• Title/Summary/Keyword: Ku80

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Arthrobacter sp. Strain KU001 Isolated from a Thai Soil Degrades Atrazine in the Presence of Inorganic Nitrogen Sources

  • Sajjaphan, Kannika;Heepngoen, Pimpak;Sadowsky, Michael J.;Boonkerd, Nantakorn
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.602-608
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    • 2010
  • An atrazine-degrading bacterium, strain KU001, was obtained from a sugarcane field at the Cane and Sugar Research and Development Center at the Kasetsart University, Kamphaeng Saen Campus, Thailand. Strain KU001 had a rod-to-coccus morphological cycle during growth. Biolog carbon source analysis indicated that the isolated bacterium was Arthrobacter histidinolovorans. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain KU001 was 99% identical to the same region in Arthrobacter sp. The atrazine degradation pathway in strain KU001 consisted of the catabolic genes trzN, atzB, and atzC. Strain KU001 was able to use atrazine as a sole nitrogen source for growth, and surprisingly, atrazine degradation was not inhibited in cells grown on ammonium, nitrate, or urea, as compared with cells cultivated on growth-limiting nitrogen sources. During the atrazine degradation process, the supplementation of nitrate completely inhibited atrazine degradation activity in strain KU001, whereas ammonium and urea had no effect on atrazine degradation activity. The addition of strain KU001 to sterile or nonsterile soils resulted in the disappearance of atrazine at a rate that was 4- to 5-fold more than that achieved by the indigenous microbial community. The addition of citrate to soils resulted in enhanced atrazine degradation, where 80% of atrazine disappeared within one day following nutrient supplementation.

Pervaporation Separation of Water-iso-Propyl Mixture Using PVA/PAA Membranes (PVA/PAA막을 이용한 물-이소프로필알코올 혼합물의 투과증발 분리)

  • Rhim, Ji-Won;Kim, Sun-Woo;Lee, Kew-Ho
    • Membrane Journal
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    • v.6 no.4
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    • pp.284-288
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    • 1996
  • The crosslinked PVA/PAA membranes developed in our laboratory have been characterized for water-isopropyl alcohol mixture in terms of permeabilities and separation factor. When the feed mixture was 12wt% water, the permeability and the separation factor for PVA/PAA=75/25 membrane show $63g/m^{2}h$ and 1520 at $80^{\circ}C$, respectively. In case of 5wt% water in feed mixture, the permeability for PVA/PAA=75/25 membrane is $56g/m^{2}h$ at 8$0^{\circ}C$ while the separation factor is 1563.

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A study on 12W SSPA for earth station transmonder at ku-band (Ku-band 지구국 중계기를 위한 12W SSPA에 관한 연구)

  • 조창환;여인혁;홍의석
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.6
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    • pp.72-80
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    • 1996
  • This paper presetns the development of a SSPA operating at KU-band(14~14.5 GHz) in order of replace TWTA used in the terrestria transponder of a satellite communication. The driving stage of SSPA uses internally matched 2W, 4W, 8W FET and the power stage is coupled with two internally matched 8W FET by branch-line cominer. The SSPA is fabricated with oth the RF circuit and the bias circuitry operating temperature compensation, regulation and sequence on aluminum housing. The SSPA testing resutls implemented in this way show 24.8$\pm$1dB small-signal gain, 41dBm P1dB power, a typical two tone C/IM3, -33dBc with single carrier backed off 6dB from p1dB, and gain stability over temeprature (-30~50)$\pm$1dB.

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A Study on the Design of VCO Using Junction Capacitance of Active Element (능동소자의 접합 커패시턴스를 이용한 VCO 설계에 관한 연구)

  • Kang, Suk-Youb;Park, Wook-Ki;Go, Min-Ho;Park, Hyo-Dal
    • Journal of Advanced Navigation Technology
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    • v.8 no.1
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    • pp.57-65
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    • 2004
  • In this paper, keeping pace with light weight, pocket-size, lower-price, we design VCO(Voltage Controlled Oscillator) X/Ku band for using at public RD(Radar Detector) to apply to controlled voltage on base in transistor which used as a oscillator, without using varactor diode in part of VCO tuner. As a result of simulation, we conclude VCO could be have 110 MHz by controlled voltage 4.25 V to 4.80 V and show its output 9.63 dBm at operating frequency, 11.46 GHz, and its phase noise -107.2 dBc at 1 MHz offset frequency. So it turned out suitable performance for commercial use.

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Implementation of a Waveguide Cross Guide directional Coupler for Ku-Band using Polynomial Approximations for the Polarizabilities of the Rounded End Slot (끝이 둥근 슬롯의 분극률에 대한 다항 근사식을 이용한 Ku-Band용 도파관 십자형 방향성 결합기 제작)

  • 유경완;이재현;박광량;김재명
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.31A no.8
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    • pp.7-15
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    • 1994
  • In this paper, the directional coupler for Ku-band, which is designed is of the crossguide type with a coupling value of about 3-dB. The apertures chosen for this design are crosses. We used polynomial approximations of rounded end slot to obtain the electric and magnetic polarizability of crossed-slot and compared the results with Cohn's experimental results. The optimized dimensions and positions of the cross aperture are obtained by a trial-and-error reiteration of the program. This paper presents the measurement results for the designed coupler. The very flat coupling shows a total variation of only 29.80$\pm$0.04dB for the design frequency ranges of 12.25GHz-12.75GHz. The measured minimum directivity is 25dB. The aperture attenuation for the finite diaphragm thickness is about 2dB pr 0.5mm, which is in agreement with the theoretical value. The transmission loss and input return loss at center frequency are 0.0564dB and 48.16dB respectively. We obtained the measured minimum directivity of the coupler. Whose apertures are both circles is better than that containing holes in the performance of directivity.

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Quality of Mozzarella Cheese Analogues Prepared from Soy Milk with WP, WPC 34, WPC 80, DWP, or LP during the Storage Period (다양한 유청제품인 WP, WPC 34, WPC 80, DWP, LP를 Soymilk에 첨가하여 제조된 Mozzarella Cheese Analogue의 저장 중 품질 변화에 관한 연구)

  • Jin, Woo-Seung;Song, Kwang-Young;Seo, Kun-Ho;Yoon, Yoh-Chang
    • Journal of Dairy Science and Biotechnology
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    • v.31 no.1
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    • pp.35-49
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    • 2013
  • The purpose of this study was to develop Mozzarella cheese analogues by using dairy products in the form of WPC 34, WPC 80, whey protein, demineralized whey powder, and lactose powder along with soy milk. Soy milk was separately blended with 5% WPC 34 (A), WPC 80 (B), DWP (C), WP (D), and LP (E) and also with 10% WPC 34 (F), WPC 80 (G), DWP (H), WP (I), and LP (J). Blending of soy milk and whey products showed that increase in the proportions of whey products (WPC 34, WPC 80, DWP, WP, and LP) led to increase in the protein, lactose, and SNF levels of the admixture. A decrease in fat content was observed for all cheeses prepared from mixtures, relative to those for the control cheese. The nitrogen content within analogue samples was higher than that in the control cheese and increased with increase in the proportions of whey products within soy milk. Higher water soluble nitrogen levels were observed in cheese prepared from whey-product-blended soy milk than in the control cheese. The non-protein nitrogen level within the control Mozzarella cheese was significantly lower than that in the Mozzarella analogues, and, in the case of cheese analogues, it increased with increase in the proportion of whey products in soy milk. With regard to the physicochemical and sensory qualities of the Mozzarella cheese analogues and control cheese, the pH of all analogue samples, with the exception of the cheese prepared from group G, was lower than that of the control Mozzarella cheese. Rheological studies showed that the hardness of Mozzarella cheese analogues was lower than that of the control Mozzarella, while the elasticity, cohesiveness, and brittleness of the analogues was higher. The control sample had a higher meltability level than any of the Mozzarella analogues. Mozzarella cheese prepared with the traditional method had higher browning and stretching levels than all the cheese analogues, but a lower oiling-off level.

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Studies on the Development of Improved Health Beverages containing Bioactive Peptide from Hydrolysates of Cheese Whey Protein: A Review (생리활성 펩타이드를 함유하는 치즈 유청단백질 가수분해물로부터 기능성 건강음료 개발에 관한 연구: 총설)

  • Yoo, Sung-Ho;Seo, Kun-Ho;Chon, Jung-Whan;Kim, Hyun-Sook;Song, Kwang-Young;Lim, Jong-Soo;Yoon, Sung-Sik;Paik, Hyun-Dong;Yoon, Yoh-Chang
    • Journal of Dairy Science and Biotechnology
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    • v.31 no.2
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    • pp.109-125
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    • 2013
  • Recently, functional foods and bioactive components in foods have drawn the attention and interest of food scientists, nutritionists, health professionals, and general consumers. Bioactive whey protein is a highly concentrated milk serum isolate or concentrate, which is high in protein (80~90% protein by weight), carbohydrate- and sugar-free, and nonfat or very low in fat. Bioactive whey protein enhances both healthy and deficient immune systems. In general, ultrafiltered whey protein contains various whey protein concentrate peptides, which could be used for manufacturing probiotics added to health beverages. Hence, the objective of this paper was to review the published literature on research of new functionally improved health beverages using various bioactive components extracted from milk and dairy products.

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Spheroplast Formation, Regeneration and Fusion of Flavimonas oryzihabitans KU21 (Flavimonas oryzihabitans KU21의 원형질체 생성, 재생 및 융합)

  • 이수연;임영복;박용근;이영록
    • Korean Journal of Microbiology
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    • v.31 no.4
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    • pp.318-325
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    • 1993
  • The optima] conditions for the formation, the regeneration. and the spheroplast fusion of Flavimonas aryz/habitans spheroplasts were investigated. Cells were transformed to spherop]asts effectively by treatment of 0.5% volume (v/v) of 0.] M EDTA and ]00 flg/ml lysozyme at $37^{\circ}C$ for 30 min without shaking. Magnesium chloride and calcium chloride were effective on the stabilization of spheroplasts. and 20 mM calcium chloride in the rich regeneration medium improve the yield of regenerants as much as 3.5-fo]d. Addition of 0.8% bovine serium albumine (BSA) in dilution buffer for spheroplast formation improved the stabilization of spheroplasts over extended periods (4-6 hr) at room temperature. and thus increased the yield of recombinants to 4.5-fold. The spheroplast formation frequency and regeneration frequency of F aryzihabitans strain was 90.10% and 3.800/." respectively. The first regenerated cell of F. aryzihabitans spheroplasts were appeared 6 hours after plating. By I I hours after plating, 80% of spheroplasts were regenerated on thc rich regeneration medium containing 0.5 M sucrose. The intraspeci11c spheroplast fusion of F urvz/habitans was carried out and the properties of obtained fusants were investigated. Formation of fusion products was effective when the Flav/munas spheroplast mixture was treated with 40%(w/v) PEG6000 and 20 mM CaCl, for 10 min at room temperature. and thc formation of frequency of recombinants were $2.0{\times}10^{-5}~3.6{\times}10^{-5}$. All tested recombinant clones were very stable on further propagation.

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