• Title/Summary/Keyword: Kyeongbuk

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Stabilization of Compact Protein Structures by Macrocyclic Hosts Cucurbit[n]urils in the Gas Phase

  • Lee, Jong Wha;Park, Mi Hyun;Ju, Jeong Tae;Choi, Yun Seop;Hwang, Soo Min;Jung, Dong Jin;Kim, Hugh I.
    • Mass Spectrometry Letters
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    • v.7 no.1
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    • pp.16-20
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    • 2016
  • Characterization of intact protein structures in the gas phase using electrospray ionization combined with ion mobility mass spectrometry has become an important tool of research. However, the biophysical properties that govern the structures of protein ions in the gas phase remain to be understood. Here, we investigated the impact of host-guest complexation of ubiquitin (Ubq) with macrocyclic host molecules, cucurbit[n]urils (CB[n]s, n = 6, 7), on its structure in the gas phase. We found that CB[n] complexation induces the formation of compact Ubq ions. Both CB[6] and CB[7] exhibited similar effects despite differences in their binding properties in solution. In addition, CB[n] attachment prevented Ubq from unfolding by collisional activation. Based on the experimental results, we suggest that CB[n]s prevent unfolding of Ubq during transfer to the gas phase to promote the formation of compact protein ions. Furthermore, interaction with positively charged residues per se is suggested to be the most important factor for the host-guest complexation effect.

Incidence, and Identification of Three Root-Knot Nematode species Occurring in the Medicinal Herbs (약용식물의 뿌리혹선충 발생과 분류동정)

  • Park, So-Deuk;Kahn, Zakaullah;Kim, Jae-Cheol;Choi, Boo-Sull;Kim, Tak
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.603-605
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    • 1998
  • Soil and root samples were collected form the rhizoshpere of 11 different medicinal plants to determine the incidence, density and identification of root-knot nematode species associated with medicinal herbs. About 55% of medicinal herbs examined was found to be infested with root-knot nematodes. As a result of infection casued by three root-knot nematodes, M. hapla recorded 43.3% in medicinal herba whereas M. incognita and M. arenaria showed 7.9% and 3.7%, repectively. Forsythia koreana, Hemerocalis fulva, Hibuscus mutabilis and Petasites japonicus were the most severely infested herbs whereas Acanthopanax sessilflorus was least infested. Population of the second stage younger plants. Meloidogyne hapla, M. incognita and M. arenaria were the species associated with the medicinal herbs. The most abundant nematode observed in medicinal herbs was M. hapla and followed by M. incognita and M. arenaria. M. arenaria was observed firstly on Ficus carica, one of medicinal plant.

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Effect of Sulphur on the Yield and Some Quality of Chinese Cabbage (유황시용(硫黃施用)이 배추 품질(品質)과 수량(收量)에 미치는 영향(影響))

  • Lee, Suk-Hee;Kim, Change-Bae;Park, No-Kwuan;Park, Seon-Do;Choi, Boo-Sul
    • Korean Journal of Soil Science and Fertilizer
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    • v.26 no.4
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    • pp.253-258
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    • 1993
  • This study reports the influence of application of sulphur on the yield and some quality by Chinese cabbage in field experiment. 1. By the application of sulphur fertilizer, the yields of Chinese cabbage were increased about 11~13 percent compared to control. 2. After harvest, the available sulphur amount in soil was increased, while the soil pH decreased. 3. The accumulation of nitrate in Chinese cabbage leaves was reduced by the sulphur application. 4. The amount of biochemical materials such as water-soluble pectin, phenolic compound, sugar, vitamin C in leaves were increased by sulphur application.

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Effect of Polypropylene Film Package and Storage Temperature on the Shelf-life Extension of Spinach (Spinacia oleracea L.) (시금치 포장 및 보관온도가 품질보존에 미치는 영향)

  • Choi, Dong-Jin;Lee, Suk-Hee;Yoon, Jae-Tak;Sim, Yong-Gu;Oh, Seok-Gui;Jun, Ha-Joon
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.247-251
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    • 2007
  • To extend the shelf-life of spinach after harvest, we investigated the effect of various packaging methods and storage temperature. In case of polypropylene film package, there was no weight loss, but in non-package, remarkably weight loss occurred as storage period extended and storage temperature risen. Content of vitamin C was decreased rapidly at early stage of storage, and decrease of vitamin C in low temperature storage was lower than that of room temperature storage, but its large difference according to packaging method was not observed. In hunter's value on spinach, b value in room temperature storage was higher than that of low temperature storage. In terms of freshness, shelf-life of spinach by low temperature storage $(1{\sim}3^{\circ}C)$ after PP film packaging lasted 34 days, and that by room temperature storage $(10{\sim}15^{\circ}C)$ after PP film packaging lasted 8 days, but that by room temperature storage after non-packaging lasted 3 days.

Disease Severity of Tobacco Plants Surveyed in the Northern Kyeongbuk Province in 1992 (1992년도 경북북부지역의 담배병해 발생상황)

  • 이영근
    • Journal of the Korean Society of Tobacco Science
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    • v.15 no.1
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    • pp.15-25
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    • 1993
  • The severities of major tobacco diseases had been surveyed throughout northern Kyeongbuk, a major flue-cured tobacco growing district in Korea, in relation to the actual control method used by the farmers in 1992. At seedling stage, anthracnose caused by Colletotrichum nicotianae was major disease of the plant due to poor damage of the temporary transplanting pots. Mosaic caused by tobacco mosaic virus(TMV) was very severe in certain of greenhouses. Although viral disease caused by TMV, cucumber mosaic virus(CMV) and by potato virus Y(PVY) were severe in fields, but mosaic by TMV was major of the diseases. Potato virus Y was found later than that in Honam district. The late occurrence of the disease might be caused the difference between cultivating method of potato plants in northern Kyeongbuk and that in Honam district. In fields suffered from hail disaster, the damage of tobacco plants by angular leaf spot was severe. Most of tobacco growers had wrong information about the practical methods not only of milk treatment for the protection of the plants from TMV, but also of fungicide applications for the control of other major diseases. It suggested that education on the methods to the farmers must be very important for control of the diseases.

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Effect of Packaging and Storage Temperature on the Shelf-life Extension of Kidney Bean (Phaseolus vulgaris L.) (풋꼬투리 강낭콩 보관온도가 품질에 미치는 영향)

  • Choi, Dong-Jin;Cheung, Jong-Do;Sim, Yong-Gu;Choi, Kyung-Bae;Yoon, Jae-Tak;Jun, Ha-Joon
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.96-100
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    • 2008
  • To extend the shelf-life of kidney bean (Phaseolus vulgaris L.) after harvest, we investigated the effect of packaging material and storage temperature. In case of film package, there was no weight loss during storage, but in paper-box package, remarkable weight loss occurred as storage period extended and storage temperature risen. Firmness of kidney bean was greater in low temperature than ambient temperature storage. Content of vitamin C was decreased rapidly during storage, and in paper package, low temperature storage group had lower decrease of vitamin C than that of room temperature. Total chlorophyll content was not difference among storage temperatures in film package treatments. However, in paper-box package, decrease of chlorophyll in room temperature storage was greater than that of low temperature ($8{\sim}10^{\circ}C$). In terms of freshness of kidney bean, we suggest that shelf-life by low temperature storage ($8{\sim}10^{\circ}C$) after PP film or paper package was 8 days, but that by room temperature storage after paper package was 4 days.

Disease Occurrence on Tobacco Plants Surveyed in the Northern Kyeongbuk Area from 1993 to 1994 (1993~1994년도 경북북부지역 담배병해)

  • 이영근;황의홍
    • Korean Journal Plant Pathology
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    • v.10 no.4
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    • pp.292-296
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    • 1994
  • Main tobacco diseases were surveyed on tobacco plants in the northern Kyeongbuk area to evaluate the effectiveness of current disease control techniques from 1993 to 1994. The major disease in seedlings was the anthracnose (Colletotrichum tabacum) but the mosaic (TMV) in adult plants. Wild fire (Psedomonas syringae pv. tabaci) and hollow stalk (Erwinia carotovora subsp. carotovora) were more severe in 1993 than those in 1994 because of more rain. Establishment of drain-outlet at the bottom of the temporary transplanting pot gave a significant reduction in anthracnose severity. Most farmers have ignored the protection effect of using milk during transplanting and the removal of diseased roots from the fields on mosaic (TMV). It was considered that the educating program on disease diagnosis and fungicide application might help farmers for the proper use of chemicals.

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Weathering Properties of Shale Aggregate in Daegu-Kyeongbuk region and Freezing-Thawing Characteristics of Concrete in response to Usage of Shale Aggregate (대경권 셰일 골재의 풍화특성 및 셰일 골재 사용량에 따른 콘크리트의 동결융해 특성)

  • Lee, Seung-Han;Jung, Yong-Wook;Yeo, In-Dong;Choi, Jong-Oh
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.8
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    • pp.4033-4038
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    • 2013
  • Sedimentary rocks from construction waste are discarded through open storage and landfilling, which causes an increase in construction cost and inefficient of execution of works. Some sandstone are selected and utilized as aggregates, but shale is buried as industrial waste. Therefore, in this research, we evaluated weathering properties of shale aggregate that is widely distributed throughout Daegu-Kyeongbuk region and freeze-thaw characteristics of concrete according to the replacement ratio of shale aggregate, in an effort to stabilize aggregate supply-demand in Daegu-Kyeongbuk region and develop alternative aggregates. We used red shale and black shale in the experiment, which were exported from a construction site in Deagu. We verified the usage of shale as a concrete aggregate by comparing andesite, which is broadly used as a thick aggregate for concrete, to hornfels, which is a metamorphic sedimentary rock. As a result of the experiment, we observed no degradation phenomenon for andesite and hornfels. However, a part of country rock containing black shale was found to be exfoliated. Red shale started having cracks in the direction of stratification after 1.5 months of direct exposure, and it broke into smaller pieces after approximately 4 months. After 300 cycles of freeze-thaw process on the concrete manufactured according to the replacement ratio of shale aggregate, the modulus of elasticity was 97% for plain and 95% for hornfels. In the case of RS_100, it was 57% after 210 cycles, and for BS_100, it was 54% after 240 cycles. Therefore, we established that, as the number of repetition increases, the freeze-thaw resistance decreases dramatically.