of malignant lymphocytes into the renal parenchyma and mesentery. Immunohistochemical staining of the renal sample displayed a negative expression of CD3, PAX5, MUM-1, and CD79. The clinical features and prognosis of the cat with renal lymphoma with mesenteric lymphomatosis have been described in this report.

Isolation and Microbiological Characterization of Azospirillum from the Rhizosphere of Oryza sativa L. in Korea (우리나라 벼 근권으로부터 분리한 Azospirillum 균주의 미생물학적 특성)

  • Kim, Won-Gon;Seo, Hyun-Chang;Kim, Jong-Pyung;Kim, Chang-Jin;Lee, Ke-Ho;Yoo, Ick-Dong
    • Korean Journal of Microbiology
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    • v.32 no.2
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    • pp.97-101
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    • 1994
  • Fifteen strains of the nitrogen fixer Axospirillum were isolated from the rhizosphere of rice collected from Kyonggi-do and Chungcheongnam in Korea. They had strong acetylene-reducing activity of 400 of 900 nmol $C_2H_4$ per hour vial had a similar morphology in succinate-malate medium: vibrioid cells having a diameter of 1.0 ${\mu}m$ and a monopolar single flagellum in liquid media. According to their physiological and morphological characteristics, they were divided into two distinct groups, group I and group II. Group I strain were, unlike group II, distinguished by their ability to use glucose as a sole carbon source in nitrogen-free medium, requirement for biotin, and formation of wider, longer, and S-shaped cells in semisolid nitrogen-free malate medium. On the basis of their characteristics, strains belonging to group I were identified as Azospirillum lipoferum, while those belonging to group II were identified as Azospirillum brasilense.

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