Objectives: To assess the scavenging effect of carnitine derivatives on oxidative damage to sperm during sperm processing, cryopreservation and thawing. Materials and Methods: Fresh semen samples from 20 normal healthy volunteers were collected by masturbation after at least 48 hours abstinence. After liquefaction of semen samples at room temperature, the specimens were diluted with sperm wash media (Ham's F-10, Life technologics) to a uniform density of $20{\times}10^6/ml$. L-carnitine or acetylcarnitine were added with various concentration of $0{\mu}M$, $10{\mu}M$, $30{\mu}M$ in semen sample or cryoprotectant. All specimens were cryopreservated at $-196^{circ}C$$LN_2$ for 3 days. Sperm motility, vitality, fertilizing capacity, reactive oxygen species formation and the level of lipid peroxidation were analyzed by computer assisted semen analyzer, eosin-nigrosin stain, hypoosmotic swelling test, chemiluminescence and thiobarbituric acid method, respectively, during sperm processing, cryopreservation and thawing. Results: The sperm motility was only increased in proportion to the concentration of acetylcarnitine with no statistical significance (p>0.05). The sperm vitality was also significantly improved in proportion to the concentration of acetylcarnitine with statistical significance (p<0.05). The sperm fertilizing capacity was significantly increased in proportion to the concentration of L-carnitine and acetylcarnitine and reactive oxygen species generation and lipid peroxidation were significantly decreased with same fashion (p<0.05). On comparison of effects between L-carnitine and acetylcarnitine, acetylcarnitine was superior to L-carnitine on the improvement of sperm motility and vitality as well as the suppression of reactive oxygen species generation and lipid peroxidation. Conclusions: These results suggest that carnitine derivatives have a scavenging effect against oxidative damages during sperm processing, cryopreservation and thawing. Therefore, carnitine derivatives may be useful as an oral antioxidant in patients with male infertility due to increased ROS generation.
Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17℃. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17℃.
Manee-In, S.;Parmornsupornvichit, S.;Kraiprayoon, S.;Tharasanit, T.;Chanapiwat, P.;Kaeoket, K.
Asian-Australasian Journal of Animal Sciences
/
v.27
no.6
/
pp.791-796
/
2014
Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examined.
This was conducted to determine the effects of body weight, organ weight, hematological values and biochemical parameters by L-carnitine in the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group (n=10) received operation and no treatment. OVX+Carn group (n=10) received operation and L-carnitine. Body weight was significantly lower in OVX+Carn group than in all other groups. Also, organ weight such as heart, liver, spleen and kidney was measured. The heart and spleen weight were significantly lower in the OVX+Carn group than in the Intact and Sham group. The liver weight in the OVX+Carn group was significantly differences in comparison with those in the other groups. Also, there was significantly differences in the organ weight of kidney between in the OVX+Carn group and in the other groups. The hematological values of WBC, RBC, MCV, MCH and MCHC were no significant differences in any other groups. The total cholesterol, triglyceride and high density lipoprotein decreased significantly in the OVX+Carn group as compared to those in the OVX group. But, there were no significant differences in low density lipoprotein in any other groups. We conclude that L-carnitine enhanced the body weight in the ovariectomized rats. Our findings suggest that L-carnitine may influence the process of absorption of fat in the ovariectomized rats.
This experiment was carried out to determine the effects of supplementation of L-carnitine and humic substances alone or in combination in laying hen diets on performance, egg traits and blood parameters. A total of 180 IGH type brown laying hens aged 22 weeks were employed in a completely randomized block design with one control group and three treatment groups. Each group was divided into five replicates as subgroups, each comprising 9 hens. The diets of the first, second and third treatment groups were supplemented with 0.1 g/kg L-carnitine, 1.5 g/kg humic substances (Farmagulator$^{(R)}$ Dry Plus) and 0.1 g/kg L-carnitine+1.5 g/kg humic substances, respectively. The experimental period lasted 18 weeks. Feeding supplemental carnitine, humic substances or carnitine+humic substances resulted in increases in body weight gain (p<0.05). Dietary treatments did not significantly affect daily feed intake, daily metabolizable energy intake, egg production, egg weight, feed efficiency, mortality, egg shape index, egg breaking strength, egg shell thickness, egg albumen index, egg yolk index, egg Haugh unit and the percentages of egg shell, albumen and yolk. Supplementation of humic substances reduced egg yolk cholesterol as mg per g yolk and mg per yolk (p<0.05). Blood serum parameters were not affected by the supplementation of carnitine, humic substances or carnitine+humic substances. The results in this study demonstrated that humic substances supplementation reduced egg cholesterol without adverse effects on performance, egg traits and blood parameters of laying hens. It was concluded that the usage of L-carnitine alone or in combination with humic substances in diets had no beneficial effects in laying hens.
L-Carnitine is an antioxidant for the transport of fatty acids in mitochondria and breakdown of lipids for metabolic energy. Some studies have suggested that carnitine improves sperm motility in mammals. The objective of this study was to investigate the effect of L-carnitine on the characteristics in fresh semen of miniature pigs. The collected fresh semen was stored in modena B medium with L-carnitine (0, 1.0, 2.0, and 4.0 mg/ml) for 10 days at $18^{\circ}C$. The semen quality of viability, acrosome reaction and mitochondria integrity was analyzed on 0, 3, 7, and 10 day of semen storage. The percentages of live and dying sperm were not different among treatment groups with different concentrations of L-carnitine during the storage period. In acrosome reaction analysis, when the sperm stored for 7 day, the percentages of live sperm with acrosome reaction were significantly (p<0.05) lower in 1 ($9.0{\pm}0.9%$), 2 ($7.6{\pm}0.2%$) or 4 mg/ml ($7.9{\pm}0.8%$) L-carnitine-treated groups than the control group (0 mg/ml L-carnitine) ($11.12{\pm}0.2%$). However, there were no difference in percentages of live sperm with acrosome reaction for 3 and 10 days of storage with each concentrations of L-carnitine. When sperm was stored for 3 and 10 days, the percentages of live sperm with mitochondria integrity were significantly higher in 2 mg/ml of L-carnitine-treated group than control group (p<0.05). In conclusion, the L-carnitine has a positive effect on acrosome reaction and mitochondria integrity in liquid state of fresh semen in miniature pigs.
This study evaluated the effects of carnitine supplementation on obesity caused by a high-fat diet in C57BL/6J mice. The mice were fed a normal diet (ND), high-fat diet (HD), or carnitine-supplemented (0.5% of diet) high-fat diet (HDC) for 12 weeks. The results showed that body weight, energy intake, and feed intake were lower in the HDC group than the control groups. Acid-soluble acylcarnitine (A SAC), acid-insoluble acylcarnitine (AIAC), and total carnitine (TCNE) in the serum and liver were significantly higher in the HDC group. Hepatic carnitine palmitoyl transferase-I activity was significantly higher in the HDC group than the control groups. Acyl-coA synthetase (ACS) and carnitine palmitoyl transferase-I (CPT-I) mRNA expression in the liver was highest in the HDC group, however hepatic acetyl-coA carboxylase (ACC) mRNA expression in this group was lowest. Serum leptin levels and abdominal fat weight were lowest in the HDC group. We concluded that L-carnitine supplementation diminished the risk of obesity caused by a high-fat diet.
This study is to investigate the effect of dietary L-carnitine supplementation on lipid metabolism in rats fed with isolated soy protein and casein for their source of protein. Four experimental groups were organized and each group had eight Sprague-Dawley male rats with the initial weight of around 180g. The four groups were CO (casein only supplemented group); CC (casein and 3% L-carnitine supplemented group); ISO (isolated soy protein only supplemented group); ISC (isolated soy protein and 3% L-carnitine supplemented group). All groups were supplemented with the experimental diet for four weeks and carnitine comprised 3% of. their diet. The results were as follows; 1. There was no significant difference in food intake among the groups. 2. Final weight gain was significantly lower in the groups supplemented with isolated soy protein than in the groups supplemented with casein (P<0.05). The groups with supplemented casein and carnitine showed the effect of weight reduction (p<0.05). 3. Food efficiency ratio was lower in the groups supplemented with isolated soy protein than in the groups supplemented with casein (p<0.01). The groups supplemented with casein and carnitine showed low food efficiency ratio. 4. The serum total lipid was higher in the groups supplemented with casein than in the groups supplemented with isolated soy protein (p<0.05). 5. Serum total cholesterol was higher in the groups supplemented with casein than in the groups supplemented with isolated soy protein. 6. There was no significant difference in triglyceride, HDL-cholesterol, and LDL-cholesterol in serum among the groups. 7. Out of the groups supplemented with isolated soy protein the total cholesterol level in liver was low in the groups to which carnitine was supplemented (p< 0.05). However, there was no significant difference of liver total lipid and triglyceride among the groups. 8. There was no difference in TBARS levels and GSH-Px activities in liver among the groups.
Du, Rong;Qin, Jian;Wang, Jundong;Pang, Quanhai;Zhang, Chunshan;Jiang, Junfang
Asian-Australasian Journal of Animal Sciences
/
v.18
no.2
/
pp.235-240
/
2005
Two hundred and eighty-eight 21-week-old Hyline Brown laying hens were randomly allotted to 9 treatments, 32 birds for each treatment. A 3${\times}$3 (chromium${\times}$L-carnitine) factorial experiment was designed to investigate the single and interactive effects of adding yeast chromium (0, 400 and 600 ${\mu}g/kg$) and L-carnitine (0, 50 and 100 mg/kg) to corn-soybean diets on lipid metabolism of laying hens for 7 weeks. The results showed that 600 ${\mu}g/kg$ chromium or 100 mg/kg L-carnitine had significant effects on most indices of lipid metabolism (p<0.05 or 0.01). There were significant interactions on the concentration of liver triglycerides, egg yolk cholesterol, abdominal fat percentage between chromium and L-carnitine (p=0.0003-0.0500). Adding 400 ${\mu}g/kg$ chromium and 100 mg/kg Lcarnitine simultaneously was the best for reducing egg yolk cholesterol and adding 400 ${\mu}g/kg$ chromium and 50 mg/kg L-carnitine at the same time was the best for reducing abdominal fat percentage. There was no side effect on production performance of laying hens while chromium or (and) L-carnitine reduced liver lipid, abdominal fat and egg yolk cholesterol.
Objective: The genes Bcl-2 and Bax play important roles in apoptosis. Many studies have shown that formalin has a strong deleterious effect on male fertility and can induce apoptosis. L-carnitine has been reported to potentially reverse the negative effects of formalin, leading to improved spermatogenesis. In this study, we examined the levels of expression of Bcl-2 and Bax in mice treated with formalin and L-carnitine. Methods: Thirty adult BALB/c mice were categorized into three groups. The mice in the control group (n = 10) were not injected with any substance. The mice in the second group (n = 10) received 10 mg/kg of formalin daily via an intraperitoneal injection, while those in the final group (n = 10) were intraperitoneally injected daily with a dose of 10 mg/kg of formalin and 100 mg/kg of L-carnitine. All mice were kept in isolated cages for 31 days. Results: The expression of Bax was significantly higher in the formalin-treated mice than in the mice of the control group, while the expression of Bcl-2 was significantly lower in the formalin-treated mice than in the control mice. Additionally, relative to control mice, Bcl-2 expression increased and Bax expression decreased in the mice administered both formalin and L-carnitine. Conclusion: In this study, L-carnitine was shown to augment Bcl-2 expression and to reduce Bax expression, indicating that this compound may inhibit apoptosis. Due to its positive effects, L-carnitine can be used as a prophylactic treatment for people who routinely come into direct contact with formalin as an occupational hazard.
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