• Korean Journal of Poultry Science
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• v.43 no.2
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• pp.63-76
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• 2016

The genetic diversity of Korean native chicken is important for conservation of native chicken breeds and developing economically valuable traits in Korea. In this review, various types of genetic markers using Korean native chickens were investigated, which are mtDNA variations, microsatellite markers, markers in Major Histocompatibility Complex (MHC), and single nucleotide polymorphisms (SNPs). These genetic markers are suitable for breed discrimination and diversity studies because of their high polymorphism status. Thus, the purpose of this study was to summarize the genetic markers developed in the Korean native chickens and diversity studies using these breeds. Ultimately, these markers can be used for the future studies for understanding of genetic characteristics.

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.91.1-91.1
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• 2008

• Journal of Life Science
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• v.21 no.9
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• pp.1329-1335
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• 2011

Korean native goats, which are characterized by black coat color, have existed on the Korean peninsula for a long time. Until now, there has been no comprehensive investigation concerning their genetic diversity, phylogenetic analysis or origin. In this study, we investigated the genetic diversity and verified phylogenetic status of the Korean native goat using the 453-bp fragment of the hypervariable fragment I (HVI) of mitochondrial DNA (mtDNA) D-loop region from 60 individuals among 5 populations. The Korean native goat showed less haplotype diversity when compared with goats from other countries. In addition, 6 haplotypes that had not been previously reported were verified in this study. In phylogenetic analyses with other country's goats, 10 haplotypes from Korean native goats were classified into mtDNA lineage A. Moreover, in a phylogenetic tree for goats which contained mtDNA lineage A, 8 of 10 haplotypes could be included in a subgroup with goats from Vietnam and an area of China. However, none of the remaining haplotypes belonged to a major group of Korean native goats and were located on different independent positions. These results suggest that almost Korean native goats aligned more closely to China and Vietnam breeds in mtDNA lineage A and there was no gene flow from other mtDNA lineages. Our results will contribute to conservation strategies and genetic breeding of Korean native goats.

• Journal of Veterinary Clinics
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• v.20 no.2
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• pp.185-197
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• 2003

This survey was performed to investigate frequency of clinical disease in Holstein dairy cattle and Korean native cattle in Chungbuk region of Korea. This study was conducted with the veterinary clinical charts recorded by veterinarians in an animal hospital for 387 Holstein herds(14,701$\pm$1,766 head) and 180 Korean native cattle herds(1,440$\pm$786 head) from October 1998 to September 2001. The prevalent disease classes in Holstein dairy cattle were digestive systems(26.84%), reproductive system(22.48%), locomotor system(21.43%) and metabolic disorders(10.08%). The locomotor diseases were increased with 1st year, 16.27%, 2nd year, 20.93% and 3rd year, 27.08%. The dieases of digestive and reproductive system occurred frequently in winter season(30.01%, 26.03% respectively) and the disease frequency of locomotor system were very high in fall(28.44%). The predominant diseases of Holstein dairy cattle were : claw overgrowth(13.58%), indigestion(11.23%), functional infertility(8.77%), metritis(8.28%), foot diseases(7.35%), LDA (6.57%) and ketosis(5.76%). The prevalent disease classes in Korean native cattle were digestive system(43.80%), respiratory system(28.11%) and reproductive system(10.69%). Seasonally, digestive diseases occurred frequently in spring (51.55%), whereas respiratory diseases occurred frequently in winter (39.60%). The predominant diseases in Korean native cattle were calf diarrhea(32.03%), calf pneumonia(21.79%), indigestion(8.26%), dystocia(7.65%) and pneumonia(5.90%).

• Korean Journal of Agricultural Science
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• v.44 no.2
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• pp.244-253
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• 2017

This study was conducted to investigate the growth performance indices among male commercial crossbred Korean native chickens from hatch to twelve weeks of age. A total of 312 one-day-old male birds were used for 6 groups of the commercial crossbred Korean native chicken within 1 paternal line and 6 maternal lines (1A, 2A, 3A, 4A, 5A, and 6A). The chickens were allocated to 24 battery cages to give 4 replicates per each crossbreed with 13 chickens per cage. Ad-libitum feeding was practiced throughout the experimental period and fresh water was available via nipple drinkers at all times. Body weight and feed intake were measured at two-week interval. Among six crossbreeds, the 2A group had the greatest (p < 0.05) body weight at week 8 and the greatest average daily weight gain in week 6 - 8. However, crossbreed 2A had the lowest viability (p < 0.05) at week 8 of all crossbreed groups as well as higher uniformity (p > 0.05) at week 2 than the others. No difference (p < 0.05) was found in any crossbreed Korean native chicken's growth performance indices including body weight, average daily gain, average daily feed intake, feed efficiency, uniformity, and viability after week 8. With this in mind, crossbreed 2A had greater bodyweight, average daily gain, average feed intake, and feed efficiency than the other Korean native chickens 84 days after hatch, although they had lower uniformity and viability than the others.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.10
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• pp.446-451
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• 2019

This study investigated the application of artificial insemination and pregnancy diagnosis kit for Korean native black goats. Semen was collected by electrical ejaculation, followed by semen analysis and artificial insemination in three goat strains (Dangjin, Jangsu and Tongyoung). Pregnancy was confirmed using a cow pregnancy test kit (IDEXX Rapid Visual Pregnancy Test kit) and ultrasound diagnosis. Analysis revealed that semen collected from male Korean native black goats by electrical ejaculation was about 1~1.5 ml in volume, $18{\sim}25{\times}10^8/ml$ concentration, and having > 97% motility. Furthermore, confirmation of pregnancy by pregnancy test kit and ultrasound diagnosis after artificial insemination were similar. In addition, the efficiency of pregnancy was 20~40% for all three strains: Tongyoung was the highest with 44%, followed by Dangjin (%), and Jangsu (20%). This study determines the artificial fertilization efficiency and the feasibility of using a cow pregnancy test kit for early pregnancy diagnosis in Korean native black goats. Although further research is required for validation, the results of the current study contribute to the breeding and improvement of Korean native black goat in research institutions as well as in general farms.

• Journal of Embryo Transfer
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• v.23 no.2
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• pp.81-86
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• 2008

This study compared the pregnancy rates of Korean native donor cattle after either a timed artificial insemination (TAI) or embryo transfer (TET) following the synchronization of ovulation using a controlled internal drug release (CIDR) device together with estradiol benzoate (EB) and prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$). Fifty five cows and 8 heifers which had been previously used for embryo production were assigned to two treatments: (1) Thirty-two cattle received a CIDR device and 2 mg EB (Day 0), 25 mg $PGF_{2{\alpha}}$ injection at the time of CIDR removal on Day 7, and 1 mg EB injection on Day 8. All of the cattle received a TAI 30 h (Day 9) after the second EB injection (TAI group). (2) Thirty-one cattle received the same hormonal treatments as in the TAI group. The cattle with corpus luteum (CL) received a TET on Day 16 using frozen-thawed embryos (TET group). Ultrasonographic observations demonstrated that the proportion of cattle with synchronized ovulation on Day 10 and the concomitant formation of new CL on Day 13 did not differ between groups (p>0.05); the overall mean rates were 65.1 and 73.0%, respectively. The conception and pregnancy rates did not differ (p>0.05) between the TAI (12.5% and 12.5%) and TET groups (13.0% and 9.7%), respectively. We conclude that the pregnancy rate following TAI or TET in Korean native donor cattle was poor, which might be due in part to a poor synchrony of ovulation and concomitant CL formation.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.1-12
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• 2003

The present study was carried out to establish the standard karyotype of the Korean Native Chicken and to find their chromosomal band markers using high-resolution banding technique. Chromosome analysis was performed on early chick embryos following in vitro culture of fertilized eggs of the yellow-brown and the red-brown lines of the Korean Native Chicken which had been established at National Livestock Research Institute. The high-resolution banding of the chromosome was achieved by treating the embryos with ethidium bromide and colchicine during culture. On GTG-banding, the Korean Native Chicken exhibited a typical chick banding pattern in all the macrochromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Chicken were virtually identical to those of White Leghorn and International System for Standardized Avian Karyotypes(ISSAK). However, the lengths and G-band numbers of the Korean Native Chicken macrochromosomes were greater than those of White Leghorn and ISSAK. Especially in chromosomes 1 and Z, the Korean Native Chicken exhibited more separated bands in compared with ISSAK. In C-banding patterns, although a lot of observed cells had C-band polymorphic patterns, almost the Korean Native Chicken macrochromosomes had heterochromatic C-band on centromeres and/or near terminal part. However, the heterochromatic C-band was constantly observed at the end of q-arm of Z chromosomes and on the whole W chromosome. In addition, the Korean Native Chicken exhibited distinctive heteromorphic patterns of C-bands on the centromere of chromosome 3 and at the end of q-arm of Z chromosome between homologous chromosomes.

• Food Science of Animal Resources
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• v.25 no.1
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• pp.71-77
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• 2005

This study was carry out to investigate the quality characteristics of Korean native black ground pork compared with modern genotype ground pork during refrigerated storage. Korean native black pig and modern genotype pig were slaughtered at 75 kg and 105 kg of live weight, and for 240 days and 210 days of feeding periods, respectively. The ground lean pork (M. semimembranosus) was stored for 9 days at 4℃. The crude fat and crude protein contents were significantly (p<0.05) higher in Korean native black pork. The pH value after 5 days of storage was significantly (p<0.05) lower in Korean native black pork than in modern genotype pork. WHC of Korean native black pork was significantly (p<0.05) higher than that of modern genotype pork over time. The Korean native black pork maintained black reddish color because it had lower CIE L/sup */ value and higher CIE a/sup */ value than the modern genotype pork. CIE L/sup */, b/sup */, C/sup */ and h/sup O/ values decreased as storage time increased. TBARS (thiobarbituric acid reactive substance), POV (peroxide value) and FOX (ferrous oxidation xylenol orange) tended to increase as storage time increased in all of the groups, in particular, those values increased more rapidly in Korean native black pork. Total saturated fatty acid and stearic acid contents had significantly higher in Korean native black pork (p<0.05).

• Food Science of Animal Resources
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• v.30 no.1
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• pp.13-19
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• 2010

To investigate the differences in the quality characteristics between commercial Korean native chicken (KNC) and broiler (CB), nutritive and quality parameters of the two chicken species were determined. The KNC thigh muscle had a lower content of crude fat and higher crude ash than the CB thigh. In regards to the fatty acid composition, KNC breast muscle had a higher content of arachidonic acid (C20:4) than CB. The level of inosine was higher in the CB thigh muscle than KNC but there was little difference in other nucleotide compounds. The KNC breast had higher amounts of glycine, alanine, and proline than CB, which are closely related to high quality meat flavor. The sensory acceptance was not significantly different between the breast and thigh of KNC and CB. However, KNC had higher cohesiveness, chewiness and gumminess than CB, which are indicative of a unique texture property. Based on these results, commercial KNC may have superior nutritional quality, taste, and unique texture when compared with CB. Thus, the consumer preference for KNC may be partially explained by these distinctive quality characteristics.