• 약학회지
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• 제31권4호
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• pp.230-235
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• 1987

Antitumor and antimicrobial activities of water soluble fractions of Duchesnea indica (Andr.) and Focke (Rosaceae), have been studied. The contents of total polysaccharide were 22% and its composition was identified by G.C.: L-arabinose(Rt: 7.71), D-xylose (Rt. 9.91), D-glucuronic acid (Rt. 12.77), D-fructose (Rt. 13.63), D-galactose (Rt. 14.68), D-glucose (Rt. 15.42). The antimicrobial activity of Duchesnea indica against four bacterial species, S. aureus, S. paratyphi, S. dysenteriae and Ps. aeruginosa was observed.

• 한국미생물·생명공학회지
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• 제27권3호
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• pp.260-265
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• 1999

The structural $\beta$-galactosidase gene (lacZ) from Lactococcus lactis ssp. lactis 7962 was cloned into plamid vector pKF18, which was designated as pKF-gal. Expression of the lacZ from L. lactis 7962 was found to be higher when cells were grown at 3$0^{\circ}C$ than 37$^{\circ}C$. Maximum $\beta$-galactosidase activity was obtained when E. coli/pKF-gal was cultivated for 6hr at 3$0^{\circ}C$ and for 3hr at 37$^{\circ}C$, and L. lactis 7962 was grown for 8hr at 3$0^{\circ}C$. Enzyme induction was achieved by the addition of lactose, galactose, or lactose+IPTG to growing culture. The addition of glucose had no effect on enzyme induction.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.204.2-204.2
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• 2003

The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cytotoxic and immune-system-stimulating activities. Korean mistletoe (Viscum album L. coloratum), a subspecies of European mistletoe, has also been reported to possess anticancer and immunological activities. A galactose- and N-acetyl-D-galactosamine- specific lectin (Viscum album L. coloratum agglutinin, VCA) with Mr 60 kDa was isolated from Korean mistletoe. (omitted)

• KSBB Journal
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• 제29권5호
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• pp.307-315
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• 2014

Recently seaweed polysaccharides have been extensively studied for alternative energy application. Because their producing cost is high and efficiency low, their industrial applications have been limited. The main component of cell wall of red algae represented by Gelidiales and Gracilariales is agar. Red-algae agar or galactan, consisting of D-galactose and 3, 6-anhydro-L-galactose, is suitable for bio-product application if hydrolyzed to monomer unit. For the hydrolysis of algae, chemical or enzymatic treatment can be used. A chemical process using a strong acid is simple and efficient, but it generates together with target sugar and toxic compounds. In an enzymatic hydrolysis process, target sugar without toxic compounds generation. The objective of this review is to summary the recent data of saccharification by chemical and enzymatic means from red seaweed for especially focused on automobile industry.

• 한국식품영양과학회지
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• 제11권2호
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• pp.21-25
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• 1982

나무딸기 과즙색소에 대한 당류의 안정화 효과를 조사한 결과는 다음과 같다. 1. 과즙의 당도, pH,적정산도는 저온구, 냉동구에서 80일간 저장 기간중 거의 변화가 없었으며 색소변화에 영향을 미치지 않았다. 2. 과즙의 색조(色調)와 색소액(色素液)의 저장중 변화는 시간이 경과함에 따라 열화(劣化)되었으며 색소액의 변화는 색조보다 적었다. 3. 당류 첨가에 의한 색소액의 농색화 효과는 육탄당(六炭糖)이 가장 좋았으며 이 중에서도 D-galactose가 우수하였다. 그 다음 이당류(二糖類)였고 오히러 색소를 파괴시키는 것은 D-xylose와 L-rhamnose 였다. 4. 당 첨가에 의해 농색화된 색소액은 저장중 온도에 크게 영향을 받았으며 특히 저온구$(5^{\circ}C)$에서 거의 안정하게 보존되었다.

• 한국수산과학회지
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• 제53권5호
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• pp.665-671
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• 2020

The chemical and rheological properties of fucoidan and alginate prepared from different parts of Undaria pinnatifida (sporophyll, frond, stipe) were investigated. The algal materials were extracted with HCl (pH 2.0, 3 h at 70℃) to prepare fucoidan, and the remaining solid was continuously re-extracted with Na₂CO₃ (pH 10.0, 70℃, 3 h) to prepare alginic acid. The fucoidan and alginic acid contents in the sporophyll, frond, and stipe were 11.14%, 3.84%, and 1.73% and 22.04%, 37.14%, and 31.74%, respectively. The content of fucoidan and alginate depends on the part extracted. The fucoidan extracted from the sporophyll mainly consists of fucose and galactose, but the fucoidan extracted from frond and stipe contains mannose in addition to fucose and galactose. Fourier-transform infrared spectroscopy analysis of fucoidan and alginate suggests the presence of sulfate groups (1261 and 840 cm^-1) and carboxyl groups (1626 and 1419 cm^-1), respectively. Alginate solutions (5%) had a low viscosity of 10.84-31.63 mPa·s. The activation energies of fucoidan and sodium alginate were 14.45-18.38 kJ/mol and 18.61-22.06 kJ/mol, respectively. The D-mannuronic acid/L-guluronic acid (M/G) ratios of alginate showed a relatively high (frond, 3.72; stipe, 2.88; and sporophyll, 1.80).

• Food Science and Biotechnology
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• 제17권4호
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• pp.839-841
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• 2008

The mutagenicity after heating of different sugars (glucose, fructose, galactose, and tagatose) on the non-enzymatic browning reaction in different sugars and glycine model system was investigated. The model system containing 0.2 M glycine and 0.2 M of different sugars in 10 mL water was heated at $150{\pm}5^{\circ}C$ for 30 min. After heating, degree of non-browning reaction intensity and mutagenicity using Salmonella typhimurium TA 98 were examined. Heated glycine model systems containing different sugars increased their mutgenicity ranged from 30 to 372 revertant colonies. After heating for 40 min, mutagenicity was achieved with glycine model systems containing 4 different sugars with by 145, 356, 206, and 369 revertants per plate, respectively. The glycine model systems containing fructose or tagatose were significantly (p<0.05) higher mutagenic activity than glycine model systems containing glucose or galactose after 40 min of heating. The linear regression between Maillard reaction intensity and mutagenic activities (slope=32.38, $R^2=0.93$) indicates that mutagenicity could be fully ascribed to Maillard reaction products.

• 한국미생물·생명공학회지
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• 제36권1호
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• pp.61-66
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• 2008

Flask 배양으로 기본 유청 배지MSM를 배지로 하고 첨가한 각 탄소원의 PS-7생산 효과를 살펴보면 fructose, glucose와 sucrose의 첨가가 PS-7의 생산을 다른 당에 비해 향상시킴을 알 수 있었다. 그러나 galactose의 경우 첨가량이 증가될수록 오히려 PS-7의 생산량이 감소되는 경향을 나타내었다. 여러 가지 배양 변수를 고려한 결과 glucose씨 첨가가 최적임을 확인하였다. 5L 발효조에 의한 PS-7 생산의 경우 유청과 glucose를 함께 첨가한 것이 유청과 glucose만을 배지의 탄소원으로 사용했을 때 보다 PS-7의 생산량이 월등히 향상되는 것을 알 수 있었으며, 유청 1%와 glucose 1%배지의 경우 PS-7생산량이 3 g/L를 생산하였다. 유청 1%와 glucose 2%배지의 경우 4 g/L의 PS-7의 생산하였으나 잔당량이 $30{\sim}40%$가 남아 생산효율(yield)면에서는 떨어짐을 알 수 있었다. 배지내 Glucose농도 변화에 따른 점성(viscosity)의 변화는 PS-7의 생산량의 변화와 비슷한 패턴을 보이지만 분자량 증대에 따른 점도의 증가는 훨씬 높게 나타났다. 본 실험의 결과 일반적으로 C/N ratio가 다당류인 PS-7생산에 중요한 역할을 하고 있으며, 유청 1%+glucose 1% 배지를 사용할 때 높은 점도와 수율의 PS-7을 얻을 수 있는 최적 배지임을 알 수 있었다.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.18-25
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• 2023

After random mutagenesis, the mutant Lactobacillus plantarum GNS300 showed improved exopolysaccharide production as determined by the quantification of total sugar. The mutant L. plantarum GNS300 produced 2.82 g/l of exopolysaccharide which showed 79.62% improved exopolysaccharide production compared with the parental strain. When exopolysaccharide of L. plantarum GNS300 was analyzed, the exopolysaccharide is composed of galactose (93.35%) and glucose (6.65%). Through the optimization of fermentation conditions using a bioreactor, 2.93 g/l of exopolysaccharide was produced from 20 g/l of glucose at 35℃, 500 rpm, and 0.1 vvm for 12 h. The mutant L. plantarum GNS300 exhibited 69.18% higher antioxidant activity than that from the parental strain, which might be caused by higher exopolysaccharide production. The concentrated supernatant of the mutant L. plantarum GNS300 inhibited the growth of gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli, Vibrio parahaemolyticus, and Salmonella typhimurium).

• 한국식품과학회지
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• 제27권6호
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• pp.878-884
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• 1995

본 연구는 유청을 에탄올 발효에 이용하기 위하여 유청에 함유된 유당을 ${\beta}-D-galactosidase$로 가수분해한 후 Saccharomyces cerevisiae와 Kluyveromyces fragilis의 발효 조건을 비교하였으며, 효모의 특성인 pasteur effect를 이용하여 용존 산소의 조절에 의한 유청에서의 K. fragilis와 S. cerevisiae의 에탄올 발효 조건을 실험하였다. 또한 탁주의 제조에 에탄올 발효된 유청을 용수에 혼합, 응용하기 위한 최적 조건을 조사하였다. pH6.5로 조절된 유청에 0.7%(v/v)의 ${\beta}-D-galactosidase$를 첨가하여 30분만에 93%의 유당이 가수분해 되었다. 이를 배지로 이용하여 에탄올 발효한 결과 S. cerevisiae는 galactose를 이용하지 못하므로 에탄올 생산력이 K. fragilis에 비하여 낮았으나 glucose를 첨가한 배지에서는 S. cerevisiae의 에탄올 생산량이 K. fragilis 보다 증가하였다. 용존 산소를 조절하고 glucose를 첨가하여준 실험에서는 K. fragilis와 S. cerevisiae의 에탄올 생산량이 각각 18.9 g/l와 34.5 g/l로 에탄올 생산량이 11.8% 22.1% 증가하였다. 즉 용존산소를 조절한 조건에서는 S. cerevisiae의 에탄올 생산력이 더 우수하였다. 미분을 첨가한 에탄올 발효에서는 Aspergilus oryzae가 발효 60시간만에 80% 이상의 당화력을 보였으며, 이때 생산되는 에탄올의 양은 80.2 g/l였다.