• Korean Journal of Childcare and Education
• /
• v.8 no.2
• /
• pp.27-61
• /
• 2012

The purpose of this study was to develop an integrated early childhood music education program using storytelling songs and to examine its possible effects by analyzing musical expression ability, language expression ability and creativity. In order to verify the effect of the program developed in the study, 68 4-year old preschoolers were selected from 2 preschools to be divided into an experimental group and a control group. The experimental group was given the integrated music education program 16 times in total (two times a week for 8 weeks). The data were analyzed by t-test. The results of this study were : First, it was found that the integrated early childhood music education program using storytelling songs had a positive effect on children's musical expression ability and linguistic expression ability. Second, it was found that the integrated early childhood music education program using storytelling songs had a positive influence among the sub-areas of creativity.

• Journal of the Korean Home Economics Association
• /
• v.45 no.2
• /
• pp.133-145
• /
• 2007

The purpose of this research was to identify the importance of emotional development in early childhood, in children ages three to five, by examining the relationship between the variables in the children such as gender, age, and temperament, as well as their mothers' emotionality, in relation to emotional development. The participants included a total of 72 children between three and five years of age. The major findings are as follow: First, there were significant differences in emotional expression and emotional recognition between the boys and the girls. Additionally, the emotional recognition of the children increased as age increased, and more positive strategies for emotional regulation were used with the increasing age of the children. Temperament characteristics did not have any relationship with emotional expression or emotional recognition, while the strategies for emotional regulation were related to the temperament characteristics. Second, the emotional expressivity of the mother was related to the emotional expression and recognition of the child, but wes not associated with strategies for emotional regulation. The emotional reactivity of the mother was related to a child's strategies for emotional regulation, but not to emotional expression or recognition. Third, emotional development of the children wes influenced by the individual child variables and emotionality of the mother.

• Research in Community and Public Health Nursing
• /
• v.20 no.3
• /
• pp.351-360
• /
• 2009

Purpose: The purpose of this study was to develop a lecture on oral presentation and to evaluate changes in anger expression, assertive behavior, and self-esteem after lecture on oral presentation. Methods: The design was a nonequivalent control group pretest-posttest design. The participants were university students in nursing, of which 17 were assigned to the experimental group and 15 to the control group. The education was carried out for 2 hours, once a week for 14 weeks. Data was analyzed with SPSS/WIN 14 program, and included chi-square test, independent t-test, and repeated measure ANOVA. Results: Lecture on oral presentation showed significant differences in the changes of anger expression (F=10.122, p=.003) and assertive behavior (F=8.498, p=.007). Conclusion: Findings suggest that this lecture on oral presentation was effective in changing students' anger expression and assertive behavior. Therefore this lecture on oral presentation is recommended for inclusion in education for university students in nursing.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.7
• /
• pp.1056-1069
• /
• 2015

Osmotic pressure is a critical factor for erythritol production with osmophilic yeast. Protein expression patterns of an erythritol-producing yeast, Yarrowia lipolytica, were analyzed to identify differentially-expressed proteins in response to osmotic pressure. In order to analyze intracellular protein levels quantitatively, two-dimensional gel electrophoresis was performed to separate and visualize the differential expression of the intracellular proteins extracted from Y. lipolytica cultured under low (3.17 osmol/kg) and high (4.21 osmol/kg) osmotic pressures. Proteomic analyses allowed identification of 54 differentially-expressed proteins among the proteins distributed in the range of pI 3-10 and 14.4-97.4 kDa molecular mass between the osmotic stress conditions. Remarkably, the main proteins were involved in the pathway of energy, metabolism, cell rescue, and stress response. The expression of such enzymes related to protein and nucleotide biosynthesis was inhibited drastically, reflecting the growth arrest of Y. lipolytica under hyperosmotic stress. The improvement of erythritol production under high osmotic stress was due to the significant induction of a range of crucial enzymes related to polyols biosynthesis, such as transketolase and triosephosphate isomerase, and the osmotic stress responsive proteins like pyridoxine-4-dehydrogenase and the AKRs family. The polyols biosynthesis was really related to an osmotic response and a protection mechanism against hyperosmotic stress in Y. lipolytica. Additionally, the high osmotic stress could also induce other cell stress responses as with heat shock and oxidation stress responses, and these responsive proteins, such as the HSPs family, catalase T, and superoxide dismutase, also had drastically increased expression levels under hyperosmotic pressure.

• Anatomy and Cell Biology
• /
• v.56 no.4
• /
• pp.508-517
• /
• 2023

In cancer patients, chemo/radio therapy may cause infertility by damaging the spermatogenesis affecting the self-renewal and differentiation of spermatogonial stem cells (SSCs). In vitro differentiation of stem cells especially mesenchymal stem cells (MSCs) into germ cells has recently been proposed as a new strategy for infertility treatment. The aim of this study was to evaluate the proliferation and differentiation of SSCs using their co-culture with Sertoli cells and conditioned medium (CM) from adipose tissue-derived MSCs (AD-MSCs). Testicular tissues were separated from 2-7 days old neonate Wistar Rats and after mechanical and enzymatic digestion, the SSCs and Sertoli cells were isolated and cultured in Dulbecco's modified eagle medium with 10% fetal bovine serum, 1X antibiotic, basic fibroblast growth factor, and glial cell line-derived neurotrophic factor. The cells were treated with the CM from AD-MSCs for 12 days and then the expression level of differentiation-related genes were measured. Also, the expression level of two major spermatogenic markers of DAZL and DDX4 was calculated. Scp3, Dazl, and Prm1 were significantly increased after treatment compared to the control group, whereas no significant difference was observed in Stra8 expression. The immunocytochemistry images showed that DAZL and DDX4 were positive in experimental group comparing with control. Also, western blotting revealed that both DAZL and DDX4 had higher expression in the treated group than the control group, however, no significant difference was observed. In this study, we concluded that the CM obtained from AD-MSCs can be considered as a suitable biological material to induce the differentiation in SSCs.

• Journal of Animal Science and Technology
• /
• v.63 no.4
• /
• pp.934-953
• /
• 2021

Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

• Proceedings of the Zoological Society Korea Conference
• /
• 1998.10b
• /
• pp.284.1-284
• /
• 1998

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
• /
• 1996.10a
• /
• pp.160.1-160
• /
• 1996

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
• /
• 1999.10b
• /
• pp.268.1-268
• /
• 1999

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
• /
• 1999.10b
• /
• pp.179.2-179
• /
• 1999

No Abstract, See Full Text