Browse > Article

Milt Property and Sperm Motility of Panther Puffer, Takifugu pardalis  

Kho, Kang Hee (Faculty of Marine Technology, Chonnam National University)
Publication Information
Korean Journal of Ichthyology / v.19, no.2, 2007 , pp. 168-172 More about this Journal
Abstract
In the present study, attempts were made to find out the physico-chemical properties of milt and the sperm motilities in various osmotic conditions using Panther puffer, Takifugu pardalis. The average concentration of sperm in the milt was $12.1{\pm}3.2{\times}10^9/mL$. pH and osmolality of seminal plasma were $8.2{\pm}0.3$, $385.5{\pm}12.5mOsm/kg$, respectively. Spermatozoa were immotile when the milt was mixed with solutions (electrolyte or non-electrolyte) of lower osmolality than the average seminal plasma osmolality ($385.5{\pm}12.5mOsm/kg$), but became motile after mixing milt with hyperosmotic solutions.
Keywords
Takifugu pardalis; sperm; milt; motility; osmolality;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bouck, G.R. and J. Jacobson. 1976. Estimation of salmonid sperm concentration by microhematocrit technique. Trans. Am. Fish. Soc., 105 : 534-535   DOI
2 De Quatrafages, M.A. 1853. Recherches sur la vitalite des spermatozoides de quelques poissons d,eau douce. Annales des sciences naturelles: Troisieme serie, 19 : 341-369
3 Hogendoorn, H. and M.M. Vismans. 1980. Controlled propagation of the African catfish, Clarias lazera. Aquaculture, 21 : 39-53   DOI   ScienceOn
4 Morisawa, M., K. Suzuki, H. Shimizu, S. Morisawa and K. Yasuda. 1983. Effects of osmolality and potasium on motility of spermatozoa from freshwater cyprinid fishes. J. Exp. Biol. 107 : 95-103
5 Ohta, H. and T. Izawa. 1996. Diluent for cool storage of Japanese eel (Anguilla japonicus) spermatozoa. Aquaculture, 142 : 107-118   DOI   ScienceOn
6 Chang, Y.J. 1997. Present and future studies on the cryopreservation of fish gametes. Suisanzoshoku, 45 : 557-564
7 Kho, K.H. 2001. Studies of the transmembrane cell signaling for the initiation of sperm motility in salmonid fishes. Ph.D. thesis In 'Life Science Institute, Graduate School of Science', University of Tokyo, Tokyo
8 Harvey, B. and R.N. Kelley. 1984. Chilled storage of Sarotherodon Sarotherodon mossambicus milt. Aquaculture, 36 : 85-95   DOI   ScienceOn
9 Kho, K.H., M. Morisawa and K.S. Choi. 2004. The Role of $Ca^{2+}$ and calmodulin on the initiation of sperm motility in Salmonid Fishes. J. Micro. Biotech., 14 : 456-465
10 Chao, N.H., H.P. Tsai and I.C. Liao. 1992. Short- and longterm cryopreservation of sperm and sperm suspension of the grouper, Epinephelus malabaricus (Bloch and Schneider). Asian Fish. Sci., 5 : 103-116
11 De Kruger, J.C., G.L. Smith, J.H.J. Van Vuren and J.T. Ferreira. 1984. Some chemical and physical characteristics of the semen of Cyprinus carpio L. and Oreochromis mossambicus (Peters). J. Fish Biol., 24 : 263-272   DOI
12 Morisawa, M., S. Tanimoto and H. Ohtake. 1992. Characterization and partial purification of sperm-activating substance from egg of the herring, Clupea pallasii. J. Exp. Zool., 264 : 225-230   DOI
13 Hwang, P.C. and D.R. Idler. 1969. A study of major cations, osmotic pressure and pH in seminal components of Atlantic salmon. J. Fish. Res. Bd. Can., 26 : 413-419   DOI
14 Morisawa, M., K. Suzuki, H. Shimizu, S. Morisawa and K. Yasuda. 1983. Effects of osmolality and potassium on motility of spermatozoa from cyprinid fishes. J. Exp. Biol., 107 : 95-103
15 Ohta, H. and M. Tsuji. 1998. Ionic environment necessary for maintenance of potential motility in the common carp spermatozoa during in vitro storage. Fish. Sci., 64 : 547-552   DOI
16 Lahnsteiner, F., B. Berger, T. Wiesmann and R. Patzner. 1996. Changes in morphology, physiology, metabolism, and fertilization capacity of rainbow trout semen following cryopreservation. Prog. Fish. Cult., 58 : 149-159   DOI   ScienceOn
17 Kho, K.H., S. Tanimoto, K. Inaba, Y. Oka and M. Morisawa. 2001. Transmembrane Cell Signaling for the Initiation of Trout Sperm Motility: Roles of ion channels and membrane hyperpolarization for cyclic AMP synthesis. Zoological Science, 18 : 919-928   DOI   ScienceOn
18 Stoss, J. 1983. Fish gamete preservation and spermatozoan physiology. Fish Physiology, IX B. pp. 305-350
19 Morisawa, M. and K. Suzuki. 1980. Osmolality and potassium ion: their roles in initiation of sperm motility in teleosts. Science, 210 : 1145-1147   DOI
20 Chang, Y.J., H.K. Lim and K.H. Kho. 1995. Properties of semen and sperm motility in black seabream, Acanthopagrus schlegeli. J. Aquaculture, 8 : 149-157
21 Kho, K.H., M. Morisawa and K.S. Choi. 2003. Membrane hyperpolarization increases cAMP to induce the initiation of sperm motility in Salmonid fishes, rainbow trout and masu salmon. J. Micro. Biotech., 13 : 833-840
22 Strussmann, C.A., P. Renard, H. Ling and F. Takashima.1994. Motility of pejerrey, Odontesthes bonariensis spermatozoa. Fish. Sci., 60 : 9-13   DOI
23 Ciereszko, A. and K. Dabrowski. 1993. Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture, 109 : 367-373   DOI   ScienceOn
24 Morisawa, M. and M. Okuno. 1982. Cyclic AMP induces maturation of trout sperm axoneme to initiate motility. Nature, 295 : 703-704   DOI   ScienceOn
25 Morisawa, M. and H. Hayashi. 1985. Phosphorylation of a 15 K axonemal protein is trigger initiating trout sperm motility. Biomed. Res., 6 : 181-184   DOI