• Journal of Environmental Health Sciences
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• v.40 no.4
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• pp.313-321
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• 2014

Objectives: Pine needles are used as passive air samplers (PAS) of atmospheric persistent organic pollutants (POPs). This study was carried out in order to investigate whether pine needles can also be used as a PAS of polybrominated diphenyl ethers (PBDEs). Methods: PBDEs in one to three year-old pine needles were analyzed with HRGC/HRMS. Results: PBDEs in the atmosphere were accumulated in the pine needles. The rate of increase in concentration of lower substituted PBDEs (tri- through hepta-) in pine needles was linear. The rate of increase of the higher substituted PBDEs (octa- through deca-) was also liner, but only up to two year-old pine needles. The concentration did not increase further in older pine needles. Conclusion: Therefore, it can be concluded that young pine needles, those that are two years old or less, are recommended for use as PAS of atmospheric PBDEs.

• Culinary science and hospitality research
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• v.9 no.4
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• pp.179-190
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• 2003

This study was performed to investigate the effects of dried powders of pine needle, pine pollen, green tea and horseradish on preservation of Kimchi-yangnyum. The physicochemical and microbial changes of Kimchi-yangnyum were investigated during 30-days preservation. The changes of pH, total acid, and number of total viable cell, lactic acid bacteria, E. coli of the Kimchi-yangnyum were insignificant. And the addition of pine needle powder were more effective to suppress fermentation than that of pine pollen or green tea or horseradish.

• Mycobiology
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• v.35 no.3
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• pp.159-161
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• 2007

Pine tree death caused by pine wood nematode(PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle(JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. Frpm JPS adult beetles, nine genera of Aspergillus, Gibberalla, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidio-mycetes were found. Ten genera from PWN-infected weed were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectria, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.

• Journal of Ecology and Environment
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• v.41 no.9
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• pp.265-270
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• 2017

Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

• The Korean Journal of Food And Nutrition
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• v.26 no.4
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• pp.646-654
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• 2013

This study was conducted to investigate the effect of pine needle powder on antioxidant activity and the quality characteristics of Majakgwa (Korean traditional cookie). Majakgwa was prepared with different amounts of pine needle powder (in ratios of 0, 3, 6, 9 and 12% to the flour quantity). The antioxidant activity was estimated by DPPH free radical scavenging activity and by the total phenol content in pine needle powder and Majakgwa. For analyzing the quality characteristics, bulk density and pH of the dough, moisture content, volume, color, texture profile analysis and sensory evaluations were measured. The bulk density, volume, total phenol contents and DPPH free radical scavenging activity of Majakgwa significantly increased with increasing pine needle powder (p<0.001), whereas the pH of the dough, L values and b values of the Majakgwa significantly decreased with increasing pine needle powder content (p<0.001). The consumer acceptability score for the 6% pine needle Majakgwa ranked significantly higher (p<0.001) than those of the other groups in overall preference, flavor, taste, crispiness and color. Acid value and peroxide value was lower in Majakgwa with pine needle powder than control. From these results;we suggest that pine needle powder is a good ingredient for increasing consumer acceptability and functionality of Majakgwa.

• Journal of the Korean Wood Science and Technology
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• v.50 no.3
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• pp.186-192
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• 2022

This study examined the utility of pine (Pinus densiflora) pollen cones as an environmentally friendly material with sound-absorbing properties. Pine pollen cone samples with widths of 0.8-1.2 cm and lengths of 3.5-4.5 cm were prepared. After filling impedance tubes to heights of 6, 8, 10, or 12 cm with the pine pollen cones, the sound absorption coefficient of the pine pollen cones was investigated. The peak sound absorption frequency of the samples with a thickness of 6 cm was reached at 1,512 Hz; however, this value shifted to 740 Hz in samples with a thickness of 12 cm. Therefore, the sound-absorbing performance of pine pollen cones at low frequencies improved as the material thickness increased. According to KS F 3503 (Korean Standards Association), the sound absorption grade of pine pollen cones ranges from 0.3 to 0.5 M, depending on the material thickness of the pine pollen cones. In conclusion, the pine pollen cones demonstrated good sound absorption properties. They, thus, may be considered an environmentally friendly sound-absorbing material.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.469-475
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• 1993

In order to elucidate the metabolism of the systemic insecticide, phosphamidon(2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate), treated to pine trees against pine leaf gall midges (Thecodiplosis japonensis Uchida et Inouye), $[vinyl,\;carbonyl-^{14}C]$phosphamidon was implanted into the trunks of 10-year-old Korean red pine (Pinus densiflora Sieb. et Zucc.) and Japanese black pine (Pinus thunbergii Parl.), respectively. This chemical was degraded very quickly in pine trees after implanting, as confirmed by TLC/autoradiography of the extracts of pine needles. Phosphamidon metabolites in phosphate buffer extracts of pine needles include the major metabolite, ${\alpha}-chloroacetoacetic$ acid diethyl-amide, ${\alpha}-chloroacetoacetic$ acid ethylamide, 3-hydroxy-N,N-d iethylbutanamide, acetoacetamide, and trimethyl phosphate. The metabolism within pine trees is expected to be similar to this. Based on these findings, it is believed that the major pathway leading to the metabolites would be related to the P-O-vinyl hydrolysis of the chemical structure.

• Korean journal of food and cookery science
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• v.33 no.2
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• pp.174-180
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• 2017

Purpose: The purpose of this study was to investigate quality characteristics and antioxidant effects on storage by temperature between new cultivar Dewdrop Pine Mushroom (Lentinula edodes GNA01) and button mushroom (Agaricus bisporus). Methods: Dewdrop Pine Mushroom and button mushroom were prepared at low storage temperature. Results: Weight loss increased with time, whereas the new cultivar Dewdrop Pine Mushroom showed a decrease of less than 4% until 15days. Hardness of new cultivar Dewdrop Pine Mushroom was reduces less than button mushroom during storage. Color change of new cultivar Dewdrop Pine Mushroom was not altered during storage. For viable cell count, new cultivar Dewdrop Pine Mushroom proliferated less than button mushroom. For antioxidant activity, polyphenol content increased with storage period of both mushrooms. The electron-donating action of new cultivar Dewdrop Pine Mushroom maintained high antioxidant activity accounting for 80% until 12 days of storage. New cultivar Dewdrop Pine Mushroom was evaluated as better than pine mushroom as it exceeded the middle of storage in sensory characteristics; such as appearance, color, aroma and overall acceptability, etc. Conclusion: In summary, new cultivar Dewdrop Pine Mushroom was stored for 12 days while button mushroom was stored for 9 days.

• Journal of Korean Society for Atmospheric Environment
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• v.18 no.6
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• pp.515-525
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• 2002

A field study was conducted to estimate the emission rate of biogenic volatile organic compounds (BVOCs) from pine trees. In addition, the influences of meteological variables on their distribution characteristics have been investigated. A vegetation enclosure chamber was designed and constructed of Tedlar bag and acril. Sorbent tubes made up of Tenax TA and Carbotrap were used to collect biogenic VOCs emitted from each individual tree. Analysis of BVOCs was performed using a GC-FID system. The fundamental analytical parameters including linearity, retention time, recovery efficiency, and breakthrough volume were examined and verified for the determination of monoterpene emission rates. Total average concentration of each component is found to be $\alpha$-pinene (16.5), $\beta$-pinene (4.61) from pine trees, and $\alpha$-pinene (42.4), $\beta$-pinene (18.7 ng(gdw)$^{-1}$ hr$^{-1}$ ) from Korean pine trees. On the basis of our study, $\alpha$-pinene was found to be the major monoterpene emitted from both pine and Korean pine trees which were accompanied by $\beta$-pinene, camphene, and limonene. In ambient air, variable monoterpene compositions of emissions from pine trees were similar to Korean pine trees. Emission rates of monoterpene from each tree were found to depend on such parameters as temperature and solar radiation.

• Journal of the Korean Applied Science and Technology
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• v.21 no.4
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• pp.320-326
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• 2004

We have extracted the natural pine-needles to use ethanol in solvent, and could obtain the refined oil component from pine-needles extract. We have tested the antimicrobial effect from microbe experiment and analyzed with ICP/OES, GC/MS. we could obtain the next conclusion from the result of this experiment. In the first result of this experiment we could know that in case of increasing concentration of pine-needles, the number of microbe decreased more and more. Also we could know that the refined oil component of pine-needles appeared the sterilization effect of S-aureus and E-coli after 96hrs and 120hrs. So we could know that the refined oil component of appeared effect to microbe. In the second result of this experiment we could know that inorganic materials of Ca, Mg, V, Mn etcs from pine-needles detected to ICP/OES analysis and the aromatic compounds from refined oil component of pine-needles was certificated to GC/MS analysis.