• Journal of Aquaculture
• /
• v.20 no.4
• /
• pp.265-269
• /
• 2007

We studied the effects of temperature on the egg development and larvae of Takifugu pardalis. The larvae hatched 135-480 hr after fertilization in $13^{\circ}C-24^{\circ}C$ and 32 ppt. The higher the temperature the earlier the larvae hatch within $13^{\circ}C-24^{\circ}C$. The maximum hatching rate was made in $15^{\circ}C$ and 32 ppt. The higher the temperature the larger the newly hatched larvae. The larvae hatched in $24^{\circ}C$ were ca. 3.35 mm in total length. Occurrence of the deformed larvae was lower in $13^{\circ}C-18^{\circ}C$ regime and higher over $21^{\circ}C$ regime.

• Korean Journal of Ichthyology
• /
• v.19 no.2
• /
• pp.168-172
• /
• 2007

In the present study, attempts were made to find out the physico-chemical properties of milt and the sperm motilities in various osmotic conditions using Panther puffer, Takifugu pardalis. The average concentration of sperm in the milt was $12.1{\pm}3.2{\times}10^9/mL$. pH and osmolality of seminal plasma were $8.2{\pm}0.3$, $385.5{\pm}12.5mOsm/kg$, respectively. Spermatozoa were immotile when the milt was mixed with solutions (electrolyte or non-electrolyte) of lower osmolality than the average seminal plasma osmolality ($385.5{\pm}12.5mOsm/kg$), but became motile after mixing milt with hyperosmotic solutions.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.2
• /
• pp.218-230
• /
• 2020

The single anatomical attribute that has the greatest influence on acoustic scattering from fish is the presence or absence of a swimbladder. This study examined the effect of removing the gas from the swimbladder on the broadband backscattering characteristics of six species of swimbladdered fish: striped beakperch Oplegnathus fasciatus, black scraper Thamnaconus modestus, dark-banded rockfish Sebastes inermis, goldeye rockfish Sebastes thompsoni, black rockfish Sebastes schlegeli and panther puffer Takifugu pardalis. Before and after removing the gas from the swimbladder, the species-specific, frequency-dependent backscattered echo signals from anaesthetized individuals of each fish species were measured at approximately 1° intervals spanning a 90° aspect angle range from -45° (head down) to +45° (head up) using a broadband echo sounder operating at 100-200 kHz. The relationship between the wavelength-normalized backscattering cross section (σ/λ²) and fish length (L/λ) was calculated for each species. The average σ/λ² value for the six fish species at a L/λ range of 19.79-25.85, with a mean of 22.89, was reduced by approximately 52.3% when the gas was removed.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.2
• /
• pp.129-134
• /
• 2014

Two neuropeptides were purified from the acidified tube feet extract of the starfish Asterias amurensis by C18 reversed phase and size-exclusion high-performance liquid chromatography (HPLC). The tube feet extract and the purified peptides (AST-I and AST-II) showed potent contractile activity on dorsal retractor muscle (DRM) of the starfish Asterina pectinifera and intestine (smooth muscle) of the panther puffer Takifugu pardalis. Treatment of the purified peptides with dithiothreitol (DTT) for 60 min at $37^{\circ}C$ significantly altered their retention times, suggesting that these compounds contained disulfide bonds. The molecular weights of AST-I and AST-II were determined to be 2064.2 Da and 6137.2 Da, respectively, by MALDI-TOF mass spectrometry.

• Journal of Life Science
• /
• v.24 no.5
• /
• pp.491-497
• /
• 2014

Nowadays, starfish is one of problems about interruption of marine ecosystem, so many researchers are focusing on application of the starfish (Asterias amurensis). In this study, we investigated the contractile and relaxant activity of the smooth muscles and the antimicrobial and antioxidant activity of six different tissues (muscle, gut, liver, tube feet, gonads, and body) of A. amurensis. Frozen samples were extracted with distilled water containing 1% acetic acid. Extracts from all the tissues, except the body tissue, showed potent antimicrobial activity against Escherichia coli D31. The dorsal retractor muscles (DRM) of muscle and gut extracts showed strong contraction responses. On the other hand, contractile activity on esophagus of squid Todarodes pacificus could be detected in all tissues tested. The contractile activity of the liver extract was higher than that of the other tissues. The body, tube feet, and liver extracts showed the contractile activity on the intestine of the panther puffer fish (Takifugu pardalis). Relaxation response on the DRM of starfish (A. pectinifera) was observed in all tissues tested. Increased antioxidant activity was observed in the gut, liver, and body extracts. The results suggest that the starfish (A. amurensis) is a potential source of novel bioactive compounds.