• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.784-788
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• 2006

Microsatellite polymorphism and the genetic relationship were estimated using genotype information of 305 horses from 11 microsatellite loci. The breeds include the indigenous Korean breeds, Korean native horse (102) and Jeju racing horse (56) together with Japan Hokkaido horse (5), Mongolian horse (19), Thoroughbred horse (108), Quarter horse (11) and Przewalskii horse (4). Allelic frequencies, the number of alleles per locus were estimated by direct counting from observed genotype, and genetic variability was computed using the CERVUX software and DISPAN. The number of alleles per locus varied from 6 (HMS6) to 18 (ASB17) with an average value of 10.45 in horse breeds. The expected total heterozygosity ($H_T$) and coefficient of gene differentiation ($G_{ST}$) ranged 0.764-0.921 (the average value was 0.830) and 0.102-0.266 (the average value was 0.180) in horse breeds, respectively. Four populations (Przewalskii horse, Japan Hokkaido horse, Quarter horse, Thoroughbred horse) showed lower heterozygosity than the average value (the average value was 0.710). The expected heterozygosity within breed ($H_S$) and mean no. of observed alleles ranged from $0.636{\pm}0.064$ (Japan Hokkaido horse) to $0.809{\pm}0.019$ (Mongolian horse), and from 2.73 (Przewalskii horse) to 8.27 (Korean native horse), respectively. The polymorphic information content (PIC) ranged from 0.490 (Przewalskii horse) to 0.761 (Mongolian horse) with an average value of 0.637 in horse breeds. The results showed three distinct clusters with high bootstrap support: the Korean native horse cluster (Korean native horse, Mongolian horse), the European cluster (Przewalskii horse, Thoroughbred horse), and other horse cluster (Jeju racing horse, Japan Hokkaido horse, and Quarter horse). A relatively high bootstrap value was observed for the Korean native horse cluster and European cluster (87%), and the Korean native horse and Mongolian horse (82%). Microsatellite polymorphism data were shown to be useful for estimating the genetic relationship between Korean native horse and other horse breeds, and also be applied for parentage testing in those horse breeds.

• 생명과학회지
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• 제14권3호
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• pp.521-524
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• 2004

본 연구는 short oligonucleotide primer를 이용하여 마 품종간 유전 분석을 실시 하고자 PCR증폭 기법을 확립하고, 확립된 기술을 이용하여 제주도에 사육중인 천념기념물 347호로 등록된 제주말과 경주마로 잘 알려진 더러브렛간의 유전적인 다양성을 분석한 결과 마 품종간 차이를 보이는 DNA marker는 9개의 primer에서 확인되었으며, 이중 6개의 primer에서 더러브렛 특이 밴드와 나머지 3개에서 제주 마 특이 RAPD 밴드가 확인되어 cloning과 sequencing후에 SCAR primer를 제작하여 마 품종 식별에 활용할 수 있을 것으로 사료되며, 본 연구결과 RAPD표지인자는 마 품종간의 유전 분석에 매우 유용한 것으로 판단되었다.

• 생명과학회지
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• 제15권3호
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• pp.474-478
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• 2005

본 연구는 RAPD 기법을 이용한 종 특이 marker 개발 및 이 marker의 SCAR marker로의 개발을 목표로 수행되었다. Random primer 700개에 대하여 PCR 수행결과, 품종간, 개체간에 많은 다형성이 관찰되었으며 품종특이적인 양상을 나타내는 MG30, MG53의 primer는 각각 2.0kb, 2.3kb의 위치에서 제주말과 더러브렛종의 특이적인 RAPD 단편을 나타내었다. 이들 단편들 중 품종 특이적인 단편을 클로닝한 후 random primer가 포함된 부분의 염기서 열을 결정하였다. 10 bp의 RAPD random primer에 10bp의 염기를 추가하여 SCAR primer를 제작하였다. SCAR marker의 수행결과 RAPD marker와 같은 2.3kb, 2.0kb의 크기에서 제주마와 더러브렛종에 특이적인 하나의 밴드가 증폭되었다. 따라서 이 Cnh-SCAR marker는 보다 안정적이고 재현성 있는 marker로서 사용이 가능하여 제주말의 판별에 유용하게 사용될 수 있을 것이다.

• 생명과학회지
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• 제14권4호
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• pp.696-701
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• 2004

제주마의 기원 및 혈통보존을 위한 기초자료를 마련할 목적으로 국내에서 사육중인 210두(제주마 73두, 교잡마 118두, 몽고마 19두)의 말을 대상으로 적혈구항원형의 표현형 분포와 유전자 빈도를 조사한 결과를 요약하면 다음과 같다. 제주마, 교잡마, 그리고 몽고마 적혈구항원형의 표현형은 각각 Aa (27.4%, 63.6%, 63.2%), Ca (97.3%, 94.9%, 89.5%), K- (97.3%, 99.2%, 84.2%), Pa (39.7%, 44.9%, 42.1%), 그리고 Ua (71.2%, 70.3%, 63.2%)에서 가장 높은 빈도를 나타내었고 D 시스템의 유전자형과 Q 시스템의 표현형은 각각 Dbcm/dghm (12.3%), Dbcm/cgm (14.4%), Dcgm/dghm (15.8%)와 Qc (56.2%), Qabc (36.4%), Qc (31.6%)에서 높은 빈도로 관찰되었다. 적혈구항원형의 유전자 빈도의 경우 제주마는 A- (0.287), Ca (0.827), Ddghm (0.226), K- (0.985), Pa (0.358), Qc (0.494), U- (0.529), 교잡마는 Aa (0.529), Ca (0.776), Dbcm (0.306), K- (0.995), P-(0.531), Q- (0.504), U- (0.548), 몽고마는 Aa (0.421), Ca (0.895), Ddghm (0.421), K- (0.842), Pa (0.447), Qc (0.448), Ua (0.632) 대립유전자가 가장 높은 빈도를 보였으며 대립유전자 Dcfgk 와 D- 는 모든 말에서 관찰되지 않았다.

• 한국가축번식학회지
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• 제22권1호
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• pp.67-72
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• 1998

Nowadays many kinds of foreign breed horse and Korean native Cheju horse are raised in Korea. Horse has complicated structure and function to maintain normal health, and to overcome severe condition during gallop at full speed. Sometimes the disease take a bad turn becuase of difficulty of correct diagnosis for horse disease. In recent times, horse farm for fattening and reproduction is gradually increasing. However, thoracic disease and abdominal disease are frequently occuring by defect in raising management. But the diagnosis of these kinds of disease is difficult by former diagnostic method. Ultrasonographic diagnostic method is a method to slove the problem. In abroad, nowadays ultrasonography has been reported for circulatory organs and abdomonal organs in horse, cattle, sheep, and dog, and the importance of ultrasonographic diagnosis is recognized in veterinary clinical medicine. In the country, ultrasonography of Korean native goat, cattle and dog was recently reported, but there was no report for the ultrasonography of horse. So, ultrasonogram for the thyroid glands, jugular vein and carotid artery was evaluated in Cheju horse and Thoroughbred horse. Thyroid gland, jugular vein and carotid artery were determined on both sides of neck. The length of right thyroid lobe at the Thoroughbred horse, Thoroughbred foul, Cheju horse and Cheju foul were 54.8, 45.1, 46.1 and 42.2 mm, respectively. The width of right thyroid lobe at the Thoroughbred horse, Thoroughbred foul, Cheju horse and Cheju foul were 18.1, 14.3, 14.6 and 14.2 mm, respectively. The height of right thyroid lobe at the Thoroughbred horse, Thoroughbred foul, Cheju horse and Cheju foul were 27.3, 25.6, 26.2 and 25.5 mm, respectively. Similar ultrasonographic measurements were obtained for the left thyroid gland. The internal diameter of left jugular vein at the Thoroughbred horse, Thoroughbred foul, Cheju horse and Cheju foul were 10.6, 9.7, 10.5 and 9.2 mm, respectively. Similar ultrasonographic measurements were obtained for the right jugular vein. The internal diameter of right carotid artery at the Thoroughbred horse, Thoroughbred foul, Cheju horse and Cheju foul were 9.5, 8.8, 9.1 and 8.5 mm, respectively. Similar ultrasonographic measurements were obtained for the left carotid artery. It is concluded that the ultrasonographic values of this study provides references for the diagnosis of morphologic changes in the thyroid gland, jugular vein and carotid artery in Korean native Cheju horse and Thoroughbred horse.

• 생명과학회지
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• 제16권7호
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• pp.1109-1111
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• 2006

The present study was carried out to investigate the blood markers of the Korean native horse. A total number of 158 horses were tested using microhaemagglutination with 11D system reagents (Da, Db, Dc, Dd, De, Df, Dg, Dh, Dk, Dm and Dn). Of the 158 horses, 3 horses showed an unusual D system phenogroups; these phenogroups may be silent (null) alleles, De or Dk and Dc, respectively. Dacdfgm, Dacdfgmn, and Ddegmn phenogroups were recognized. These results present basic information for detecting the genetic markers among the Korean native and alien horses.

• 생명과학회지
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• 제17권10호
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• pp.1441-1446
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• 2007

본 연구는 서로 다른 상염색체에 위치하고 있는 초위성체유전 표지를 활용하여 제주마 집단의 타 품종과의 차별적 유전특성분석과 제주마 집단에서 활용할 수 있는 효율적인 개체 식별 시스템 설정을 위해 수행되었다. 공시재료로서는 5품종에서 총 191두가 사용되었으며 13종의 좌위에 대한 개체별 유전자형을 분석하였다. 이들 13종에서 출현된 총 대립 유전자수는 제주마의 경우 155종이 나타났다. 한국 제주마 집단에서 나타난 평균 이형접합도는 0.317-0.902였으며 marker 다형성 정보량은 0.498-0.799로 나타났다. 제주마 집단에서 나타난 대립 유전자 발현 특성은 다른 외래 품종 집단과 매우 상이한 결과를 나타냈다. ATH4 좌위의 경우 제주마 집단에서는 5종의 대립 유전자가 고른 분포를 나타낸 반면 QUA종의 경우와 THO종집단에서 특정 좌위의 극단적 발현 빈도를 나타냈다. 품종특이성을 나타내는 분자표지의 대립유전자 발현양상이 확인되었으며 이러한 품종특이성 발현 분자표지는 집단 내 개체에 대한 품종식별 유전자표지로 활용이 가능한 것으로 나타났다. 9종의 초위성체 유전 표지를 활용할 경우 누적 개체 식별력은 99.9%를 나타냈으며 두 마리의 서로 다른 개체가 서로 같은 유전자형을 가질 짝확률은 $0.60\;{\times}\;10^{10}$으로 추정되었다. 따라서 9종의 선정된 유전표지는 제주마 품종 집단에서 적정 신뢰도를 제공할 수 있는 개체 식별 시스템을 설정할 수 있을 것으로 생각된다.

• 한국임상수의학회지
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• 제26권3호
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• pp.220-225
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• 2009

말에서 개체식별 및 친자확인을 위한 ISAG microsatellite marker의 적용을 위한 유용성 및 표준화를 위해 더러브렛종 6두를 포함한 다양한 품종의 20두를 대상으로 22개의 ISAG microsatellite marker를 분석한 결과 대립유전자의 수는 4개에서 8개로 분포하였고 더러브렛종에서 출현하는 대립유전자의 대부분이 비더러브렛종에서도 출현하였다. 본 연구결과를 통해서 ISAG microsatellte marker를 제주말의 혈통등록에 적용할 수 있음을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.249-256
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• 2019

Objective: The present study aimed at comparing the nutritional composition and color traits between two meat types: Horse meat and pork from Korean native black pigs raised in Jeju Island. Methods: After slaughter 24 h, the longissimus dorsi samples were taken from left side carcasses of the 32-mo-old Jeju female breed horses and the 6-mo-old Korean native black pigs (n = 10 each). The samples were then placed into cool boxes containing ice packs and transported to the Laboratory of Meat Science where all visual fats and connective tissues were trimmed off and then the samples were ground. All the samples were analyzed for nutritional composition (proximate composition, minerals, vitamins, fatty acids, and amino acids) and color traits. Results: The horse meat contained significantly higher collagen, moisture and protein than the pork (p<0.05). The Jeju horse meat showed more desirable fatty acid profiles such as containing significantly lower saturated fatty acids (SFA), higher polyunsaturated fatty acids (PUFA) contents and PUFA/SFA ratios than the pork (p<0.05). Differences in concentrations of ten amino acids existed between the two meat types in which the horse meat had higher values for all these amino acids, total amino acids (20.33 g/100 g) and essential amino acids (10.06 g/100 g) than the pork (p<0.05). Also, the horse meat showed significantly higher concentrations of Fe (34.21 mg/100 g) and Cu (2.47 mg/100 g) than the pork (Fe, 17.42 mg/100 g and Cu, 1.51 mg/100 g) (p<0.05). All the vitamins detected showed statistical differences between the two meat types in which the horse meat had higher concentrations of vitamin B1 (25.19 mg/100 g), B2 (92.32 mg/100 g), B3 (2,115.51 mg/100 g), and B5 (67.13 mg/100 g) than the pork (p<0.05). Conclusion: Based on the results obtained in the study, it is concluded that the two meat types studied are rich in nutrients and the animal species strongly affected the nutritional values and color traits of the muscle tissues.

• 대한수의학회지
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• 제14권2호
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• pp.185-189
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• 1974

Although considerable research has been done on the blood chemistry of domestic animals, little work has been made of the changes associated with age. Moreover, the records about physiology of the goat were not much available in Korea, and a comprehensive survey of the blood chemical values of the Korean native goat has not been made. The authors intended to biometric study on the hardness of bone of race horse and Jeju horse in Korea. The measurement of hardness of bone were performed in 272 race horses (Thoroughbred 91, Anglo-Arab 107, Arab 74) and in 109 Jeju horses by the caliper by Toryba's method. Some interesting facts obtained through this study were as follows: 1. There was not significant difference of bone hardness between male and female. The average of bone hardness by the Toryba's meteod were $23.07{\pm}1.01$ in race horse and $19.44{\pm}1.84$ in Jeju horse. 2. The grade of bone hardness of race horse were higher than those of Jeju horse(P<0.001). 3. The correlation coefficient between age and grade of bone hardness were r= +0.344 in Jeju horse, theme were statistically significant (P<0.01) and the regression equation was Y=0.29x+18.497.