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Evaluation of the Fermentation Ability of Lactic Acid Bacteria to Manufacture Highest Quality Rice Straw Silage (고품질 볏짚 사일리지 제조를 위한 다양한 유산균주 평가)

  • Lee, In-Sok;Lee, Song-Yee;Choi, Min-Kyung;Kang, Chan-Ho;Kim, Jeong-Man
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.106-111
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    • 2018
  • The main objective of this study was to determine the quality of rice straw silage made with various lactic acid bacteria (LAB) during fermentation. Five strains of LAB (Lactobacillus plantarum CMRT, L. leuconostoc mesenteroides M17, L. sakei C11, M5, SP2) were used in this study. With regard to odor, ready-made CMRT (a comparison strain) had the highest value from 10-60 days, followed by M17. The pH level of all silages made with five strains (apart from CMRT) ranged from 4.02 to 4.59. Of these, M17 rapidly lowered the pH value in the silage. Crude protein (CP) content was significantly lower (p < 0.01) in ready-made CMRT compared to the other bacteria. C11 fermentation produced the highest content of silage, with a score of 5.56. Acetic acid and butyric acid were not detected in any of the silage products. The lactic acid levels in silages produced by M17, M5, C11, and SP2 were comparatively higher than that produced by CMRT. The total digestible nutrient (TDN) content levels and relative fees value (RFV) were he highest in the silage of C11 (69.65 and 155.56, respectively), followed by M17. Based on odorl, pH, protein, organic acid, and feed value, we suggest tha the M17 strain is a suitable substitute for CMRT that can be used to generate high quality rice straw silage.

The Effect of Algae on Coagulation and Filteration of Water Treatment Process (정수처리과정중 응집및 여과에 미치는 조류의 영향)

  • Lim, Young-Sung;Song, Won-Seb;Cho, Ju-Sik;Lee, Hong-Jae;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.13-19
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    • 2000
  • Seasonal variations of dominant algae species and the effects of these algae on coagulation and filteration of water treatment were investigated at Chilseo water treatment plant in downstream of Nakdong river from January in 1995 to Desember of 1998. The water quality of Nakdong river was found to be a hyper eutrophic state during the investigation periods. In the measurement, Chlorophyll-a contents ranged $20.7{\sim}180.9{\mu}g/l$ and total nitrogen contents(T-N) and total phosphorus contents(T-P) exceeded more than 3.4mg/l and 0.1mg/l, respectively. The changes in dominant algae species was in the order of Stepanodiscus sp., Asterionella sp., Melosira sp., Microcystis sp. and Synedra sp. from spring to winter. Microcystis sp. especially, was blooming during summer and Synedra sp. and Stepanodiscus sp. during winter. Although most diatomous algae appeared in the water treatment process caused filter clogging and reduced efficiency of coagulation and sedimentation, Synedra sp. and Stepanodiscus sp were revealed as the main trouble algae. Malfunction of water treatment process caused by Synedra sp. and Stepanodiscus sp. started at the algae concentrations of 800cells/ml and 1,820cells/ml, respectively. When chlorophyll-a content was $18.9{\mu}g/l$, the optimum amounts of coagulant were found to be 40mg/l of Alum and 16mg/l of PACS. Under condition of chlorophyll-a content of $154.1{\mu}g/l$, addition of Alum at the level of 75mg/l and PACS at the level of 35mg/l showed the lowest turibidity. The result indicates that increased amounts of the coagulants should be added for a better water treatment as chlorophyll-a contents increased. Addition of Alum at the amount of 60mg/l and 30mg/l of PACS removed Stepanodiscus sp. algae at the rate of 85% and 83%, respectively. In case of Synedra sp., 50mg/l of Alum and 25mg/l of PACS showed removal rates of 79% and 81%, respectively. Synedra sp. algae at the standing crops of 1,500cells/ml started filter clogging and a filtering process was completely inhibited after 8 hours. At this situation the filter clogging by Synedra sp. algae occurred at the depth of 5cm from the top anthracite layer. On the other, other algae did filter clogging at the depth of 10cm.

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Screening of Nutrient Removal Hydrophyte and Distribution Properties of Vegetation in Tributaries of the West Nakdong River (서낙동강 유역 하천의 식생 분포특성과 영양염류 정화 수생식물 탐색)

  • Kim, Choon-Song;Ko, Jee-Yeon;Lee, Jae-Saeng;Hwang, Jae-Bok;Park, Sung-Tae;Kang, Hang-Won
    • Korean Journal of Environmental Agriculture
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    • v.25 no.2
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    • pp.147-156
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    • 2006
  • This study was conducted to investigate natural distribution of aquatic plane and to find out natural aquatic plants which highly absorb nutrient N and P. We surveyed vegetation within ${\pm}2m$ from streamside in 12 tributaries of the West Nakdong river watershed during May to October in 2003. Hydrophytes surveyed in tributaries of the West Nakdong river watershed were 27 families, 61 genera, 76 species, 3 varieties. Major dominance species of aquatic plants were Z. latifolia, P. communis, P. thunbergii, P. arundinacea, P. japonica, and P. distichum var. indutum. Aquatic plants having high production ability of biomass were Z. latifolia, P. communis, P. arundinacea, P. japonica, and E. crus-galli var. echinata. In the vertical distribution of hydrophytes within streams, dominant species were P. thunbergii and P. japonica in the upper stream, but dominant species in the downstream were P. communis and Z. latifolia. Species diversity or aquatic, plants was reduced, but their biomass and nutrient (T-N and T-P) content per the natural area unit $(m^2)$ were increased in the downsteaam. Nutrient N and P content of aquatic plants per the natural area unit were high at Joman river, Pyeonggangcheon, Bulam drainage canal, and Hogyecheon. Fifty-seven species of aquatic plants having high biomass were grounped into 4 categories $(I{\sim}IV)$ according to their nutrient content per dry weight unit. I group $(T-N,\;\geqq20gkg^{-1}\;&\;P_2O_5,\;\geqq7gkg^{-1})$ was comprised of 3 submerged plants (H. verticillata, P. crispus, and C. demersum), e emergent plants (O. javanica, P. distichum var. indutum, and R. sceleratus), 1 suspended plant (T. japonica), and 1 riparian plant (A. lobatum). Otherwise, in classification of natural hydrophytes according to their nutrient content per natural area unit, Z. latifolia, P. communis, P. longiseta, P. arundinacea, and P. distichum var. indutum possessing great biomass productivity as emergent plants were included in I group $(T-N,\;\geqq1gm^{-2}\;&\;P_2O_5,\;\geqq0.7gm^{-2})$.

Estimation of Agricultural Water Quality Using Classification Maps of Water Chemical components in Seonakdong River Watershed (수질성분 분포도를 이용한 서낙동강 수계 농업용수 수질평가)

  • Ko, Jee-Yeon;Lee, Jae-Sang;Kim, Choon-Song;Jeong, Ki-Yeol;Choi, Young-Dae;Yun, Eul-Soo;Park, Seong-Tae;Kang, Hwang-Won;Kim, Bok-Jin
    • Korean Journal of Environmental Agriculture
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    • v.25 no.2
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    • pp.138-146
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    • 2006
  • To understand the status of water quality and work out a suitable countermeasures in Seonakdong watershed which has poor agro- environmental condition because of severe point and non-point source pollution by popularized city and near sea, we investigated the pollution sources and water quality from '03 and '05 and the result were mapped with GIS and RS for end-users's convenient comprehense and conjunction of water quality and geological data. The most degraded tributary was Hogeo stream which was affected directly by highly popularized Gimhae city, the main pollution source of the watershed. The pollution of tributaries in watershed increased the T-N of main body that reached over 4 mg/L during dry season. Pyeonggang stream and the lower part of main water way were suffered from high salt contents induced near sea and the EC value of those area were increased to 2.25 dS/m. The delivered loads of T-N and T-P were largest in Joman river as 56% and 61% of total delivered loads 1mm tributaries because of lots of stream flow. When Management mandate for irrigation water in Seonakdong river watershed was mapped for estimating integrated water quality as the basis of classification of EC and T-N contents in water, Hogeo and Shineo catchments were showed the requiring countermeasures none against nutrients hazard and Pyeonggang catchment was the vulnerable zone against nutrients and salts hazard. As the result, Seonakdong watershed had very various status of water quality by characteristics of catchments and countermeasures for improving water quality and crop productivity safely should changed depend on that.

Comparison of Nutrient Balance in a Reclaimed Tidal Upland between Chemical and Compost Fertilization for the Winter Green Barley Cultivation (간척농경지에서 비종에 따른 동계 청보리 재배 포장의 영양물질 수지 비교)

  • Song, In-Hong;Lee, Kyong-Do;Kim, Ji-Hye;Kang, Moon-Seong;Jang, Jeong-Ryeol
    • Korean Journal of Environmental Agriculture
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    • v.31 no.2
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    • pp.137-145
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    • 2012
  • BACKGROUND: Along with the surplus rice production, introduction of upland crop cultivations into newly reclaimed tidal areas has gained public attentions in terms of farming diversification and farmers income increase. However, its impacts on the surroundings have not been well studied yet, especially associated with nutrient balance from reclaimed upland cultivation. The objective of this study was to investigate water and nutrient balance during winter barley cultivation as affected different fertilization methods. METHODS AND RESULTS: TN and TP balance for three different plots treated by livestock compost, chemical fertilizer, and no application were monitored during winter green barley cultivation (2010-2011) at the NICS Kyehwa experimental field in Jeonbuk, Korea. Nutrient content in soil and pore water near soil surface appeared to increase, while sub-soil layer remained similar with no fertilization plot. Livestock compost application appeared to increase organic matter content in surface soil compared to chemical fertilization. Crop yield was the greatest with livestock compost application (10.6 t/ha) followed by chemical fertilization (6.9 t/ha) and no application (1.8 t/ha). The nitrogen uptake rate was also greater with livestock compost (52.4%) than chemical fertilizer (48.1%). Phosphorus uptake rate was much smaller (about 7.0%) compared to nitrogen. Nutrient loss by surface and subsurface runoff seemed to be minimal primarily due to small rainfall amount during the winter season. Most of the remaining nutrients, particularly phosphate seemed to be stored in soil layer. Phosphate accumulation appeared to be more phenomenal in the plot applied by livestock compost with higher phosphorus content. CONCLUSION: This study demonstrated that livestock compost application to tidal upland may increase barley crop production and also improve soil fertility by supplying organic content. However, excessive phosphorus supply with livestock compost seems likely to cause a phosphate accumulation problem, unless the nitrogen-based fertilization practice is adjusted.

Residual Characteristics and Monitoring of Cyenopyrafen and Cyflumetofen in Strawberries for Export (수출딸기 중 Cyenopyrafen과 Cyflumetofen의 잔류소실 특성평가 및 잔류농약 모니터링)

  • Kim, Yeong-Jin;Kim, Jong-Hwan;Kwon, Young-Sang;Song, Jong-Wook;Seo, Jong-Su
    • Korean Journal of Environmental Agriculture
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    • v.36 no.4
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    • pp.279-287
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    • 2017
  • BACKGROUND: Many farmers who cultivate the strawberries for export have used agricultural chemicals which MRL (Maximum Residue Limits) of main export target countries or simultaneous multi-residue analysis in Korea have not been established. Among them, the cyenopyrafen and cyflumetofen were selected and applied to this study to determine the PHI (pre-harvest interval) which is appropriate to the PLS (Positive List System) criterion (0.01 mg/kg) and to investigate the residual amounts in the samples. In addition, Fifty pesticides were monitored to check up whether it is suitable or not for main export target countries. METHODS AND RESULTS: Cyenopyrafen and cyflumetofen were spayed out to the strawberries. Samples for residual analyses were taken for maximum 60 days. After sampling, they were extracted by the QuEChERS method and analyzed using the LC-MS/MS. Cyenopyrafen and cyflumetofen were detected in a range of 0.0106~2.6517 mg/kg and of 0.0005~1.4480 mg/kg, respectively. From this results, they were found to be suitable for PLS concentration after 30 or 45 days after spray. In addition, they were detected in most samples that were selected at random. Their concentrations were higher than the PLS criterion in the maximum twenty samples. Twelve of pesticides unsuitable for main export target countries have been detected in the monitoring of simultaneous multi-residue analysis. The result indicates they are unsuitable for export since they excesses over PLS criterion. CONCLUSION: The monitoring result showed it is necessary to establish the pesticide standards of safe use suitable for the PLS criterion. In addition, it is considered continues management and inspection are needed to solve problems caused by unsuitable pesticides in export strawberries.

Relationship between Damage by Herbivore and Leaf Production of Oaks in the Burnt Area of the East Coastal Region, Korea (동해안의 산불피해지역에서 참나무 잎 생산량과 초식 피해의 관계)

  • Lee, Kyoung Sin;Hong, Bo Ram;Lee, Kyu Song
    • Korean Journal of Environmental Biology
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    • v.36 no.2
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    • pp.206-216
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    • 2018
  • We analyzed the effects of spatio-temporal variation in the leaf production of oaks on the density and species richness of herbivores, as well as the consumption by herbivores in the east coastal region of Korea, which is an area that has been damaged by forest fires. The main herbivore that feeds on oak leaves was moth larvae. In mid-August the insect larvae showed the highest density and species richness. Approximately 60.5% of total plant-eating insect larvae were present from August to September 2011. Oak leaf production was at its peak from July to August, and the peak damage caused by herbivores was from August to September. Depending on the investigation timing and site of the survey, oak leaf production, larval densities, and species richness showed large variations. The average production of oak leaves between July and August was estimated to be $0.96ton\;ha^{-1}$. The production of oak leaves during this period also showed spatial variations ranging from 0.34 to $1.89ton\;ha^{-1}$. In August, the consumption of oak leaves by the herbivores showed spatial variations ranging from 0.15 to $1.51ton\;ha^{-1}$. Where oak leaves had a higher yield, they tended to increase in density and species richness of the herbivores. As the production of oak leaves increased, so did the overall consumption and consumption rate by the herbivores. This means that the production of oak leaves is highly related to time and space, and there is a concentration response in which the new individuals gather. Research into the spatio-temporal heterogeneity of the food sources and their effects on the higher levels of the food web can help us quantitatively understand and evaluate the structure and functions of the burnt ecosystem that is caused by forest fires.

Establishment of Microbial Criteria by Investigation of Microbial Contamination in Ready-to-Eat Foods (즉석섭취·편의식품류의 미생물 오염도 조사를 통한 기준·규격 재평가)

  • Song, Bo Ra;Kim, Soon Han;Kim, Jin-Kwang;Han, Jeong-A;Kwak, Hyo Sun;Chung, Kyung-Tae;Heo, Eun Jeong
    • Journal of Food Hygiene and Safety
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    • v.32 no.5
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    • pp.348-354
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    • 2017
  • Internationally different food safety regulation and standards could cause troubles in trade unless those are built on scientific knowledge. In this research, we monitored the microbial population and analyzed the results to determine the level of microbial contamination in foodstuffs using relatively new statistical analysis (microbiological sampling plan, International Commission on Microbiological Specification for Foods). The goal of this research falls on establishing entirely new standards for various food categories addressed in the Korean "Food Code". Targets for monitoring were indicator organisms (i.e. total aerobic count, coliform and Escherichia coli) and foodborne pathogens (i.e. Bacillus cereus, Staphylococcus aureus and Clostridium perfringens) in ready-to-eat (RTE) products. As the result of the monitoring, total aerobic count, coliform, E. coli, and B. cereus in RTE products were found at the mean values of 2.10 log CFU/g, -0.60 log CFU/g, -1.33 log CFU/g and -1.23 log CFU/g, respectively. S. aureus was detected with the level of -1.35 log CFU/g only in fresh-cut food, while C. perfringens was -1.37 log CFU/g only in ready-to-cook food. Other samples did not have any food borne pathogens. Total aerobic count, B. cereus, S. aureus and C. perfringens satisfied the Food Code (the MFDS). On the basis of the analysis, we proposed a draft of microbial criteria for RTE products.

Carbon Budget Evaluated in Two Urban Parks of Seoul (서울의 두 도시 근린공원에서 평가된 탄소수지)

  • Kim, Gyung Soon;Pi, Jung Hun;An, Ji Hong;Lim, Chi Hong;Jung, Song Hie;Joo, Seung Jin;Lee, Chang Seok
    • Korean Journal of Ecology and Environment
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    • v.49 no.1
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    • pp.51-61
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    • 2016
  • This study was carried out to assess the carbon budget of two urban parks and one natural park and to prepare the plan for improving the ecological functions of the park including carbon sink. Net Ecosystem Production (NEP) of those study sites was calculated from the relationship between Net Primary Production (NPP) and soil respiration of each study site. To understand carbon budget of the whole area designated as the park, carbon budget of the urban park was analyzed by classifying the vegetated and the non-vegetated zones. NEP of the Nohae and the Sanggye parks calculated by reflecting areal size that the non-vegetated zones occupy were shown in -1.0 and $0.6\;ton\;C\;ha^{-1}yr^{-1}$, respectively. On the other hand, NEP of Mt. Bulam natural park as a reference site was in $2.3\;ton\;C\;ha^{-1}yr^{-1}$. Based on the result, the Nohae park was assessed as carbon source rather than carbon sink. On the other hand, the Sanggye park was classified as carbon sink but the role was poor compared with natural park. The result is, first of all, due to lower NPP of the vegetation introduced for the parks compared with natural vegetation. The other reason is due to wide arrangement of non-vegetated zone. To solve those problems and thereby to create the urban park with high ecological quality, selection of plant species suitable for the ecological characteristic of the park, their arrangement imitated natural vegetation, and ecological zoning were recommended.

Analysis of Correlation with Cross Sectional Area of Flow and Flow Rate Variation of Discharge Measurement Point in the Upper Stream of Seomjin River (섬진강상류 유량측정지점의 유수단면적과 유량변화에 따른 상관관계 분석)

  • Song, KwangDuck;Kim, KapSoon;Lee, DongJin;Ham, SangIn;Kim, DaeYoung;Oh, TaeYoun;Lee, JaeChoon;Lim, ByungJin
    • Korean Journal of Ecology and Environment
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    • v.46 no.1
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    • pp.94-102
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    • 2013
  • This study was carried out to determine the variation of the water level and crosssection area for investigating changes of stream foreland, and to determine the correlation between the average flow velocity and cross-section area so as to understand the hydrological characteristics of the stream. The slope of the cross-sectional area was changed in water levels of 0.6~1.0 m and 1.8~2.0 m. The first change occurred in the low-water level season, and the second change occurred in the high-water level seasons. It is assumed that the changes occurred due to the geological transfigure. The correlation between the cross-sectional area and the average flow velocity was 0.22~0.86 in the exponential equation and 0.20~0.87 in the linear equation. The low water level had a higher correlation than the high water level, and free weirs in the upper stream showed a very low correlation. Therefore, this study provides novel information for the management of water quality in the riverside, using correlation equations of the water level and flow velocity with the cross section area.