• Journal of Wetlands Research
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• v.3 no.1
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• pp.1-17
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• 2001

The flora, vegetation structure, distribution of the vascular plants, physico-chemical characteristics of sediment, and nutrient contents of palnts were investigated in the ten disturbed and undisturbed wetlands around the littoral zone of Namgang, Jinju-si, Gyongsangnam-do, Korea from August 7 to August 15 in 1999. 217 taxa of the vascular plants distributed into 2 formas, 26 varieties, 189 species belong to 142 genera under 52 families were sampled or recorded. Dominant species of the undisturbed and the disturbed area were Persicaria thunbergii and Ambrosia artemisiifolia var. elatior, of which importance values were 13.86, and 15.81, respectively. Among 26 species of the naturalized plants, importance value of Ambrosia artemisiifolia var. elatior was highest as 9.54. The ranges of species diversity indices (H') were 1.370~1.819, and highest in the site 1 of the undisturbed area. The ranges of eqitability (J') were 0.779~0.902, and highest in the site 8 of the disturbed area. The ranges of community similarity indices ($CC_s$) were 0.341~0.578 among the ten wetlands, and highest between site 1 and site 2 which were the undisturbed area. The ranges of sediment properties such as pH, conductivity, organic matter, total phosphorus, and total nitrogen were 5.17~7.35, $24.67{\sim}74.33{\mu}mhocm^{-1}$, 1.09~3.82%, 0.0008~0.0034 mg/100g, and 0.06~0.15%, respectively. Nutrient contents of plants such as total nitrogen and total phosphorus were 1.52~2.65% and $0.010{\sim}0.039mg100g^{-1}$, respectively. It was recommended that the establishment of the conservation area was necessary to ensure the habitat for wild life and to maintain the diversity of the vascular hydrophytes and hygrophytes.

• Microbiology and Biotechnology Letters
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• v.37 no.2
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• pp.160-169
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• 2009

Poly-$\gamma$-glutamic acid ($\gamma$-PGA) was mixed natural flora of Bacillus subtilis, contaminated from cooked soybeans. Also, it was performed to find out the antiallergic activity by using NC/Nga mice, in vitro. The $\gamma$-PGA (PGA-HM : PGA-high molecular weight), Molecular weight 300 kDa, was decomposed and made PGA-LM (PGA-low molecular weight) which has molecular weight below 30 kDa by sonication. Therefore, it was same result between PGA-HM and PGA-LM, and reported PGA-LM as basic result. We found that PGA-LM contains antiallergic efficacy that inhibit B cells and Th2 cells activation from isolated CD4+T cells in NC/Nga atopic dermatitis model mice, and not show a cytotoxicity in the hFCs. To investigate the effects of these PGA-LM in vitro, isolation of splenic B cell and CD4+ T cells in atopic dermatitis mice were used. To elucidate the role of PGA-LM in anti-CD40+ interleukin-4 (IL-4)-mediated B-cell activation, showed that the capacity of B cells to expression IL-$1\beta$, IL-6, and TNF-$\alpha$ mRNA down-regulated, and IL-10 mRNA up-regulation by PGA-LM treatment, but it had no effect on TGF-$\beta$ expression. In addition to CD4+IFN-$\gamma$+ and CD4+CD25+foxp3+, the functions of PGA-LM in the development of the CD4+CD25+foxp3+ and CD4+IFN-$\gamma$+cells, the phenotype and functions of PGA-LM induced CD4+CD25+foxp3+, and CD4+IFN-$\gamma$+cells in CD4+T cells. These results suggested that PGA-LM could change cytokine production and generate CD4+CD25+foxp3+ Tregs in NC/Nga mice, and may be effective for immunotherapy in patients with AD.

• Journal of Food Hygiene and Safety
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• v.29 no.3
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• pp.189-194
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• 2014

This study was conducted to evaluate microorganism contamination of food utensils and service facilities in school and to prevent hazards by food poisoning occurrence. As a result, the highest number of microorganism growth plate ($12.3{\pm}2.6$) was detected in total bacteria test plate, and also observed $10.3{\pm}3.9$ growth plates in Staphylococcus aureus test plate and $9.5{\pm}3.9$ growth plates in E. coli and coliform bacteria test plate. But we could detect to the lowest number of growth plates ($1.5{\pm}1.0$) in Vibrio test plate. We also assessed that floors were appeared to the highest microorganism contamination rate in food utensils and service facilities. Therefore, $4.5{\pm}0.6$ growth plates was detected in pre-operation floor and $4.3{\pm}1.0$ growth plates in floor. And high level of microorganism contamination also observed in tables as $3.3{\pm}1.0$ growth plates in cooking table and $3.0{\pm}0.0$ growth plates in dining table. The level of microorganism contamination of food utensils such as kitchen knife, cutting board, and food tray were lower than that in food service facilities. We analysed microorganism contamination according to purpose of use in kitchen knifes and cutting boards. The microorganism contamination rate in fish kitchen knife ($2.0{\pm}0.8$) and fish cutting board ($1.3{\pm}1.5$) were slightly higher than that of others purpose of use. As a result of microorganism identification, various strains of microorganism were contaminated in food service facilities and some strains could detected more than two times. Especially, Staphylococcus aureus was repeatedly identified in cooking table, trench, and kitchen knife. Bacillus cereus was identified in kitchen knife, and then Pseudomonas fluorescens and Pseudomonas aeruginosa were also detected in food utensils and service facilities as known to food spoilage microorganisms. Klebsiella pneumoniae was detected four times repeat, which widely distribute natural environment as normal bacterial flora but sometimes cause acute pneumonia. These results suggest that food utensils and service facilities are contaminated with not only major food poisoning microorganisms such as Staphylococcus aureus, but also food spoilage microorganisms. Taken together, strict personal hygiene control and efficient food service facilities management will be needed to enhance food safety in school feeding and to improve student health.

• Applied Biological Chemistry
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• v.14 no.2
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• pp.121-129
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• 1971

During the Brewing of Soy Sauce on an industrial scale, Saccharomyces rouxii $T_9$ was cultured and added to the soy sauce mash. The comparative experiments of soy sauce mash in non-addition and addition group of yeast were examined in this report. The yeast flora and chemical composition of Soy sauce mash through out the brewing were observed and the results obtained were as follows. (1) The number of Osmophilic yeast in one ml of soy sauce mash showed $185{\times}10^3$ 1 month after mashing and $750{\times}10^3$ 4 months after mashing in case of yeast group, while presented as $98{\times}10^3$ 1 month after mashing and $394{\times}10^3$ 4 months after mashing in case of non-yeast group. And the number of Osmphilic yeast in yeast group was twice of that in non-yeast group. (2) The number of ordinary yeast of TTC red group was shown as $2132{\times}10^3\;to\;3252 ×10^3$ 5 to 6 months after mashing in case of yeast group, while presented $752{\times}10^3\;to\;1251{\times}10^3$ in case of non-yeast group. And the yeast group was shown more than non-yeast group in ordinary, red pink and pink yeast number. (3) TTC red yeast were strongly appeared in both addition and non-addition group of yeast from 1 month after mashing to 6 months after mashing. (4) Though total nitrogen, pure extract, pH and buffer action contents of soy sauce showed similiar tendency in yeast and non-yeast group, alcohol and color density contents were highly appeared in yeast group and reducing sugar content was in non-yeast group respectively. (5) By the results of Organic function test of soy sauce mash, the difference of taste quality in yeast and non-yeast group were not evidently appeared, however, the appearance and flavour of the soy sauce were better in yeast group than in non-yeast group.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.289-296
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• 2002

A broiler experiment was conducted to investigate the effect of supplementing yeast culture (Saccharomyces cerevisiae, Pichia pastoris) on the growth performance, small intestinal microflora and immune response in broiler chickens. One thousand hatched broiler chickens(Ross$^{(R)}$) were assigned to 6 treatments: control (basal diet), CTC; chlorotetracycline 100ppm, YC-SC; yeast culture(Saccharomyces cerevisiae) 0.3%, YC-PP; yeast culture(Pichia pastoris) 0.3%, RPPC-0.1; refined Pichia pastoris culture 0.1%, RPPC-0.3; refined Pichia pastoris culture 0.3%. There were no significant differences in growth, feed intake, feed efficiency and mortality among the treatments. However, chickens fed diets with yeast cultures showed numerically higher weight gain than those fed the control diets. Supplementation of yeast cultures and CTC improved feed efficiency and decreased mortality compared to control. Nutrient digestibilities were not affected by the dietary treatments. Total number of Lactobacilli in small intestine was higher while that of Cl. perfringens was lower with yeast culture treatments than control. Small intestine E. coli population of RPPC-0.3 treatment was significantly lower than that of the control. The serum IgG concentration tended to be higher in broilers fed yeast cultures than those fed the control and CTC diet. In conclusion, the supplementation of yeast culture products showed, although not significant but, numerical advantages in productivity and profile of microbial flora and serum IgG compared to the control and CTC supplementation.

• Journal of Wetlands Research
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• v.7 no.1
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• pp.129-138
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• 2005

The flora and vegetation of Jang-cheok wetland (Gyeong-nam) was investigated from April 1. 2004 to Feburary 28. 2005. The vegetation plants collected from this area were 18 taxa : 18 species 17 genera 14 families. Hygrophtes were 2 species 2 genera 2 families. Emergent plants were 6 species 5 genera 6 families. Submersed plants were 1 species 1 genera 1 families. Floating-leaved plants were 5 species 5 genera 5 families. Free-floating plants were 2 species 3 genera 2 families. Dominance of life form was investigated Trapa japonica, Phragmites commnis, Leersia japonica by 5 dominance values and Hydrocharis dubia, Ceratophyllum dmersum were 1 dominance values. The community was investigated 5 communities : Leersia japonica community. Trapa japonica community. Nelumbo nucifera community. Miscanthus sacchariflorus community. Phragmites communis community. Dominance species(Leersia japonica) of Leersia japonica community was investigated $1,89g/m^{2}$(Dry weight) and $1,730ind./m^{2}$(Density). Doninance species(Trapa japonica) of Trapa japonica community was investigated $36,25g/m^{2}$(Dry weight) and $15.20ind./m^{2}$(Density). Dominance species(Nelumbo nucifera) of Nelumbo nucifera community was investigated $30.59g/m^{2}$(Dry weight) and $11.20ind./m^{2}$(Density). Dominance species(Miscanthus sacchariflorus) of Miscanthus sacchariflorus community was investigated $180.50g/m^{2}$(Dry weight) and $124.80ind./m^{2}$(Density). Dominance species(Phragmites communis) of Phragmites communis community was investigated $159.50g/m^{2}$(Dry weight) and $60.00ind./m^{2}$(Density). The predominant species of this area was investigated Trapa. Japonica and the other communities was only small area in the waterside area.

• Journal of Oral Medicine and Pain
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• v.33 no.2
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• pp.159-166
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• 2008

An ideal anti-bacterial medication for oral infection requires selective effect on pathogens causing dental caries and periodontal disease but not on normal flora. In addition, it should be less toxic for human and even for environment. This study was to seek such a natural anti-bacterial medication and thus anti-bacterial effect of Hamamelis virginiana was evaluated. Many recent researches on the anti-bacterial effect of natural plant extract and essential oil have reported that natural products can be used as medication for prevention and restrainment of dental caries, halitosis and periodontitis. It has been also reported that Hamamelis virginiana has anti-bacterial effect on Porphyromonas gingivalis, Fusobacterium nucleatum, Capnocytophaga gingivalis, Veilonella parvula, Eikenella corrodens, Peprostreptococcus micros, and Actinomyces odontolyticus. This study evaluated anti-bacterial effect of Hamamelis virginiana on Streptoccoccus mutans, Haemophylus actinomycetemcomitans, and Klebsiella pneumoniae to expand its anti-bacterial effect on other important oral pathogens and eventually to develop its oral care products or apply to clinical purpose. In this study, anti-bacterial tests for antibiotic disk susceptibility, minimal inhibitory concentration and minimal bactericidal concentration were performed to evaluate anti-bacterial effect of Hamamelis virginiana against Streptoccoccus mutans, Haemophylus actinomycetemcomitans, and Klebsiella pneumoniae. The results showed that Hamamelis virginiana has anti-bacterial effect on all pathogen strains tested in this study and furthermore Hamamelis virginiana possesses bactericidal effect other than bacteriostatic effect on Streptoccoccus mutans, Haemophylus actinomycetemcomitans, Klebsiella pneumoniae. This study indicates that a natural anti-bacterial medication for oral diseases can be developed using Hamamelis virginiana.

• Journal of Dairy Science and Biotechnology
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• v.33 no.1
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• pp.1-15
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• 2015

Before the advent of pasteurization and other microbiological controls in the dairy industry, milk product-borne diseases such as scarlet fever, typhoid fever, septic sore throat, and tuberculosis were globally widespread. Pasteurization was invented by Louis Pasteur, and it has been considered as one of the most effective ways to control milk product-borne diseases since the 20th century. Nevertheless, till date, various types of cheese in the EU, the USA, and other countries are made from unpasteurized milk as artisan cheese, following the specific food regulations of each nation. Furthermore, after the effectiveness of the Free Trade Agreement (FTA) negotiations between Korea and many nations, the import of various types of cheese made in different conditions and influenced by acidity, preservatives, temperature, competing flora, water activity, and salt concentration increased yearly. Hence, the objective of this review was to describe (1) the 60-day aging rule of cheese, (2) characteristics of the outbreaks linked to cheese manufactured from unpasteurized milk since 1998 to 2011 in USA, and (3) serious health risks from unpasteurized milk, to ensure food protection and safety and to use this basic information for risk assessment.

• Journal of Oral Medicine and Pain
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• v.35 no.2
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• pp.101-110
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• 2010

Helicobacter pylori(H. pylori) is bacterial infection, with more than half of the world population infected and oral cavity is considered second reservoir of H. pylori infection. The purpose of this study was to evaluate role of oral cavity in H. pylori infection by comparison of the mode H. pylori infection in oral cavity and stomach. We recruited 100 subjects without systemic disease including gastrointestinal disease. Samples in oral cavity taken on gingival sulcus fluid(GSF) of lower left central incisor and 1st molar, area of buccal mucosa, dorsum of the tongue, palatal and saliva. We analyzed by Nested polymerase chain reaction(PCR) for oral infection and Urea Breath Test(UBT) for gastric infection. The results were as follows : 1. Among these 100 subjects, 36(36%) were positive by Nested PCR and 33(33%) were positive by UBT(p>0.05). 2. In detection rate of H. pylori in sites taken sample, 11(11%), 8(8%), 9(9%), 3(3%), 9(9%), 7(7%) were positive on GSF of lower left central incisor and 1st molar, area of buccal mucosa, dorsum of the tongue, palatal and saliva, respectively. Statical significance was observed in samples of GSF of lower left central incisor and area of dorsum of the tongue(p<0.05). 3. In comparison of the mode of H. pylori infection in oral cavity and stomach by analytic method, positive in oral cavity and stomach was 10(10%), negative in oral cavity and positive in stomach was 23(23%), positive in oral cavity and negative in stomach was 26(26%) and negative in oral cavity and stomach was 41(41%)(p>0.05). Conclusively, we can guess that oral H. pylori is not associated with gastric H. pylori infection and normal flora.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.4
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• pp.29-41
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• 2015

Abandoned paddy field provides an excellent opportunity to improve the species diversity and habitat quality. Ecological characteristic on the changing of plant communities at different seral stages is a major basis data for ecological restoration. In this study, we investigated changes of the species composition and community indices on the plant community associated with abandonment of cultivated rice paddies. The ecological stability of the habitat was evaluated by using eco-floristic characters(Di; Disturbance index, AUI; Actual urbanization index). Survey sites were grouped into six stages(stageI (${\leq}3years$), stageII(3-5years), stageIII(5-7years), stageIV(7-10years), stageV(10-15years), stageVI(${\geq}20years$). Vegetation investigation was done from May 2009 to October 2012 and carried out phytosociological approach. The total flora were summarized as 176 taxa including 58 families, 127 genera, 157 species, 3 subspecies, 15 varieties and 1 forms. At each of successional stages, 64 taxa in stage I, 34 taxa in stage II, 84 taxa in stage III, 83 taxa in stage IV, 92 taxa in stage V, 23 taxa in stage VI were identified. Of the occurrence plants, the species with the highest r-NCD value were Alopecurus aequalis, Juncus effuusus var. decipiens, Persicaria thunbergii, Artemisia princeps, Salix koreensis and Alnus japonica at each stages. Herbaceous annual plants were dominated in the early stage, but its r-NCD value declined in the middle stage and the late stage. On the other hand, herbaceous perennial plants and Persicaria thunbergii, annual hydrophytes, increases in the middle stage. Woody plant and herbaceous plant which appeared in the forest edge increases in the late stage. Community indices correlate with successional stages. Richness and diversity index increase along the successional gradient. But dominance index decrease along the successional gradient. Evenness index was correlated with lower. In the ecological stability analysis of the habitat that evaluated by eco-floristic characters, stage I was the most unstable habitat. And the stability of the habitat has improved according to the successional stage.