• 한국식품영양과학회지
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• 제30권1호
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• pp.112-118
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• 2001

To study effect of non-fat components present in plant seeds on lipid metabolism, defatted safflower and perilla seed powders were used in high cholesterol diets for ovariectomized (ovx) female Sprague-Dawley rats weighing 227$\pm$15g. Experimental groups were six as follows; normal group without ovariectomy and cholesterol-free diet, sham and ovx-control groups with high cholesterol and cellulose for dietary fiber, ovx-est group with the same diet as ovx-control but with eight subcutaneous injections of 50$\mu\textrm{g}$ 17$\beta$-estradiol. ovx-safflower and ovx-perilla with 29% and 16% (w/w) of each defatted powder in high cholesterol diets at the expense of cellulose. Weight gains were lower in normal and sham groups and food efficiencies were lower in normal,ovx-est and ovx-safflower groups compared with ovx-control. Uterus weights were dramatically reduced by ovariectomy but restored completely by 17$\beta$-estradiol and partially (~5%) by defatted safflower. Plasma levels of total cholesterol were not different among ovx-control, sham, vx-est and ovx-safflower groups (90.8~95.1 mg/dL) but that was lower in ovx-perilla (80.4$\pm$6.2 mg/dL). Plasma triglyceride (TG) levels were lower in sham (76.6$\pm$7.0 mg/dL) and ovx-perilla (79.2$\pm$5.8 mg/dL) groups. Liver cholesterol levels were lower in sham, ovx-est, ovx-safflower and ovx-perilla groups (26.6~29.8 mg/g) than ovx-control (36.5$\pm$3.2 mg/g). But liver TG levels were reduced only sham and ovx-est groups compared to control group. Fecal excretions of bile acid and cholesterol were highest in ovx-safflower group (30.8$\pm$5. and 32.1$\pm$5.7 mg/g) compared with other ovx groups (20.8~23.1 and 12.1~19.5 mg/g). These results suggest that both perilla and safflower seeds contain groups (20.8~23.1 and 12.1~19.5mg/g). These results suggest that both perilla and safflower seeds contain non-fat and non-fiber components having lipid lowering effects.

• 한국식품저장유통학회지
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• 제9권3호
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• pp.309-314
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• 2002

홍화씨의 식품재료로서의 이용성을 증가시키기 위한 목적으로서 양갱의 주원료인 건조 팥소 분말에 대한 볶은 홍화씨 분말 20, 35, 45 및 60mesh별로 첨가한 제품과 홍화씨 45mesh분말의 첨가량을 0, 5, 10, 15 및 20%로 하여 제조한 제품 및 배합수로 녹차추출물을 이용한 제품의 품질특성을 조사하였다. 수분함량은 20mesh 체분리 분말 첨가 제품에서 가장 낮은 27.6% 이었고, 동일한 45mesh 분말의 첨가량에 따른 양갱제품의 수분함량은 분말의 첨가량이 증가할수록 증가하는 경향을 보였다. 수분활성도는 60mesh 홍화씨 분말 첨가 제품에서 0.93로 가장 높았고, 45mesh분말의 첨가량에 따른 수분활성도는 분말의 첨가량이 증가할수록 높아지는 경향이었다. 색도는 분말의 mesh 수가 증가할수록 L값과 a값이 다소 낮아지는 경향이었고 반대로 b값은 다소 증가하였다. 동일한 입자크기를 가진 분말의 첨가량 증가에 따른 L, a 및 b값은 전반적인 증가현상을 보였다. 또한, 조직감 특성 중 견고성과 부숴짐성은 홍화씨 분말의 입도가 미세한 첨가구가 상대적으로 증가하였고 동일한 입도를 가진 홍화씨 분말 첨가량이 증가함에 따라 견고성과 부숴짐성은 증가하였고, 접착성과 탄력성은 감소하는 경향을 나타내었다. 관능평가에서도 전반적기호도, 조직감 및 부숴짐성이 홍화씨 분말의 입도가 미세한 첨가구가 상대적으로 증가하는 결과를 보였고, 증류수를 배합수로 제조한 제품과 증류수와 녹차추출물을 1:1로 혼합하여 제조한 제품에서 거의 같은 수준의 높은 관능점수를 얻었다.

• 한국식품과학회지
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• 제36권2호
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• pp.196-201
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• 2004

식품재료 및 약리 효과로 인한 약용재료로서 사용범위가 확대되고 있는 홍화꽃 및 홍화씨의 휘발성 성분을 분석 동정하였다. 그 결과, 홍화꽃의 주요 휘발성 성분으로는 테르펜류인 p-cymene, limonene, ${\alpha}-phellandrene$, ${\gamma}-terpinene$, camphor, 4-terpineol, selinene, ${\beta}-caryophyllene$, torreyol, ${\beta}-eudesmol$ 등이 검출되었다. 또한, phenylacetaldehyde, 2-phenylethylalcohol, 4-terpineol, ${\beta}-caryophyllene$, ${\gamma}-terpinene$, ${\alpha}-phellandrene$ 등 통상의 꽃향기 성분과 알데히드류인 3-methylbutanal, 2-methylbutanal, pentanal, hexanal, octanal, nonanal 및 decanal, 알코올류인 2-pentanol 및 heptanol, 케톤류의 3-penten-2-one, 2-heptanone, 2,6,6-trimethyl cyclohexanone 등이 함유되어 있었다. 그 이외 산류인 acetic acid, heptanoic acid, 2-methylbutanoic acid 및 rancid-cheese odor를 띄는 3-methylbutanoic acid(isovaleric acid) 등의 생성이 확인되었다. 특히, 홍화꽃에서의 이들 산류의 생성은 다른 꽃류에 비해 종류나 함량이 많았으며, 홍화꽃이 다른 꽃에 비해 좋지 않은 냄새를 내는 특징적인 요인으로 생각되었다. 한편, 홍화씨의 경우는 hexanal, (E)-2-heptenal, (E,Z)-2,4-decadienal 및 (E,E)-2,4-decadienal등의 알데히드류가 주요 휘발성 성분으로 동정되었다. 일반성분 중 전체 함량의 약 30%의 지질을 함유하는 홍화씨는, 방향성과 관계된 torreyol, ${\beta}-eudesmol$ 등의 terpene alcohol류, 달콤한 향과 관계가 있는 3-methylbutanal 및 2-methylbutanal과 함께 산류의 생성도 있었으나, 지방산 산화 분해생성물인 (E,Z)-2,4-decadienal 및 (E,E)-2,4-decadienal이 전체 휘발성 함량의 약 75%를 차지하였다.

• Food Science and Biotechnology
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• 제16권3호
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• pp.392-397
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• 2007

We investigated whether ethanol extracts of the safflower seeds containing phenolic compounds were responsible for the bone-protecting effects. Crude ethanol extract (CEE) of the safflower seeds was fed for 4 weeks at the level of 1% in diet to female Sprague-Dawley rats that had been subjected to bilateral ovariectomy (OVX). The CEE effects (OVX+CEE) were evaluated by comparing results obtained from OVX, Sham, and OVX injected with $17{\beta}$-estradiol ($OVX+E_2$) groups. OVX resulted in a dramatic reduction in the trabecular bone mass of the proximal tibia (approximately 40% of the Sham group) and an increase in fat deposition in bone marrow. In $OVX+E_2$ group, the bone loss was completely prevented as well as marrow adiposity. In OVX+CEE group, approximately 80% of the bone mass was maintained compared with Sham group and fat deposition in the bone marrow was prevented. Meanwhile, the partially purified ethanol extract containing the phenolic compounds stimulated proliferation of the ROS 17/2.8 osteoblast-like cells in a dose-dependent manner, as potently as positive controls of $E_2$ and genistein. The present data demonstrate that the ethanol extracts of safflower seeds reduced bone loss caused by estrogen deficiency. The bone-protecting effect of safflower seeds seems to be mediated, at least partly, by the stimulating effect of the phenolic compounds on the growth of osteoblasts.

• Nutrition Research and Practice
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• 제5권1호
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• pp.20-27
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• 2011

We conducted this study to examine the effects of safflower seed granular tea containing physiologically active polyphenols on antioxidative activities and bone metabolism. Forty postmenopausal women ages 49 to 64-years were recruited from Daegu and Gyeongbuk and were randomly assigned to either a safflower tea supplement (Saf-tea) group (n=27) or a placebo group (n=13). The Saf-tea group received 20 g of safflower seed granule tea per day containing a 13% ethanol extract of defatted safflower seeds, whereas the placebo group received a similar type of tea that lacked the ethanol extract. No significant changes in nutrient intake for either the placebo or Saf-tea groups were observed before or after the study period, except vitamin A intake increased after 6 months in the Saf-tea group. Dietary phytoestrogen intakes were similar in the Saf-tea group (60.3 mg) and placebo group (52.5 mg). Significant increases in plasma genistein and enterolactone were observed in the Saf-tea group. After 6 months of supplementation, serum levels of antioxidant vitamins such as a-tocopherol and ascorbic acid increased significantly, and TBARS levels decreased in the Saf-tea group compared to the placebo group. Serum osteocalcin levels were reduced (P<0.05) in the Saf-tea group after 6 months, whereas serum osteocalcin did not change in the placebo group. Urinary deoxypyridinoline/creatinine excretion was not different between the two groups at baseline, and did not change in either group after 6 months. Bone mineral density decreased significantly in the placebo group (P<0.01) but not in the supplemented group. It was concluded that polyphenols (72 mg/day), including serotonin derivatives, in the Saf-tea had both antioxidant and potential bone protecting effects in postmenopausal women without liver toxicity.

• 한국약용작물학회지
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• 제8권3호
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• pp.194-200
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• 2000

홍화종실의 볶음처리에 따른 성분의 변화를 조사하고 볶음처리 후 저장기간에 따른 산패의 정도를 측정한 결과 홍화씨의 볶음처리에 따른 수율의 변화에서 볶음온도 및 시간이 증가 할수록 수율이 감소하였다. 홍화의 볶음온도 및 시간에 따른 색도의 변화에서 볶음온도가 높고 볶음시간이 길수록 명도(L)값은 감소하고 적색도(a) 값과 황색도(b) 값은 증가하였으며 전체적인 색차인 ${\Delta}E$ 값은 증가하였다. 관능평가의 결과에서는 홍화종실의 적정 볶음조건은 $190^{\circ}C$ 20분 이었다. 홍화종실에서는 raffinose와 sucrose만이 분리 확인되었다. 이들 유리당은 볶음시간이 길어짐에 따라 그 함량은 급격히 감소하였다. 홍화종실의 산패 정도를 나타내는 과산화 물가 (POV, meq/kg)를 측정 해 본 결과 볶음처리직후에는 거의 과산화물이 생성되지 않았지만 저장기간이 지남에 따라 과산화물값이 높아짐을 보였다. 특히 $150^{\circ}C$를 제외하고는 10개월 이상 저장하는 것은 부적절하였다.

• 한국식품저장유통학회지
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• 제16권5호
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• pp.726-733
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• 2009

본 연구에서는 6종류의 종실에서 기름을 착유하여 조지방 함량(crude fat content) 측정 및 총 지방산 조성과 위치별 지방산 조성을 비교 분석 하였으며, triacylglycerol(TAG)의 조성과 tocopherol 함량을 분석하였다. Folch법을 이용하여 분석된 조지방 함량은 들깨 21.64%, 홍화씨 13.85%, 고추씨 9.60%, 석류씨 8.85%, 녹두 2.25%, 결명자 2.00%로 나타났다. 추출된 종실유의 지방산 분석결과 불포화 지방산인 linoleic acid(C18:2)가 81.57 wt%~46.17 wt%로 가장 높은 비중을 차지하였으며, sn-2 위치의 지방산 조성에서도 linoleic acid의 함량이 88.30 wt%~15.99 wt%로 높은 함량을 나타내었다. Triacylglycerol(TAG)의 조성은 reversed-phase HPLC를 이용하여 분석하였으며 partition number(PN)=36~48의 분포를 보였다. Total tocopherol의 함량은 석류씨에서 가장 높은 377.74 mg /100 g으로 나타났으며 이 외에 각각 녹두(141.16 mg/100 g), 결명자(107.23 mg/100 g), 고추씨(33.88 mg/100 g), 들깨(30.05 mg/100 g), 홍화씨(29.80 mg/100 g) 순으로 측정되었다.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2003년도 춘계총회 및 제22차 학술발표회
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• pp.22-39
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• 2003

Biological activity of phenolic compounds in seeds and leaves of safflower (Carthamu tinctorius L.) were evaluated using several in vitro and in vivo assays. Six phenolic constituents were isolated from the seeds and identified as N-feruloylserotonia, N- (p-coumaroyl)serotonin, matairesinol, 8′-hydroxyarctigenin, acacetin 7-O-$\beta$-D-glucoside (tilianine) and acacetin. Six phenolic compounds exhibited considerable antioxidative activity, and especially two serotonins showed potent DPPH radical scavenging activity and antiperoxidative activity against rat liver microsomal lipid peroxidation induced by the hydroxyl radical generated via a Fenton-type reaction. Additionally, six phenolic compounds possessed comparable cytotoxicity against three cancer cells, Hela cell, MCF-7 and HepG2 cell, and particularly acacetin and its glycosides had the most potent cytotoxicity. Moreover, we found that feeding safflower seeds attenuated bone loss, and lowered levels of plasma and liver lipids in ovariectomized rats. Serotonins, lignans and flavones stimulated proliferation of the osteoblast-like cells in a dose-dependent manner (10$^{-15}$ ~10$^{-6}$ M), as potently as E$_2$ (17$\beta$-estradiol). Particularly, serotonins were mainly responsible for bone-protecting and lipid lowering effects in ovariectomized rats. Meanwhile, eight flavonoids, including a novel quercetin-7-O-(6"-O-acetyl)-$\beta$-D-glucopyranoside and seven kown flavonoids, luteolin quercetin, luteolin 7-O-$\beta$-D-glucopyranoside, luteolin-7-O-(6"-O-acetyl)-$\beta$-D-gluco-pyranoside, quercetin 7-O- -glucopyranoside, acacetin 7-O-$\beta$-D-glucuronide and apigenin-6-C-$\beta$-D-glucopyranosyl-8-C-$\beta$-D-glucopyranoside were first isolated and identified from safflower leaf. Among these flavonoids, luteolin-acetyl-glucoside and $\beta$quercetin- acetyl-glucoside showed potent antioxidative activities against 2-deoxyribose degradation and lipid peroxidation in rat liver microsomes. Luteolin, quercetin and their corresponding glycosides also exhibited strong antioxidative activity, while acacetin glucuronide and apigenin-6, 8-di-C-glucoside were relatively less active. Finally, changes in phenolic compositions were also determined by HPLC in the safflower seed and leaf during growth stages and roasting process to produce standardized supplement powerds. These results suggest that phenolic compounds in the roasted safflower seed and leaf may be useful as potential sources of therapeutic agents against several pathological disorders such as carcinogenesis, atherosclerosis and osteoporosis.

• 한국작물학회지
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• 제49권6호
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• pp.503-506
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• 2004

홍화 재배시 비효면에서 효과적인 황 시용방법을 구명하고자 황분말 20 kg/10a와 유안의 시용방법 및 시용량을 달리하여 생육 및 수량에 미치는 영향을 비교 조사한 결과는 다음과 같다. 1. 황 유형 및 시용 정도에 따른 생육은 황 시용시 무시용에 비하여 초장이 큰 경향이었으며, 경태와 경엽중이 굵거나 무거웠고, 황 유형간에는 황분말 보다는 유안 처리시 양호한 경향이었다. 2. 황을 시용함으로써 화두수 등 수량구성요소가 전반적으로 증가하는 경향을 보였고, 종실 수량이 $4-10\%$ 증수되었다. 황 유형간에는 황분말보다는 유안이 효과적이었으며, 유안 시비방법 간에는 엽면시비 8.3kg/10a과 토양시비 $33.0\~49.5kg/10a$ 시용구와 차이가 미미하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제9권1호
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• pp.55-62
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• 2005

Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, $3{\mu}l$ of 0.1% dried crude extract or $2{\mu}l$ of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells: $3{\mu}l$ of 0.1% dried crude extract and $2{\mu}l$ of 0.1% dried aqueous fraction significantly increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells ($8{\times}10^{-4}$) to the extent that it deserves a considerable attention. Furthermore, the crude extract and aqueous fraction increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells, and $300{\mu}M$ $Cd^{2+}$, specific calcium channel blocker, completely blocked the increase. Therefore, the increased $[Ca^{2+}]_i$ of the cultured osteoblast cells by safflower seed component continued to activate calcium channel.