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http://dx.doi.org/10.5713/ajas.19.0455

Effect of L-carnitine on sperm quality during liquid storage of boar semen  

Yang, Kang (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Wang, Na (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Guo, Hai-Tao (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Wang, Jing-Ran (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Sun, Huan-Huan (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Sun, Liang-Zhen (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Yue, Shun-Li (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Zhou, Jia-Bo (Department of Biotechnology, College of Life Science Northeast Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.33, no.11, 2020 , pp. 1763-1769 More about this Journal
Abstract
Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17℃. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17℃.
Keywords
Boar; Sperm; L-carnitine; Liquid Storage; Antioxidant Capacity;
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