• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.3
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• pp.86-95
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• 2004

This study was conducted to develop recycle revegetation methods for the restoration of the steep slopes by using fresh wood chip from construction sites. In general, the fresh wood chips can be used as soil media for the restoration works, because they can increase infiltration of rainfall and give enough porous to breathe and elongate for the root growth as well as economic value. The experiment was carried out to compare the effect of fresh wood chips from different mixing with soil, organic material and macromolecular compound which used for slope restoration works conducted by Hyunwoo green(Ltd.). The main results by monitoring for two years are summarized as follows; 1. The soil media made with low percentage of fresh wood chip covered quickly by herb plants. Especially, the soil mixture Type C (wood chip 20%) showed 80 percent ground coverage within two months after seeding. 2. The soil mixture type E (wood chip 40%) and type F (wood chip 50%) which contains more fresh wood chips than soil type C was under 30 percent ground coverage because wood plants are germinated well. If the restoration works aims at making forest, then the soil type E and F would be recommended than using soil type C. 3. Among the woody plants, Ailanthus alfissima, Pinus rigida, Pinus densiflora, and Albizzia julibrissin showed high percentage of germination rates and vigorous growth. In case of shrubs, Lespedeza cyrtobotria and Indigofera pseudo-tintoria scored high percentage of germination rates. 4. In native plants, Chrysanthemum indicum, Artemisia princeps, Lutos corniculatus and Imperata cylindrica showed high percentage of appearance. In case of introduced herbs, Coreopsis lanceolata, Coreopsis tinctoria and Oenothera oborata grew so vigorously. 5. The soil types which including fresh wood chips over 30-40 percentage showed the most diverse plant composition and the most effective germination rates and growth pattern with woody plants. 6. This works to develop recycle revegetation methods using fresh wood chips need more efforts for monitoring the exact effect of fresh wood chips as the soil media.

• Journal of Environmental Science International
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• v.23 no.5
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• pp.895-902
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• 2014

Bacterial cellulose (BC) has played important role as new functional material for food industry and industrial products based on its unique properties. The interest in BC from static cultures has increased steadily in recent years because of its potential for use in medicine and cosmetics. In this study, we investigated culture condition for BC production by Acetobacter sp. F15 in static culture. The strain F15, which was isolated from decayed fruit, was selected on the basis of BC thickness. The optimal medium compositions for BC production were glucose 7%, soytone 12%, $K_2HPO_4$ 0.2%, $NaH_2PO_4{\cdot}_2H_2O$ 0.2%, lactic acid 0.05% and ethanol 0.3%, respectively. The strain F15 was able to produce BC at $26^{\circ}C-36^{\circ}C$ with a maximum at $32^{\circ}C$. BC production occurred at pH 4.5-8 with a maximum at pH 6.5. Under these conditions, a maximum BC thickness of 12.15 mm was achieved after 9 days of cultivation; this value was about 2.3-fold higher than the thickness in basic medium. Scanning electron micrographs showed that BC from the optimal medium was more compact than plant cellulose and was reticulated structure consisting of ultrafine cellulose fibrils. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose.

• Weed & Turfgrass Science
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• v.6 no.4
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• pp.306-312
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• 2017

This study was carried out to develop new hybrid zoysiagrass cultivar 'Seah' (The application no. for cultivar protection : 2014-22). Native zoysiagrasses were collected from south-west seaside of Korea from 2010 to 2011. Artificial crossing was conducted to develop F1 hybrid between Z2011 (Z. sinica) and NM1 (Z. matrella) at plastic house in 2011. Among the progenies, 'Seah' showed fine leaf texture and high shoot density from the space planting plots at field. 'Seah' showed genetically light green color, with fine leaf with 1.8mm and height to the lowest leaf blade was 1.94 cm. Ground coverage rate was slower than medium leaf zoysiagrass (Jung-gi), but plant height of 7.1 cm was the lowest among the compared zoysiagrasses and height to lowest leaf of 1.94 cm was lower than most zoysiagrass, which may allow low mowing height.

• Journal of Environmental Science International
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• v.28 no.12
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• pp.1061-1069
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• 2019

Common reed (Phragmites australis) is widespread in reclaimed land and wetland habitats. Every year, the common reed produces extensive colonies by means of underground rhizomes and ground-surface stolons. From an agricultural point of view, the common reed's large biomass is a good material for supplying organic matter. However, it has not yet been studied in terms of seedling production, transplanting conditions, and decomposition characteristics in reclaimed land. Seeds were harvested from the native common reed in Saemangeum, South Korea, the previous year and stored on an open field. The seeds were sowed in the greenhouse at the beginning of April. Common reed decomposition was studied from June to September, with the use of coarse mesh (5 mm) stem litterbags, on three samping dates and with five replicate packs per sample. These packs were dug in five soil condition (low-salinity topsoil, subsoil, high-salinity topsoil, subsoil, paddy topsoil) to 0.2 m and 0.4 m depth. The highest germination rate of common reed seeds was observed in non-salt solution, but the exhibited germination rate was 70% at 9.38 dS m^-1. The plant height of young reed decreased steadily with increasing salinity, but leaf number did not decrease by 9.38 dS m^-1. The survival rate of the two-year-old reed was 83.3%, which was 35% higher than that of the one-year reed. The transplant success rate was 0% in the no vinyl mulching in the soil, but the first year and second year seedlings survived rates were 63% and 83.3%, respectively, in vinyl mulching. Common reed decomposition rates were faster low salinity than high salinity. All nutrient contents were found to fluctuate significantly with time by soil conditions. We also need to study the growth rate of reed transplanting seedlings by soil moisture contents and the comparison of degradation in common reed tissues.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.3
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• pp.29-43
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• 2012

This study evaluated 23 botanic gardens and arboretums under two main categories including professional group and operational group. As for professional group, the main difference between the functions of botanic gardens and arboretums was analyzed. In the case of operational group compared professional group respectively, on categories including importance of function and implementation, seeking to provide fundamental data of botanic gardens and arboretums by enhancing the quality of visitors and examining functional differences. The result of a difference in arboretums' function between professional and operational groups, the study analyzed the importance of botanic gardens and arboretums in terms of detailed categories such as research, education, collection, display, retention, and service for visitors. As a result, professional group showed retention 4.32>education 4.11>research 4.09>service for visitors 4.05, and operational group showed retention 3.70>service 3.52>education 3.41>research 3.37, indicating that both groups had retention as the most selected answer. Looking closely at the functions of the currently implemented detailed categories of botanic gardens and arboretums, such as research, education, collection, display, retention, and service for visitors, the study showed that 91.3% selected rare species and individual multiplication for research function, 78.3% chose education of natural ecosystem for children for education, 73.9% answered space for experiencing nature for display, and 73.9% also replied managon, diof rare species, reproduction, and retention of native plants' species for retention category, and service for visitors showed 95.7% for implementation. The study could not clearly point out the influence between predictors due to absence of an established comprehensive evaluation model. Also, it analyzed and reviewed comparison of functions of professional group and operational group, but in the future, it should conduct study on a systemic and objective classification of botanic gardens and arboretums to clearly examine the difference in views between groups, which also requires, at the same time, study on management policy of botanic gardens and arboretums or suggesting guidelines that suit types of forms, and also study on facilities and program development that can be applied to each class.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.49-57
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• 2015

This research was initiated to analyze the periods of spring green-up and winter leaf discoloration of three ornamental grasses which have potential to be widely used with seed propagation. Two native grasses of Arundinella (Arundinella hirta var. ciliata Koidz), fountaingrass (Pennisetum alopecuroides (L.) Spreng), and switchgrass (Panicum virgatum L.) was tested. Spring green-up were evaluated after one year growth from seed propagation on April 1, 2009. Arundinella started with quick pick of spring green-up during $13^{th}$ to $20^{th}$ of May. Fountaingrass and switchgrass showed relatively slow picks of green-up during $20^{th}$ to $27^{th}$ of May. However, winter leaf discoloration started on swtichgrass and fountaingrass but Arundinella terminated relatively slowly. Swtichgrass showed the pick discoloration during $8^{th}$ to $15^{th}$ of October from the bottom to top parts of the plant. Fountaingrass showed the pick winter discoloration started from bottom to top parts during the $22^{nd}$ to $29^{th}$ of October. However, Arundinella showed relatively slow discoloration from upper to bottom parts during October $29^{th}$ to November $5^{th}$. Arundinella showed a relatively higher ornamental value with 125 days of the complete green period compared than fountaingrass and swtichgrass which maintained approximately 105 days of green period.

• Korean Journal of Microbiology
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• v.45 no.3
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• pp.275-280
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• 2009

The optimal medium compositions for the production of bacterial cellulose (BC) by a Acetobacter sp. V6, which was isolated from the traditionally fermented vinegar in Korea, were investigated in static cultures. The optimum medium compositions for BC production were 3% glucose, 3% soytone, 0.8% $K_2HPO_4$, and 0.4% ethanol, respectively. Adding $NaH_2PO_4$ or $KH_2PO_4$ had not shown the increase in BC production. Under the optimum medium compositions, the highest BC production was 44.67 g/$m^2$ in 8 days and the thickness of BC pellicle was about 1 cm. Structural properties of BC produced in the optimal medium were studied using Fourier-transform infrared spectroscopy and X-ray diffractometer. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose. No difference in the compositions between bacterial and plant celluloses, but BC showed unique micro-network structure and high crystallinity (82%).

• Journal of Life Science
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• v.25 no.2
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• pp.189-196
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• 2015

Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

• Molecules and Cells
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• v.27 no.4
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• pp.423-428
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• 2009

Superoxide dismutases (SODs) constitute the first line of cellular defense against oxidative stress in plants. SODs generally occur in three different forms with Cu/Zn, Fe, or Mn as prosthetic metals. We cloned the full-length cDNA of the Thellungiella halophila Cu/Zn-SOD gene ThCSD using degenerate RT-PCR and rapid amplification of cDNA ends (RACE). Sequence analysis indicated that the ThCSD gene (GenBank accession number EF405867) had an open reading frame of 456 bp. The deduced 152-amino acid polypeptide had a predicted molecular weight of 15.1 kDa, an estimated pI of 5.4, and a putative Cu/Zn-binding site. Recombinant ThCSD protein was expressed in Escherichia coli and assayed for SOD enzymatic activity in a native polyacrylamide gel. The SOD activity of ThCSD was inactivated by potassium cyanide and hydrogen peroxide but not by sodium azide, confirming that ThCSD is a Cu/Zn-SOD. Northern blotting demonstrated that ThCSD is expressed in roots, stems, and leaves. ThCSD mRNA levels increased by about 30-fold when plants were treated with sodium chloride (NaCl), abscisic acid (ABA), and indole-acetic acid (IAA) and by about 50-fold when treated with UVB light. These results indicate that ThCSD is involved in physiological pathways activated by a variety of environmental conditions.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.134-142
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• 2008

This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).