• 원예과학기술지
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• 제19권2호
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• pp.159-162
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• 2001

한국에 자생하는 22지역의 바위솔속 식물들의 유전적 근연관계를 구명하기 위하여 RAPD분석을 실시하였다. 18종의 바위솔속 식물은 3개의 군(A, B, C 군)으로 집괴되었으며, 나머지 4종은 어떤 분류군에도 속하지 않았다. A군인 연화바위솔(No.18-No.22)은 유사도가 66.3-73.9%로 낮게 나타났으며, B군인 둥근바위솔(No.12-No.17)도 유사도 지수가 69.7-83.7%로 비교적 낮게 나타났다. C군인 바위솔(No.3-No.9)은 안면도 수집종(No.9)을 제외한 유사도 지수가 84.2-92.3%로 높아, 지역종 간에 변이가 적은 비교적 유전적으로 안정된 종으로 생각되었다.

• 한국자원식물학회지
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• 제9권3호
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• pp.230-238
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• 1996

재배장소(栽培場所)에 따른 야생(野生)더덕 및 재배(栽培)더덕의 기상환경(氣象環境), 생육특성(生育特性), 일반성분(一般成分), 유리(遊離) 아미노산(酸) 및 식물성(植物性) 정유성분(精油成分)을 비교분석(比較分析)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 자생지(自生地)는 재배지(栽培地에)에 비하여 기온(氣溫)이 $2{\sim}3^{\circ}C$ 지온(地溫)은 $2^{\circ}C$ 정도(程度)로 낮았고 조도(照度) 량(量낮)은 재배지(栽培地)의 2.9%였다. 자생지(自生地) 토양(土壤)의 pH는 재배지(栽培地)보다 다소 낮았으며 유기물(有機物) 함량(含量)은 4.8%로 1.7배(倍) 정도 높게 나타났다. 2. 자생지(自生地) 재배(栽培)더덕의 개화기(開花期)가 8월(月)3일(日)로 같았지만 재배지(栽培地) 야생(野生)더덕은 재배(栽培) 더덕 보다 10일(日) 늦은 8월(月)15일(日)이었다. 만장(蔓長), 엽장(葉長), 경경(莖徑) 등(等)은 재배(栽培) 장소(場所)와는 무관(無關)하게 야생(野生)더덕에서 저조(低調)하였으나 방향성(芳香性)은 평균(平均) 5로서 재배(栽培)더덕이 평균(平均) 3.5인 것에 비해 높은 것으로 나타났다. 3. 야생(野生)더덕의 생체중(生體重)은 평균(平均) 11g/주(株)인 것에 비하여 재배(栽培)더덕은 23.5g/주(株딘)였으나 방향성(芳香性)은 재배지(栽培地)에서는 차이(差異)가 없었으나 자생지(自生地)에서는 야생(野生)더덕이 5 정도(程度)로 다소 높은 경향(傾向)을 보였다. 4. 야생(野生)더덕과 재배(栽培)더덕의 재배장소별(栽培場所別) 일반성분(一般性分)의 조성(組成)은 조단백질(粗蛋白質)의 경우(境遇) 재배장소(栽培場所)에 따른 차이(差異)를 보였으며 자생지(自生地)보다 재배지(栽培地)에서 높은 함량(含量)을 보였고 조(粗)사포닌은 재배지(栽培地)보다 자생지(自生地)에서 높은 함량(含量)을 보였다. 5. 무기성분함양(無機成分含量)의 차이(差異)는 K의 경우(境遇) 자생지(自生地)에서 보다 재배지(栽培地)에서 평균적(平均的)으로 10배이상(倍以上) 정도(程度) 높은 함량(含量)의 차이(差異)를 보였으나 Mn, Zn, Na, Cu 등(等)은 일정(一定)한 경향(傾向)을 보이지 않는 것으로 나타났다. 6. 유리(遊離) 아미노산(酸)의 함량(含量)은 자생지(自生地은)보다 재배지(栽培地)에서 전반적(全般的)으로 높은 함량(含量)을 나타내었고, 특(特)히 Arginine은 다른 성분(成分)들과 비교(比較)해 볼 때 가장 높은 조성(組成)의 차이(差異)를 나타내었다. 7. 야생(野生)더덕과 재배(栽培)더덕의 정유성분수율(精油成分收率)은 자생지재배(自生地栽培)에서는 모두 0.004% 였고 재배지(栽培地)에서는 야생(野生)더덕이 0.005%였다. 8. 더덕의 재배장소(栽培場所)에 따른 향기성분(香氣成分)은 총(總) 21종(種)이었으며 自生地(自生地)에서 야생(野生)더덕은 16종(種), 재배(栽培)더덕은 18종(種)이었고, 재배지(栽培地)에서 야생(野生) 더덕은 14종(種), 재배(栽培)더덕은 20(種)이었다. 9. Trans-2-hexanol은 야생(野生)더덕의 자생지(自生地) 재배(栽培)에서 피이크 면적(面積) 당(當) 50.3%, 재배지(栽培地)에서 피이크 면적(面積) 당(當) 43.3%를 보였으며 amylalcohol, furfuryl acetate, 2-methoxy-4-vinyl phenol(MVP)는 재배(栽培)더덕에서만 확인(確認)되었다.

• Journal of Ecology and Environment
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• 제43권2호
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• pp.254-262
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• 2019

Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15-25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50-80% of the length of the seeds' development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L. insularis and L. harae germinated within 4 weeks after sowing at 15 ℃ and 20 ℃, respectively. Contrarily, L. praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 ℃, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 ℃, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 ℃). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 ℃) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 ℃, 20 ℃, 15 ℃, and 20 ℃, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.

• 한국환경복원기술학회지
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• 제7권2호
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• pp.75-86
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• 2004

This study aims to figure out ecological characteristic of natural forests focusing on vegetation as a way of ecological renovation for the restoration of naturalness for golf courses that were constructed in the Country and to present vegetation models and appropriate tree species for the purpose. The study site is P golf club, which is located in Gapyong-gun, Kyeonggi Province. The site is within a forest where the grade from the natural ecology map is the first one and the level from the green index accounts for the eight, thus showing a typical environment for a golf course in terms of location. The location of the site explains a reason for restoration. The major substance of the study is to conduct ecological evaluation of vegetation structures around and inside the golf course and to present a vegetation model. In order to evaluate the ecological characteristics of the vegetation structures, the analysis of the study covered succession stages, multi-layer vegetation structures and species diversity. Plant communities that have high species diversity were selected for the vegetation model and proper density and species were proposed considering the number of species and individuals and distances between trees. The vegetation restoration model targets succession into an oak forest. Within a unit of 100 $m^2$, the recommended model include a tall-tree layer with 11 trees such as Quercus serrata and Quercus mongolica, a sub-tall-tree layer with 12 trees including Quercus mongolica, Quercus serrata, Prunus sargentii, Fraxinus rhynchophylla and Acer pseudo sieboldianum, a shrub layer with 32 trees from 16 species, and a grass layer with a cover rate of 45 %. The proposed vegetation restoration model needs to apply to : 1) damaged natural forests by the construction of golf courses; 2) boundaries between golf courses and surrounding forests; 3) buffer zones; 4) open spaces in between courses; and 5) areas between greens and tees where open spaces are available in a mass. In conclusion, one of the most important factors in presenting a vegetation model for the restoration of naturalness in the golf course and other damaged forests is to provide multi-layer vegetation structures that are composed of native species. As for the specific application for the site, it is recommended to manage the vegetation in such a way that the environment of the site can have a similar environment to the surrounding forest which is expected to succeed into an oaks-dominant one.

• 한국균학회지
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• 제45권4호
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• pp.355-361
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• 2017

본 연구는 전라북도 진도에서 자란(Bletilla striata), 강원도 정선군 함백산에서 감자난초(Oreorchis patens)와 은대난초(Cephalanthera longibracteata)의 뿌리에서 내생균을 분리하고 이들의 형태 및 분자생물학적 방법을 통해 동정하였다. 각 균주의 형태학적 특징을 분석하기 위해 포자의 모양과 특징을 관찰하였고, 분자생물학적 동정을 위해 ITS1F와 ITS4 primer를 이용하여 internal transcribed spacer (ITS) 및large subunit (LSU) rDNA영역을 분석하였다. 그 결과 Phialocephala bamuru, Coniochaeta mutabilis, Phialophora foetens, Calonectria Canadensis, Neonectria ramulariae 5종의 국내 미기록 내생균이 동정되어 이를 보고하고자 한다.

• Animal Systematics, Evolution and Diversity
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• 제39권4호
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• pp.295-299
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• 2023

Two land snails, Aegista chejuensis (Pilsbry and Hirase, 1908) and Plectotropis quelpartensis (Pilsbry and Hirase, 1908), are endemic to Korea and were collected from Hataedo and Jodo Islands in the Yellow Sea of South Korea, respectively. Many terrestrial snail habitats have been confirmed in Korea; however, their genetic sequences have rarely been reported. This study describes the mitochondrial cytochrome c oxidase subunit I gene (COI) sequences of two species, followed by an analysis of the genetic distance between these two species and their congeners. As a result, there was no intra-species variation in both species A. chejuensis or P. quelpartensis. However, the inter-species variation was clear (10.3-31.5%). We provide photographs and a brief diagnosis for morphological verification.

• 한국약용작물학회지
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• 제17권2호
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• pp.125-132
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• 2009

This study was carried out to investigate the distribution of native Asparagus cochinchinensis and ecological characteristic in South Korea. Natural vegetative areas were investigated at 5 areas; Taean, Buan, Geoje, Namhae and Jindo. In this study, the $5m{\times}5m$ quadrat was established for recording coverage and appearance species by phytosociological method. The flora of the studied area in native habitats were listed as 130 species. The native habitats was classified into Pinus thunbergii community and typical community. Two communities were located in a coastal cliff and have been destroyed. Therefore Asparagus cochinchinensis native habitats must be protected by regulation. In the studied sites, soil pH, organic matter, nitrogen, available phosphorus, exchangeable K, exchangeable Ca, exchangeable Mg and cation exchange capacity were ranged from $5.1{\sim}5.7%$, $1.77{\sim}3.59%$, $0.19{\sim}0.54%$, $5.4{\sim}18.7$ (mg/kg), $0.24{\sim}0.48$ (cmol+/kg) $0.76{\sim}2.83$ (cmol+/kg), $3.11{\sim}6.22$ (cmol+/kg) and $8.7{\sim}24.5$(cmol+/kg), respectively.

• Food Science and Biotechnology
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• 제17권2호
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• pp.357-361
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• 2008

One of the major allergenic proteins in common buckwheat (Fagopyrum elculentum) was found to be a BW10KD. In this work, allergenic BW10KD genomic DNAs from the native common buckwheat 'Pyeongchang' and Tartarian buckwheat 'Clfa47' were cloned by polymerase chain reaction (PCR), and their nucleotide sequences were determined. In addition, a novel PCR assay targeting the allergenic BW10KD gene was developed to detect and differentiate both buckwheat species in food. The nucleotide sequences of the BW10KD genomic DNA from 'Pyeongchang' and 'Clfa47' were 94% identical. Base differences in the nucleotide sequences of the BW10KD genes are probably useful as a molecular marker for species-specific identification. The 'Pyeongchang'-specific primer set 154PF/400PR and the 'Clfa47'-specific primer set 154DF/253DR generated 247 and 100 bp fragments in singleplex PCR, respectively. A duplex PCR assay with 2 species-specific primer sets simultaneously differentiated the 'Pyeongchang' and 'Clfa47' in a single reaction. The PCR assay also successfully allowed for the rapid detection of buckwheat ingredients in foods.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.66-71
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• 1992

The ecological distribution of endomycorrhizas in evergreen woody species native to the evergreen forest ecosystem of Tadohae-haesang National Park in southern Korea in February, 1989 was studied. The abundance and diversity of vesicular-arbuscular (VA) mycorrhizal fungi were also determined. The spore densities ranged from 14 to 326 per 100 g of soil. Most of the spores of mycorrhizal fungi collected from 25 soil samples belonged to the genera Glomus and Gigaspora. The frequency and number of spores in Camellia japonica varied with location. Spores belonging to the genus Gigaspora were not found in Camellia japonica in Yesongri evergreen forests adjacent to the sea. Glomus sp. was the major constituent of the spore assemblage at this site. The most abundant species in Camellia japonica in the Yesongri evergreen forests in Pogildo was Glomus borealis. In the soil of a mountain at Buwhangri, in the central location of the island at an elevation of 250 m, Gigaspora sp. was present and Glomus sp. was a major constituent of the spore assemblage. In the urban area of Haenam spore densities were much higher than in the Pogildo area. The most abundant species in Camellia japonica in the urban area of Haenam was Gigaspora sp..

• 한국환경과학회지
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• 제31권7호
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• pp.579-592
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• 2022

Much controversy has been generated over whether the ecological restoration project in Korea is faithful to the basic approach of ecological restoration compared to projects aimed at creating parks or green spaces. This study was aimed at understanding the level of awareness of practitioners in the planting design method, which is recognized as relatively important in landscape architecture and ecological restoration. The proficiency of practitioners in planting design techniques for ecological restoration projects was investigated and analyzed in seven areas: overall planting design awareness, plant species selection method, planting tree sizes, planting density, use of native species, vegetation climatic zone, soil environment, and weed control. There was no statistically significant difference between the group who thought they were experts in the field of ecological restoration and the group who thought they were experts in the field of parks and green spaces. Moreover, there was no statistically significant difference between the degree of work performance, that is, the group whose main industry was ecological restoration, the group that performed relatively more ecological restoration work, and the group that rarely performed it. Therefore, to apply desirable ecological restoration techniques in the future and to clearly differentiate between the approach in the field of parks and green spaces, the appropriate tree size or planting density selected in the ecological restoration planting design stage must be chosen. To this end, the awareness of expert groups must be improved and related laws, systems, and guidelines must be revised.