• 한국약용작물학회지
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• 제27권1호
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• pp.17-23
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• 2019

Background: Cnidium officinale Makino and Ligusticum chuanxiong Hort. are important medicinal crops in Korea. However, there is insufficient information on the identification of thrips, which attack these plants. Until now, one species of thrips has been recorded as a main pest. Methods and Results: To identify the thrips emerging in C. officinale Makino and L. chuanxiong Hort., these plants were independently cultivated in two local areas. Thirty individuals of each plant species were selected randomly and surveyed for the presence of thrips. After confirming the existence of thrips, 100 thrips individuals were collected from each crop using the beating method. To identify thrips species, we performed PCR-restriction fragment length polymorphism (RFLP)-based analysis using ITS2 primer sets. Six thrips species were identified: western flower thrips (Frankliniella occidentalis), flower thrips (F. intonsa), onion thrips (Thrips tabaci), chrysanthemum thrips (T. nigropilosus), chilli thrips (Scirtothrips dorsalis), and grass thrips (Anaphothrips obscurus). The proportion of these species differed between the host plant species. Conclusions: Six thrips species were major pests of two medicinal crops. Integrated pest management is required to control these thrips species, and will enhance the yield and quality of C. officinale and L. chuanxiong.

• 치위생과학회지
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• 제7권3호
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• pp.197-200
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• 2007

본 연구는 생약의 추출분획 용매 중 헥산추출에 따른 우식원인균의 억제효과와 배양시간 경과에 따른 항균력을 시간별로 비교 검토함으로써 치아우식예방제재로서의 가능성을 살펴보고자 헥산 분획에 의한 생약추출물 11종을 대상으로 우식원인균인 S. sobrinus B13를 사용하여 항균활성을 측정하여 다음과 같은 결과를 얻었다. 생약 추출물에 S. sobrinus를 접종한 직후, 6, 12, 24시간 후의 항균효과를 측정해 보았을 때, 단삼이 가장 좋은 항균효과를 나타냈고, 그 다음으로 향부자와 목향, 백출 순으로 나타났다.

• 한국약용작물학회지
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• 제26권4호
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• pp.281-290
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• 2018

Background: Lung cancer, the most common malignant disease worldwide, is the predominant cause of cancer deaths, particularly amongst men. Therefore, various researchers have focused on the growth inhibitory effects of medicinal plants used in traditional Korean medicine. This study aimed to investigate the growth inhibitory effects of ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos on NCI-H1229 cells. Method and Results: The viability of NCI-H1229 cells was evaluated in vitro using an MTS assay. Treatment with the ethanol extracts of the selected medicinal plants at $500{\mu}g/m{\ell}$ reduced NCI-H1229 cell viability and increased apoptotic cell death and caspase-3 activation. In addition, treatment with ethanol extracts of Inulae flos and Astilbe radix increases DNA fragmentation, as measured by the TUNEL assay. Conclusions: These results indicated that ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos exhibited growth inhibitory effects, inducing apoptotic cell death, DNA fragmentation and caspase-3 activation in NCI-H1229 cells. Therefore, these medicinal plant extracts may be used in the development of natural medicines to inhibit the growth of lung cancers. However, further study is needed to determine the active ingredients of the ethanol extracts from medicinal plants that are reposible for the inhibitory effect on lung cancer cell grwoth.

• Toxicological Research
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• 제11권2호
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• pp.309-313
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• 1995

The aim and objective of this study are to carry out the liver protective activities against the $CCl_4$ intoxication in mice with some Iranian medicinal plants traditionally used for liver injuries. The methanol extracts of Cichorium intybus, Lactuca scarJoia, Eucalyptus camadulensis were evaluated. With various doses of these plants, liver protective activities were performed after $CCl_4$ administration to mice. The serum aminotransferases activites, liver sizes, and histopatological examinations of liver were studied. At a dose of 50 mg/ kg, all three plants were able to protect liver damages induced by $CCl_4$.

• Mass Spectrometry Letters
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• 제14권3호
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• pp.79-90
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• 2023

Natural goods, especially therapeutic plants, are abundant in the World. Because they have the ability to provide all humanity with countless advantages as a source of medicines, medicinal plants are presently receiving more attention than ever. These plants' therapeutic efficacy is based on bioactive phytochemical components that have clear physiological effects on the human body. The drying process is crucial for the preparation of plant materials prior to extraction since freshly harvested plant materials include active enzymes that create active components, intermediates, and metabolic processes. Many of the phytoconstituents may be extracted using the semi-polar solvent methanol. The goal of the current work is to use the GC-MS gas chromatography- mass spectrometry technology to identify the phytochemicals and review their biological activity. In methanol leaf extract, 5 phytocompounds were found in Ricinus communis, 5 phytocompounds in Pongamia pinnata, 12 phytocompounds in Datura metal, 7 phytocompounds in Azadirachta indica, 11 phytocompounds in Acalypha indica.

• Natural Product Sciences
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• 제9권2호
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• pp.73-79
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• 2003

To discover the sources with antioxidative activity in traditional medicines, 100 extracts of Korean medicinal plants were screened for their scavenging effect on peroxynitrite $(ONOO^{-})$ and total reactive oxygen species (ROS). The potency of total ROS scavenging activity was shown in the extracts of 25 plants, and 4 of their species, Macleaya cordata R. Br., Salvia plebeia R. Br., Cassia tora L. and Angelica gigas Nakai, had a greater effect with $IC_{50}$ values of $1.7{\pm}0.36$, $4.3{\pm}1.08$, $4.9{\pm}0.17$ and $5.8{\pm}1.01\;{\mu}g/ml$, respectively, than that of trolox, positive control $(7.61{\pm}0.12\;{\mu}g/ml)$. Another 35 extracts exhibited inhibitory effect of below 50 percent at $100\;{\mu}g/ml$ of sample concentrations on total ROS, while the rest observed total ROS generators rather than scavengers. The peroxynitrite scavenging activities were observed in the greater part of the plants tested. Five of them, Schisandra chinensis Baill, Campsis grandiflora (Thunb.) K. Schum., Cedrela sinensis A. Juss., Pleuropterus multiflorus Turcz. and Veronica linariaefolia Pall represented scavenging activities on peroxynitrite twice as strong with $IC_{50}$ Values of $0.48{\pm}0.10$, $0.59{\pm}0.15$, $0.60{\pm}0.10$, $0.64{\pm}0.10$ and $0.91{\pm}0.23\;{\mu}g/ml$, respectively, as that of penicillamine $(1.72{\pm}0.05\;{\mu}g/ml)$, positive control. Consequently, 25 species of the entire plants tested, exhibited scavenging activities on total ROS and $ONOO^{-}$, Salvia plebeia R. Br., Macleaya cordata R. Br., Cassia tora L. and Angelica gigas Nakai exerted potent scavenging activities on both radicals.

• 한국약용작물학회지
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• 제20권6호
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• pp.466-471
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• 2012

'Usul' is a traditional medicinal herb, which has anti-inflammatory activities is distributed in India, Nepal, China, Korea, Japan. Korea pharmacopeia listed 'Usul' as either a species of Achyranthes japonica (Miq.) Nakai or A. bidentata Blume. Recent taxonomic studies in China and Japan delimited these taxa as two varieties, A. bidentata Blume var. bidentata and var. japonica Miq. A multivariate morphometric study of Achyranthes bidentata complex was undertaken to assess the entities of taxa that usefully could be recognized. Five quantitative characters were reviewed and analyzed with 293 specimens from Korea. The univariate analysis of inflorescence length, interval between florets, angle between floret and floral axis indicated that ranges among all taxa were continuous. However, quantitative characters of membrane size and the number of hairs within 4 were useful to identify two varieties. In PCA, the first three principal components accounted for 89.4% of the total variance. PCA revealed that var. bidentata showed distinctions in morphological attributes from var. japonica entity. Therefore, continued recognition at the infraspecifc level for these taxa is supported.

• 한국자원식물학회지
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• 제30권6호
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• pp.623-639
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• 2017

We investigated traditional knowledge about ethnobotanical uses around national parks in the northern areas of Province Gyeongsangbuk-do, with the focus on the Sobeaksan National Park area. Interviews were carried out to 138 residents at 42 places from 7 counties and/or cities, and verified species and usage information were categorized by taxonomic groups, usage, and used parts. The ethnobotanical species of the regions consisted of a total of 277 taxa: 244 species, 3 subspecies, 28 varieties, and 2 forma in 213 genera of 79 families. The significant native plants included one Endangered Species (Paeonia obovata Max.) and four endemic species (Salix hallaisanensis H. Lev., Aconitum pseudolaeve Nakai, Paulownia coreana Uyeki, and Cirsium setidens (Dunn) Nakai, Sasa coreana Nakai). About 17 Approved Species for Delivering Overseas designated by Korea Ministry of Environment have been also traditionally valuable in the regions. The main usage of the plants were edible (185 taxa) and medicinal (175 taxa). Leaves were most commonly used parts (109 taxa), followed by stems (73 taxa), fruits (69 taxa), roots (67 taxa), and wholes (57 taxa). The traditional usage of plants were diverse as those useful parts. Leaves, fruits and seeds were used as edible and medicinal; sprouts (young shoots) were mainly used as edible; but roots and flowers were used as medicinal. The study does not only provides examples of traditional uses of native plants, but also facilitates sustainable managements and developments of ethnobotanical knowledge for the contemporary society.

• 한국토양비료학회지
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• 제48권4호
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• pp.278-284
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• 2015

In general, plant roots accumulate more heavy metals than the above ground organs such as leaf, stem, and fruit. This implies that root medicinal plants would be an issue with excessive heavy metal accumulation. Therefore, the current study was carried out to investigate the distribution of heavy metal (focused on Cd and Pb) concentrations in soils and medicinal plant roots grown in different region of Korea. Total 293 samples for each soil and plant were collected along the national wide. Soil pH, total and phytoavailable metal concentrations (1 M $NH_4NO_3$ extracted) in soils were determined and heavy metal concentrations in root of the medicinal plants were analyzed. Heavy metal concentrations of the soil samples studied were not exceeded standard limits legislated in 'Soil Environmental Conservation Act', except 2 samples for Cu. However, substantial amount of Cd was accumulated in medicinal plant roots with 29% samples exceeding the standard limit legislated in 'Pharmaceutical Affairs Act' while all plant samples were lower than the standard limit value for Pb. Also the current study demonstrated that cadmium concentrations in the roots were governed by the phytoavailable Cd in soils, which decreased as soil pH increased. From this result, application of heavy metal immobilization technique using a pH change-induced immobilizing agents can be suggested for safer root medicinal plant production.

• 한국자원식물학회지
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• 제30권1호
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• pp.58-77
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• 2017

본 연구는 노추산(정선군, 강릉시)지역에 대하여 2014년 4월부터 2015년 10월까지 총 7회에 걸쳐 관속식물 분포를 조사하였다. 전체 조사된 분류군은 89과 304속 448종 4아종 57변종 6품종으로 총 515분류군이 자생하는 것으로 확인되었다. 주요 식물로 한국특산식물이 11분류군, 산림청지정 희귀식물 중 위기종이 1분류군, 취약종이 3분류군, 약관심종이 10분류군, 환경부지정 식물구계학적 특정식물 중 4등급종이 11분류군, 3등급종이 30분류군으로 확인되었다. 귀화식물은 총 24분류군으로서 귀화율은 4.6%, 도시화지수 7.5%로 나타났다. 그밖에 대한약전 및 대한약전외한약(생약)규격집에 수재된 약용식물은 67분류군으로 조사되었다.