• Korean Journal of Ichthyology
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• v.21 no.3
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• pp.208-213
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• 2009

Histological examination of an individual of the Korean slender gudgeon Squalidus gracilis majimae having cutaneous albinism produced rarely in the wild was made and compared with a normal individual. The external body of the albino was colorless, differing from the normal individual, which has dense brownish black spots over its body surface. To make it clear through histological study, we observed eight skin regions: dorsal, lateral, ventral, upper caudal peduncle, lower caudal peduncle, dorsal fin, anal fin, and the eyes. These regional skins were the same in fundamental structure between albinic and normal gudgeon, but there were significant differences in distribution and development of pigment cells (melanins). In the normal gudgeon, the pigment cells were well developed over the regional skins except on the skin from the ventral region. However, it was confirmed in the albino that the pigment cells were vestigial over the upper regions of the eye and body but absent in the ventral region, lower caudal peduncle, and anal fin.

• Korean Journal of Ecology and Environment
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• v.38 no.2 s.112
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• pp.207-216
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• 2005

Spawning behaviors of Squalidus gracilis majimae (Cyprinidae) were observed in the laboratory whose environmental factors such as light (D/L = 16 : 8), temperature ($20\;{\sim}\;24\;＾{\circ}C$), and dissolved oxygen (>8 mg $L^{-1}$) were quite regularly controlled. The behavioral patterns were categorized into three stages ofpre-spawning, spawning, and Post-spawning behaviors. In Particular, the pre-spawning stage was specified by 11 specific behavioral patterns of aggressive mating behaviors. During the spawning stage, the male and female performed four distinct spawning behaviors including sexual temptation, stimulation, egg spawning, and the separation, and randomly laid about 200 ${\sim}$ 300 eggs on the bottom substrates through the night. After finishing spawning, two adults separated toward their refuges and showed 3 types of post-spawning behaviors such as the resting, occasional eggs protecting, and the egg eating. The fish was identified as a partial-parental care species after the spawning.

• Korean Journal of Ecology and Environment
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• v.38 no.1 s.110
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• pp.73-82
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• 2005

This study was conducted, based on the field survey and laboratory observations, to elucidate egg developmental processes and their characteristics of the Korean slender gudgeon, Squalidus gracilis majimae. For the experiments, the mature adults were collected at the Woongcheon-Cheon Stream and Boreung Reservoir located in Boreung City, Chungnam Province and eggs were obtained from the natural spawning area. Morphological characteristics of the egg and embryonic development were summarized as follows: The shape of the fertilized egg was spherical, adhesive and transparent. The fertilized egg was 2.9${\pm}$0.3 mm (n = 30) in mean diameter under water temperature of $26{\pm}1.5^{\circ}C$, light white in color and had no oil droplets. After 20 minutes from the time of fertilization, a blastodisc was formed and divided into two cells at 48 minutes after fertilization. The blastular stage occurred at 5 hours 40 minutes after fertilization and the gastrular stage was detected at 8 hours 41 minutes after fertilization. The beginning of embryo formation was observed at 12 hours 58 minutes after fertilization and optic vesicles and 9 somites were discovered at 17 hours 05 minutes after fertilization. Differentiation of brains and embryo wiggling were observed at 37 hours 27 minutes after fertilization. Heart beating and the formation of melanophores in optic vesicles were detected at 44 hours 46 minutes after fertilization. The formation of pectoral fins and melanophores in the body were discovered at 50 hours 36 minutes after fertilization. Hatching occurred at 57 hours 49 minutes after fertilization. The newly hatched larvae were 3.3${\pm}$0.2 mm (n = 120) in total length. We believe that these results may contribute the species and population conservations under the situation of accelerated water pollution and the decreases of its diversity.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.114-120
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• 1997

Frequencies and the types of abnormal vertebrae in the juvenile of five cyprinid fishes collected from the Kumho River during 1995 and 1996 were examined. Types of vertebral anomalies in investigated species were spinal curvature, fused vertebrae, helical sutures of cetera and abnormal vertebrae with one or two additional spines. The frequencies and the types of vertebral anomalies were different among the species. Of all the examined species, the type with one or two additional spines showed the highest frequencies, 11.72~12.11%. The frequencies of fused vertebrae was 4.45~7.68%. Thes two types of vertebral anomalies were observed in all species. Among the several types of fused vertebrae, the frequencies of double fused vertebrae were higher than those of other types. Also, the incidence of fused vertebrae located in the caudal region of vertebral column was much higher than that in other regions. The percentages of spinal curvature and helical sutures of vertebrae in the investigated species were 0.02~0.15% and 0.02%, respectively. Among the examined specimens, vertebral anomalies include fused vertebrae and one or two additional spines were shown in the three species, Korean slender gudgeon (Squalidus gracilis majimae), False dace (Pseudorasbora parva) and Crucian carp (Carassius auratus). In addition to the two vertebral anomalies, spinal curvature was shown in the Korean gudgeon (Squalidus chankaensis tsuchigae). Dark chub (Zacco temmincki) had fused vertebrae, one or two additional spines, spinal curvature, and helical sutures of vertebrae. This species has the most variable vertebral anomalies. Frequencies of fused vertebrae and one or two additional spines in the all tested fishes were not related with their standard lengths measured. However, spinal curvature and helical sutures of vertebrae were shown only in the specimens smaller than 20mm in standard length.

• Applied Microscopy
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• v.28 no.1
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• pp.63-71
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• 1998

The spermatozoon of Squalidus gracilis majimae is approximately $36.6{\mu}m$ in length and is characterized by a spherical nucleils with the clear chromatin, a short midpiece containing the mitochondria, and a flagellum positioned tangentially to the nucleus. An acrosome is absent as in all teleost fishes. The nucleus is about $1.9{\mu}m$ in diameter and in its periphery contains the electron-lucent chromatin distinguished from the electron-dense chromatin occupying most of the nucleus. The shallow nuclear fossa contains the proximal centriole, instead of two centrioles in deep nuclear fossa in siluroids. The proximal and distal centrioles are oriented approximately $140^{\circ}$ to each other. The mitochondria of 10 or more in number are arranged in three layers and do not surround the axoneme. The asymmetrical distribution of the mitochondria and the eccentrical position of the nucleus with regard to the tail are the general pattern of the cyprinid spermatozoa. S. gracilis majimae spermatozoa have the most mitochondria and the deepest cytoplasmic canal among cyprinid species. The flagellum lacks the lateral fins.

• Korean Journal of Environment and Ecology
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• v.35 no.3
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• pp.277-284
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• 2021

This study surveyed the ecological characteristics of Squalidus gracilis majimae at Nakdong River tributary Sinan Stream from January to December 2020. The species inhabited the riverbed that was mostly covered with gravel and sand. The water depth was 27-158 cm, and the average was rather deep at 54 cm. The stream velocity was slow at 0.41±0.19 (0.24-0.86) m/sec. The gender ratio of females to males was 1:0.73. According to the total length-frequency distribution, the age indicated that the group with less than 50 mm in total length was one year old, the group with 70-69 mm was two years old, and the group over 70 mm was three years old. The total length of sexually mature fishes was 50 mm for females and 60 mm for males. The spawning season was from June to July, and the water temperature was 20.9-23.4℃ during the period. The prosperous spawning season was July. The average number of eggs in the ovaries was 1,009 (595-1,630) per matured female, and the matured eggs were yellowish and spherical with a mean diameter of 0.78±0.08 (0.57-0.83) mm. The live foods were S. gracilis majimae Protozoa, attached algae, zooplankton, and aquatic insects. The feeding habits of S. gracilis majimae are omnivorous, but more than 80% of the stomach content was aquatic insects, Chironomidae spp. The larger the size of this species, the greater the amount and frequency of eating aquatic insects, which are animal feeds. The population that ate aquatic insects did not eat vegetable food.

• Korean Journal of Ecology and Environment
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• v.42 no.2
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• pp.260-269
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• 2009

Hwabuk Dam has been under construction to reduce flood damage in Nakdong River watershed and to supply stable water for middle area of Gyeongbuk Province. Therefore, fish investigation in up and downstream of the dam was conducted from 2004 to 2008 in order to determine any negative effect on fish community due to dam construction and to use as fundamental data for conserving species diversity and maintaining stream health. According to data analysis on water quality, temperature, dissolved oxygen, pH, suspended solids, and total E-coli had seasonal variation, but they did not significantly differ in sites. However, biological and chemical oxygen demand, chlorophyll-a, nitrogen, and phosphorus representing organic matter and nutrient concentration were higher in upper site and decreased to lower site so that they differed by site. Concentration of arsenic among the heavy metals was less than 0.05 mg $L^{-1}$, which is regulated for protection of human health in water quality standard, except for 0.092 mg $L^{-1}$ in June 2005. During the study period, the total number of fish caught from the 6 sites was 10,263 representing 7 families 19 species. Among them, dominant and subdominant species were Korean chub (Zacco koreanus, 62.5%) and Chinese minnow (Rhynchocypris oxycephalus, 10.6%) which inhabit mostly in mid and upper streams, Korea. Among the 19 species, Korean endemic species were 9 species (47.4%) including Korean slender gudgeon (Squalidus gracilis majimae), Korean dark sleeper (Odontobutis platycephala), and Korean shiner (Coreoleuciscus splendidus). There was several individuals of the $1^{st}$-class endangered species, Naktong nose loach (Koreocobitis nahtongensis), caught in 2005${\sim}$2007, and no introduced species of fish was found in entire sampling period. According to result of community analysis, dominance index decreased toward lower site, but diversity and richness indices increased toward lower site. The equation of length-weight relationship on the dominant species was TW=0.000003$(TL)^{3.2603}$. The parameter b in the equation was greater than 3.0 indicating good nutritional condition in the populations. Compared to populations of Korean chub in other streams, the population in Hwabuk Dam watershed had higher mean of condition factor by size indicating better growth rate. With fish fauna and multi-metric health assessment model in each sampling attempt, index of biotic integrity (IBI) was evaluated and it resulted mostly in good (26${\sim}$35) and excellent (36${\sim}$40) condition in all sites, and the mean of IBI was the highest in site 5. The results indicate that it is very important to study not only environmental impact assessment with fish composition but also stream health assessment in order to conserve healthy aquatic ecosystem.