• Korean Journal of Ichthyology
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• v.10 no.2
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• pp.184-190
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• 1998

The early life history of black bullhead, Pseudobagrus koreanus, endemic to Korea was investigated to get biological information needed in artificial production of seedlings and in recovering natural resources. The fertilized eggs showed some characteristics in having heavy sticky material and minute folds which is formed radical pattern on the egg membrane. The shape of egg was spherical and $2.59{\pm}0.08$(2.45~2.70, n=10)mm in diameter. The yolk had not oil globule. The first cleavage was observed 2 hrs after insemination at $21{\sim}23^{\circ}C$, and the progressive cleavage were done about 30 min. interval. The characteristic changing of the yolk surface started at morula stage and continued to the end of gastrula. Hatching was started 72 hrs and completed 90 hrs after fertilization. The size of the larvae were 5.41~5.81mm in total length and 2.76~2.94mm in preanal length, and the number of so mites was 15-16+33~34(48~50). The barbels and swimbladder were completed and all the fins except second dorsal were appeared 1 week after hatching. The larvae attained 9.67~10.52mm in total length and 5.20~5.65mm in preanal length. All the fin sets and color pattern were completed 2 weeks after hatching and body mucus was secreted at that stage. The juvenile attained 14.59~16.02mm in standard length, 3.31~4.16mm in head length and 8.07~9.31mm in prenal length.

• Environmental and Resource Economics Review
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• v.13 no.3
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• pp.337-366
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• 2004

Contingent Valuation Method (CVM), as non-market valuation approach, has been criticized on that respondents may not realistically reflect their budget constraints in answering willingness to pay (WTP) for hypothetical CV questions. This paper empirically estimates the income calibration factor associated with CV responses through combining travel cost method and contingent valuation method in a utility-theoretic framework. The joint model of recreation demand function and contingent WTP function was applied to an important case study on the Man Kyoung River system, whose water quality is at issue because of the Sae Alan Kum reclamation project. Relevant economic variables such as price, income and water quality had significant influence as anticipated by the economic theory. Equally important, the income calibration factor was not significantly different from one, suggesting that the systematic discrepancies of CV responses relative to the actual behavior was not detected at least in terms of budget exaggeration. Overall, this study supports the notion that carefully designed CVM studies can provide informative data on individuals' willingness to pay for environmental quality changes.

• Korean Journal of Environmental Biology
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• v.19 no.1
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• pp.59-69
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• 2001

Among the biodegradability tests, TOC-HANDAI and OECD method were utilized to examine the degree of biodegradation of endocrine disruptors, Bisphenol A (BPA) and Nonylphenol. Both methods used natural water microcosms and measured their biodegrada-bilities of BPA and Nonylphenol, in terms of TOC or DOC degradation with time for 28 days. Biodegradabilities for BPA, 73-78% with TOC-HANDAI method and 77-81% with OECD method were obtained respectively at the end of experiment. There was no difference in BPA degradation between two methods. BPA degradation was described by two distinct first order decay rates (k$_1$ and k$_2$) which could be separated by a simple visual fitting. Most of the initial decay reaction accelerated within 1-7 days with k$_1$of 0.24-0.34 $day^{-1}$. And the following another long term first order decay coefficient (k$_2$) showed 0.02-0.05 day$^{-1}$ with much flat slope. About 20-25% of initial BPA remained at the end of experiment. It suggests that the remaining TOC components in BPA biodegradation considered to be refractory metabolites of BPA. Nonylphenol at each sampling point was appeared to be mineralized 20-48% of initial TOC concentration. Consequently Nonylphenol seems more recalcitrant against biodegradation. BPA was not detected in the detection limit of ppb in the watershed of Daechung reservoir and Kum river. However 25 ppb concentration of BPA was detected at the influent of industrial wastewater treatment plant in Taejon.

• Journal of Environmental Impact Assessment
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• v.8 no.1
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• pp.107-120
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• 1999

This study was performed to survey the general feature of reservoir sediment in Daecheong, Sapkyo and Kumkang reservoir. For investigations, sediments were sampled at four sites in Dae-Cheong and two sites in SapKyo and Kumkang in June and October. The items for investigation are as follows; water content, loss on ignition(IG), porosity of sediment, contents of element such as hydrogen, nitrogen, carbon, phosphorus contents and nutrient release rates. Loss on ignition was measured to determine the contents of organic substance. And its ranges were determined 2.4~16.2% in Daecheong reservoir, 5.6~27.9% in Sapkyo and 4.8~18.7% in Kumkang reservoir. And, total phosphorus contents in sediments were measured $677{\sim}5,238{\mu}g/g$ in Daecheong, $780{\sim}1,417{\mu}g/g$ in Sapkyo and $604{\sim}1,452{\mu}g/g$ in Kumkang reservoir. And release rates of nutrients were calculated $0.05{\sim}8.63mgP/m^2day$ and $4.99{\sim}36.56mgN/m^2day$ in Daecheong, $1.83{\times}10^{-3}{\sim}3.23{\times}10^{-2}mgP/m^2day$ and $1.97{\sim}.22mgN/m^2day$ in Sapkyo, $8.31{\times}10^{-3}{\sim}6.51{\times}10^{-2}mgP/m^2day$ and $0.89{\sim}4.42mgN/m^2day$ in Kumkang reservoir, respectively. And this study attempted to determine the humus level of sediments. As a result of elemental analysis, C/N ratio was determined 3.0~13.1 in Daecheong reservoir, 6.5~13.0 in Sapkyo and 3.6~12.6 in Kumkang reservoir, respectively. From the elemental analysis, humus levels of reservoirs were changed from mesohumic to oligohumic state in all reservoirs.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.239-244
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• 1997

To develope more effective extraction methods for paraquat in soil, some modification methods were accomplished in two different types of soil. For extraction of tightly bound-paraquat, conc. HCl 70ml were added with different shaking times, and then $H_2SO_4$ reflux were performed for an hour. In this case, 60 minutes shaking were optimum and recovery were increased more $1.09{\sim}1.50$ folds(84.0% in high clay contents soil, but 96.7% in low clay contents soil) and the long-time consuming step, filtration were easily done, with decreasing filtration time were shorter 4.6 folds(ca. $11{\sim}14min.$). than general paraquat analytical method(ca. $55{\sim}65min.$). And only $H_2O_2$ digestion with different volume and refluxing time resulted in recovery increasing. Nevertheless, considering analyst's safety, 30ml of $H_2O_2$ addition and 30 minutes reflux were regarded as optimum condition. Although, Kjeldahl digestion with $H_2O_2$ showed relatively high recovery, it is not significant statistically. For extraction of loosely bound-paraquat, 0.01, 0.1, 1.0, 10.0M of $NH_4Cl$ and of $CaCl_2$ compared with $1.5{\sim}24hr$ of different shaking time. There were no loosely bound residues of paraquat.

• Korean Journal of Agricultural Science
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• v.22 no.2
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• pp.134-142
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• 1995

In order to elevate the efficiency of methane fermentation using the paper mill sludge, this experiment was conducted at two temperature conditions($35^{\circ}C$ and $60^{\circ}C$), and overlooked the addition effects of ethyl acetate as a substrate, nickel as a constituent of $F_430$, and sulfur as a cell growth factor and reductant. The cellulose of paper mill sludge was degraded to lower molecular materials by heating at $60^{\circ}C$ and NaOH treatment. Methane forming rates were 4.8% from NaOH-treated paper mill sludge added with ethyl acetate, 16.5% with sodium sulfide, 19.8% with nickel trioxide, 31.9% with mixture, and 9.6% with control at $60^{\circ}C$, but 0.21% with ethyl acetate, 2.14% with nickel acetate, 3.02% with nickel sulfate, 3.34% with nickel trioxide and 0.62% with control at $35^{\circ}C$. Therefore, methane yield was increased by approximately 10-fold at $60^{\circ}C$ than $35^{\circ}C$, and fermentation liquid added with mixture(nickel trioxide+ethyl acetate+sodium sulfide) at $60^{\circ}C$ showed the medium pH(7.0), higher COD value and lower nitrogen content.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.361-366
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• 1997

To determine the physiological, biochemical characteristics and toxicity of hemolysin produced by a novel sucrose positive Vibrio (Vibrio sp. D5) isolated from estuary of Kum river, it was compared with already known sucrose positive Vibrio. Salinity, pH, temperature and conductivity of place where Vibrio sp. D5 was isolated were $4.7\%_{\circ},\;7.6,\;24^{\circ}C$ and $7800{\mu}MHOS$, respectively. Physiological and biochemical characteristics distingiushed Vibrio sp. D5 from other sucrose positive Vibrio: V. alginoipicus, V. cholerae, V. cincinnatiensis, V. fluvialis, V. furnissii and V. metschnikovii. The range of salinity and pH for growth of Vibrio sp. D5 were $0.5\%\~7.5\%$ and $4.5\~9.5$, respectively. Vibrio sp. D5 exhibited typical yellow colony on TCBS agar plate and curved rod type upon transmission electron microscopy (TEM). Vibrio sp. D5 had lethal toxicity against mouse in case of intraperitoneal injection with its culture and showed hemolysin activity on human blood agar and sheep blood agar. Ubrio sp. D5 also demonstrated vascular permeability activity toward rat. From the above results, Vibrio sp. D5 was ascertained to be a novel pathogenic Vibrio.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.545-550
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• 2002

A hemolysin producing bacterial strain which belong to Vibrio species was isolated from the Kum River estuary. In the process of identification, the strain did not show characteristics of known Vibrio species; thus, the strain was designated as Vibrio sp, E10 (V. kunsan) tentatively and further identification study was carried out by comparing its bacteriological characteristics. Morphologically Vibrio sp, E10 was comma shaped rod with a polar flagellium. Clear hemolysis zones were observed with the strain against human and sheep blood agar. Hemollytic toxicity was confirmed by strong vascular Permeability and fatal toxicity against mouse was also observed. Therefore the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. E10 were ranged salinity of 0$\~$$4.5\%$, pH of 6.2$\~$9.2, temperature of 14$\~$42$^{\circ}C$, respectively, 16S rDNA partial sequence of Vibrio sp, E10 showed $99\%$ homology with dozens of V. cholerae species including V, cholerae El Tor N16961 and V, snmisnfus ATCC 33653T. This strain belonged to Proteobacteria; gamma subdivision; Vibrionacea: Vibrio. But, among knorn Vibrio species no identical styains were found when using automatic bacteria identification system ($MicroLog^{TM}$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp, E10 showed 18 and 11 different responses as compared to V. mimicus and V, cholerae, respectively.

• Journal of Korea Water Resources Association
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• v.46 no.5
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• pp.531-545
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• 2013

This paper was considered on the applicability of EFDC KUNSAN_SEDTRAN MODEL (2012) to calculate Gunsan Port sediment deposition height efficiently and to use for grasping its aspects quantitatively and providing its prevention measures reasonably based on well-known 3-dimensional EFDC sediment transport module. This model was calibrated and verified with various measured field data of A Report of Hydrological Variation on Kum River Estuary (2004). Due to the model calibration and relevant literature investigation for cohesive sediment parameters, settling velocity (WS), critical deposition stress (TD), reference surface erosion rate (RSE), critical erosion stress (TE) were identified as 2.2E-04m/s, 0.20 $N/m^2$, 0.003 $g/s{\cdot}m^2$, 0.40 $N/m^2$ respectivly on this model. In order to examine the applicability and precision of the model computation, the calculated model data of sediment deposition height at 13 stations for 71 days and suspended-sediment concentration at 2 stations, inner port and outer port for 15 days were compared and analyzed with the measured field data. As a result, the model applicability for sediment deposition height simulation was evaluated as NSE coefficient 0.86 and the precision for suspended-sediment concentration computation was evaluated as time averaged relative error (RE) 23%.

• Journal of fish pathology
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• v.8 no.1
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• pp.23-30
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• 1995

We had reported the infection rates of trematode larvae, Cercaria tapidis and C. harengulae in the gonad tissues of Tapes philippinarum and Solen strictus in the estuary of Kum river, the west coast in Korea in 1980~1982. At the same sites from January 1994 to January 1995, we investigated the variation of the infection rates of these trematode larvae in the two clams. The results are follows. The infection rate of C. tapidis parasitized in T. philippinarum increased in 2.4 times (14.0%) higher than that (5.7%) in 1981. Monthly maxium infection rate was 32.0% in October 1994. Comparing the rate (23.3%) in December 1981, the rate in 1994 showed a higher trend than that in 1981. The mean infection rate of C. harengulae in T. philippinarum showed 2.3% in 1994. Comparing that (5%) in 1980, it showed a decreased rate than that in 1980. And monthly maximum infection infection rate of this clam was 6.7% in May 1994, compared with the rate (19.0%) in March 1980. It appeared furthermore decreased rate in 1994. The mean infection rate of the year of C. harengulae in S. strictus was 6.9% in 1994, while it was 10.2% between 1981 and 1982. It showed a decreased trend in 1994. Monthly maximum infection rate was 23.4% in August 1981, while it was 13.3% in April 1994. It showed a decreased trend in 1994 also. In case of C. tapidis the more large sizes of shell length the more infection rates were higher as same in the investigation in 1980, while in C. harengulae the smaller sizes, the more infection rates showed a higher trend.